Application | Comment | Organism |
---|---|---|
synthesis | enzyme can be used in preparative scale enantioselective oxidation of sec-alcohol in asymmetric reduction of ketones, using acetone and 2-propanol, respectively, as cosubstrates for cofactor-regeneration via a coupled-substrate approach | Rhodococcus ruber |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 1 tightly bound ion per subunit | Rhodococcus ruber |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Rhodococcus ruber |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | 80% v/v, stable at | Rhodococcus ruber |
Acetone | 50% v/v, stable at | Rhodococcus ruber |
additional information | chemotolerant enzyme | Rhodococcus ruber |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus ruber | - |
strain DSM44541 | - |
Rhodococcus ruber DSM 44541 | - |
strain DSM44541 | - |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus ruber |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a primary alcohol + NAD+ = an aldehyde + NADH + H+ | sequential reaction mechanism | Rhodococcus ruber |
Storage Stability | Organism |
---|---|
very stable during storage | Rhodococcus ruber |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alcohol + NAD+ | medium chain sec-alcohols or (omega-1)-ketones, no activity with primary alcohols or aldehydes | Rhodococcus ruber | aldehyde or ketone + NADH | - |
r | |
alcohol + NAD+ | medium chain sec-alcohols or (omega-1)-ketones, no activity with primary alcohols or aldehydes | Rhodococcus ruber DSM 44541 | aldehyde or ketone + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Rhodococcus ruber |
Synonyms | Comment | Organism |
---|---|---|
medium-chain secondary alcohol dehydrogenase | - |
Rhodococcus ruber |
sec-ADH A | - |
Rhodococcus ruber |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | - |
Rhodococcus ruber |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life: 35 h | Rhodococcus ruber |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
reduction | Rhodococcus ruber |
9 | - |
oxidation | Rhodococcus ruber |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Rhodococcus ruber | |
NADH | dependent on | Rhodococcus ruber |