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(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O
?
(acetyloxy)(phenyl)acetic acid + H2O
(S)-mandelic acid + (2R)-(acetyloxy)(phenyl)ethanoic acid
(R)-1,2-O-isopropylidene glycol acetate + H2O
(R)-1,2-O-isopropylidene glycol + acetate
-
-
-
-
?
(R,S)-1,2-O-isopropylidene glycol butyrate + H2O
(R)-1,2-O-isopropylidene glycol + butyrate
-
fresh bacterial cells or purified EST1 (0.01 micromol p-nitrophenol/min), 28°C, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol
low enantioselectivity (enantiomeric ratio E: 2.5 for EST1, E: 2.5 for whole cells)
-
?
(R,S)-1,2-O-isopropylidene glycol caproate + H2O
(R)-1,2-O-isopropylidene glycol + caproate
-
fresh bacterial cells or purified EST1 (0.01 micromol p-nitrophenol/min), 28°C, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol
no enantioselectivity by EST1 (enantiomeric ratio E: 1) compared to E: 2.8 for whole cells
-
?
1-naphthol acetate + H2O
1-naphthol + acetate
1-naphthyl acetate + H2O
1-naphthol + acetate
1-naphthyl butyrate + H2O
1-naphthol + butyrate
36% activity compared to 1-naphthyl acetate
-
-
?
1-phenyl-1-(trifluoromethyl)prop-2-yn-1-yl acetate + H2O
1-phenyl-1-(trifluoromethyl)prop-2-yn-1-ol + acetate
-
determination of enantiopreference, wild-type R enantioselectivity is 42
spectrophotometric assay coupling released acetate to NADH generation
-
?
13-(6,6'-diacetyl)sophorosyloxydocosanoic acid + H2O
?
Candida bororiensis
-
-
-
-
?
2-benzylcyclohex-1-en-1-yl acetate + H2O
2-benzylcyclohexanone + acetate
2-ethylcyclohex-1-en-1-yl acetate + H2O
2-ethylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product
-
-
?
2-isopropylcyclohex-1-en-1-yl acetate + H2O
2-isopropylcyclohexanone + acetate
-
isoenzyme Est I, 10% conversion of substrate with R-configuration of product
-
-
?
2-methylcyclohex-1-en-1-yl acetate + H2O
(S)-2-methylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product
-
-
?
2-n-pentylcyclohex-1-en-1-yl acetate + H2O
2-n-pentylcyclohexanone + acetate
2-n-propylcyclohex-1-en-1-yl acetate + H2O
2-n-propylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% R-configuration of product
-
-
?
2-naphthol acetate + H2O
2-naphthol + acetate
2-naphthyl acetate + H2O
2-naphthol + acetate
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
2-t-butylcyclohex-1-en-1-yl acetate + H2O
2-t-butylcyclohexanone + acetate
3,6-diacetylmorphine + H2O
morphine + acetate
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
3-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
3-O-methyl-D-glucopyranoside + vinyl acetate
6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol
4-acetoxybenzoic acid + H2O
4-hydroxybenzoate + acetate
-
-
-
-
?
4-methylumbelliferone + H2O
?
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
?
4-methylumbelliferyl acetate + H2O
?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
4-nitrophenyl beta-D-glucopyranoside + vinyl acetate
4-nitrophenyl 3-O-acetyl-beta-D-glucopyranoside + vinyl alcohol
-
yield is 70.2%
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenyl D-xylopyranose
4 mM substrate, position-specificity, hydrolysis activity is specific for 3-, and 4-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside, 2-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside is a bad substrate
beta-xylosidase-coupled assay
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
4-nitrophenyl formate + H2O
4-nitrophenol + formate
5 min, 30°C, pH 5.8
-
-
?
4-nitrophenyl hexanoate + H2O
4-nitrophenol + hexanoate
-
-
-
-
?
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl-alpha-L-arabinofuranoside + acetate
-
-
-
-
?
4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
preferred substrate
-
-
?
4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl-alpha-L-arabinofuranoside + acetate
-
-
-
-
?
4-nitrophenyl-butanoate + H2O
4-nitrophenol + butanoate
-
-
-
-
?
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
4-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
6-acetylmorphine + H2O
morphine + acetate
6-O-acetyl-D-mannopyranose + H2O
?
-
-
-
-
?
6-O-acetyl-D-mannopyranoside + H2O
?
-
-
-
-
?
7-aminocephalosporanic acid + H2O
?
-
-
-
-
?
7-aminocephalosporanic acid + H2O
deacetylated 7-aminocephalosporanic acid + acetate
-
-
-
-
?
9-O-acetyl sialic acid + H2O
sialic acid + acetate
-
-
-
?
acetyl alginate + H2O
alginate + acetate
-
-
-
?
acetyl xylan + H2O
xylan + acetate
-
from beech wood or oat spelt
-
?
acetylated hemicellulose + H2O
? + acetate
-
the best substrate of the enzyme is 3-O-acetylated Xylp residue followed by 4-O-acetylated Xylp residue with a free vicinal hydroxyl group
-
-
?
acetylglucuronoxylan + H2O
glucuronoxylan + acetate
-
the enzyme deacetylates singly and doubly acetylated xylopyranosyl residues in the polymer to such an extent that it results in the polymer precipitation
-
-
?
acetylhemicellulose + H2O
hemicellulose + acetate
-
-
-
-
?
acetylxylan + H2O
xylan + acetate
-
60°C, pH 6, acetyl xylan esterase (acetate-releasing) activity on birchwood xylan
-
-
?
alpha,alpha-trehalose + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
an acetic ester + H2O
an alcohol + acetate
-
-
-
-
?
apigenin 7-O-(6-O-malonylglucoside) + H2O
?
-
-
-
-
?
arabinogalactan + H2O
?
-
-
-
-
?
astragaloside I + H2O
astragaloside IV + ?
astragaloside II + H2O
astragaloside IV + ?
beechwood xylan + H2O
?
-
-
-
-
?
beta-naphthyl acetate + H2O
beta-naphthol + acetate
birchwood xylan + H2O
?
-
-
-
-
?
bovine submaxillary mucin + H2O
deacetylated bovine submaxillary mucin + acetate
-
-
-
?
cellobiose + vinyl acetate
? + vinyl alcohol
cellobiose octaacetate + H2O
?
-
low activity
-
?
cellulose acetate + H2O
cellulose + acetate
cephalosporin C + H2O
deacetylated cephalosporin C + acetate
-
-
-
-
?
cephalosporin C + H2O
deacetylcephalosporin C + acetate
-
-
-
-
?
chlorogenic acid + H2O
?
-
-
-
-
?
D-galactose + vinyl acetate
? + vinyl alcohol
D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate
? + vinyl alcohol
D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate
? + vinyl alcohol
D-glucose + vinyl acetate
6-O-acetyl-D-glucopyranose + vinyl alcohol
D-glucose penta-acetate + H2O
?
D-mannose + vinyl acetate
6-O-acetyl-D-mannopyranose + vinyl alcohol
D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
diacetin + H2O
?
-
-
-
-
?
ethyl acetate + H2O
ethanol + acetate
geraniol acetate + H2O
geraniol + acetate
-
enzyme plays a role in geraniol production and improving palmarosa oil quality during florescence development
-
?
geranyl acetate + H2O
geraniol + acetate
-
-
-
?
glucose penta-acetate + H2O
?
-
high activity
-
-
?
glucose pentaacetate + H2O
?
glycerol triacetate + H2O
?
isoamyl acetate + H2O
isoamyl alcohol + acetate
-
25°C, 1 h, pH 7, 7 mM MgCl2, cell extracts
-
-
?
isoastragaloside I + H2O
astragaloside IV + ?
isoastragaloside II + H2O
astragaloside IV + ?
kojic acid + vinyl acetate
7-O-acetyl-kojic acid + vinyl alcohol
-
yield is 30.9%
-
-
?
malonylated isorhamnetin 3-O-glucoside + H2O
?
-
-
-
-
?
methyl 2,3,4-O-triacetyl-beta-D-xylopyranoside + 3 H2O
methyl beta-D-xylopyranoside + 3 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 2,3,4-O-triacetyl-beta-D-xylopyranoside + H2O
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + 2 H2O
methyl beta-D-xylopyranoside + 2 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O
methyl 2-O-acetyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + 2 H2O
methyl beta-D-xylopyranoside + 2 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + H2O
?
methyl 2-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
methyl xylotriose + acetate
-
-
-
-
?
methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + 2 H2O
methyl beta-D-xylopyranoside + 2 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + H2O
?
methyl 3-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
methyl xylotriose + acetate
-
-
-
-
?
methyl 4-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
methyl xylotriose + acetate
-
-
-
-
?
methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
methyl 6-O-acetyl-beta-D-glucopyranoside + H2O
?
methyl acetate + H2O
methanol + acetate
methyl alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
methyl beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
methyl beta-D-glucopyranoside + vinyl acetate
methyl 3-O-acetyl-beta-D-glucopyranoside + vinyl alcohol
-
yield is 56.4%
-
-
?
methyl-beta-D-xylopyranoside + vinylacetate
methyl-beta-D-xylopyranoside monoacetate + vinyl alcohol
transacetylation of methylxyloside in aqueous solution (40°C, pH 6, 25 mM methyl-beta-D-xylopyranoside)
analysis by thin-layer chromatography
-
?
monoacetin + H2O
glycerol + acetate
-
-
-
-
?
naphthyl acetate + H2O
naphthol + acetate
-
-
-
-
?
naphthyl butyrate + H2O
naphthol + butyrate
-
-
-
-
?
naphthyl propionate + H2O
naphthol + propionate
-
-
-
-
?
O-acetyl-1,4-beta-xylooligosaccharide + H2O
1,4-beta-xylooligosaccharide + acetic acid
O-acetyl-4-O-methyl-D-glucurono-D-xylan + H2O
4-O-methyl-D-glucurono-D-xylan + acetic acid
O-acetyl-galactoglucomannan + H2O
?
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
p-nitrophenyl butyrate + H2O
p-nitrophenol + butyrate
-
-
-
-
?
p-nitrophenyl decanoate + H2O
p-nitrophenol + decanoate
-
-
-
-
?
p-nitrophenyl hexanoate + H2O
p-nitrophenol + hexanoate
p-nitrophenyl laurate + H2O
p-nitrophenol + laurate
p-nitrophenyl propionate + H2O
p-nitrophenol + propionate
penta-O-acetyl-alpha-D-glucose + H2O
?
-
complete and regioselective removal of primary acetyl-group in 6-position
-
-
?
phenyl acetate + H2O
phenol + acetate
poly(vinylacetate) + H2O
? + acetate
-
-
-
-
?
polygalacturonan + H2O
?
-
-
-
-
?
potato pectin + H2O
? + acetate
-
-
-
-
?
racemic 1-phenylethyl acetate + H2O
(1R)-phenylethanol + (1S)-phenylethanol + acetate
-
-
-
-
?
sugar beet pectin + H2O
? + acetate
-
highest activity
-
-
?
tert-butyl acetate + H2O
2-methylpropane + acetate
-
-
-
?
tobacco hemicellulose + H2O
?
-
lowest activity
-
-
?
triacetin + H2O
?
-
EST1, pH 8, 20°C, 0.5% (w/v) Triton X-100, 0.125% (w/v) Arabic gum
titration assay
-
?
triacetine + H2O
?
-
-
-
-
?
tripropionin + H2O
?
-
-
-
-
?
xylobiose + vinyl acetate
? + vinyl alcohol
xylose tetraacetate + H2O
?
[(4S)-2,2-dimethyl-1,3-dioxolan-4-yl]methyl acetate + H2O
[(4R)-2,2-dimethyl-1,3-dioxolan-4-yl]methanol + acetate
-
fresh bacterial cells or purified enzyme (EST1 or EST2, 0.01 micromol p-nitrophenol/min), 20°C, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol
high enantioselectivity (enantiomeric ratio E: 20 for EST1, E: 15 for whole cells), thin-layer chromatography, enantiomeric composition of reactants controlled by gas chromatography, acetate detection spectrophotometrically through NADH absorbance at 340 nm
-
?
additional information
?
-
(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O
?
-
highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30°C to 60°C. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride
-
-
?
(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O
?
-
highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30°C to 60°C. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride
-
-
?
(acetyloxy)(phenyl)acetic acid + H2O
(S)-mandelic acid + (2R)-(acetyloxy)(phenyl)ethanoic acid
-
-
-
?
(acetyloxy)(phenyl)acetic acid + H2O
(S)-mandelic acid + (2R)-(acetyloxy)(phenyl)ethanoic acid
-
-
-
?
1-naphthol acetate + H2O
1-naphthol + acetate
-
-
-
-
?
1-naphthol acetate + H2O
1-naphthol + acetate
-
-
-
-
?
1-naphthol acetate + H2O
1-naphthol + acetate
-
-
-
-
?
1-naphthol acetate + H2O
1-naphthol + acetate
-
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
product determination by NMR and mass spectroscopy
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
highest activity (100%)
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
-
?
2-benzylcyclohex-1-en-1-yl acetate + H2O
2-benzylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product
-
-
?
2-benzylcyclohex-1-en-1-yl acetate + H2O
2-benzylcyclohexanone + acetate
-
isoenzyme Est II, 11% conversion of substrate with R-configuration of product
-
-
?
2-n-pentylcyclohex-1-en-1-yl acetate + H2O
2-n-pentylcyclohexanone + acetate
-
isoenzyme Est I, 20% conversion of substrate with R-configuration of product
-
-
?
2-n-pentylcyclohex-1-en-1-yl acetate + H2O
2-n-pentylcyclohexanone + acetate
-
isoenzyme Est II, 16% conversion of substrate with S-configuration of product
-
-
?
2-naphthol acetate + H2O
2-naphthol + acetate
-
-
-
-
?
2-naphthol acetate + H2O
2-naphthol + acetate
-
-
-
-
?
2-naphthyl acetate + H2O
2-naphthol + acetate
-
-
-
-
?
2-naphthyl acetate + H2O
2-naphthol + acetate
about 40% activity compared to 1-naphthyl acetate
-
-
?
2-naphthyl acetate + H2O
2-naphthol + acetate
-
-
-
-
?
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
2-t-butylcyclohex-1-en-1-yl acetate + H2O
2-t-butylcyclohexanone + acetate
-
isoenzyme Est I, 21% conversion of substrate with S-configuration of product
-
-
?
2-t-butylcyclohex-1-en-1-yl acetate + H2O
2-t-butylcyclohexanone + acetate
-
isoenzyme Est II, 20% conversion of substrate with R-configuration of product
-
-
?
3,6-diacetylmorphine + H2O
morphine + acetate
-
i.e. heroin, rapid spontaneous hydrolysis of the instable 3-acetyl group
-
?
3,6-diacetylmorphine + H2O
morphine + acetate
-
i.e. heroin, rapid spontaneous hydrolysis of the instable 3-acetyl group
-
?
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
3-O-methyl-D-glucopyranoside + vinyl acetate
6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol
-
-
-
-
?
3-O-methyl-D-glucopyranoside + vinyl acetate
6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
?
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
?
-
-
-
-
?
4-methylumbelliferyl acetate + H2O
?
-
-
-
-
?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
serine esterase activity
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
determination of specific activity
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
product determination by NMR and mass spectroscopy
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
highest activity
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
highest activity
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
highest activity
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
5 min, 30°C, pH 5.8, for kinetic analyses: 0.05-5 mM substrate in 100 mM sodium phosphate buffer
analysis by spectrophotometry at 410 nm
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
lowest activity
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
lowest activity
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
-
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
5 min, 30°C, pH 5.8
-
-
?
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
-
-
-
-
?
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
-
-
-
-
?
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
-
-
-
-
?
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O
?
-
-
-
-
?
6-acetylmorphine + H2O
morphine + acetate
-
substrate specificity
-
?
6-acetylmorphine + H2O
morphine + acetate
-
substrate specificity
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
?
acetylxylan + H2O
?
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
acetylxylan + H2O
?
-
-
-
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
-
-
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
-
-
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
EST1, non-denaturating PAGE, pH7.4
zymogram analyses using Fast Blue PR salt
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
EST1, non-denaturating PAGE, pH7.4
zymogram analyses using Fast Blue PR salt
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
15 min, 50°C, pH 6.5
-
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
-
-
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
-
-
-
?
astragaloside I + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
astragaloside I + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
astragaloside II + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
astragaloside II + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
beta-naphthyl acetate + H2O
beta-naphthol + acetate
-
-
-
?
beta-naphthyl acetate + H2O
beta-naphthol + acetate
-
sucrose density gradient cell fractions from Hep-G2 and RINm5F cells, pH 8
SDS-PAGE and in gel-zymography
-
?
cellobiose + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
cellobiose + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
cellulose acetate + H2O
cellulose + acetate
-
also in form of acetylated starch, water-soluble and water-insoluble substrate, specific for acetyl substituents at the C2- and C3-positions, C6-acetyl is not hydrolyzed
-
?
cellulose acetate + H2O
cellulose + acetate
-
-
-
-
?
cellulose acetate + H2O
cellulose + acetate
-
-
-
?
D-galactose + vinyl acetate
? + vinyl alcohol
-
isozyme CE2B shows 89% transacetylation conversion
-
-
?
D-galactose + vinyl acetate
? + vinyl alcohol
-
isozyme CE2B shows 22% transacetylation conversion, isozyme CE2A shows 48% transacetylation conversion
-
-
?
D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
D-glucose + vinyl acetate
6-O-acetyl-D-glucopyranose + vinyl alcohol
-
isozyme CE2B shows 86% transacetylation conversion
-
-
?
D-glucose + vinyl acetate
6-O-acetyl-D-glucopyranose + vinyl alcohol
-
isozyme CE2B shows 31% transacetylation conversion, isozyme CE2A shows 25% transacetylation conversion
-
-
?
D-glucose penta-acetate + H2O
?
-
-
-
-
?
D-glucose penta-acetate + H2O
?
-
-
-
?
D-mannose + vinyl acetate
6-O-acetyl-D-mannopyranose + vinyl alcohol
-
isozyme CE2B shows 71% transacetylation conversion
-
-
?
D-mannose + vinyl acetate
6-O-acetyl-D-mannopyranose + vinyl alcohol
-
isozyme CE2B shows 46% transacetylation conversion, isozyme CE2A shows 26% transacetylation conversion
-
-
?
D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
ethyl acetate + H2O
ethanol + acetate
-
-
-
-
?
ethyl acetate + H2O
ethanol + acetate
-
-
-
-
?
ethyl acetate + H2O
ethanol + acetate
-
25°C, 1 h, pH 7, 7 mM MgCl2, cell extracts
-
-
?
glucose pentaacetate + H2O
?
-
good substrate
-
?
glucose pentaacetate + H2O
?
-
-
-
-
?
glucose pentaacetate + H2O
?
-
-
-
-
?
glycerol triacetate + H2O
?
-
-
-
-
?
glycerol triacetate + H2O
?
-
-
-
-
?
isoastragaloside I + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
isoastragaloside I + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
isoastragaloside II + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
isoastragaloside II + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O
?
-
-
-
-
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O
?
-
-
-
-
?
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + H2O
?
-
-
-
-
?
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + H2O
?
-
-
-
-
?
methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + H2O
?
-
-
-
-
?
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + H2O
?
-
-
-
-
?
methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl 6-O-acetyl-beta-D-glucopyranoside + H2O
?
-
-
-
-
?
methyl 6-O-acetyl-beta-D-glucopyranoside + H2O
?
-
-
-
-
?
methyl acetate + H2O
methanol + acetate
-
-
-
-
?
methyl acetate + H2O
methanol + acetate
-
-
-
-
?
methyl alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
O-acetyl-1,4-beta-xylooligosaccharide + H2O
1,4-beta-xylooligosaccharide + acetic acid
-
-
-
ir
O-acetyl-1,4-beta-xylooligosaccharide + H2O
1,4-beta-xylooligosaccharide + acetic acid
-
-
-
-
ir
O-acetyl-4-O-methyl-D-glucurono-D-xylan + H2O
4-O-methyl-D-glucurono-D-xylan + acetic acid
-
-
-
-
?
O-acetyl-4-O-methyl-D-glucurono-D-xylan + H2O
4-O-methyl-D-glucurono-D-xylan + acetic acid
-
-
-
ir
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
Candida bororiensis
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
EST1, pH 7.4, 20°C, 0.5% (w/v) Triton X-100, 0.125% (w/v) Arabic gum
spectrophotometry, 400 nm
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
10 min, 50°C, pH 6.5
-
-
?
p-nitrophenyl hexanoate + H2O
p-nitrophenol + hexanoate
-
-
-
-
?
p-nitrophenyl hexanoate + H2O
p-nitrophenol + hexanoate
-
-
-
-
?
p-nitrophenyl laurate + H2O
p-nitrophenol + laurate
-
-
-
-
?
p-nitrophenyl laurate + H2O
p-nitrophenol + laurate
-
-
-
-
?
p-nitrophenyl propionate + H2O
p-nitrophenol + propionate
-
-
-
-
?
p-nitrophenyl propionate + H2O
p-nitrophenol + propionate
-
-
-
-
?
phenyl acetate + H2O
phenol + acetate
-
sucrose density gradient cell fractions from Hep-G2 and RINm5F cells, pH 8, 3 min, 1 mM CaCl2 or 5 mM EDTA
spectrophotometry, absorbance at 270 nm
-
?
phenyl acetate + H2O
phenol + acetate
-
-
-
?
phenyl acetate + H2O
phenol + acetate
-
-
-
?
tributyrin + H2O
?
-
-
-
?
tributyrin + H2O
?
-
EST1, pH 8, 20°C, 0.5% (w/v) Triton X-100, 0.125% (w/v) Arabic gum
titration assay
-
?
xanthan + H2O
?
the enzyme removes 57% of all acetyl groups present in xanthan after a 72 h incubation which corresponds to the removal of about 70% of all acetyl groups positioned on the inner mannose unit
-
-
?
xanthan + H2O
?
the enzyme removes 57% of all acetyl groups present in xanthan after a 72 h incubation which corresponds to the removal of about 70% of all acetyl groups positioned on the inner mannose unit
-
-
?
xylobiose + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
xylobiose + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
xylose tetraacetate + H2O
?
-
-
-
-
?
xylose tetraacetate + H2O
?
-
-
-
-
?
additional information
?
-
-
no transacetylation occurs with vinyl acetate and D-xylose, xylobiose, methyl 6-O-methyl-alpha-D-glucopyranoside, 6-O-methyl D-glucopyranose, and methyl beta-D-xylopyranoside
-
-
?
additional information
?
-
-
the enzyme shows a strong preference for deacetylation of the 6-position in aldohexoses. Xylose and xylooligosaccharides do not serve as acetyl group acceptors
-
-
?
additional information
?
-
-
enzyme has receptor-destroying enzyme function
-
?
additional information
?
-
-
substrate specificity and regioselectivity, deacetylation activity of the enzyme is not restricted to polymers with beta-1,4 glycosidic bonds, no activity with cellulose sulfate, the enzyme enhances endoglucanase, but is itself not influenced by endoglucanase
-
?
additional information
?
-
-
the enzyme, feruloyl esterase AoFae, behaves as a non-specific acetyl esterase rather than a feruloyl esterase, with a preference for 2-O-acetyl-beta-D-xylopyranoside, AoFae shows only a very weak activity on feruloyl esters
-
-
?
additional information
?
-
-
1-(4-fluorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(4-chlorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(4-methylphenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(3-fluorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(3,4-difluorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate are substrates of double mutant E188W/M193C, conversion with inversed enantioselectivity
-
-
?
additional information
?
-
-
1-phenyl-1-(trifluoromethyl)but-2-yn-1-yl acetate and 1-phenyl-1-(trifluoromethyl)prop-2-en-1-yl acetate are no substrates of neither wild-type nor any mutant
-
-
?
additional information
?
-
-
size of residue at position 188 (from Asp to Trp) determines enantioselectivity and -preference
-
-
?
additional information
?
-
-
no transacetylation occurs with vinyl acetate and D-xylose, xylobiose, methyl 6-O-methyl-alpha-D-glucopyranoside, 6-O-methyl D-glucopyranose, and methyl beta-D-xylopyranoside
-
-
?
additional information
?
-
-
the enzyme shows a strong preference for deacetylation of the 6-position in aldohexoses. Xylose and xylooligosaccharides do not serve as acetyl group acceptors
-
-
?
additional information
?
-
-
removal of acetyl group in 3-position of beta-lactams with 98% conversion and 91-93% product yield
-
-
?
additional information
?
-
-
the enzyme deacetylates cereal products like water soluble polysaccharides such as ragi, wheat, larch wood xylan, and gum karaya, the enzyme cleaves the acetyl groups substituted at O-2/O-3 of the xylan backbone of arabinoxylans and is known to modulate their functional properties
-
-
?
additional information
?
-
-
activated Sepharose CL6B-bound activity, pH 8
-
-
?
additional information
?
-
-
activity of EST1 only on short chain triglyceride substrates
-
-
?
additional information
?
-
-
EST2 shows lower activity and enantioselectivity towards (R,S)-1,2-O-isopropylidene glycol acetate as substrate than EST1
-
-
?
additional information
?
-
-
no activity of EST1 on p-nitrophenyl butyrate, p-nitrophenyl caproate, p-nitrophenyl caprylate, p-nitrophenyl laurate and p-nitrophenyl palmitate
-
-
?
additional information
?
-
-
no activity of EST1 on triacylglyceride esters tricaprylin and triolein
-
-
?
additional information
?
-
-
activity of EST1 only on short chain triglyceride substrates
-
-
?
additional information
?
-
-
EST2 shows lower activity and enantioselectivity towards (R,S)-1,2-O-isopropylidene glycol acetate as substrate than EST1
-
-
?
additional information
?
-
-
no activity of EST1 on p-nitrophenyl butyrate, p-nitrophenyl caproate, p-nitrophenyl caprylate, p-nitrophenyl laurate and p-nitrophenyl palmitate
-
-
?
additional information
?
-
-
no activity of EST1 on triacylglyceride esters tricaprylin and triolein
-
-
?
additional information
?
-
-
the enzyme does not attack the 3-O-acetyl group of xylopyranosyl residues carrying 4-O-methyl-D-glucuronic acid at position 2
-
-
?
additional information
?
-
-
the enzyme does not attack the 3-O-acetyl group of xylopyranosyl residues carrying 4-O-methyl-D-glucuronic acid at position 2
-
-
?
additional information
?
-
-
the enzyme has no activity toward 4-nitrophenyl valerate, 4-nitrophenyl caproate, 4-nitrophenyl palmitate, 4-nitrophenyl benzoate, 4-nitrophenyl phosphate, methyl benzoate, methyl 4-hydroxybenzoate, or methyl cyclohexane-1-carboxylate
-
-
?
additional information
?
-
no activity with 1-naphthyl phosphate, 4-methylumbelliferyl phosphate, linalyl acetate, alpha-terpinyl acetate, glyceryl trioleate, fish oil, or olive oil
-
-
?
additional information
?
-
-
no activity with 1-naphthyl phosphate, 4-methylumbelliferyl phosphate, linalyl acetate, alpha-terpinyl acetate, glyceryl trioleate, fish oil, or olive oil
-
-
?
additional information
?
-
-
no activity on p-nitrophenyl-beta-D-xylopyranoside and p-nitrophenyl-alpha-L-arabinofuranoside and no xylanolytic activity
-
-
?
additional information
?
-
-
no activity with 4-nitrophenyl octanoate. The enzyme does not exhibit de-esterification activity toward an acetylated xylooligosaccharide extracted from hot-water treated eucalyptus
-
-
?
additional information
?
-
-
the enzyme has no activity with acetylated xylans
-
-
?
additional information
?
-
-
no activity is detected on xylan or acetylated xylan or N-acetyl-D-glucosamine
-
-
?
additional information
?
-
new family of microbial esterases with hemicellulase activity for biodegradation of lignocellulose, complementary activity to acetylxylan esterase Axe1
-
-
?
additional information
?
-
-
acetylesterases generally prefer aliphatic substrates involving acetic acid
-
-
?
additional information
?
-
-
isoamyl acetate and ethyl acetate-synthesizing esterase activities in mutant strain 100fold less than respective acetate ester-hydrolysing esterase activities
-
-
?
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0.07
(R)-1,2-O-isopropylidene glycol acetate
-
EST1, after preparative electrophoresis
2.63
1-acetoxynaphthalene
-
pH 7.8, 45°C
1.54
1-naphthol acetate
-
-
0.06 - 0.97
2-benzylcyclohex-1-en-1-yl acetate
0.11
2-ethylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35°C
1.87
2-isopropylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35°C
0.09
2-methylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35°C
0.3 - 0.72
2-n-pentylcyclohex-1-en-1-yl acetate
0.54
2-n-propylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35°C
4.42
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C
3.6
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside
-
in 50 mM citrate-phosphate (pH 6) at 70°C
2.01 - 2.54
2-t-butylcyclohex-1-en-1-yl acetate
1.55
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C
4.2
3-O-acetyl-4-nitrophenyl beta-D-xylopyranoside
-
in 50 mM citrate-phosphate (pH 6) at 70°C
0.185 - 5.1
4-nitrophenyl acetate
0.057 - 7.1
4-nitrophenyl butyrate
0.245 - 0.355
4-nitrophenyl hexanoate
0.137
4-nitrophenyl propionate
-
in 50 mM citrate-phosphate (pH 6) at 70°C
44.6
4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside
-
pH 5.5, 45°C
3.28
4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside
-
pH 5.5, 45°C
9.44
4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside
-
pH 5.5, 45°C
6.78
4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside
-
pH 5.5, 45°C
16.4
4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside
-
pH 5.5, 45°C
0.75 - 2
4-nitrophenyl-butanoate
1.24 - 4.31
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
4
4-O-acetyl-4-nitrophenyl beta-D-xylopyranoside
-
in 50 mM citrate-phosphate (pH 6) at 70°C
0.5
6-acetylmorphine
-
recombinant enzyme, pH 8.0
2.7 - 7.8
Alpha-naphthyl acetate
9.1
Cellulose acetate
-
pH 5.0, 45°C
-
90
glyceryl triacetate
-
-
0.15
methylumbelliferone acetate
-
-
0.0067 - 385
p-nitrophenyl acetate
0.07
phenyl acetate
-
recombinant enzyme, pH 6.4
0.06
2-benzylcyclohex-1-en-1-yl acetate
-
isoenzyme Est II, pH 7.0, 35°C
0.97
2-benzylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35°C
0.3
2-n-pentylcyclohex-1-en-1-yl acetate
-
isoenzyme Est II, pH 7.0, 35°C
0.72
2-n-pentylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35°C
2.01
2-t-butylcyclohex-1-en-1-yl acetate
-
isoenzyme Est II, pH 7.0, 35°C
2.54
2-t-butylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35°C
0.185
4-nitrophenyl acetate
-
in 50 mM citrate-phosphate (pH 6) at 70°C
0.23
4-nitrophenyl acetate
30°C, pH 5.8
0.23
4-nitrophenyl acetate
purified recombinant enzyme, 30°C, pH 5.8
0.285
4-nitrophenyl acetate
-
at pH 8.0 and 20°C
0.84
4-nitrophenyl acetate
at pH 7.0 and 39°C
1.1
4-nitrophenyl acetate
-
wild type enzyme, at pH 5.5 and 50°C
1.4
4-nitrophenyl acetate
-
mutant enzyme D179A, at pH 5.5 and 50°C
1.8
4-nitrophenyl acetate
-
mutant enzyme H182A, at pH 5.5 and 50°C
3.76
4-nitrophenyl acetate
-
at pH 8.0 and 35°C
4.7
4-nitrophenyl acetate
-
mutant enzyme C16A, at pH 5.5 and 50°C
5.1
4-nitrophenyl acetate
-
mutant enzyme S10A, at pH 5.5 and 50°C
0.057
4-nitrophenyl butyrate
-
mutant enzyme L209F, in 20 mM phosphate buffer, at pH 7.1 and 30°C
0.069
4-nitrophenyl butyrate
-
mutant enzyme L97F/L209F, in 20 mM phosphate buffer, at pH 7.1 and 30°C
0.13
4-nitrophenyl butyrate
mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C
0.14
4-nitrophenyl butyrate
mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C
0.16
4-nitrophenyl butyrate
wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C
0.17
4-nitrophenyl butyrate
-
wild type enzyme, in 20 mM phosphate buffer, at pH 7.1 and 30°C
0.18
4-nitrophenyl butyrate
mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C
0.19
4-nitrophenyl butyrate
mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C
0.26
4-nitrophenyl butyrate
-
mutant enzyme L97F, in 20 mM phosphate buffer, at pH 7.1 and 30°C
7.1
4-nitrophenyl butyrate
-
wild type enzyme, at pH 5.5 and 50°C
0.245
4-nitrophenyl hexanoate
-
pH 7.1, 65°C, recombinant wild-type enzyme
0.355
4-nitrophenyl hexanoate
-
pH 7.1, 65°C, recombinant mutant T74A
0.75
4-nitrophenyl-butanoate
-
pH 7.1, 25°C, recombinant wild-type enzyme
1.5
4-nitrophenyl-butanoate
-
pH 7.1, 65°C, recombinant wild-type enzyme
2
4-nitrophenyl-butanoate
-
pH 7.1, 65°C, recombinant mutant T74A
1.24
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C
2.46
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C
4.31
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C
2.7
Alpha-naphthyl acetate
-
-
7.8
Alpha-naphthyl acetate
-
pH 6.5
0.0067
p-nitrophenyl acetate
-
-
0.023
p-nitrophenyl acetate
-
-
0.43
p-nitrophenyl acetate
-
pH 6.5
0.9
p-nitrophenyl acetate
-
-
32.5
p-nitrophenyl acetate
-
pH 7.0, 25°C, presence of benzethonium chloride
385
p-nitrophenyl acetate
-
pH 7.0, 25°C, absence of benzethonium chloride
0.015
tributyrin
mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C
0.018
tributyrin
mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C
0.047
tributyrin
mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C
0.052
tributyrin
wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C
0.073
tributyrin
mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C
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20 - 33
2-benzylcyclohex-1-en-1-yl acetate
63
2-ethylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35°C
14
2-isopropylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35°C
77
2-methylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35°C
26 - 42
2-n-pentylcyclohex-1-en-1-yl acetate
79
2-n-propylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35°C
41.6
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C
76.1
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside
-
in 50 mM citrate-phosphate (pH 6) at 70°C
7 - 17
2-t-butylcyclohex-1-en-1-yl acetate
2101
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C
70.1
3-O-acetyl-4-nitrophenyl beta-D-xylopyranoside
-
in 50 mM citrate-phosphate (pH 6) at 70°C
0.82 - 57.5
4-nitrophenyl acetate
29 - 340
4-nitrophenyl butyrate
34.5 - 48.8
4-nitrophenyl hexanoate
41.3
4-nitrophenyl propionate
-
in 50 mM citrate-phosphate (pH 6) at 70°C
0.15
4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside
-
pH 5.5, 45°C
3.23
4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside
-
pH 5.5, 45°C
1.9
4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside
-
pH 5.5, 45°C
0.91
4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside
-
pH 5.5, 45°C
1.77
4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside
-
pH 5.5, 45°C
21 - 155
4-nitrophenyl-butanoate
241.6 - 3210
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
78.6
4-O-acetyl-4-nitrophenyl beta-D-xylopyranoside
-
in 50 mM citrate-phosphate (pH 6) at 70°C
12.6
6-acetylmorphine
-
recombinant enzyme, pH 8.0
3
phenyl acetate
-
recombinant enzyme, pH 6.4
additional information
4-nitrophenyl-beta-D-xylopyranoside monoacetate
initial rate ratio between 2-, 3-, and 4-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside is 1:19:17.7
20
2-benzylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35°C
33
2-benzylcyclohex-1-en-1-yl acetate
-
isoenzyme Est II, pH 7.0, 35°C
26
2-n-pentylcyclohex-1-en-1-yl acetate
-
isoenzyme Est II, pH 7.0, 35°C
42
2-n-pentylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35°C
7
2-t-butylcyclohex-1-en-1-yl acetate
-
isoenzyme Est I, pH 7.0, 35°C
17
2-t-butylcyclohex-1-en-1-yl acetate
-
isoenzyme Est II, pH 7.0, 35°C
0.82
4-nitrophenyl acetate
at pH 7.0 and 39°C
5.5
4-nitrophenyl acetate
-
at pH 8.0 and 20°C
21.8
4-nitrophenyl acetate
-
mutant enzyme S10A, at pH 5.5 and 50°C
26.2
4-nitrophenyl acetate
-
mutant enzyme C16A, at pH 5.5 and 50°C
36.3
4-nitrophenyl acetate
-
mutant enzyme D179A, at pH 5.5 and 50°C
38.8
4-nitrophenyl acetate
-
mutant enzyme H182A, at pH 5.5 and 50°C
49.2
4-nitrophenyl acetate
-
wild type enzyme, at pH 5.5 and 50°C
57.5
4-nitrophenyl acetate
-
in 50 mM citrate-phosphate (pH 6) at 70°C
29
4-nitrophenyl butyrate
mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C
29
4-nitrophenyl butyrate
wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C
29
4-nitrophenyl butyrate
-
wild type enzyme, in 20 mM phosphate buffer, at pH 7.1 and 30°C
29.8
4-nitrophenyl butyrate
-
wild type enzyme, at pH 5.5 and 50°C
37
4-nitrophenyl butyrate
-
mutant enzyme L209F, in 20 mM phosphate buffer, at pH 7.1 and 30°C
78
4-nitrophenyl butyrate
mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C
86
4-nitrophenyl butyrate
mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C
113
4-nitrophenyl butyrate
mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C
240
4-nitrophenyl butyrate
-
mutant enzyme L97F, in 20 mM phosphate buffer, at pH 7.1 and 30°C
340
4-nitrophenyl butyrate
-
mutant enzyme L97F/L209F, in 20 mM phosphate buffer, at pH 7.1 and 30°C
34.5
4-nitrophenyl hexanoate
-
pH 7.1, 65°C, recombinant wild-type enzyme
48.8
4-nitrophenyl hexanoate
-
pH 7.1, 65°C, recombinant mutant T74A
21
4-nitrophenyl-butanoate
-
pH 7.1, 25°C, recombinant wild-type enzyme
145
4-nitrophenyl-butanoate
-
pH 7.1, 65°C, recombinant wild-type enzyme
155
4-nitrophenyl-butanoate
-
pH 7.1, 65°C, recombinant mutant T74A
241.6
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C
1331
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C
3210
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside
-
isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C
6.2
tributyrin
wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C
6.9
tributyrin
mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C
9.3
tributyrin
mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C
17.4
tributyrin
mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C
17.5
tributyrin
mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C
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0.000109
4-nitrophenyl butyrate, purified recombinant enzyme
0.000213
4-nitrophenyl formate, purified recombinant enzyme
0.000225
-
mutant strain, ethyl acetate-hydrolysing esterase (EAHase) activity
0.000236
-
mutant strain, isoamyl acetate-hydrolysing esterase (IAHase) activity
0.000342
-
wild-type strain, ethyl acetate-hydrolysing esterase (EAHase) activity
0.000347
-
wild-type strain, isoamyl acetate-hydrolysing esterase (IAHase) activity
0.00507
-
with 4-nitrophenyl butyrate as substrate, at pH 6.0 and 35°C
0.021
Candida bororiensis
-
-
0.027
-
purified native enzyme, substrate ragi
0.061
-
purified native enzyme, substrate larch wood xylan
0.082
-
substrate p-nitrophenyl laurate, pH 7.0, 25°C
0.251
-
substrate p-nitrophenyl acetate, pH 7.0, 25°C
0.282
-
purified native enzyme, substrate gum karaya
0.297
-
substrate p-nitrophenyl decanoate, pH 7.0, 25°C
0.352
-
substrate p-nitrophenyl hexanoate, pH 7.0, 25°C
0.369
-
substrate p-nitrophenyl propionate, pH 7.0, 25°C
0.474
-
substrate p-nitrophenyl butyrate, pH 7.0, 25°C
0.52
-
crude enzyme, at pH 8.0 and 35°C
0.85
recombinant enzyme, after CM Sepharose FF column
1.12
recombinant enzyme, after DEAE FF column, 4-nitrophenyl acetate
1.21
4-nitrophenyl acetate, purified recombinant enzyme
10.3
-
with 4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35°C
1027
-
alpha-naphthyl acetate, pH 6.5
104.85
-
with 4-nitrophenyl acetate as substrate, at pH 6.0 and 35°C
13.3
-
with 4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35°C
2.37
-
with 4-nitrophenyl propionate as substrate, at pH 6.0 and 35°C
20.5
4-nitrophenyl acetate, maximum velocity, purified recombinant enzyme, 30°C, pH 5.8
221.9
-
after 423.39fold purification, at pH 8.0 and 35°C
250
-
pH 7.1, 65°C, purified recombinant wild-type enzyme
270
-
pH 7.1, 65°C, purified recombinant mutant T74A
3.38
-
purified native enzyme, substrate alpha-naphthyl acetate
324
-
(R,S)-1,2-O-isopropylidene glycol acetate, EST1, after preparative electrophoresis
328
-
isozyme CE2B, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C
333
-
wild-type, using 4-nitrophenyl acetate as substrate
35
-
isozyme CE2, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C
395
-
isozyme CE2B, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C
4
-
isozyme CE2B, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C
4.44
-
purified native enzyme, substrate wheat
46.5
-
mutant E188W/M193C, using 4-nitrophenyl acetate as substrate
5.39
-
purified native enzyme, substrate 4-nitrophenyl acetate
5.64
-
with 4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35°C
60
-
isozyme CE2C, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C
71
-
p-nitrophenyl acetate, pH 6.5
8
-
isozyme CE2, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C
9.63
-
p-nitrophenyl acetate, after hydroxyapatite chromatography, pH 6.5
0.21
4-nitrophenyl acetate, over-expression culture supernatant
0.21
recombinant enzyme, culture supernatant
183
-
isozyme CE2, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C
183
-
isozyme CE2, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C
2
-
isozyme CE2, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C
2
-
isozyme CE2C, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C
3
-
isozyme CE2C, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C
3
-
isozyme CE2C, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C
5
-
-
5
-
isozyme CE2B, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C
additional information
-
substrate specificity
additional information
-
-
additional information
-
-
additional information
-
isoforms with pI-values 4.2 and 5.0 are the most active ones in all samples
additional information
-
(R)-1,2-O-isopropylidene glycol acetate, EST1, 1.0 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
(R,S)-1,2-O-isopropylidene glycol acetate as substrate for EST1, 20 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
(R,S)-1,2-O-isopropylidene glycol butyrate as substrate for EST1, 4.6 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
(R,S)-1,2-O-isopropylidene glycol caproate as substrate for EST1, 0.7 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
(S)-1,2-O-isopropylidene glycol acetate, EST1, 1.2 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
triacetin as substrate for EST1, 4.06 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
tributyrin as substrate for EST1, 2.8 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
extremly low activities against 4-nitrophenyl formate, butyrate, or canoate
additional information
-
mutant strain, 61% lower ethyl acetate-hydrolysing esterase (EAHase) activity than in wild-type strain
additional information
-
mutant strain, 68% lower isoamyl acetate-hydrolysing esterase (IAHase) activity than in wild-type strain
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Egana, L.; Gutierrez, R.; Caputo, V.; Peirano, A.; Steiner, J.; Eyzaguirre, J.
Purification and characterization of two acetyl xylan esterases from Penicillium purpurogenum
Biotechnol. Appl. Biochem.
24
33-39
1996
Talaromyces purpureogenus
brenda
Kormelink, F.J.M.; Lefebvre, B.; Strozyk, F.; Voragen, A.G.J.
Purification and characterization of an acetyl xylan esterase from Aspergillus niger
J. Biotechnol.
27
267-282
1993
Aspergillus niger
-
brenda
Degrassi, G.; Okeke, B.C.; Bruschi, C.V.; Venturi, V.
Purification and characterization of an acetyl xylan esterase from Bacillus pumilus
Appl. Environ. Microbiol.
64
789-792
1998
Bacillus pumilus
brenda
McDermid, K.P.; Forsberg, C.W.; MacKenzie, C.R.
Purification and properties of an acetylxylan esterase from Fibrobacter succinogenes S85
Appl. Environ. Microbiol.
56
3805-3810
1990
Fibrobacter succinogenes, Fibrobacter succinogenes S85
brenda
Lee, H.; To, R.J.B.; Latta, R.K.; Biely, P.; Schneider, H.
Some properties of extracellular acetylxylan esterase produced by the yeast Rhodotorula mucilaginosa
Appl. Environ. Microbiol.
53
2831-2834
1987
Rhodotorula mucilaginosa
brenda
Hakulinen, N.; Tenkanen, M.; Rouvinen, J.
Crystallization and preliminary X-ray diffraction studies of the catalytic core of acetyl xylan esterase from Trichoderma reesei
Acta Crystallogr. Sect. D
54
430-432
1998
Trichoderma reesei
brenda
Biely, P.; MacKenzie, C.R.; Schneider, H.
Acetylxylan esterase of Schizophyllum commune
Methods Enzymol.
160
700-707
1988
Schizophyllum commune
-
brenda
Pullan, L.M.; Noltmann, E.A.
Purification and properties of pig muscle carbonic anhydrase III
Biochim. Biophys. Acta
839
147-154
1985
Sus scrofa
brenda
Johnson, K.G.; Fontana, J.D.; MacKenzie, C.R.
Measurement of acetylxylan esterase in Streptomyces
Methods Enzymol.
160
551-560
1988
Streptomyces olivochromogenes
-
brenda
Matern, U.
Acylhydrolases from parsley (Petroselinum hortense). Relative distribution and properties of four esterases hydrolyzing malonic acid hemiesters of flavonoid glucosides
Arch. Biochem. Biophys.
224
261-271
1983
Petroselinum crispum
brenda
Poutanen, K.; Sundberg, M.
An acetyl esterase of Trichoderma reesei and its role in the hydrolysis of acetyl xylans
Appl. Microbiol. Biotechnol.
28
419-424
1988
Trichoderma reesei
-
brenda
Arav, R.; Rimon, S.
Purification and characterization of acetyl esterase from bull testes
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