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S-adenosyl-L-methionine + Escherichia coli tRNA-Met
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in Escherichia coli tRNA-Met
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in Escherichia coli tRNA-Ile
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in Escherichia coli tRNA-Ile
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in Escherichia coli tRNA-Phe
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in Escherichia coli tRNA-Phe
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
S-adenosyl-L-methionine + guanosine18 in tRNAPhe
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNAPhe
S-adenosyl-L-methionine + guanosine18 in tRNATyr
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr
S-adenosyl-L-methionine + guanosine18 in yeast tRNA-Phe
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNA-Phe
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe mutant transcript
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-AGA mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-AGA mutant transcript
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-dG18 mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-dG18 mutant transcript
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-GAA mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-GAA mutant transcript
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-GGA mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-GGA mutant transcript
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-GUA mutant transcript
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-GUA mutant transcript
-
-
-
-
?
S-adenosyl-L-methionine + tRNA
S-adenosyl-L-homocysteine + 2-methylthio N6(gamma,gamma,dimethylallyl)adenosine in tRNA
S-adenosyl-L-methionine + tRNA
S-adenosyl-L-homocysteine + tRNA containing 2'-O-methylguanine
S-adenosyl-L-methionine + yeast tRNA-Phe
S-adenosyl-L-homocysteine + yeast tRNA-Phe containing 2'-O-methylguanine
-
-
-
-
?
additional information
?
-
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
unmethylated tRNATyr
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
unmethylated tRNATyr
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
Marinobacter nauticus
-
-
the organisms' tRNA contains a G residue at the 18th position adjacent to the anticodon loop, except tRNAMet, tRNAPro, and tRNAVal
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
-
the organisms' tRNA contains a G residue at the 18th position adjacent to the anticodon loop, except tRNAMet, tRNAPro, and tRNAVal
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
-
the organisms' tRNA contains a G residue at the 18th position adjacent to the anticodon loop, except tRNAMet, tRNAPro, and tRNAVal
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
-
the organisms' tRNA contains a G residue at the 18th position adjacent to the anticodon loop, except tRNAMet, tRNAPro, and tRNAVal
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
Q9FAC4
methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptor from yeast: tRNAPhe
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptor from yeast: tRNAPhe
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptor from yeast: tRNAPhe
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptor from yeast: tRNAPhe
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptors from Bacillus subtilis: tRNAVal, tRNALys, tRNAThr, tRNALeu
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
substrate tRNAs from E. coli, yeast, Bacillus subtilis
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptor from yeast: tRNAPhe
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptor from yeast: tRNAPhe
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptor from yeast: tRNAPhe
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptors from Bacillus subtilis: tRNAVal, tRNALys, tRNAThr, tRNALeu
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptor from yeast: tRNAPhe
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptor from yeast: tRNAPhe
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
substrate tRNAs from E. coli, yeast, Bacillus subtilis
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptor from yeast: tRNAPhe
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA
-
methyl group acceptor from yeast: tRNAPhe
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNAPhe
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNAPhe
substrate yeast tRNAPhe
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNAPhe
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNAPhe
substrate yeast tRNAPhe
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNATyr
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr
-
involved in post-transcriptional tRNA modification
-
-
?
S-adenosyl-L-methionine + guanosine18 in tRNATyr
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNATyr
-
involved in post-transcriptional tRNA modification
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNA-Phe
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNA-Phe
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNA-Phe
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNA-Phe
Q9FAC4
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNA-Phe
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNA-Phe
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe
-
-
-
-
?
S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe
Q9FAC4
-
-
-
?
S-adenosyl-L-methionine + tRNA
S-adenosyl-L-homocysteine + 2-methylthio N6(gamma,gamma,dimethylallyl)adenosine in tRNA
-
in vitro
-
?
S-adenosyl-L-methionine + tRNA
S-adenosyl-L-homocysteine + 2-methylthio N6(gamma,gamma,dimethylallyl)adenosine in tRNA
-
in vitro
-
?
S-adenosyl-L-methionine + tRNA
S-adenosyl-L-homocysteine + tRNA containing 2'-O-methylguanine
-
-
-
?
S-adenosyl-L-methionine + tRNA
S-adenosyl-L-homocysteine + tRNA containing 2'-O-methylguanine
-
-
-
-
?
S-adenosyl-L-methionine + tRNA
S-adenosyl-L-homocysteine + tRNA containing 2'-O-methylguanine
Q9FAC4
-
-
-
?
S-adenosyl-L-methionine + tRNA
S-adenosyl-L-homocysteine + tRNA containing 2'-O-methylguanine
-
-
-
-
?
additional information
?
-
recognition region is included within the D-arm structure of tRNA-Leu, a bulge is essentially required. 4 base pairs within the D-stem enhance methyl acceptance activity. Py16Py17G18G19 sequence is important for efficient methyl tranfer
-
-
?
additional information
?
-
-
recognition region is included within the D-arm structure of tRNA-Leu, a bulge is essentially required. 4 base pairs within the D-stem enhance methyl acceptance activity. Py16Py17G18G19 sequence is important for efficient methyl tranfer
-
-
?
additional information
?
-
-
transfers a methyl group to the 2'-hydroxy group of a guanosine residue of a GG-sequence in methyl-deficient tRNATyr from E. coli 58-161
-
-
?
additional information
?
-
-
transfers a methyl group to the 2'-hydroxy group of a guanosine residue of a GG-sequence in methyl-deficient tRNATyr from E. coli 58-161
-
-
?
additional information
?
-
-
specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli
-
-
?
additional information
?
-
-
specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli
-
-
?
additional information
?
-
-
specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli
-
-
?
additional information
?
-
-
specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli
-
-
?
additional information
?
-
-
specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli
-
-
?
additional information
?
-
-
enzyme hydroxylates guanosine at postion 18 in tRNA
-
-
?
additional information
?
-
-
yeast tRNAPhe mutant transcripts are prepared in which the modification site and/or other nucleotides in the D-loop are substituted by dG, inosine, or other nucleotides. Sites of methylation are confirmed with RNA sequencing or primer extension. Although the G18G19 sequence is not essential for methylation by TrmH, disruption of G18G19 severely reduces the efficiency of methyl transfer. There is strict recognition of guanosine by TrmH, in that methylation occurs at the adjacent G19 when the G18 is replaced by dG or adenosine. The fact that TrmH methylates guanosine in D-loops from 4 to 12 nucleotides in length suggests that selection of the position of guanosine within the D-loop is relatively flexible
-
-
?
additional information
?
-
-
specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli
-
-
?
additional information
?
-
-
specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli
-
-
?
additional information
?
-
-
specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli
-
-
?
additional information
?
-
-
specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli
-
-
?
additional information
?
-
-
specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli
-
-
?
additional information
?
-
-
specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.000006
Escherichia coli tRNA-Ile
-
-
-
0.000005
guanosine18 in Escherichia coli tRNA-Met
-
-
-
0.00001
guanosine18 in Escherichia coli tRNA-Phe
-
-
-
0.021 - 0.27
guanosine18 in tRNATyr
-
0.000005 - 0.00001
guanosine18 in yeast tRNA-Phe
-
0.00011 - 0.0013
guanosine18 in yeast tRNAPhe
-
0.000082
guanosine18 in yeast tRNAPhe mutant transcript
-
pH and temperature not specified in the publication, Vmax: 3.1 micromol/mg/h
-
0.0125
guanosine18 in yeast tRNAPhe-AGA mutant transcript
-
pH and temperature not specified in the publication, Vmax: 0.00675 micromol/mg/h
-
0.00082
guanosine18 in yeast tRNAPhe-dG18 mutant transcript
-
pH and temperature not specified in the publication, Vmax: 0.1 micromol/mg/h
-
0.0002
guanosine18 in yeast tRNAPhe-GAA mutant transcript
-
pH and temperature not specified in the publication, Vmax: 0.135 micromol/mg/h
-
0.000245
guanosine18 in yeast tRNAPhe-GGA mutant transcript
-
pH and temperature not specified in the publication, Vmax: 1.2 micromol/mg/h
-
0.0003
guanosine18 in yeast tRNAPhe-GUA mutant transcript
-
pH and temperature not specified in the publication, Vmax: 0.18 micromol/mg/h
-
0.00047 - 28
S-adenosyl-L-methionine
additional information
additional information
-
0.021
guanosine18 in tRNATyr
-
mutant R176A
-
0.022
guanosine18 in tRNATyr
-
mutant H71A
-
0.048
guanosine18 in tRNATyr
-
mutant K90A
-
0.053
guanosine18 in tRNATyr
-
mutant R166A
-
0.065
guanosine18 in tRNATyr
-
mutant R168A
-
0.076
guanosine18 in tRNATyr
-
mutant R109A
-
0.08
guanosine18 in tRNATyr
-
mutant H34A
-
0.087
guanosine18 in tRNATyr
-
mutant L179Stop
-
0.1
guanosine18 in tRNATyr
-
mutant R19A
-
0.12
guanosine18 in tRNATyr
-
mutant K32A
-
0.27
guanosine18 in tRNATyr
-
mutant R8A
-
0.000005
guanosine18 in yeast tRNA-Phe
-
below
-
0.00001
guanosine18 in yeast tRNA-Phe
Q9FAC4
-
-
0.00011
guanosine18 in yeast tRNAPhe
Q9FAC4
mutant E182A, pH not specified in the publication, temperature not specified in the publication
-
0.00012
guanosine18 in yeast tRNAPhe
Q9FAC4
mutant D180A, pH not specified in the publication, temperature not specified in the publication
-
0.00012
guanosine18 in yeast tRNAPhe
Q9FAC4
mutant Q185A, pH not specified in the publication, temperature not specified in the publication
-
0.00014
guanosine18 in yeast tRNAPhe
Q9FAC4
mutant V187A, pH not specified in the publication, temperature not specified in the publication
-
0.00015
guanosine18 in yeast tRNAPhe
Q9FAC4
wild-type, pH not specified in the publication, temperature not specified in the publication
-
0.00016
guanosine18 in yeast tRNAPhe
Q9FAC4
mutant K186A, pH not specified in the publication, temperature not specified in the publication
-
0.00026
guanosine18 in yeast tRNAPhe
Q9FAC4
mutant Y184A, pH not specified in the publication, temperature not specified in the publication
-
0.0013
guanosine18 in yeast tRNAPhe
Q9FAC4
mutant D190A, pH not specified in the publication, temperature not specified in the publication
-
0.00047
S-adenosyl-L-methionine
-
-
0.01
S-adenosyl-L-methionine
-
pH 7.5, 50°C, wild-type
0.01
S-adenosyl-L-methionine
Q9FAC4
pH 7.6, 50°C, mutant I142A
0.01
S-adenosyl-L-methionine
Q9FAC4
pH 7.6, 50°C, wild-type
0.011
S-adenosyl-L-methionine
-
pH 7.5, 50°C, mutant S150C
0.014
S-adenosyl-L-methionine
-
pH 7.5, 50°C, mutant E124D
0.014
S-adenosyl-L-methionine
-
pH 7.5, 50°C, mutant P143Q
0.014
S-adenosyl-L-methionine
Q9FAC4
pH 7.6, 50°C, mutant V128A
0.017
S-adenosyl-L-methionine
Q9FAC4
pH 7.6, 50°C, mutant T99A
0.017
S-adenosyl-L-methionine
-
mutant K32A
0.019
S-adenosyl-L-methionine
-
mutant R19A
0.02
S-adenosyl-L-methionine
-
mutant R168A
0.021
S-adenosyl-L-methionine
-
mutant R166A
0.023
S-adenosyl-L-methionine
-
mutant R109A
0.024
S-adenosyl-L-methionine
-
mutant K90A
0.024
S-adenosyl-L-methionine
-
mutant R176A
0.037
S-adenosyl-L-methionine
-
pH 7.5, 50°C, mutant S150T
0.044
S-adenosyl-L-methionine
-
mutant H71A
0.045
S-adenosyl-L-methionine
-
mutant L179Stop
0.051
S-adenosyl-L-methionine
-
pH 7.5, 50°C, mutant S37G
0.056
S-adenosyl-L-methionine
Q9FAC4
pH 7.6, 50°C, mutant L151A
0.067
S-adenosyl-L-methionine
-
mutant H34A
0.071
S-adenosyl-L-methionine
-
mutant R8A
0.083
S-adenosyl-L-methionine
-
pH 7.5, 50°C, mutant N35A
0.13
S-adenosyl-L-methionine
Q9FAC4
pH 7.6, 50°C, mutant L101A
0.15
S-adenosyl-L-methionine
Q9FAC4
pH 7.6, 50°C, mutant M144A
0.3
S-adenosyl-L-methionine
Q9FAC4
pH 7.6, 50°C, mutant R41K
0.67
S-adenosyl-L-methionine
-
pH 7.5, 50°C, mutant N35D
0.8
S-adenosyl-L-methionine
Q9FAC4
pH 7.6, 50°C, mutant N152A
0.92
S-adenosyl-L-methionine
-
pH 7.5, 50°C, mutant R41M
1.7
S-adenosyl-L-methionine
Q9FAC4
pH 7.6, 50°C, mutant S150A
2.1
S-adenosyl-L-methionine
-
pH 7.5, 50°C, mutant N35Q
28
S-adenosyl-L-methionine
Q9FAC4
pH 7.6, 50°C, mutant E124A
additional information
additional information
-
overview: Km of 28 tRNAPhe variants from yeast
-
additional information
additional information
kinetic parameters of chimeric tRNA transcripts
-
additional information
additional information
-
kinetic parameters of chimeric tRNA transcripts
-
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D180A
Q9FAC4
42% of wild-type activity
D190A
Q9FAC4
5% of wild-type activity
E124D
-
small increase in Km-value
E124Q
-
no enzymic activity
E182A
Q9FAC4
29% of wild-type activity
H34A
-
no change of affinity for S-adenosyl-L-methionine and tRNA
H71A
-
has increased affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
I142A
Q9FAC4
kinetic parameters comparable to wild-type
K186A
Q9FAC4
7% of wild-type activity
K32A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
K90A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
L101A
Q9FAC4
13fold increase in Km-value
L151A
Q9FAC4
5fold increase in Km-value
M144A
Q9FAC4
15fold increase in Km-value
N152D
-
no enzymic activity
N152E
-
no enzymic activity
N35A
-
marked decrease in methyl transfer activity
N35D
-
marked decrease in Km-value for tRNA, low affinity for S-adenosyl-L-homocysteine
N35Q
-
dramatic increase in Km-value
P143Q
-
small increase in Km-value
Q185A
Q9FAC4
16% of wild-type activity
R109A
-
no change of affinity for S-adenosyl-L-methionine and slightly increased affinity for tRNA
R11A
-
has lost its affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R14M
-
dissociation of subunits consistent with that from the wild-type enzyme
R166A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R168A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R176A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R19A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
R41A
Q9FAC4
no enzymic activity
R41K
Q9FAC4
30fold increase in Km-value
R41M
-
large increase in Km-value
R8A
-
no change of affinity for S-adenosyl-L-methionine, decreased affinity for tRNA
S150A
Q9FAC4
large increase in Km-value
S150C
-
Km-value similar to wild-type, decrease in Vmax
S150T
-
4fold increase in Km-value
S37G
-
5fold increase in Km-value
T99A
Q9FAC4
small increase in Km-value
V128A
Q9FAC4
small increase in Km-value
V187A
Q9FAC4
11% of wild-type activity
Y184A
Q9FAC4
10% of wild-type activity
R14M
-
dissociation of subunits consistent with that from the wild-type enzyme
-
E124A
Q9FAC4
13fold increase in Km-value
E124A
-
extensive structural alterations compared to wild-type, marked decrease in activity
L179Stop
Q9FAC4
complete loss of activity
L179Stop
-
no change of affinity for S-adenosyl-L-methionine, markedly decreased affinity for tRNA
N152A
Q9FAC4
80fold increase in Km-value
N152A
-
extensive structural alterations compared to wild-type, marked decrease in activity
additional information
-
spoU gene mutant contains no residue 18, shows no activity but does not influence growth rate
additional information
-
chimeric proteins of Escherichia coli and Thermus thermophilus enzymes demonstrate that the catalytic domain discriminates substrate tRNAs from nonsubstrate tRNAs. The N- and C-terminal regions do not function in the substrate tRNA discrimination process. The C-terminal region works in the initial binding process, in which nonsubstrate tRNA is not excluded, and structural movement of the motif 2 region of the catalytic domain in an induced-fit process is involved in substrate tRNA discrimination
additional information
Q9FAC4
chimeric proteins of Escherichia coli and Thermus thermophilus enzymes demonstrate that the catalytic domain discriminates substrate tRNAs from nonsubstrate tRNAs. The N- and C-terminal regions do not function in the substrate tRNA discrimination process. The C-terminal region works in the initial binding process, in which nonsubstrate tRNA is not excluded, and structural movement of the motif 2 region of the catalytic domain in an induced-fit process is involved in substrate tRNA discrimination
additional information
-
chimeric proteins of Escherichia coli and Thermus thermophilus enzymes demonstrate that the catalytic domain discriminates substrate tRNAs from nonsubstrate tRNAs. The N- and C-terminal regions do not function in the substrate tRNA discrimination process. The C-terminal region works in the initial binding process, in which nonsubstrate tRNA is not excluded, and structural movement of the motif 2 region of the catalytic domain in an induced-fit process is involved in substrate tRNA discrimination
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Gefter, M.L.
The in vitro synthesis of 2-methylguanosine and 2-methylthio 6N(gamma,gamma, dimethylallyl)adenosine in transfer RNA of Escherichia coli
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1982
Thermus thermophilus, Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
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Recognition mechanism of tRNA with tRNA(guanosine-2)methyltransferase from Thermus thermophilus HB 27
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-
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Two types of tRNA(Gm)methylase found in extreme thermophile, Thermus thermophilus strains HB 8 and HB 27
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-
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A thermostable Gm-methylase recognizes the tertiary structure of tRNA
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101
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Thermus thermophilus, Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
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Recognition sites of tRNA by a thermostable tRNA(guanosine-2-)-methyltransferase from Thermus thermophilus HB27
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1990
Thermus thermophilus, Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
brenda
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Substrate recognition of tRNA (guanosine-2'-)-methyltransferase from Thermus thermophilus HB27
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273
25721-25727
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Thermus thermophilus, Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
brenda
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Essentially minimal sequence for substrate recognition by tRNA (guanosine-2')-methyltransferase from Thermus thermophilus HB27
Nucleic Acids Symp. Ser.
37
189-190
1997
Thermus thermophilus, Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
brenda
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Identification and characterization of tRNA (Gm18) methyltransferase from Thermus thermophilus HB8: domain structure and conserved amino acid sequence motifs
Genes Cells
7
259-272
2002
Thermus thermophilus (Q9FAC4), Thermus thermophilus
brenda
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The spoU gene of Escherichia coli, the fourth gene of the spoT operon, is essential for tRNA (Gm18) 2'-O-methyltransferase activity
Nucleic Acids Res.
25
4093-4097
1997
Escherichia coli
brenda
Hori, H.; Kubota, S.; Watanabe, K.; Kim, J.M.; Ogasawara, T.; Sawasaki, T.; Endo, Y.
Aquifex aeolicus tRNA (Gm18) methyltransferase has unique substrate specificity: tRNA recognition mechanism of the enzyme
J. Biol. Chem.
278
25081-25090
2003
Aquifex aeolicus (O67577), Aquifex aeolicus
brenda
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Roles of conserved amino acid sequence motifs in the SpoU (TrmH) RNA methyltransferase family
J. Biol. Chem.
280
10368-10377
2005
Thermus thermophilus
brenda
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Deep knot structure for construction of active site and cofactor binding site of tRNA modification enzyme
Structure
12
593-602
2004
Thermus thermophilus (Q9FAC4)
brenda
Pleshe, E.; Truesdell, J.; Batey, R.T.
Structure of a class II TrmH tRNA-modifying enzyme from Aquifex aeolicus
Acta Crystallogr. Sect. F
61
722-728
2005
Aquifex aeolicus (O67577)
brenda
Watanabe, K.; Nureki, O.; Fukai, S.; Endo, Y.; Hori, H.
Functional categorization of the conserved basic amino acid residues in TrmH (tRNA (Gm18) methyltransferase) enzymes
J. Biol. Chem.
281
34630-34639
2006
Thermus thermophilus
brenda
Watanabe, K.; Nureki, O.; Fukai, S.; Endo, Y.; Hori, H.
Structural change of tRNA (Gm18) methyltransferase by binding of methyl donor analogues
Nucleic Acids Symp. Ser.
49
301-302
2005
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
brenda
Iwashita, C.; Hori, H.
Amount changes of tRNA modification enzymes in Thermus thermophilus HB8 cells according to culture temperatures
Nucleic Acids Symp. Ser.
50
247-248
2006
Thermus thermophilus, Thermus thermophilus HB8 / ATCC 27634 / DSM 579
brenda
Ochi, A.; Makabe, K.; Kuwajima, K.; Hori, H.
Flexible recognition of the tRNA G18 methylation target site by TrmH methyltransferase through first binding and induced fit processes
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285
9018-9029
2010
Thermus thermophilus
brenda
Subramanian, M.; Srinivasan, T.; Sudarsanam, D.
Examining the Gm18 and m(1)G modification positions in tRNA sequences
Genomics Inform.
12
71-75
2014
Nitrobacter hamburgensis, Marinobacter nauticus, Marinomonas sp., Marinomonas sp. MWYL1
brenda
Ochi, A.; Makabe, K.; Yamagami, R.; Hirata, A.; Sakaguchi, R.; Hou, Y.M.; Watanabe, K.; Nureki, O.; Kuwajima, K.; Hori, H.
The catalytic domain of topological knot tRNA methyltransferase (TrmH) discriminates between substrate tRNA and nonsubstrate tRNA via an induced-fit process
J. Biol. Chem.
288
25562-25574
2013
Escherichia coli, Thermus thermophilus (Q9FAC4), Thermus thermophilus
brenda
Gehrig, S.; Eberle, M.E.; Botschen, F.; Rimbach, K.; Eberle, F.; Eigenbrod, T.; Kaiser, S.; Holmes, W.M.; Erdmann, V.A.; Sprinzl, M.; Bec, G.; Keith, G.; Dalpke, A.H.; Helm, M.
Identification of modifications in microbial, native tRNA that suppress immunostimulatory activity
J. Exp. Med.
209
225-233
2012
Escherichia coli
brenda
Joeckel, S.; Nees, G.; Sommer, R.; Zhao, Y.; Cherkasov, D.; Hori, H.; Ehm, G.; Schnare, M.; Nain, M.; Kaufmann, A.; Bauer, S.
The 2-O-methylation status of a single guanosine controls transfer RNA-mediated Toll-like receptor 7 activation or inhibition
J. Exp. Med.
209
235-241
2012
Escherichia coli, Thermus thermophilus
brenda
Hori, H.; Terui, Y.; Nakamoto, C.; Iwashita, C.; Ochi, A.; Watanabe, K.; Oshima, T.
Effects of polyamines from Thermus thermophilus, an extreme-thermophilic eubacterium, on tRNA methylation by tRNA (Gm18) methyltransferase (TrmH)
J. Biochem.
159
509-517
2016
Thermus thermophilus (Q5SM16), Thermus thermophilus, Thermus thermophilus DSM 579 (Q5SM16)
brenda
Freund, I.; Buhl, D.K.; Boutin, S.; Kotter, A.; Pichot, F.; Marchand, V.; Vierbuchen, T.; Heine, H.; Motorin, Y.; Helm, M.; Dalpke, A.H.; Eigenbrod, T.
2-O-methylation within prokaryotic and eukaryotic tRNA inhibits innate immune activation by endosomal Toll-like receptors but does not affect recognition of whole organisms
RNA
25
869-880
2019
Saccharomyces cerevisiae, Escherichia coli, Homo sapiens (Q13395), Homo sapiens, Escherichia coli BW25113
brenda