Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli using a rhamnose-inducible system | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31997 | - |
x * 31997, amino acid sequence calculation | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
strain DSM 50106, stereoselective enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetophenone + NADH | the enzyme exhibits high stereoselectivity in the desymmetrization of the prochiral ketone acetophenone, producing optically pure (R)-alpha-phenyl ethanol at high conversion | Pseudomonas fluorescens | (R)-alpha-phenyl ethanol + NAD+ | - |
? | |
cyclohexanone + NADH | best cyclic ketone substrate | Pseudomonas fluorescens | cyclohexanol + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31997, amino acid sequence calculation | Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas fluorescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Pseudomonas fluorescens |