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Literature summary for 1.1.1.1 extracted from

  • Rella, R.; Raia, C.A.; Pensa, M.; Pisani, F.M.; Gambacorta, A.; de Rosa, M.; Rossi, M.
    A novel archaebacterial NAD+-dependent alcohol dehydrogenase. Purification and properties (1987), Eur. J. Biochem., 167, 475-479.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA 15 mM, 85% inhibition Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
Anisaldehyde
-
Saccharolobus solfataricus
0.013
-
benzyl alcohol
-
Saccharolobus solfataricus
0.019
-
propan-1-ol
-
Saccharolobus solfataricus
0.021
-
2-Methylcyclohexanol
-
Saccharolobus solfataricus
0.05
-
pentan-2-ol
-
Saccharolobus solfataricus
0.055
-
butan-1-ol
-
Saccharolobus solfataricus
0.225
-
pentan-3-ol
-
Saccharolobus solfataricus
0.25
-
(+/-)-3-methylcyclohexanone
-
Saccharolobus solfataricus
0.26
-
ethanol
-
Saccharolobus solfataricus
0.35
-
butan-2-ol
-
Saccharolobus solfataricus
0.5
-
propan-2-ol
-
Saccharolobus solfataricus
1.3
-
Cyclopentanone
-
Saccharolobus solfataricus
6.6
-
acetone
-
Saccharolobus solfataricus
7.5
-
3-methylbutan-2-one
-
Saccharolobus solfataricus
14
-
butan-2-one
-
Saccharolobus solfataricus
27
-
methanol
-
Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc 2 mM required for optimal activity Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
2 * 37000, SDS-PAGE Saccharolobus solfataricus
70000
-
gel filtration, sucrose density gradient centrifugation Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.92
-
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylcyclohexanol + NAD+
-
Saccharolobus solfataricus (+)-2-methylcyclohexanone + NADH + H+
-
r
3-methylbutan-2-one + NADH
-
Saccharolobus solfataricus 3-methylbutan-2-ol + NAD+
-
?
3-methylcyclohexanol + NAD+
-
Saccharolobus solfataricus 3-methylcyclohexanone + NADH
-
r
3-methylcyclohexanone + NADH
-
Saccharolobus solfataricus 3-methylcyclohexanol + NAD+
-
r
acetone + NADH
-
Saccharolobus solfataricus isopropanol + NAD+
-
r
anisaldehyde + NADH + H+
-
Saccharolobus solfataricus anisic alcohol + NAD+
-
?
benzyl alcohol + NAD+
-
Saccharolobus solfataricus benzaldehyde + NADH
-
?
butan-2-ol + NAD+
-
Saccharolobus solfataricus butan-2-one + NADH
-
?
butan-2-one + NADH
-
Saccharolobus solfataricus butan-2-ol + NAD+
-
r
butanol + NAD+
-
Saccharolobus solfataricus butyraldehyde + NADH
-
?
cyclopetanone + NADH + H+
-
Saccharolobus solfataricus cyclopentanol + NAD+
-
?
ethanol + NAD+
-
Saccharolobus solfataricus acetaldehyde + NADH + H+
-
?
methanol + NAD+
-
Saccharolobus solfataricus formaldehyde + NADH + H+
-
r
pentan-2-ol + NAD+
-
Saccharolobus solfataricus 2-pentanone + NADH
-
?
pentan-3-ol + NAD+
-
Saccharolobus solfataricus 3-pentanone + NADH
-
?
propan-2-ol + NAD+
-
Saccharolobus solfataricus acetone + NADH
-
?
propanol + NAD+
-
Saccharolobus solfataricus propionaldehyde + NADH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 37000, SDS-PAGE Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 95 continous increase in activity from 40°C to 95°C Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
24 h, 30% loss of activity Saccharolobus solfataricus
60
-
half-life: 20 h Saccharolobus solfataricus
70
-
half-life: 5 h Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.317
-
acetone
-
Saccharolobus solfataricus
0.983
-
Anisaldehyde
-
Saccharolobus solfataricus
1.02
-
Cyclopentanone
-
Saccharolobus solfataricus
1.22
-
3-methylbutan-2-one
-
Saccharolobus solfataricus
1.68
-
benzyl alcohol
-
Saccharolobus solfataricus
1.88
-
ethanol
-
Saccharolobus solfataricus
1.93
-
butan-2-one
-
Saccharolobus solfataricus
1.97
-
butan-1-ol
-
Saccharolobus solfataricus
2.17
-
3-methylcyclohexanone
-
Saccharolobus solfataricus
2.92
-
pentan-3-ol
-
Saccharolobus solfataricus
3.1
-
methanol
-
Saccharolobus solfataricus
3.55
-
butan-2-ol
-
Saccharolobus solfataricus
3.82
-
pentan2-ol
-
Saccharolobus solfataricus
4.03
-
propan-1-ol
-
Saccharolobus solfataricus
6.92
-
propan-2-ol
-
Saccharolobus solfataricus
7.72
-
3-methyl-cyclohexanol
-
Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
reduction of anisaldehyde Saccharolobus solfataricus
8.5
-
oxidation of benzyl alcohol Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Saccharolobus solfataricus
NADH
-
Saccharolobus solfataricus