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The expected taxonomic range for this enzyme is: Corydalis cava
Synonyms
methyltransferase, protoberberine, protoberberine 13-methyltransferase, S-adenosylmethionine:protoberberine-13C-methyltransferase,
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methyltransferase, protoberberine
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protoberberine 13-methyltransferase
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S-adenosylmethionine:protoberberine-13C-methyltransferase
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S-adenosyl-L-methionine + palmatine + 2 NADPH + H+ = S-adenosyl-L-homocysteine + corydaline + 2 NADP+
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methyl group transfer
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S-adenosyl-L-methionine:protoberberine 13-C-methyltransferase
Also acts on 7,8-dihydropalmatine.
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S-adenosyl-L-methionine + berberine + NADPH + H+
S-adenosyl-L-homocysteine + corydaline + NADP+
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S-adenosyl-L-methionine + columbamine + NADPH + H+
S-adenosyl-L-homocysteine + isocorypalmine + NADP+
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27% of the activity with berberine
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S-adenosyl-L-methionine + coptisine + NADPH + H+
S-adenosyl-L-homocysteine + tetrahydrocorysamine + NADP+
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21% of the activity with berberine
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S-adenosyl-L-methionine + dihydroberberine + NADPH + H+
S-adenosyl-L-homocysteine + thalictricavine + NADP+
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93% of the activity with berberine
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S-adenosyl-L-methionine + jatrorrhizine + NADPH + H+
S-adenosyl-L-homocysteine + 7,8-dehydrothalictricavine + NADP+
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67% of the activity with berberine
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S-adenosyl-L-methionine + palmatine + NADPH + H+
S-adenosyl-L-homocysteine + corydaline + NADP+
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93% of the activity with berberine
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S-adenosyl-L-homocysteine
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0.0027
S-adenosyl-L-methionine
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0.0026
S-adenosyl-L-homocysteine
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additional information
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7 - 9.5
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pH 7.0: about 45% of maximal activity, pH 9.5: about 30% of maximal activity
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20 - 50
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20°C: about 35% of maximal activity, 50°C: about 75% of maximal activity
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brenda
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brenda
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brenda
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Rueffer, M.; Bauer, W.; Zenk, M.H.
The formation of corydaline and related alkaloids in Corydalis cava in vivo and in vitro
Can. J. Chem.
72
170-175
1994
Corydalis cava
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brenda
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