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IUBMB Comments Involved in the biosynthesis of vindoline from tabersonine in the Madagascar periwinkle Catharanthus roseus.
The expected taxonomic range for this enzyme is: Catharanthus roseus
Synonyms
16-methoxy-2,3-dihydro-3-hydroxytabersonine N-methyltransferase, methyltransferase, 16-methoxy-2,3-dihydro-3-hydroxytabersonine, NMT, S-adenosyl-L-methionine:16-methoxy-2,3-dihydro-3-hydroxy-tabersonine N-methyltransferase,
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16-methoxy-2,3-dihydro-3-hydroxytabersonine N-methyltransferase
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methyltransferase, 16-methoxy-2,3-dihydro-3-hydroxytabersonine
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S-adenosyl-L-methionine:16-methoxy-2,3-dihydro-3-hydroxy-tabersonine N-methyltransferase
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S-adenosyl-L-methionine + 3-hydroxy-16-methoxy-2,3-dihydrotabersonine = S-adenosyl-L-homocysteine + deacetoxyvindoline
S-adenosyl-L-methionine + 3-hydroxy-16-methoxy-2,3-dihydrotabersonine = S-adenosyl-L-homocysteine + deacetoxyvindoline
summerization of biosynthetic pathway of vindoline
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S-adenosyl-L-methionine + 3-hydroxy-16-methoxy-2,3-dihydrotabersonine = S-adenosyl-L-homocysteine + deacetoxyvindoline
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methyl group transfer
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S-adenosyl-L-methionine:3-hydroxy-16-methoxy-2,3-dihydrotabersonine N-methyltransferase
Involved in the biosynthesis of vindoline from tabersonine in the Madagascar periwinkle Catharanthus roseus.
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S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
S-adenosyl-L-homocysteine + deacetoxyvindoline
additional information
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hydrogenation of 2,3 double bond of tabersonine instead of hydration results in a 75% decrease in N-methylation, double bound present: no N-methylation, the presence of 6,7 double bound is a necessary structural requirement for N-methylation with membrane-bound enzyme, whereas the detergent-solubilized enzyme is also able to N-methylate the 6,7 anhydro analogue
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S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
S-adenosyl-L-homocysteine + deacetoxyvindoline
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i.e. N(1)-methyl-16-methoxy-2,3-dihydro-3-hydroxytabersonine
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S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
S-adenosyl-L-homocysteine + deacetoxyvindoline
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i.e. N(1)-methyl-16-methoxy-2,3-dihydro-3-hydroxytabersonine
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S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
S-adenosyl-L-homocysteine + deacetoxyvindoline
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high degree of specificity for the N-indole of 2,3-dihydro-3-hydroxytabersonine
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S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
S-adenosyl-L-homocysteine + deacetoxyvindoline
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enzyme is involved in the biosynthesis of vindoline from tabersonine
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S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
S-adenosyl-L-homocysteine + deacetoxyvindoline
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enzyme is involved in the biosynthesis of vindoline from tabersonine
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S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
S-adenosyl-L-homocysteine + deacetoxyvindoline
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enzyme is involved in the biosynthesis of vindoline from tabersonine
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
S-adenosyl-L-homocysteine + deacetoxyvindoline
S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
S-adenosyl-L-homocysteine + deacetoxyvindoline
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enzyme is involved in the biosynthesis of vindoline from tabersonine
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S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
S-adenosyl-L-homocysteine + deacetoxyvindoline
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enzyme is involved in the biosynthesis of vindoline from tabersonine
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S-adenosyl-L-methionine + 16-methoxy-2,3-dihydro-3-hydroxytabersonine
S-adenosyl-L-homocysteine + deacetoxyvindoline
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enzyme is involved in the biosynthesis of vindoline from tabersonine
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lysolecithin
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completely elimination of activity
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CHAPS
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highest activity with 3 mg/ml
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additional information
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brenda
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brenda
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brenda
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associated with thylakoids
brenda
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NMT_CATRO
289
0
31789
Swiss-Prot
other Location (Reliability: 2 )
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60000
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gel chromatography
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reducing agent, e.g. DTT, necessary for the retention of activity after breakage of thylakoid membranes of chloroplasts
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Triton X-100
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0.1% Triton X-100 90% of activity
additional information
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octyl-beta-D-glucoside: 0.15% solution 100% of activity
additional information
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optimal pH for solubilization: 7.5
additional information
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CHAPS: 232% activity is solubilized
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labile to O2, DTT or 2-mercaptoethanol stabilizes
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485690
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-20°C, in absence of CHAPS, 4 days, 50% loss of activity
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-20°C, in absence of detergent by adding 25% glycerol, one week, 9% loss of activity
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-20°C, in presence of 0.6% CHAPS, 7 days, 50% loss of activity
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De Luca, V.; Cutler, A.J.
Subcellular localization of enzymes involved in indole alkaloid biosynthesis in Catharanthus roseus
Plant Physiol.
85
1099-1102
1987
Catharanthus roseus
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De Luca, V.; Balsevich, J.; Tyler, R.T.; Eilert, U.; Panchuk, B.D.; Kurz, W.G.W.
Biosynthesis of indole alkaloids: developmental regulation of the biosynthethic pathway from tabersonine to vindoline in Catheranthus roseus
J. Plant Physiol.
125
147-156
1986
Catharanthus roseus
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brenda
Dethier, M.; De Luca, V.
Partial purification of an N-methyltransferase involved in vindoline biosynthesis in Catharanthus roseus
Phytochemistry
32
673-678
1993
Catharanthus roseus
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brenda
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