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2',3'-dehydro-3'-deoxyTDP + ATP
2',3'-dehydro-3'-deoxyTTP + ADP
-
-
-
?
3'-fluoro-2',3'-dideoxyUDP + ATP
3'-fluoro-2',3'-dideoxyUTP + ADP
better than 2,3-dideoxyUDP, but much poorer than 2-deoxyUDP
-
-
?
3'-fluoro-3'-deoxyTDP + ATP
3'-fluoro-3'-deoxyTTP + ADP
-
-
-
?
ADP + dADP
AMP + dATP
-
-
-
r
ADP + dCDP
AMP + dCTP
low activity
-
-
r
ADP + dCTP
ATP + dCDP
-
-
-
-
?
ADP + dGDP
AMP + dGTP
-
-
-
r
ADP + dTTP
ATP + dTDP
-
-
-
-
?
ADP + dUTP
ATP + dUDP
-
-
-
-
?
ADP + ITP
ATP + IDP
-
-
-
?
ATP + 8-bromo-IDP
ADP + ?
ATP + 8-bromoinosine 5'-diphosphate
ADP + 8-bromoinosine 5'-triphosphate
ATP + adenosine 5'-methylene diphosphonate
ADP + ?
-
-
-
?
ATP + nucleoside diphosphate
ADP + nucleoside triphosphate
ATP + tubulin-GDP
ADP + tubulin-GTP
-
-
-
?
ATP + XDP
ADP + XTP
-
reaction at 37% the rate of UDP
-
-
?
CTP + ADP
CDP + ATP
-
-
-
?
dATP + CDP
dADP + CTP
-
-
-
r
dATP + GDP
dADP + GTP
-
-
-
?
dATP + IDP
dADP + ITP
-
-
-
?
dATP + UDP
dADP + UTP
-
-
-
?
dGDP + ATP
dGTP + ADP
-
-
-
-
?
gamma-S-ATP + GDP
ADP + gamma-S-GTP
gamma-S-GTP + GDP
GDP + gamma-S-GTP
GDP + ATP
GTP + ADP
-
-
-
?
GTP + dGDP
GDP + dGTP
-
-
-
-
?
GTP + dTDP
GDP + dTTP
-
-
-
-
?
GTP + nucleoside diphosphate
GDP + nucleoside triphosphate
GTP-bound Ras protein + NDP
GDP-bound Ras protein + NTP
-
in vitro, inactivation of mutant oncogenic Ras proteins
-
-
?
ITP + UDP
IDP + UTP
-
-
-
-
?
NTP + ADP
NDP + ATP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
TTP + ADP
TDP + ATP
-
-
-
?
tubulin-GDP-complex + ATP
tubulin-GTP-complex + ADP
-
-
-
-
?
UTP + ADP
UDP + ATP
-
-
-
?
UTP + UDP
UDP + UTP
-
reaction at 31% the rate of ATP
-
?
additional information
?
-
ADP + ATP
ATP + ADP
-
-
-
?
ADP + ATP
ATP + ADP
-
-
-
?
ADP + CTP
ATP + CDP
-
-
-
-
?
ADP + CTP
ATP + CDP
-
-
-
?
ADP + CTP
ATP + CDP
-
-
-
?
ADP + CTP
ATP + CDP
-
-
-
-
?
ADP + CTP
ATP + CDP
-
-
-
?
ADP + dGTP
ATP + dGDP
-
-
-
?
ADP + dGTP
ATP + dGDP
-
-
-
?
ADP + GTP
ATP + GDP
-
-
-
-
?
ADP + GTP
ATP + GDP
-
-
-
?
ADP + GTP
ATP + GDP
-
-
-
?
ADP + GTP
ATP + GDP
-
-
-
-
?
ADP + GTP
ATP + GDP
-
-
-
?
ADP + GTP
ATP + GDP
-
-
-
-
?
ADP + GTP
ATP + GDP
-
-
-
?
ADP + TTP
ATP + TDP
-
-
-
?
ADP + TTP
ATP + TDP
-
-
-
-
?
ADP + UTP
ATP + UDP
-
-
-
-
?
ADP + UTP
ATP + UDP
-
-
-
?
ADP + UTP
ATP + UDP
-
-
-
?
ATP + 8-bromo-IDP
ADP + ?
-
-
-
?
ATP + 8-bromo-IDP
ADP + ?
-
-
-
?
ATP + 8-bromo-IDP
ADP + ?
-
-
-
?
ATP + 8-bromoinosine 5'-diphosphate
ADP + 8-bromoinosine 5'-triphosphate
-
-
-
?
ATP + 8-bromoinosine 5'-diphosphate
ADP + 8-bromoinosine 5'-triphosphate
-
-
-
?
ATP + 8-bromoinosine 5'-diphosphate
ADP + 8-bromoinosine 5'-triphosphate
-
-
-
?
ATP + 8-bromoinosine 5'-diphosphate
ADP + 8-bromoinosine 5'-triphosphate
-
-
-
?
ATP + ADP
ADP + ATP
-
best substrate
-
?
ATP + ADP
ADP + ATP
-
-
-
r
ATP + ADP
ADP + ATP
-
-
-
?
ATP + ADP
ADP + ATP
-
-
-
-
?
ATP + ADP
ADP + ATP
-
less effective dinucleotide substrate
-
?
ATP + ADP
ADP + ATP
-
-
-
?
ATP + ADP
ADP + ATP
-
reaction at 94% the rate of UDP
-
?
ATP + CDP
ADP + CTP
-
-
-
-
?
ATP + CDP
ADP + CTP
-
-
-
?
ATP + CDP
ADP + CTP
-
-
-
-
?
ATP + CDP
ADP + CTP
-
reverse reaction at 21% the rate of ATP plus ADP
-
r
ATP + CDP
ADP + CTP
-
-
-
-
?
ATP + CDP
ADP + CTP
-
-
-
-
r
ATP + CDP
ADP + CTP
-
-
-
-
r
ATP + CDP
ADP + CTP
-
-
-
-
r
ATP + CDP
ADP + CTP
-
-
-
-
r
ATP + CDP
ADP + CTP
-
-
-
-
r
ATP + CDP
ADP + CTP
-
-
-
-
r
ATP + CDP
ADP + CTP
-
-
-
-
?
ATP + CDP
ADP + CTP
-
outer mitochondrial membrane-bound NDPK activity is involved in functional coupling to oxidative phosphorylation
-
-
?
ATP + CDP
ADP + CTP
-
highly effective dinucleotide substrate
-
-
?
ATP + CDP
ADP + CTP
-
-
-
r
ATP + CDP
ADP + CTP
-
reaction at 92% the rate of UDP
-
-
?
ATP + CDP
ADP + CTP
-
-
-
-
?
ATP + CDP
ADP + CTP
-
-
-
-
?
ATP + CDP
ADP + CTP
-
-
-
-
?
ATP + dADP
ADP + dATP
-
-
-
?
ATP + dADP
ADP + dATP
-
less effective dinucleotide substrate
-
-
?
ATP + dADP
ADP + dATP
-
-
-
r
ATP + dADP
ADP + dATP
-
reaction at 59% the rate of UDP
-
-
?
ATP + dCDP
ADP + dCTP
-
-
-
-
?
ATP + dCDP
ADP + dCTP
-
-
-
?
ATP + dCDP
ADP + dCTP
-
-
-
-
?
ATP + dCDP
ADP + dCTP
-
-
-
r
ATP + dCDP
ADP + dCTP
-
-
-
?
ATP + dCDP
ADP + dCTP
-
-
-
-
r
ATP + dCDP
ADP + dCTP
-
less effective dinucleotide substrate
-
-
?
ATP + dCDP
ADP + dCTP
-
-
-
r
ATP + dCDP
ADP + dCTP
-
reaction at 61% the rate of UDP
-
-
?
ATP + dGDP
ADP + dGTP
-
-
-
?
ATP + dGDP
ADP + dGTP
-
-
-
r
ATP + dGDP
ADP + dGTP
dGDP is preferred over dTDP as an acceptor
-
-
?
ATP + dGDP
ADP + dGTP
-
-
-
-
r
ATP + dGDP
ADP + dGTP
-
less effective dinucleotide substrate
-
?
ATP + dGDP
ADP + dGTP
-
-
-
r
ATP + dGDP
ADP + dGTP
-
reaction at 55% the rate of UDP
-
?
ATP + dNDP
ADP + dNTP
contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates
-
-
?
ATP + dNDP
ADP + dNTP
contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates
-
-
?
ATP + dNDP
ADP + dNTP
contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates
-
-
?
ATP + dNDP
ADP + dNTP
contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates
-
-
?
ATP + dNDP
ADP + dNTP
contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates
-
-
?
ATP + dTDP
ADP + dTTP
-
-
-
-
?
ATP + dTDP
ADP + dTTP
-
-
-
?
ATP + dTDP
ADP + dTTP
-
in decreasing order of activity: UDP, dUDP, dCDP, GDP, dGDP, CDP
-
-
?
ATP + dTDP
ADP + dTTP
-
GTP can fully replace ATP
-
-
?
ATP + dTDP
ADP + dTTP
-
best substrate: dTDP
-
-
?
ATP + dTDP
ADP + dTTP
-
in decreasing order of activity: dGDP, IDP, UDP, dUDP, CDP, GDP, dCDP
-
?
ATP + dTDP
ADP + dTTP
-
best substrate: dTDP
-
?
ATP + dTDP
ADP + dTTP
-
-
-
-
?
ATP + dTDP
ADP + dTTP
-
-
-
?
ATP + dTDP
ADP + dTTP
-
-
-
r
ATP + dTDP
ADP + dTTP
-
-
-
?
ATP + dTDP
ADP + dTTP
-
-
-
?
ATP + dTDP
ADP + dTTP
-
-
-
-
?
ATP + dTDP
ADP + dTTP
-
-
-
-
?
ATP + dTDP
ADP + dTTP
-
-
-
-
r
ATP + dTDP
ADP + dTTP
-
highly effective dinucleotide substrate
-
?
ATP + dTDP
ADP + dTTP
-
reaction at 27% the rate of UDP
-
-
?
ATP + dTDP
ADP + dTTP
-
-
-
-
?
ATP + dTDP
ADP + dTTP
-
-
-
?
ATP + dTDP
ADP + dTTP
-
-
-
?
ATP + dTDP
ADP + dTTP
-
-
-
r
ATP + dTDP
ADP + dTTP
-
-
-
-
?
ATP + dUDP
?
-
highly effective dinucleotide substrate
-
-
?
ATP + dUDP
?
-
reaction at 39% the rate of UDP
-
-
?
ATP + dUDP
ADP + dUTP
-
-
-
-
?
ATP + dUDP
ADP + dUTP
-
-
-
?
ATP + GDP
ADP + GTP
-
-
-
-
?
ATP + GDP
ADP + GTP
-
-
-
?
ATP + GDP
ADP + GTP
-
-
-
r
ATP + GDP
ADP + GTP
-
-
-
-
?
ATP + GDP
ADP + GTP
assay by spontaneous release of GDP from G proteins. This GDP serves as a substrate for phosphotransfer by the NDPK, and the formed GTP then binds back to the G protein. NDPK is able to transfer phosphate also onto denatured proteins and thus also on the covalently linked GDP
-
-
r
ATP + GDP
ADP + GTP
-
-
-
-
r
ATP + GDP
ADP + GTP
-
-
-
-
r
ATP + GDP
ADP + GTP
-
-
-
-
?
ATP + GDP
ADP + GTP
-
-
-
?
ATP + GDP
ADP + GTP
-
-
-
?
ATP + GDP
ADP + GTP
-
-
-
-
r
ATP + GDP
ADP + GTP
-
-
-
-
r
ATP + GDP
ADP + GTP
-
-
-
-
r
ATP + GDP
ADP + GTP
-
-
-
-
?
ATP + GDP
ADP + GTP
-
-
-
-
?
ATP + GDP
ADP + GTP
-
-
-
-
r
ATP + GDP
ADP + GTP
-
-
-
?
ATP + GDP
ADP + GTP
-
-
-
-
?
ATP + GDP
ADP + GTP
-
an essential function of NDP kinase in ROS is phosphorylation of GDP to GTP, which is crucial for providing the functional cycle of transducin independently of its activation
-
-
?
ATP + GDP
ADP + GTP
-
assay by spontaneous release of GDP from G proteins. This GDP serves as a substrate for phosphotransfer by the NDPK, and the formed GTP then binds back to the G protein. NDPK is able to transfer phosphate also onto denatured proteins and thus also on the covalently linked GDP
-
-
r
ATP + GDP
ADP + GTP
-
-
-
-
?
ATP + GDP
ADP + GTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
?
ATP + NDP
ADP + NTP
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
-
?
ATP + NDP
ADP + NTP
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates, enzyme is able to specifically interact with proteins encoded by the bacteriophage T4
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
?
ATP + NDP
ADP + NTP
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates, NM23 metastasis suppressor involved in inactivation of oncogenic forms of Ras proteins
-
-
?
ATP + NDP
ADP + NTP
MgATP promotes correct folding and association
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
?
ATP + NDP
ADP + NTP
contributes to the maintenance of the cellular pools of all nucleoside triphosphates
-
-
?
ATP + NDP
ADP + NTP
contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ
-
-
?
ATP + NDP
ADP + NTP
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
-
?
ATP + NDP
ADP + NTP
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages
-
-
?
ATP + NDP
ADP + NTP
-
-
-
?
ATP + NDP
ADP + NTP
contributes to the maintenance of the cellular pools of all nucleoside triphosphates
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates, extracellular enzyme contributes to ATP-induced cell death of macrophages
-
-
?
ATP + NDP
ADP + NTP
-
-
-
?
ATP + NDP
ADP + NTP
contributes to the maintenance of the cellular pools of all nucleoside triphosphates, excreted enzyme enters the nucleus of HeLa and COS-1 cells and nicks chromosomal DNA in situ
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
r
ATP + NDP
ADP + NTP
-
-
-
-
r
ATP + NDP
ADP + NTP
-
-
-
?
ATP + NDP
ADP + NTP
contributes to the maintenance of the cellular pools of all nucleoside triphosphates, involved in coleoptile elongation and anaerobic stress response
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
r
ATP + NDP
ADP + NTP
-
-
-
?
ATP + NDP
ADP + NTP
contributes to the maintenance of the cellular pools of all nucleosides triphosphates, broad substrate specificity, in plants involved in different signaling pathways
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
-
?
ATP + NDP
ADP + NTP
-
-
-
-
?
ATP + NDP
ADP + NTP
-
contributes to the maintenance of the cellular pools of all nucleosides triphosphates
-
-
?
ATP + NDP
ADP + NTP
-
-
-
?
ATP + nucleoside diphosphate
ADP + nucleoside triphosphate
-
-
-
-
r
ATP + nucleoside diphosphate
ADP + nucleoside triphosphate
-
analysis of ATP binding by the enzyme at different concentrations and pH values, overview
-
-
r
ATP + nucleoside diphosphate
ADP + nucleoside triphosphate
-
-
-
r
ATP + nucleoside diphosphate
ADP + nucleoside triphosphate
this reaction is a reversible phosphate transfer to both ribonucleoside and deoxyribonucleoside diphosphates using NTP as a phosphate donor to provide adequate nucleosides for RNA or DNA synthesis
-
-
r
ATP + nucleoside diphosphate
ADP + nucleoside triphosphate
-
-
-
?
ATP + TDP
ADP + TTP
-
-
-
?
ATP + TDP
ADP + TTP
-
-
-
?
ATP + TDP
ADP + TTP
-
-
-
r
ATP + TDP
ADP + TTP
-
-
-
-
?
ATP + TDP
ADP + TTP
-
-
-
r
ATP + TDP
ADP + TTP
the enzyme activity depends largely upon the membrane-bound state
-
-
r
ATP + TDP
ADP + TTP
-
-
-
-
r
ATP + TDP
ADP + TTP
-
-
-
-
?
ATP + TDP
ADP + TTP
-
-
-
-
r
ATP + TDP
ADP + TTP
-
-
-
-
r
ATP + TDP
ADP + TTP
-
the enzyme activity depends largely upon the membrane-bound state
-
-
r
ATP + TDP
ADP + TTP
-
-
-
?
ATP + UDP
ADP + UTP
-
-
-
-
?
ATP + UDP
ADP + UTP
-
-
-
?
ATP + UDP
ADP + UTP
-
-
-
r
ATP + UDP
ADP + UTP
NDPK regulates a variety of eukaryotic cellular activities including cell proliferation, development, and differentiation
-
-
r
ATP + UDP
ADP + UTP
-
reverse reaction at 32% the rate of the reaction ATP plus ADP
-
r
ATP + UDP
ADP + UTP
-
-
-
-
r
ATP + UDP
ADP + UTP
-
-
-
-
r
ATP + UDP
ADP + UTP
-
-
-
-
r
ATP + UDP
ADP + UTP
-
-
-
-
r
ATP + UDP
ADP + UTP
-
-
-
-
?
ATP + UDP
ADP + UTP
-
highly effective dinucleotide substrate
-
-
?
ATP + UDP
ADP + UTP
-
-
-
?
ATP + UDP
ADP + UTP
-
best dinucleotide substrate
-
r
ATP + UDP
ADP + UTP
-
-
-
?
ATP + UDP
ADP + UTP
-
-
-
-
?
CTP + CDP
CDP + CTP
-
reaction at 15% the rate of ATP
-
?
CTP + CDP
CDP + CTP
-
-
-
?
dATP + ADP
dADP + ATP
at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C
-
-
?
dATP + ADP
dADP + ATP
at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C
-
-
?
dCTP + ADP
dCDP + ATP
at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C
-
-
?
dCTP + ADP
dCDP + ATP
at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C
-
-
?
dGTP + ADP
dGDP + ATP
-
-
-
-
?
dGTP + ADP
dGDP + ATP
the wild-type enzyme is not active when using dGTP as the phosphate donor. The Pyrobaculum aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66100 and 156165. Deletion mutants of the NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor
-
-
?
dGTP + ADP
dGDP + ATP
the wild-type enzyme is not active when using dGTP as the phosphate donor. The Pyrobaculum aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66100 and 156165. Deletion mutants of the NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor
-
-
?
dTTP + ADP
dTDP + ATP
at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C
-
-
?
dTTP + ADP
dTDP + ATP
at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C
-
-
?
gamma-S-ATP + GDP
ADP + gamma-S-GTP
-
poor substrate
-
-
?
gamma-S-ATP + GDP
ADP + gamma-S-GTP
-
poor substrate
-
-
?
gamma-S-ATP + GDP
ADP + gamma-S-GTP
-
poor substrate
-
-
?
gamma-S-GTP + GDP
GDP + gamma-S-GTP
-
poor substrate
-
-
?
gamma-S-GTP + GDP
GDP + gamma-S-GTP
-
poor substrate
-
-
?
gamma-S-GTP + GDP
GDP + gamma-S-GTP
-
poor substrate
-
-
?
GTP + ADP
GDP + ATP
-
-
-
-
?
GTP + ADP
GDP + ATP
-
-
-
-
?
GTP + ADP
GDP + ATP
-
reaction at 12% the rate of ATP
-
-
?
GTP + ADP
GDP + ATP
-
dADP can replace ADP
-
r
GTP + ADP
GDP + ATP
-
-
-
?
GTP + ADP
GDP + ATP
-
-
-
-
?
GTP + ADP
GDP + ATP
-
-
-
r
GTP + ADP
GDP + ATP
-
GDP is less effective dinucleotide substrate
-
r
GTP + ADP
GDP + ATP
-
-
-
r
GTP + ADP
GDP + ATP
-
-
-
r
GTP + ADP
GDP + ATP
-
reverse reaction at 62% the rate of UDP
-
r
GTP + ADP
GDP + ATP
-
-
-
r
GTP + ADP
GDP + ATP
-
-
-
r
GTP + ADP
GDP + ATP
-
-
-
r
GTP + ADP
GDP + ATP
-
-
-
r
GTP + CDP
GDP + CTP
-
-
-
-
?
GTP + CDP
GDP + CTP
-
-
-
r
GTP + GDP
GDP + GTP
-
in decreasing order of activity: UTP, dGTP, dTTP, CTP, dCTP, dUTP
-
?
GTP + GDP
GDP + GTP
-
equally active as ATP
-
?
GTP + GDP
GDP + GTP
-
-
-
?
GTP + NDP
GDP + NTP
-
-
-
-
?
GTP + NDP
GDP + NTP
-
-
-
-
?
GTP + nucleoside diphosphate
GDP + nucleoside triphosphate
-
-
-
-
?
GTP + nucleoside diphosphate
GDP + nucleoside triphosphate
-
-
-
-
?
GTP + nucleoside diphosphate
GDP + nucleoside triphosphate
-
-
-
-
?
GTP + UDP
GDP + UTP
-
-
-
-
?
GTP + UDP
GDP + UTP
-
-
-
-
?
ITP + ADP
IDP + ATP
-
-
-
?
ITP + ADP
IDP + ATP
-
reverse reaction at 42% the rate of UDP
-
?
ITP + ADP
IDP + ATP
-
-
-
?
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
may be involved in regulation of growth, development and signal-transduction processes
-
-
?
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
enzyme forms complexes with G protein beta gamma dimers and contributes to G protein activation
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
-
-
-
r
NDP + NTP
NTP + NDP
-
overview on roles in metabolic pathways and nucleic acid synthesis
-
-
?
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
Impatiens holstii
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
major component of nucleoside triphosphate synthetic pathway
-
-
?
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
involved in RNA- and DNA metabolism
-
-
?
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
-
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
autophosphorylation and phosphorylation of histone H1
-
-
?
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NDP + NTP
NTP + NDP
-
overview: functions in the cell
-
-
r
NTP + NDP
NDP + NTP
-
-
-
r
NTP + NDP
NDP + NTP
NDPK is a key metabolic enzyme that catalyzes the synthesis of non-adenine nucleoside triphosphate by transferring the terminal phosphate between NDP and NTP
-
-
r
NTP + NDP
NDP + NTP
-
-
-
r
NTP + NDP
NDP + NTP
the high-energy phosphate is mainly supplied by ATP, but the enzymes have broad substrate specificity and use the ribose and deoxyribose forms of both, purine and pyrimidine nucleotides, ping-pong mechanism with a phosphoenzyme/phosphohistidine intermediary involved in the transfer reaction, overview
-
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
no substrates are several nucleoside monophosphates
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the nature of the nucleoside triphosphate controls the rate of the reaction
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate 8-aza-UDP, human erythrocytic pI-variant 5.8
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
Impatiens holstii
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
Impatiens holstii
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
Impatiens holstii
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
Impatiens holstii
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
Impatiens holstii
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
Impatiens holstii
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
Impatiens holstii
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
Impatiens holstii
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
in decreasing order of activity: UTP, dTTP, CTP, dATP, ATP, dGTP, GTP
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrate: 5-fluoro-UTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
trinucleotide substrates: ATP, CTP, GTP, ITP, dTTP, UTP, dCTP, dGTP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
substrates: 6-aza-UDP, 8-aza-GDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
the broad specificity may reflect the presence of mixtures of isozymes of different reactivities with nucleotide substrates
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
dinucleotide substrates: ADP, CDP, GDP, UDP, IDP, dCDP, dGDP, dTDP
-
r
NTP + nucleoside diphosphate
NDP + NTP
-
broad specificity
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
many ribo- and deoxyribonucleoside triphosphates act as donors
-
?
NTP + nucleoside diphosphate
NDP + NTP
-
transfers gamma-phosphate from NTP to any nucleoside diphosphate
-
?
UTP + CDP
UDP + CTP
-
best substrate: UTP
-
-
?
UTP + CDP
UDP + CTP
-
in decreasing order of activity: dATP, ITP, dTTP, ATP, dGTP, GTP, dCTP, CTP
-
-
?
UTP + CDP
UDP + CTP
-
-
-
-
?
UTP + dCDP
UDP + dCTP
-
best substrate: UTP
-
-
?
UTP + dCDP
UDP + dCTP
-
in decreasing order of activity: ITP, dATP, dTTP, dGTP, GTP, ATP, CTP, dCTP
-
-
?
UTP + dCDP
UDP + dCTP
-
-
-
-
?
UTP + dTDP
UDP + dTDP
-
best substrate: UTP
-
-
?
UTP + dTDP
UDP + dTDP
-
in decreasing order of activity: ITP, dATP, dTTP, GTP, ATP, dGTP, dUTP, CTP, dCTP
-
-
?
UTP + dTDP
UDP + dTDP
-
best substrates
-
-
?
UTP + dTDP
UDP + dTDP
-
nucleoside triphosphates in decreasing order of activity: dTTP, CTP, dCTP, dATP, dGTP, GTP
-
-
?
UTP + dTDP
UDP + dTDP
-
nucleoside diphosphates in decreasing order of activity: CDP, UDP, GDP, dGDP, dCDP, dADP, ADP
-
-
?
additional information
?
-
-
role of enzyme in dUTP detoxification to compensate for the absence of a dUTPase in Mimivirus
-
-
?
additional information
?
-
-
preferential affinity for deoxypyrimidine nucleotides
-
-
?
additional information
?
-
-
the enzyme catalyzes transfer of the gamma-phosphate from a nucleoside triphosphate (NTP) to a nucleoside diphosphate (NDP)
-
-
?
additional information
?
-
-
NDPK is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Physical interaction between NDPK2 and small G proteins, as well as the heterotrimeric G protein GPA1. NDPK2 can stimulate their GTPase activities, but the dimeric form of NDPK2 is also unable to enhance the GTPase activities of both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins
-
-
?
additional information
?
-
NDPK is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Physical interaction between NDPK2 and small G proteins, as well as the heterotrimeric G protein GPA1. NDPK2 can stimulate their GTPase activities, but the dimeric form of NDPK2 is also unable to enhance the GTPase activities of both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins
-
-
?
additional information
?
-
-
NDPK2 is a H2O2 signaling protein that performs autophosphorylation. NDPK2 and H2O2 are involved in salt resistance, overview
-
-
?
additional information
?
-
nucleoside diphosphate kinase is a housekeeping enzyme that maintains the intracellular levels of all (d)NTPs used in biosynthesis except ATP, but it also plays a significant role in signal transduction pathways involved in oxidative stress. The enzyme protects the plants against reactive ocygen species, heat, and salt stress, overview
-
-
?
additional information
?
-
-
NDPK2 interacts with plant small G proteins in vitro, both Pra2 and Pra3both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins
-
-
?
additional information
?
-
NDPK2 interacts with plant small G proteins in vitro, both Pra2 and Pra3both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins
-
-
?
additional information
?
-
adenine nucleosides are used preferentially compared to cytidine nucleosides
-
-
-
additional information
?
-
-
adenine nucleosides are used preferentially compared to cytidine nucleosides
-
-
-
additional information
?
-
adenine nucleosides are used preferentially compared to cytidine nucleosides
-
-
-
additional information
?
-
the enzyme's primary function is to maintain dNTP pools by converting deoxynucleotide diphosphates to triphosphates using ATP as the major phosphate donor
-
-
?
additional information
?
-
-
the enzyme's primary function is to maintain dNTP pools by converting deoxynucleotide diphosphates to triphosphates using ATP as the major phosphate donor
-
-
?
additional information
?
-
strong preference for D-enantiomers of antiviral nucleotide analogs like ddATP, ddCTP, 3'-deoxy-3'-thymidine, 2',3'-didehydro-2',3'-dideoxythymidine
-
-
?
additional information
?
-
binding affinity of NDPs with the recombinant enzyme measured by isothermal titration calorimetry, indicate that the purines nucleotides show higher binding affinity compared with pyrimidines. The recombinant enzyme rNDPK has a definite nuclease activity in vitro, which cleaves supercoiled plasmid DNA, but has no effect on dsDNA and ssDNA. No or poor activity with CDP and UDP
-
-
?
additional information
?
-
-
binding affinity of NDPs with the recombinant enzyme measured by isothermal titration calorimetry, indicate that the purines nucleotides show higher binding affinity compared with pyrimidines. The recombinant enzyme rNDPK has a definite nuclease activity in vitro, which cleaves supercoiled plasmid DNA, but has no effect on dsDNA and ssDNA. No or poor activity with CDP and UDP
-
-
?
additional information
?
-
-
NDPK catalyzes the transfer of gamma-phosphate from nucleoside triphosphates to nucleoside diphosphates
-
-
?
additional information
?
-
substrate specificities of wild-type and G114R mutant enzymes, overview
-
-
?
additional information
?
-
critical role of the two amino acid residues, E134 and E135, at the carboxy-terminal region of NDK in determining the halophilic characteristics of the protein, overview
-
-
?
additional information
?
-
-
in the absence of nucleoside diphosphates the enzyme undergoes Mg2+-dependent stoichiometric autophosphorylation using ATP, GTP or gamma-thiotriphosphate as phosphate donor, 2 mol phosphate per mol enzyme
-
-
?
additional information
?
-
-
MDA-MB-435S cell-secreted NDPK-B supports tumour formation by modulating ATP levels locally to activate endothelial cell P2Y receptor-mediated angiogenesis, overview
-
-
?
additional information
?
-
NDPKA is a housekeeping enzyme essential for the synthesis of nonadenine nucleoside and deoxynucleoside 5'-triphosphate
-
-
?
additional information
?
-
-
NDPKA is a housekeeping enzyme essential for the synthesis of nonadenine nucleoside and deoxynucleoside 5'-triphosphate
-
-
?
additional information
?
-
-
the enzyme catalyzes transfer of the gamma-phosphate from a nucleoside triphosphate, NTP, to a nucleoside diphosphate, NDP
-
-
?
additional information
?
-
the transfer of high energetic phosphate by NDPK and/or the beta subunit of G proteins can induce G protein activation. Interaction of nucleoside diphosphate kinase B with heterotrimeric G protein betagamma dimers influencing the G protein activation and stability, mechanism, detailed overview
-
-
?
additional information
?
-
NDPK B acts as histidine kinase in a NDPK B/Gbetagamma complex, NDPK B/Gbetagamma complex formation, structure, and mode of action, overview. Nucleoside diphosphate kinase is a ubiquitous enzyme that catalyses the transfer of the gamma-phosphate from nucleoside 5'-triphosphates to nucleoside 5'-diphosphates by a ping-pong mechanism involving the formation of a high energy phosphate intermediate on His118
-
-
?
additional information
?
-
-
NDPK co-immunoprecipitates reciprocally with AMP-activated kinase and is co-localised with this enzyme in human airways
-
-
?
additional information
?
-
-
isoform NDPK-D is able to bind in a non-negligible manner to zwitterionic phosphatidylcholine
-
-
?
additional information
?
-
nucleoside diphosphate kinase B forms a functional complex with cell surface CFTR, binding analysis using peptide NDPK-B 36-54 (V36AMKFLRAS44EEHLKQHYID54), this region of NDPK-B, may constitute a key NDPK-B interaction domain for CFTR, the nucleotide binding domain 1 (NBD1, aa 351-727) of CFTR constitutes an NDPK-B interaction site with CFTR, surface plasmon resonance and NDPK-B and NBD1 protein-protein docking analysis
-
-
?
additional information
?
-
-
NdK is a stable enzyme capable of decreasing extracellular ATP levels
-
-
?
additional information
?
-
-
both recombinant and native Leishmania NdK utilized ATP to produce expected nucleoside triphosphates in the presence of nucleoside diphosphates in excess
-
-
?
additional information
?
-
-
NdK is a stable enzyme capable of decreasing extracellular ATP levels
-
-
?
additional information
?
-
-
both recombinant and native Leishmania NdK utilized ATP to produce expected nucleoside triphosphates in the presence of nucleoside diphosphates in excess
-
-
?
additional information
?
-
-
in the absence of nucleoside diphosphates the enzyme undergoes Mg2+-dependent stoichiometric autophosphorylation using ATP, GTP or gamma-thiotriphosphate as phosphate donor, 2 mol phosphate per mol enzyme
-
-
?
additional information
?
-
-
NDPK co-immunoprecipitates reciprocally with AMP-activated kinase and is co-localised with this enzyme in human airways
-
-
?
additional information
?
-
catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments
-
-
?
additional information
?
-
-
catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments
-
-
?
additional information
?
-
-
the enzyme binds to and inactivates the small GTPase Rac1 in the macrophage. This results in the exclusion of the Rac1 binding partner p67phox from phagosomes containing bacteria- or enzyme-coated latex beads. Exclusion of p67phox is associated with a defect of both NOX2 assembly and production of reactive oxygen species in response to wild-type bacteria
-
-
?
additional information
?
-
catalyzes cleavage of single strand DNA, oligonucleotide P1 used for experiments
-
-
?
additional information
?
-
NDK-1 plays an important role in protecting conidial viability under light stress in Neurospora crassa
-
-
?
additional information
?
-
-
NDK-1 plays an important role in protecting conidial viability under light stress in Neurospora crassa
-
-
?
additional information
?
-
-
NDPK co-immunoprecipitates reciprocally with AMP-activated kinase and is co-localised with this enzyme in human airways
-
-
?
additional information
?
-
enzyme LvNDK has relaxed substrate specificity and binds both purine and pyrimidine deoxynucleoside diphosphates with high binding affinity for dGDP and dADP and with low binding interaction for dCDP. The binding of deoxy- or ribonucleotides is similar, as in the former a water molecule replaces the hydrogen bond made by Lys11 to the 20-hydroxyl group of the ribose moiety. This allows Lys11 to maintain a catalytically favourable conformation independently of the kind of sugar found in the nucleotide
-
-
?
additional information
?
-
-
enzyme LvNDK has relaxed substrate specificity and binds both purine and pyrimidine deoxynucleoside diphosphates with high binding affinity for dGDP and dADP and with low binding interaction for dCDP. The binding of deoxy- or ribonucleotides is similar, as in the former a water molecule replaces the hydrogen bond made by Lys11 to the 20-hydroxyl group of the ribose moiety. This allows Lys11 to maintain a catalytically favourable conformation independently of the kind of sugar found in the nucleotide
-
-
?
additional information
?
-
-
enzyme additionally has nuclease activity cleaving supercoiled plasmid DNA, tRNAs or the 3UTR of atp9 mRNA. Nuclease- and kinase mechanisms are separate
-
-
?
additional information
?
-
-
key enzyme involved in the homeostasis of nucleoside triphosphates
-
-
?
additional information
?
-
key enzyme involved in the homeostasis of nucleoside triphosphates
-
-
?
additional information
?
-
-
nucleoside diphosphate kinase is responsible for maintaining the nucleotide balance in the cell, but is also involved in regulatory functions that often are mediated via protein-protein interactions, differentially for adenylate kinase isozymes, overview
-
-
?
additional information
?
-
-
NDPK3 protein additionally cleaves not only supercoiled plasmid DNA, but also highly structured RNA molecules such as tRNAs or the 3'UTR of the atp9 mRNA suggesting that the NDPK3 nuclease activity has a structural requirement, ATP inhibits this nuclease activity, while ADP has no effect, the nuclease- and the kinase-mechanisms are separate, overview
-
-
?
additional information
?
-
-
protein-protein interaction analysis of adenylate kinases from Arabidopsis thaliana with the immobilized recombinant His-tagged NDPK from Pisum sativum, the interaction influences both enzyme activities, overview
-
-
?
additional information
?
-
-
the binding affinity of different nucleotides with Plasmodium falciparum nucleoside diphosphate kinase is varied according to the following order ADP/GDP > dGDP > dADP > dTDP > CDP > dCDP >U DP. The enzyme shows more preferences to ribonucleotides over deoxyribonucleotides
-
-
?
additional information
?
-
-
coupling of the formation of ATP to phenazine methosulfate and iodonitrotetrazolium chloride, coupling is achieved using hexokinase and G6PDH, which produce the necessary NADPH molecules for formazan precipitation at the site of enzyme activity, method optimization, overview, measurement of the reverse enzyme reactions, overview
-
-
?
additional information
?
-
-
in the absence of nucleoside diphosphates the enzyme undergoes Mg2+-dependent stoichiometric autophosphorylation using ATP, GTP or gamma-thiotriphosphate as phosphate donor, 2 mol phosphate per mol enzyme
-
-
?
additional information
?
-
-
enzyme is involved in receptor-independent regulation of cAMP synthesis and contractility in intact cardiomyocytes and has histidine kinase activity for phosphorylation of G protein subunit beta phosphorylation at H266
-
-
?
additional information
?
-
-
In addition to the receptor-induced GDP/GTP exchange, G protein alpha subunits can be activated by a phosphate transfer via a plasma membrane-associated complex of NDPK B and G protein betagamma-dimers, Gbetagamma, requiring the intermediate phosphorylation of His266, the histidine kinase activity of NDPK B for Gbeta is important for the receptor-independent regulation of cAMP synthesis and contractility in intact cardiomyocytes, overview
-
-
?
additional information
?
-
-
the enzyme is involved in endoplasmic reticulum membranes structure formation, the mechanism of membrane extension involves direct lipid binding, as NDKB-bound phosphatidylinositol-4-phosphate, phosphatidylinositol-4,5-diphosphate, and phosphatidic acid. Binding to these anionic lipids requires clusters of basic residues on the surface of the NDKB hexamer. Amino acid changes in NDKB, that block lipid binding, also block endoplasmic reticulum network extension, overview
-
-
?
additional information
?
-
-
the transfer of high energetic phosphate by NDPK and/or the beta subunit of G proteins can induce G protein activation. Interaction of nucleoside diphosphate kinase B with heterotrimeric G protein betagamma dimers influencing the G protein activation and stability, mechanism, detailed overview
-
-
?
additional information
?
-
-
interaction of recombinant NDP kinases alpha and beta and their mutant chimeric derivatives with rhodopsin-transducin complex, binding to ROS photoreceptor membranes, overview
-
-
?
additional information
?
-
-
NDKB stabilizes an extended endoplasmic reticulum network in vitro, overview
-
-
?
additional information
?
-
-
NDPK B acts as histidine kinase in a NDPK B/Gbetagamma complex, NDPK B/Gbetagamma complex formation, structure, and mode of action, overview. Nucleoside diphosphate kinase is a ubiquitous enzyme that catalyses the transfer of the gamma-phosphate from nucleoside 5'-triphosphates to nucleoside 5'-diphosphates by a ping-pong mechanism involving the formation of a high energy phosphate intermediate on His118
-
-
?
additional information
?
-
-
autophosphorylation and phosphorylation of histone H1
-
-
?
additional information
?
-
NDPK1 exhibits nuclease activity cleaving not only linear DNA, but also supercoiled plasmid DNA, ATP or ADP do not affect the nuclease activity while the absence of Mg2+ completely inhibits this activity
-
-
?
additional information
?
-
NDPK1 exhibits nuclease activity cleaving not only linear DNA, but also supercoiled plasmid DNA, ATP or ADP do not affect the nuclease activity while the absence of Mg2+ completely inhibits this activity
-
-
?
additional information
?
-
-
NDPK1 exhibits nuclease activity cleaving not only linear DNA, but also supercoiled plasmid DNA, ATP or ADP do not affect the nuclease activity while the absence of Mg2+ completely inhibits this activity
-
-
?
additional information
?
-
the enzyme has nuclease activity and DNA-binding properties in vitro
-
-
-
additional information
?
-
-
the enzyme has nuclease activity and DNA-binding properties in vitro
-
-
-
additional information
?
-
the enzyme has nuclease activity and DNA-binding properties in vitro
-
-
-
additional information
?
-
kinetic parameters and regulation mechanisms of NDPK1 and NDPK2, overview
-
-
?
additional information
?
-
-
sequential ordered bi-bi reaction mechanism. The enzyme additionally shows dTMP kinase activity, reaction of EC 2.7.4.9, using dTMP as a substrate to produce dTDP
-
-
?
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E28A
-
site-directed mutagenesis the mutation interrupts the secondary structure of the protein leading to declined enzymatic activity compared to wild-type
LP175S
no interaction between NDPK2 and small G proteins, EC 3.6.5.2
H117Q
then active site mutant H117Q loses kinase activity
V83F
the structure of the mutant enzyme is not stable at higher temperature
H117Q
-
then active site mutant H117Q loses kinase activity
-
V83F
-
the structure of the mutant enzyme is not stable at higher temperature
-
G19V
the mutation rigidifies the 43-63 loop in Ndk, using GTP as the phosphate donor a 300fold reduction in phosphotransferase activity is observed
H115Q
H115Q is incompetent in autophosphorylation, confirming the role of histidine as a phosphate acceptor
E136T
the mutant forms a dimer
G134A/E136T
the mutant forms a tetramer
G134A/E136T/C139S
the mutant forms a tetramer
G134A/N135A/E136T
the mutant forms a tetramer
G134E
the mutant forms a dimer
G134E/E136T
the mutant forms a dimer
G134EA
the mutant forms a dimer
G134A/E136T
-
the mutant forms a tetramer
-
G134A/N135A/E136T
-
the mutant forms a tetramer
-
G134E
-
the mutant forms a dimer
-
G134EA
-
the mutant forms a dimer
-
H122G
study of chemical rescue for ATP attack, crystallization data
K16A
-
specific activity drastically reduced
K16R
-
specific activity drastically reduced
Y56A
-
specific activity drastically reduced
R31C
-
the mutation destabilizes the hexameric assembly, and causes dissociation to less active dimers at low salt concentrations
D112S
-
the mutant shows reduced specific activity compared to the wild type enzyme
D148C
-
the mutation enhances stability and folding in low salt solution by S-S bond. The mutant shows increased thermal stability by about 10°C in 0.2 M NaCl over the wild type enzyme
D58G
-
the mutant shows strongly reduced specific activity compared to the wild type enzyme
D58G/D63G
-
the mutant shows no refolding enhancement and strongly reduced specific activity compared to the wild type enzyme
D63G
-
the mutant shows slightly reduced specific activity compared to the wild type enzyme
G114K
active mutant enzyme, refolds in 1M NaCl after heat-denaturation, under which the wild-type enzyme and mutant enzyme G114S proteins show no refolding
G114S
active mutant enzyme, dissociates as extensively as the wild-type enzyme as low salt buffer
N111L
a HisN111L mutant with an N-terminal extension sequence containing hexa-His tag has enhanced salt requirements for enzymatic activity and refolding even though the secondary structure of the HisN111L mutant is confirmed to be similar to the control
G114D
-
inactive mutant enzyme, dissociates more than wild-type enzyme in low salt buffer
-
G114K
-
active mutant enzyme, refolds in 1M NaCl after heat-denaturation, under which the wild-type enzyme and mutant enzyme G114S proteins show no refolding
-
G114R
-
once folded, the mutant enzyme is stable even in low salt buffer
-
G114S
-
active mutant enzyme, dissociates as extensively as the wild-type enzyme as low salt buffer
-
N111L
-
a HisN111L mutant with an N-terminal extension sequence containing hexa-His tag has enhanced salt requirements for enzymatic activity and refolding even though the secondary structure of the HisN111L mutant is confirmed to be similar to the control
-
C139S
site-directed mutagenesis, the mutation in the dimeric nucleoside diphosphate kinase generates a catalytically competent enzyme monomer
E134A/E135A
site-directed mutagenesis, the mutant shows increased oligomerization compared to the wild-type enzyme
H118C
-
the mutant of isozyme NDPKB is catalytically inactive
H118G/F60W
-
mutant engineered for following intrinsic fluorescence during substrate binding, crystallization data
H118N/S120G
site-directed mutagenesis, the mutant is inactive and expressed as insoluble protein in inclusion bodies
K135L
-
isoform B, mutation in dimeric interface, defective DNA-binding and reduced stability of enzyme
N69H
-
isoform B, mutation in dimeric interface, defective DNA-binding and reduced stability of enzyme
R34G
-
isoform B, mutation in dimeric interface, defective DNA-binding and reduced stability of enzyme
R90D
site-directed mutagenesis, the mutant does not bind to cardiolipin
H117A
no effect on nuclease activity
H117Q
-
significant loss of activity with ATP and GTP
H49Q
-
no effect on activity
H52A
unable to complement NdK deficient Pseudomonas aeruginosa, Km values similar to wild type protein but significantly reduced turnover
K29A
no phosphotransfer activity
R104A
no phosphotransfer activity
R80A
less stable than wild-type enzyme. Mutation abolishes the six intersubunit salt bridges. Compensatory stabilizing mechanisms appear for R80A mutant. The R80A mutant crystallizes into space group I222 that is unusual for nucleoside diphosphate kinases, and its hexameric structure reveals the occurrence at the trimer interface of a stabilizing hydrophobic patch around the mutation. The Tm-value of the mutant enzyme is 69.0°C, compared to 76.1°C for the wild-type enzyme
R86A
no phosphotransfer activity
Y50F
unable to complement NdK deficient Pseudomonas aeruginosa, no phosphotransfer activity
D93N
-
less stable than wild-type enzyme. The Tm-value of the mutant enzyme is 48.4°C, compared to 76.1°C for the wild-type enzyme
-
R80A
-
less stable than wild-type enzyme. Mutation abolishes the six intersubunit salt bridges. Compensatory stabilizing mechanisms appear for R80A mutant. The R80A mutant crystallizes into space group I222 that is unusual for nucleoside diphosphate kinases, and its hexameric structure reveals the occurrence at the trimer interface of a stabilizing hydrophobic patch around the mutation. The Tm-value of the mutant enzyme is 69.0°C, compared to 76.1°C for the wild-type enzyme
-
R80N
-
less stable than wild-type enzyme. Mutation abolishes the six intersubunit salt bridges. The Tm-value of the mutant enzyme is 69.3 °C, compared to 76.1°C for the wild-type enzyme
-
H52A
-
unable to complement NdK deficient Pseudomonas aeruginosa, Km values similar to wild type protein but significantly reduced turnover
-
H53Q
-
unable to complement NdK deficient Pseudomonas aeruginosa, Km values similar to wild type protein but significantly reduced turnover
-
K29A
-
no phosphotransfer activity
-
R104A
-
no phosphotransfer activity
-
Y50F
-
unable to complement NdK deficient Pseudomonas aeruginosa, no phosphotransfer activity
-
D93N
-
the mutation dramatically decreases protein thermal stability (Tm value of 43.4°C) but is still hexameric and retains full activity. The mutant hexamer is stable and active up to 2.5 M guanidine hydrochloride
-
H117A
-
no effect on nuclease activity
-
H117Q
-
significant loss of activity with ATP and GTP
-
H49Q
-
no effect on activity
-
H53Q
-
no effect on activity
-
K10A
-
nuclease activity completely abolished
-
P72H
-
mutant strain is hypersensitive to oxidative and heat stress and shows a decrease in the levels of catalase isoforms Cat-1 and Cat-3 induced by stress and illumination. Catalase Cat-1 interacts with nucleoside diphosphate kinase
A117D
-
complete loss of nucleoside diphosphate kinase activity, but no loss of nuclease activity
A77T
site-directed mutagenesis, the mutant shows an altered import behaviour into chloroplasts compared to the wild-type enzyme, overview
E205K
the mutation of isoform NDPK2 is associated with paraquat resistance
H117A
activity almost completely abolished
H117D/S119A
activity almost completely abolished
I12L
the mutation of isoform NDPK2 is associated with paraquat resistance
P45S
the mutation of isoform NDPK3 is associated with paraquat resistance
R63D
site-directed mutagenesis, the mutant shows an altered import behaviour into chloroplasts compared to the wild-type enzyme, overview
R74D
site-directed mutagenesis, the mutant shows an altered import behaviour into chloroplasts compared to the wild-type enzyme, overview
S119A
reduced serine phosphorylation, only modest decrease of activity
S69A/S119A
activity almost completely abolished
H117Q
site-directed mutagenesis, inactive mutant
A134E
-
site-directed mutagenesis, the mutant forms a dimer in analogy to the wild-type enzyme of Halomonas sp., which possesses an E134 residue, overview
A134E/A135E
-
site-directed mutagenesis, the mutant shows decreased oligomerization compared to the wild-type enzyme
H266L
-
generation of a Gbeta1gamma2-dimer carrying a single amino acid exchange at the intermediately phosphorylated His266 in the beta1 subunit, the mutant is termed Gbeta1H266Lgamma2, the mutant is integrated into heterotrimeric G proteins in rat cardiomyocytes but is deficient in intermediate Gbeta phosphorylation, mutant overexpression suppresses basal cAMP formation as well as the basal contractility up to 50-55% compared to the wild-type enzyme, overview
K56E/R58E
-
site-directed mutation of the residues required for membrane phospholipid binding, the NDKB mutant is defective in anionic phospholipid binding
H117N
site-directed mutagenesis, the mutant His117N fused with GFP is inactive
P95S
site-directed mutagenesis, mutation in Pro95 and in the C-terminus destabilize the hexamer fused with GFP
H117N
-
site-directed mutagenesis, the mutant His117N fused with GFP is inactive
-
P95S
-
site-directed mutagenesis, mutation in Pro95 and in the C-terminus destabilize the hexamer fused with GFP
-
H197C
NDPK2 kinase site mutant
H197C
no enzymatic activity, stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, in the same manner as wild-type NDPK2
L225stop
C-terminal deletion mutant
L225stop
increased enzymatic activity, stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, in the same manner as wild-type NDPK2
P175S
NDPK2 kinase site mutant
P175S
stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, with a slight reduction compared to wild-type NDPK2
R230stop
C-terminal deletion mutant
R230stop
increased enzymatic activity, stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, in the same manner as wild-type NDPK2
C139S
the mutant forms a dimer
C139S
site-directed mutagenesis, the mutation in the dimeric nucleoside diphosphate kinase generates a catalytically competent enzyme monomer. Substitution of Cys139 for Ser causes dissociation of dimeric CsNDK into monomer in Tris buffer, and the mutant CsNDK becomes more susceptible to endproteinase GluC cleavage, which is suppressed by an NDK substrate, ATP
C139S
-
the mutant forms a dimer
-
C139S
-
site-directed mutagenesis, the mutation in the dimeric nucleoside diphosphate kinase generates a catalytically competent enzyme monomer. Substitution of Cys139 for Ser causes dissociation of dimeric CsNDK into monomer in Tris buffer, and the mutant CsNDK becomes more susceptible to endproteinase GluC cleavage, which is suppressed by an NDK substrate, ATP
-
G114D
-
the mutant, which forms a dimer in low salt solution, is strongly stabilized by His-tag to form a stable hexamer and shows strongly reduced specific activity compared to the wild type enzyme
G114D
inactive mutant enzyme, dissociates more than wild-type enzyme in low salt buffer
G114R
wild-type enzyme is a hexamer at 25°C and dissociates to dimer at 35°C in low salt medium. Mutant maintains hexameric structure at both 25 and 35°C. Refolding of heat-denatured wild-type enzyme requires salt concentrations above 2 M. Mutant G114R efficiently refolds in presence of 1 M NaCl. Residue 114 is in close proximity to E155 of the neighboring subunit in the enzyme hexamer. In the mutant, R114 may stabilize the hexameric subunit assembly
G114R
random mutagenesis, the mutant enzyme shows increased thermal stability and refolding after salt treatment compared to the wild-type enzyme, substrate specificity and kinetic of the mutant enzyme, overview
G114R
once folded, the mutant enzyme is stable even in low salt buffer
E134A
site-directed mutagenesis, the mutant forms a tetramer in analogy to the wild-type enzyme of Pseudomonas sp., which possesses an A134 residue, overview
E134A
the mutant forms a tetramer and shows 119% specific activity compared to the wild type enzyme
S120G
-
mutant identified in aggressive neuroblastomas, crystallization data
S120G
naturally mutation occuring in neuroblastoma, the mutant has a folding defect, the urea-denatured protein cannot refold in vitro
D93N
the mutation dramatically decreases protein thermal stability (Tm value of 43.4°C) but is still hexameric and retains full activity. The mutant hexamer is stable and active up to 2.5 M guanidine hydrochloride
D93N
-
site-directed mutagenesis, the mutation breaks the intersubunit salt bridge Arg80-Asp93 which contributes to the thermal stability of the hexamer. The mutant thermal stability dramatically decreases by 27.6°C to 48.4°C, compared to 76°C for the wild-type enzyme
D93N
less stable than wild-type enzyme. The Tm-value of the mutant enzyme is 48.4°C, compared to 76.1°C for the wild-type enzyme
H53Q
-
no effect on activity
H53Q
unable to complement NdK deficient Pseudomonas aeruginosa, Km values similar to wild type protein but significantly reduced turnover
K10A
no phosphotransfer activity
K10A
nuclease activity completely abolished
R80N
-
site-directed mutagenesis, the mutation affects the intersubunit salt bridge Arg80-Asp93 which contributes to the thermal stability of the hexamer. The mutant thermal stability dramatically decreases by 8.0°C to 68°C, compared to 76°C for the wild-type enzyme. In mutant R80N, the salt bridge is replaced by intersubunit hydrogen bonds that contribute to the thermal stability of the hexamer
R80N
less stable than wild-type enzyme. Mutation abolishes the six intersubunit salt bridges. The Tm-value of the mutant enzyme is 69.3 °C, compared to 76.1°C for the wild-type enzyme
H117Q
-
the mutation abolishes the ATPase and GTPase activities of the recombinant enzyme in vitro to almost 4.75- and 2fold, respectively. The mutation also reduces the autophosphorylation activity, almost 5fold
H117Q
-
the mutation abolishes the ATPase and GTPase activities of the recombinant enzyme in vitro to almost 4.75- and 2fold, respectively. The mutation also reduces the autophosphorylation activity, almost 5fold
-
S69A
6% and 14% of wild type activity using dCDP or dTDP as substrate respectively, changes in subunit composition with increasing number of tetramers and dimers
S69A
-
complete loss of nucleoside diphosphate kinase activity, but no loss of nuclease activity
D135A/E136T
-
site-directed mutagenesis
D135A/E136T
-
site-directed mutagenesis
-
additional information
-
construction of a C-terminally truncated mutant, truncation of the C-terminal arginine-threonine-arginine (RTR) residues leads to the instability of the tertiary structure resulting in reduced kinase activity compared to wild-type, mutant crystal structure analysis
additional information
-
a ndpk2 knockout mutant shows increased salt stress sensitivity compared to the wild-type enzyme. The sos2-2/ndpk2 double mutant is more salt sensitive than a sos2-2 single mutant, and does not hyperaccumulate H2O2 in response to salt stress
additional information
-
NDPK3a induction by sugars is reduced in AtWrky4 and AtWrky34 knockout mutants, Wrky4 and Wrky34 are transcription factor proteins involved in sugar induction and response, overview
additional information
transgenic Solanum tubersoum plants overexpressing the Arabidopsis thaliana NDPK2 isozyme show reduced sensitivity to stress caused by methyl viologen, i.e. reactive oxigen species, or salt or heat, compared to the wild-type plants, overview
additional information
construction of NDPK3 knockout and overexpression lines: in the NDPK-3 overexpressor, the enzyme activities of ascorbate peroxidase, peroxiredoxin, catalase, as well as superoxide dismutase are all increased compared to wild-type, in the NDPK-3 knockout line, these enzymes have reduced activity compared to wild-type. Proteomic analysis of mutants, overview
additional information
-
construction of NDPK3 knockout and overexpression lines: in the NDPK-3 overexpressor, the enzyme activities of ascorbate peroxidase, peroxiredoxin, catalase, as well as superoxide dismutase are all increased compared to wild-type, in the NDPK-3 knockout line, these enzymes have reduced activity compared to wild-type. Proteomic analysis of mutants, overview
-
additional information
-
antisense knockdown of nucleoside diphosphate kinase, the stable mutant Mtb Ndk-AS displays attenuated intracellular survival along with reduced persistence in the lungs of infected mice. In the generated mutant strain Mtb Ndk-AS the Ndk protein expression is undetectable. ROS production is inhibited in the presence of Mtb Ndk
additional information
construction of CAT-1 and NDK-1 double mutants, cat-1/ndk-1-1 and cat-1/ndk-1-2, by crossing single mutants of cat-1RIP and ndk-1P72H, which are catalytically inactive, overview
additional information
-
construction of CAT-1 and NDK-1 double mutants, cat-1/ndk-1-1 and cat-1/ndk-1-2, by crossing single mutants of cat-1RIP and ndk-1P72H, which are catalytically inactive, overview
additional information
-
transgenic plants bearing gene for enzyme antisense RNA, inhibition of cell elongation process
additional information
-
immobiliztaion of the purified recombinant enzyme on N-hydroxysuccinimide HS-activated HP resin
additional information
the Pyrobaculum aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66100 and 156165. Deletion mutants of the NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor. A structural analysis of the evolved NDP kinase in conjunction with mutagenesis experiments suggests that the substrate specificity of the enzyme is related to the presence of these two inserts
additional information
-
the Pyrobaculum aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66100 and 156165. Deletion mutants of the NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor. A structural analysis of the evolved NDP kinase in conjunction with mutagenesis experiments suggests that the substrate specificity of the enzyme is related to the presence of these two inserts
additional information
-
the Pyrobaculum aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66100 and 156165. Deletion mutants of the NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor. A structural analysis of the evolved NDP kinase in conjunction with mutagenesis experiments suggests that the substrate specificity of the enzyme is related to the presence of these two inserts
-
additional information
-
construction of chimeric mutants of NDP kinase alpha and NDP kinase beta, i.e. NDP kinase alpha1-130beta131-152 and NDP kinase beta1-130alpha131-152
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