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Literature summary for 1.1.1.1 extracted from
- Identification and characterisation of two allelic forms of human alcohol dehydrogenase 2 (2002), Cell. Mol. Life Sci., 59, 552-559.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 5-7 genes encoding ADH, DNA and amino acid sequence determination and analysis, polymorphism and allelic frequencies analysis, gene ADH2 possesses 2 allelic forms with Ile308 or Val308, expression of ADH2 alloenzymes in Escherichia coli | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics, MW 25 kDa | Homo sapiens | |
| 0.009 | - |
Octanol | recombinant allozyme Ile308, pH 7.5, 25°C | Homo sapiens |  |
| 0.011 | - |
retinol | recombinant allozyme Ile308, pH 7.5, 25°C | Homo sapiens | |
| 0.012 | - |
retinol | recombinant allozyme Val308, pH 7.5, 25°C | Homo sapiens | |
| 0.016 | - |
Octanol | recombinant allozyme Val308, pH 7.5, 25°C | Homo sapiens | |
| 0.063 | - |
NAD+ | recombinant allozyme Ile308, pH 7.5, 25°C | Homo sapiens | |
| 0.074 | - |
NAD+ | recombinant allozyme Val308, pH 7.5, 25°C | Homo sapiens | |
| 9 | - |
ethanol | recombinant allozyme Ile308, pH 7.5, 25°C | Homo sapiens | |
| 10.6 | - |
ethanol | recombinant allozyme Val308, pH 7.5, 25°C | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
alcohol dehydrogenase 2, 2 allelic forms | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant ADH2 alloenzymes from Escherichia coli by DEAE and AMP or blue Sepharose chromatography, and ultrafiltration | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanol + NAD+ | - |
Homo sapiens | acetaldehyde + NADH | - |
? | |
| octanol + NAD+ | - |
Homo sapiens | octanal + NADH | - |
? | |
| retinol + NAD+ | - |
Homo sapiens | retinal + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | structure modelling | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADH | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
Octanol | recombinant allozyme Ile308, pH 7.5, 25°C | Homo sapiens | |
| 0.04 | - |
Octanol | recombinant allozyme Val308, pH 7.5, 25°C | Homo sapiens | |
| 0.087 | - |
retinol | recombinant allozyme Ile308, pH 7.5, 25°C | Homo sapiens | |
| 0.11 | - |
retinol | recombinant allozyme Val308, pH 7.5, 25°C | Homo sapiens | |
| 0.167 | - |
ethanol | recombinant allozyme Ile308, pH 7.5, 25°C | Homo sapiens | |
| 0.175 | - |
ethanol | recombinant allozyme Val308, pH 7.5, 25°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
kinetic analysis assay at | Homo sapiens |
| 10 | - |
standard assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Homo sapiens | |
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