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IUBMB CommentsInvolved in biosynthesis of the aminoglycoside antibiotic neomycin. Requires a divalent metal ion, optimally Mg2+, Mn2+ or Co2+.
The enzyme appears in viruses and cellular organisms
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neoK
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UDP-N-acetyl-alpha-D-glucosamine + ribostamycin = UDP + 2'''-acetyl-6'''-hydroxyneomycin C
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UDP-N-acetyl-alpha-D-glucosamine:ribostamycin N-acetylglucosaminyltransferase
Involved in biosynthesis of the aminoglycoside antibiotic neomycin. Requires a divalent metal ion, optimally Mg2+, Mn2+ or Co2+.
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UDP-N-acetyl-alpha-D-glucosamine + ribostamycin
UDP + 2'''-acetyl-6'''-hydroxyneomycin C
UDP-N-acetyl-alpha-D-glucosamine + ribostamycin
UDP + 2'''-acetyl-6'''-hydroxyneomycin C
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UDP-N-acetyl-alpha-D-glucosamine + ribostamycin
UDP + 2'''-acetyl-6'''-hydroxyneomycin C
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Mg2+
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Mg2+
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100% activity in the presence of 2 mM
Mn2+
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Mn2+
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105% activity in the presence of 2 mM Mn2+ compared to Mg2+
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Ca2+
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about 55% residual activity in the presence of 2 mM
Co2+
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about 90% residual activity in the presence of 2 mM
EDTA
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about 20% residual activity in the presence of 10 mM
Ni2+
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about 80% residual activity in the presence of 2 mM
Cu2+
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inhibits activity
Cu2+
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complete inhibition in the presence of 2 mM
Zn2+
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inhibits activity
Zn2+
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complete inhibition in the presence of 2 mM
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brenda
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brenda
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metabolism
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NeoK is a glycosyltransferase that catalyzes the retaining glycosylation of ribostamycin with UDP-GlcNAc as a sugar donor
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NEOK_STRFR
366
0
39257
Swiss-Prot
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A0A1Y2SS22_9GAMM
340
0
38923
TrEMBL
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A0A1D8G9U5_9ACTN
366
0
39205
TrEMBL
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A0A840QEG2_9PSEU
328
0
36041
TrEMBL
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A0A1Y2NSQ1_STRFR
366
0
39257
TrEMBL
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DEAE Sepharose column chromatography, Superdex Hi-Load 200 gel filtration, and Mono Q column chromatography
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purified to homogeneity by DEAE Sepharose column chromatography, gel filtration, and MonoQ column chromatography
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expressed as a His-tagged fusion protein in Escherichia coli
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expressed in Escherichia coli BL21(DE3) cells
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Yokoyama, K.; Yamamoto, Y.; Kudo, F.; Eguchi, T.
Involvement of two distinct N-acetylglucosaminyltransferases and a dual-function deacetylase in neomycin biosynthesis
ChemBioChem
9
865-869
2008
Streptomyces sp., Streptomyces fradiae
brenda
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