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(S)-2-amino-6-oxoheptanedioate + H+ + NADH + NH4+
meso-2,6-diaminoheptanedioate + H2O + NAD+
(S)-2-amino-6-oxoheptanedioate + H+ + NADPH + NH4+
meso-2,6-diaminoheptanedioate + H2O + NADP+
2,6-diaminoheptanedioic acid + H2O + NADP+
2-amino-6-oxopimelate + NH3 + NADPH
-
mixture of all possible stereoisomers, 4% of activity with meso-2,6-diaminopimelate
-
r
2-amino-6-(hydroxyamino)heptanedioic acid + H2O + NADP+
2-hydroxyamino-6-oxopimelate + NH3 + NADPH
-
mixture of all possible stereoisomers, 22% of activity with meso-2,6-diaminopimelate
-
r
2-oxo(phenyl)acetate + NH3 + NADPH + H+
(2S)-amino(phenyl)acetate + NADP+
-
-
-
r
2-oxo-3,3-dimethylbutanoate + NH3 + NADPH + H+
(2S)-2-amino-3,3-dimethylbutanoate + NADP+
-
-
-
r
2-oxo-3-methylbutanoate + NH3 + NADPH + H+
(2S)-2-amino-3-methylbutanoate + NADP+
-
-
-
r
2-oxo-3-phenylpropanoate + NH3 + NADPH + H+
(2S)-2-amino-3-phenylpropanoate + NADP+
-
-
-
r
2-oxo-4-methylpentanoate + NH3 + NADPH + H+
(2S)-2-amino-4-methylpentanoate + NADP+
-
-
-
r
2-oxo-4-phenylbutanoate + NH3 + NADPH + H+
(2S)-2-amino-4-phenylbutanoate + NADP+
-
-
-
r
2-oxobutanoate + NH3 + NADPH + H+
(2S)-2-aminobutanoate + NADP+
-
-
-
r
2-oxobutanoate + NH3 + NADPH + H+
D-2-aminobutanoate + NADP+
2-oxoglutarate + NH3 + NADPH + H+
D-glutamate + NADP+
2-oxooctanoate + NH3 + NADPH + H+
D-2-aminooctanoate + NADP+
2-oxopentanoate + NH3 + NADPH + H+
(2S)-2-aminopentanoate + NADP+
-
-
-
r
2-oxosuccinic acid + NH3 + NADPH + H+
?
2-oxovalerate + NH3 + NADPH + H+
? + H2O + NADP+
3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + NADPH + H+
(2S)-2-amino-3-(1H-indol-3-yl)propanoate + NADP+
-
-
-
r
3-methyl-2-oxobutanoate + NH3 + NADPH + H+
?
3-methyl-2-oxobutanoate + NH3 + NADPH + H+
? + H2O + NADP+
3-methyl-2-oxobutanoate + NH3 + NADPH + H+
D-valine + NADP+
-
-
-
?
3-methyl-2-oxopentanoic acid + NH3 + NADPH + H+
D-isoleucine + NADP+
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADPH + H+
? + H2O + NADP+
4-methyl-2-oxopentanoate + NH3 + NADPH + H+
D-leucine + NADP+
-
-
-
?
4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+
?
4-methylene diaminopimelate + H2O + NADP+
4-methyleneamino-6-oxopimelate + NH3 + NADPH
-
mixture of all possible stereoisomers, 4% of activity with meso-2,6-diaminopimelate
-
r
4-methylthio-2-oxobutanoate + NH3 + NADPH + H+
D-methionine + NADP+
-
-
-
?
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
meso-2,6-diaminoheptanedioate + NADP+
meso-2,6-diaminoheptanedioate + H2O + NAD+
L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
meso-2,6-diaminoheptanedioate + NADP+
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
oxaloacetate + NH3 + NADPH + H+
? + H2O + NADP+
oxaloacetate + NH3 + NADPH + H+
D-aspartate + NADP+
-
-
-
?
phenylpyruvate + NH3 + NADPH + H+
phenylalanine + NADP+
-
-
-
?
pyruvate + NH3 + NADPH + H+
D-alanine + H2O + NADP+
pyruvate + NH3 + NADPH + H+
D-alanine + NADP+
additional information
?
-
(S)-2-amino-6-oxoheptanedioate + H+ + NADH + NH4+
meso-2,6-diaminoheptanedioate + H2O + NAD+
-
-
-
?
(S)-2-amino-6-oxoheptanedioate + H+ + NADH + NH4+
meso-2,6-diaminoheptanedioate + H2O + NAD+
-
-
-
?
(S)-2-amino-6-oxoheptanedioate + H+ + NADPH + NH4+
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
?
(S)-2-amino-6-oxoheptanedioate + H+ + NADPH + NH4+
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
?
2-oxobutanoate + NH3 + NADPH + H+
D-2-aminobutanoate + NADP+
-
-
-
?
2-oxobutanoate + NH3 + NADPH + H+
D-2-aminobutanoate + NADP+
-
-
-
?
2-oxoglutarate + NH3 + NADPH + H+
D-glutamate + NADP+
-
-
-
?
2-oxoglutarate + NH3 + NADPH + H+
D-glutamate + NADP+
-
-
-
?
2-oxooctanoate + NH3 + NADPH + H+
D-2-aminooctanoate + NADP+
-
-
-
?
2-oxooctanoate + NH3 + NADPH + H+
D-2-aminooctanoate + NADP+
-
-
-
?
2-oxosuccinic acid + NH3 + NADPH + H+
?
-
low activity
-
-
?
2-oxosuccinic acid + NH3 + NADPH + H+
?
-
low activity
-
-
?
2-oxovalerate + NH3 + NADPH + H+
? + H2O + NADP+
-
-
-
?
2-oxovalerate + NH3 + NADPH + H+
? + H2O + NADP+
-
-
-
?
3-methyl-2-oxobutanoate + NH3 + NADPH + H+
?
-
-
-
-
?
3-methyl-2-oxobutanoate + NH3 + NADPH + H+
?
-
low activity
-
-
?
3-methyl-2-oxobutanoate + NH3 + NADPH + H+
?
-
low activity
-
-
?
3-methyl-2-oxobutanoate + NH3 + NADPH + H+
? + H2O + NADP+
-
-
-
?
3-methyl-2-oxobutanoate + NH3 + NADPH + H+
? + H2O + NADP+
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADPH + H+
? + H2O + NADP+
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADPH + H+
? + H2O + NADP+
-
-
-
?
4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+
?
-
-
-
?
4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+
?
-
-
-
?
4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+
?
-
-
-
-
?
4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+
?
-
low activity
-
-
?
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
Achromobacter polymorph
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
Achromobacter superficialis
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
Alcaligenes viscolactis
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
Bacterium mycoides
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
diaminopimelate synthesis is thermodynamically favoured
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
Erwinia aroidea
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
Flavobacterium suaveolens
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
Pseudomonas alkanolytica
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
Sarcina subflava
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
bypath of alpha,epsilon diaminopimelic acid pathway
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
involved in lysine biosynthesis
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
involved in lysine biosynthesis
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
meso-2,6-diaminoheptanedioate + NADP+
-
-
-
?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
meso-2,6-diaminoheptanedioate + NADP+
-
-
-
?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
meso-2,6-diaminoheptanedioate + NADP+
-
-
-
?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
meso-2,6-diaminoheptanedioate + NADP+
-
-
-
?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
meso-2,6-diaminoheptanedioate + NADP+
-
-
-
?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
meso-2,6-diaminoheptanedioate + NADP+
-
-
-
?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
meso-2,6-diaminoheptanedioate + NADP+
-
-
-
?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
meso-2,6-diaminoheptanedioate + NADP+
-
-
-
?
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
meso-2,6-diaminoheptanedioate + NADP+
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NAD+
L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NAD+
L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NAD+
L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NAD+
L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NAD+
L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NAD+
L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
highest activity
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
highest activity
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + NADP+
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
-
-
?
meso-2,6-diaminoheptanedioate + NADP+
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
-
-
?
meso-2,6-diaminoheptanedioate + NADP+
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
-
-
?
meso-2,6-diaminoheptanedioate + NADP+
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
-
-
?
meso-2,6-diaminoheptanedioate + NADP+
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
-
-
?
meso-2,6-diaminoheptanedioate + NADP+
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
-
-
?
meso-2,6-diaminoheptanedioate + NADP+
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
-
-
?
meso-2,6-diaminoheptanedioate + NADP+
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
-
-
?
meso-2,6-diaminoheptanedioate + NADP+
L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
-
-
?
oxaloacetate + NH3 + NADPH + H+
? + H2O + NADP+
-
-
-
?
oxaloacetate + NH3 + NADPH + H+
? + H2O + NADP+
-
-
-
?
pyruvate + NADPH
?
-
-
-
?
pyruvate + NADPH
?
-
-
-
?
pyruvate + NADPH
?
-
-
-
-
?
pyruvate + NADPH
?
-
-
-
?
pyruvate + NADPH
?
-
-
-
-
?
pyruvate + NH3 + NADPH + H+
D-alanine + H2O + NADP+
-
-
-
?
pyruvate + NH3 + NADPH + H+
D-alanine + H2O + NADP+
-
-
-
?
pyruvate + NH3 + NADPH + H+
D-alanine + NADP+
-
-
-
-
?
pyruvate + NH3 + NADPH + H+
D-alanine + NADP+
-
-
-
?
pyruvate + NH3 + NADPH + H+
D-alanine + NADP+
-
-
-
-
?
pyruvate + NH3 + NADPH + H+
D-alanine + NADP+
-
-
-
r
pyruvate + NH3 + NADPH + H+
D-alanine + NADP+
-
-
-
?
pyruvate + NH3 + NADPH + H+
D-alanine + NADP+
-
-
-
?
additional information
?
-
no activity with phenylpyruvate
-
-
?
additional information
?
-
no activity with phenylpyruvate
-
-
?
additional information
?
-
-
no activity with phenylpyruvate
-
-
?
additional information
?
-
the enzyme has a more relaxed substrate specificity and potential for D-amino acid synthesis
-
-
?
additional information
?
-
-
the enzyme has a more relaxed substrate specificity and potential for D-amino acid synthesis
-
-
?
additional information
?
-
meso-diaminopimelate dehydrogenase is an NADP-dependent enzyme which catalyzes the reversible oxidative deamination on the D-configuration of meso-2,6-diaminopimelate to produce L-2-amino-6-oxopimelate. The purified enzyme also shows activity toward D-alanine, D-valine, and D-lysine. This enzyme catalyzes the reductive amination of 2-keto acids such as pyruvic acid to generate D-amino acids in up to 99% conversion and 99% enantiomeric excess. Substrate specificities of recombinant wild-type and mutant emzymes in both amination and deamination, overview
-
-
?
additional information
?
-
-
meso-diaminopimelate dehydrogenase is an NADP-dependent enzyme which catalyzes the reversible oxidative deamination on the D-configuration of meso-2,6-diaminopimelate to produce L-2-amino-6-oxopimelate. The purified enzyme also shows activity toward D-alanine, D-valine, and D-lysine. This enzyme catalyzes the reductive amination of 2-keto acids such as pyruvic acid to generate D-amino acids in up to 99% conversion and 99% enantiomeric excess. Substrate specificities of recombinant wild-type and mutant emzymes in both amination and deamination, overview
-
-
?
additional information
?
-
enzyme is able to catalyze reductive amination
-
-
-
additional information
?
-
-
enzyme is able to catalyze reductive amination
-
-
-
additional information
?
-
-
meso-diaminopimelate dehydrogenase catalyzes the NAD(P)-dependent oxidative deamination of meso-diaminopimelate stereoselectively acting on the D-configuration of meso-diaminopimelate. The enzyme is highly selective for meso-diaminopimelate as the electron donor, and NADP+ but not NAD+ can serve as the electron acceptor
-
-
?
additional information
?
-
-
no activity with DL-2-aminopimelate, D-glutamate, L-glutamate, D-aspartate, L-aspartate, D-alanine, L-alanine, D-valine, L-valine, D-lysine, L-lysine, D-phenylalanine, L-phenylalanine, D-leucine, L-leucine, D-threonine, L-threonine, D-serine, L-serine, D-tryptophan, L-tryptophan, D-cysteine, L-cysteine, D-histidine, L-histidine, D-methionine, D-arginine, D-proline, D-asparagine, D-glutamine, D-isoleucine, and D-ornithine, and with NAD+
-
-
?
additional information
?
-
-
meso-diaminopimelate dehydrogenase catalyzes the NAD(P)-dependent oxidative deamination of meso-diaminopimelate stereoselectively acting on the D-configuration of meso-diaminopimelate. The enzyme is highly selective for meso-diaminopimelate as the electron donor, and NADP+ but not NAD+ can serve as the electron acceptor
-
-
?
additional information
?
-
-
no activity with DL-2-aminopimelate, D-glutamate, L-glutamate, D-aspartate, L-aspartate, D-alanine, L-alanine, D-valine, L-valine, D-lysine, L-lysine, D-phenylalanine, L-phenylalanine, D-leucine, L-leucine, D-threonine, L-threonine, D-serine, L-serine, D-tryptophan, L-tryptophan, D-cysteine, L-cysteine, D-histidine, L-histidine, D-methionine, D-arginine, D-proline, D-asparagine, D-glutamine, D-isoleucine, and D-ornithine, and with NAD+
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
additional information
?
-
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
bypath of alpha,epsilon diaminopimelic acid pathway
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
involved in lysine biosynthesis
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
involved in lysine biosynthesis
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
L-2-amino-6-oxoheptanedioate + NH3 + NADPH
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
-
r
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
-
?
meso-2,6-diaminoheptanedioate + H2O + NADP+
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
-
-
r
additional information
?
-
the enzyme has a more relaxed substrate specificity and potential for D-amino acid synthesis
-
-
?
additional information
?
-
-
the enzyme has a more relaxed substrate specificity and potential for D-amino acid synthesis
-
-
?
additional information
?
-
-
meso-diaminopimelate dehydrogenase catalyzes the NAD(P)-dependent oxidative deamination of meso-diaminopimelate stereoselectively acting on the D-configuration of meso-diaminopimelate. The enzyme is highly selective for meso-diaminopimelate as the electron donor, and NADP+ but not NAD+ can serve as the electron acceptor
-
-
?
additional information
?
-
-
meso-diaminopimelate dehydrogenase catalyzes the NAD(P)-dependent oxidative deamination of meso-diaminopimelate stereoselectively acting on the D-configuration of meso-diaminopimelate. The enzyme is highly selective for meso-diaminopimelate as the electron donor, and NADP+ but not NAD+ can serve as the electron acceptor
-
-
?
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46.1
2-oxo-3-methylbutanoate
mutant W121L/H227I, pH 9, 30°C
12.2
2-oxo-3-phenylpropanoate
mutant W121L/H227I, pH 9, 30°C
17.72
2-oxo-4-methylpentanoate
mutant W121L/H227I, pH 9, 30°C
33.83
2-oxo-4-phenylbutanoate
mutant W121L/H227I, pH 9, 30°C
35.12
2-oxobutanoate
mutant W121L/H227I, pH 9, 30°C
23.5
2-oxopentanoate
mutant W121L/H227I, pH 9, 30°C
36.16 - 57.04
2-oxovalerate
97.88 - 119.2
3-methyl-2-oxobutanoate
26.88 - 33.51
4-methyl-2-oxopentanoate
0.21 - 0.28
L-2-amino-6-oxopimelate
0.11 - 0.57
L-DELTA1-tetrahydrodipicolinate
-
0.11 - 16.7
meso-2,6-diaminoheptanedioate
2.5 - 6.25
meso-2,6-diaminopimelate
3.1
meso-diaminopimelate
-
-
29.92 - 31.5
oxaloacetate
additional information
additional information
-
36.16
2-oxovalerate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
57.04
2-oxovalerate
wild-type, pH not specified in the publication, temperature not specified in the publication
97.88
3-methyl-2-oxobutanoate
wild-type, pH not specified in the publication, temperature not specified in the publication
119.2
3-methyl-2-oxobutanoate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
26.88
4-methyl-2-oxopentanoate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
33.51
4-methyl-2-oxopentanoate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.21
L-2-amino-6-oxopimelate
-
-
0.24
L-2-amino-6-oxopimelate
-
-
0.28
L-2-amino-6-oxopimelate
-
-
0.11
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
0.23
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
0.24
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
0.26
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
0.27
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
0.57
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
0.11
meso-2,6-diaminoheptanedioate
pH 10, 30°C
0.18
meso-2,6-diaminoheptanedioate
mutant T70S, pH not specified in the publication, temperature not specified in the publication
0.21
meso-2,6-diaminoheptanedioate
pH 10, 30°C
0.21
meso-2,6-diaminoheptanedioate
mutant K159R, pH not specified in the publication, temperature not specified in the publication
0.27
meso-2,6-diaminoheptanedioate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.27
meso-2,6-diaminoheptanedioate
mutant S90T, pH not specified in the publication, temperature not specified in the publication
0.37
meso-2,6-diaminoheptanedioate
at pH 10.5 and 25°C
0.39
meso-2,6-diaminoheptanedioate
-
mutant enzyme R35S, at pH 9.6 and 25°C
0.41
meso-2,6-diaminoheptanedioate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.41
meso-2,6-diaminoheptanedioate
-
wild type enzyme, at pH 9.6 and 25°C
0.42
meso-2,6-diaminoheptanedioate
-
mutant enzyme R71A, at pH 9.6 and 25°C
0.43
meso-2,6-diaminoheptanedioate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.43
meso-2,6-diaminoheptanedioate
mutant P69G, pH not specified in the publication, temperature not specified in the publication
0.43
meso-2,6-diaminoheptanedioate
mutant R71S, pH not specified in the publication, temperature not specified in the publication
0.52
meso-2,6-diaminoheptanedioate
mutant V68M, pH not specified in the publication, temperature not specified in the publication
0.53
meso-2,6-diaminoheptanedioate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
0.56
meso-2,6-diaminoheptanedioate
mutant R71I, pH not specified in the publication, temperature not specified in the publication
0.59
meso-2,6-diaminoheptanedioate
mutant R71K, pH not specified in the publication, temperature not specified in the publication
0.63
meso-2,6-diaminoheptanedioate
mutant R71Q, pH not specified in the publication, temperature not specified in the publication
1.02
meso-2,6-diaminoheptanedioate
mutant V14L, pH not specified in the publication, temperature not specified in the publication
1.37
meso-2,6-diaminoheptanedioate
mutant R71E, pH not specified in the publication, temperature not specified in the publication
1.37
meso-2,6-diaminoheptanedioate
mutant V156D, pH not specified in the publication, temperature not specified in the publication
1.8
meso-2,6-diaminoheptanedioate
pH 10, 30°C
1.9
meso-2,6-diaminoheptanedioate
pH 10, 30°C
1.9
meso-2,6-diaminoheptanedioate
pH 8.5, 30°C, recombinant wild-type enzyme
2.4
meso-2,6-diaminoheptanedioate
pH 10, 30°C
2.5
meso-2,6-diaminoheptanedioate
pH 8.5, 30°C, recombinant mutant F1446W
2.8
meso-2,6-diaminoheptanedioate
pH 10, 30°C
3.34
meso-2,6-diaminoheptanedioate
-
pH 10.5, temperature not specified in the publication
5.68
meso-2,6-diaminoheptanedioate
pH 10.5, 50°C
15.7
meso-2,6-diaminoheptanedioate
pH 8.5, 30°C, recombinant mutant F146W/M152Q
16.7
meso-2,6-diaminoheptanedioate
pH 8.5, 30°C, recombinant mutant M152Q
2.5
meso-2,6-diaminopimelate
-
-
6.25
meso-2,6-diaminopimelate
-
-
2
NAD+
-
-
890
NAD+
-
pH 10.5, temperature not specified in the publication
0.00083
NADP+
-
-
0.06
NADP+
at pH 10.5 and 25°C
0.302
NADP+
-
pH 10.5, temperature not specified in the publication
0.35
NADP+
-
wild type enzyme, at pH 9.6 and 25°C
0.39
NADP+
-
mutant enzyme R71A, at pH 9.6 and 25°C
0.46
NADP+
-
mutant enzyme R35S, at pH 9.6 and 25°C
0.828
NADP+
pH 10.5, 50°C
0.1
NADPH
-
wild type enzyme, at pH 9.6 and 25°C
0.18
NADPH
-
mutant enzyme R71A, at pH 9.6 and 25°C
0.35
NADPH
-
mutant enzyme R35S, at pH 9.6 and 25°C
12.5
NH3
-
-
4.2
NH4+
pH 8.5, 30°C
29.92
oxaloacetate
wild-type, pH not specified in the publication, temperature not specified in the publication
31.5
oxaloacetate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
11
phenylpyruvate
pH 8.5, 30°C, recombinant mutant T171S/R181F/H227V
11.1
phenylpyruvate
pH 8.5, 30°C, recombinant mutant R181F
12.5
phenylpyruvate
pH 8.5, 30°C, recombinant wild-type enzyme
13.9
phenylpyruvate
pH 8.5, 30°C, recombinant mutant T171S/H227V
13.9
phenylpyruvate
pH 8.5, 30°C, recombinant mutant T171S/R181F
15.5
phenylpyruvate
pH 8.5, 30°C, recombinant mutant H227C
15.8
phenylpyruvate
pH 8.5, 30°C, recombinant mutantT171P
19.6
phenylpyruvate
pH 8.5, 30°C, recombinant mutant T171S
20.8
phenylpyruvate
pH 8.5, 30°C, recombinant mutant R181F/H227V
24.3
phenylpyruvate
pH 8.5, 30°C, recombinant mutant H227V
7.33
pyruvate
mutant V68M, pH not specified in the publication, temperature not specified in the publication
7.45
pyruvate
-
mutant enzyme R35S, at pH 8.6 and 25°C
7.58
pyruvate
mutant K159R, pH not specified in the publication, temperature not specified in the publication
8.86
pyruvate
wild-type, pH not specified in the publication, temperature not specified in the publication
10.08
pyruvate
mutant V14L, pH not specified in the publication, temperature not specified in the publication
10.71
pyruvate
mutant T70S, pH not specified in the publication, temperature not specified in the publication
10.91
pyruvate
mutant R71K, pH not specified in the publication, temperature not specified in the publication
12.2
pyruvate
pH 8.5, 30°C, recombinant mutant F1446W
13.5
pyruvate
pH 8.5, 30°C, recombinant wild-type enzyme
14.08
pyruvate
mutant R71S, pH not specified in the publication, temperature not specified in the publication
16.31
pyruvate
wild-type, pH not specified in the publication, temperature not specified in the publication
16.31
pyruvate
-
wild type enzyme, at pH 8.6 and 25°C
19.17
pyruvate
mutant R71Q, pH not specified in the publication, temperature not specified in the publication
19.24
pyruvate
mutant R71I, pH not specified in the publication, temperature not specified in the publication
20.9
pyruvate
mutant S90T, pH not specified in the publication, temperature not specified in the publication
21.77
pyruvate
-
mutant enzyme R71A, at pH 8.6 and 25°C
23.6
pyruvate
pH 8.5, 30°C, recombinant mutant F146W/M152Q
24.8
pyruvate
pH 8.5, 30°C, recombinant mutant M152Q
25.18
pyruvate
mutant R71E, pH not specified in the publication, temperature not specified in the publication
25.83
pyruvate
mutant P69G, pH not specified in the publication, temperature not specified in the publication
48.46
pyruvate
mutant W121L/H227I, pH 9, 30°C
294.9
pyruvate
wild-type, pH not specified in the publication, temperature not specified in the publication
337.6
pyruvate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.17
2-oxo-3-methylbutanoate
mutant W121L/H227I, pH 9, 30°C
6.2
2-oxo-3-phenylpropanoate
mutant W121L/H227I, pH 9, 30°C
13.49
2-oxo-4-methylpentanoate
mutant W121L/H227I, pH 9, 30°C
12.6
2-oxo-4-phenylbutanoate
mutant W121L/H227I, pH 9, 30°C
35.38
2-oxobutanoate
mutant W121L/H227I, pH 9, 30°C
38.7
2-oxopentanoate
mutant W121L/H227I, pH 9, 30°C
0.91 - 0.93
2-oxovalerate
0.27 - 0.38
3-methyl-2-oxobutanoate
0.1 - 0.12
4-methyl-2-oxopentanoate
4.9 - 278.2
L-DELTA1-tetrahydrodipicolinate
-
1.58 - 127000
meso-2,6-diaminoheptanedioate
0.087 - 0.11
oxaloacetate
0.11 - 3.98
phenylpyruvate
0.91
2-oxovalerate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
0.93
2-oxovalerate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.27
3-methyl-2-oxobutanoate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.38
3-methyl-2-oxobutanoate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
0.1
4-methyl-2-oxopentanoate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
0.12
4-methyl-2-oxopentanoate
wild-type, pH not specified in the publication, temperature not specified in the publication
4.9
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
41.9
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
50.6
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
52.8
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
64.7
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
278.2
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
1.58
meso-2,6-diaminoheptanedioate
mutant V156D, pH not specified in the publication, temperature not specified in the publication
7.7
meso-2,6-diaminoheptanedioate
pH 10, 30°C
8.81
meso-2,6-diaminoheptanedioate
mutant S90T, pH not specified in the publication, temperature not specified in the publication
23.86
meso-2,6-diaminoheptanedioate
mutant T70S, pH not specified in the publication, temperature not specified in the publication
24.5
meso-2,6-diaminoheptanedioate
mutant K159R, pH not specified in the publication, temperature not specified in the publication
29.44
meso-2,6-diaminoheptanedioate
-
mutant enzyme R35S, at pH 9.6 and 25°C
36.05
meso-2,6-diaminoheptanedioate
mutant V68M, pH not specified in the publication, temperature not specified in the publication
36.27
meso-2,6-diaminoheptanedioate
mutant V14L, pH not specified in the publication, temperature not specified in the publication
37.44
meso-2,6-diaminoheptanedioate
mutant R71S, pH not specified in the publication, temperature not specified in the publication
39.8
meso-2,6-diaminoheptanedioate
wild-type, pH not specified in the publication, temperature not specified in the publication
40.46
meso-2,6-diaminoheptanedioate
-
mutant enzyme R71A, at pH 9.6 and 25°C
40.47
meso-2,6-diaminoheptanedioate
wild-type, pH not specified in the publication, temperature not specified in the publication
40.47
meso-2,6-diaminoheptanedioate
-
wild type enzyme, at pH 9.6 and 25°C
45.77
meso-2,6-diaminoheptanedioate
mutant R71Q, pH not specified in the publication, temperature not specified in the publication
48.79
meso-2,6-diaminoheptanedioate
mutant P69G, pH not specified in the publication, temperature not specified in the publication
50.96
meso-2,6-diaminoheptanedioate
mutant R71I, pH not specified in the publication, temperature not specified in the publication
51.99
meso-2,6-diaminoheptanedioate
wild-type, pH not specified in the publication, temperature not specified in the publication
61.58
meso-2,6-diaminoheptanedioate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
65.62
meso-2,6-diaminoheptanedioate
mutant R71E, pH not specified in the publication, temperature not specified in the publication
115.4
meso-2,6-diaminoheptanedioate
pH 10, 30°C
123.3
meso-2,6-diaminoheptanedioate
pH 10, 30°C
145.7
meso-2,6-diaminoheptanedioate
pH 10, 30°C
154.2
meso-2,6-diaminoheptanedioate
pH 10, 30°C
163.6
meso-2,6-diaminoheptanedioate
mutant R71K, pH not specified in the publication, temperature not specified in the publication
344.3
meso-2,6-diaminoheptanedioate
pH 10, 30°C
638
meso-2,6-diaminoheptanedioate
-
pH 10.5, temperature not specified in the publication
737
meso-2,6-diaminoheptanedioate
pH 10.5, 50°C
6780
meso-2,6-diaminoheptanedioate
pH 8.5, 30°C, recombinant mutant F146W/M152Q
15100
meso-2,6-diaminoheptanedioate
pH 8.5, 30°C, recombinant mutant F1446W
35700
meso-2,6-diaminoheptanedioate
pH 8.5, 30°C, recombinant mutant M152Q
127000
meso-2,6-diaminoheptanedioate
pH 8.5, 30°C, recombinant wild-type enzyme
775
NAD+
pH 10.5, 50°C
8933
NAD+
-
pH 10.5, temperature not specified in the publication
17.91
NADP+
-
mutant enzyme R35S, at pH 9.6 and 25°C
25.47
NADP+
-
wild type enzyme, at pH 9.6 and 25°C
58.16
NADP+
-
mutant enzyme R71A, at pH 9.6 and 25°C
792
NADP+
-
pH 10.5, temperature not specified in the publication
0.73
NADPH
pH 8.2, 37°C
2.92
NADPH
-
mutant enzyme R35S, at pH 9.6 and 25°C
4.17
NADPH
-
mutant enzyme R71A, at pH 9.6 and 25°C
6.97
NADPH
-
wild type enzyme, at pH 9.6 and 25°C
160.8
NH4+
pH 8.5, 30°C
0.087
oxaloacetate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.11
oxaloacetate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
0.11
phenylpyruvate
pH 8.5, 30°C, recombinant wild-type enzyme
0.53
phenylpyruvate
pH 8.5, 30°C, recombinant mutantT171P
0.7
phenylpyruvate
pH 8.5, 30°C, recombinant mutant T171S/R181F
0.88
phenylpyruvate
pH 8.5, 30°C, recombinant mutant T171S
1.07
phenylpyruvate
pH 8.5, 30°C, recombinant mutant R181F
2.09
phenylpyruvate
pH 8.5, 30°C, recombinant mutant T171S/R181F/H227V
2.37
phenylpyruvate
pH 8.5, 30°C, recombinant mutant T171S/H227V
2.48
phenylpyruvate
pH 8.5, 30°C, recombinant mutant H227C
3.43
phenylpyruvate
pH 8.5, 30°C, recombinant mutant R181F/H227V
3.98
phenylpyruvate
pH 8.5, 30°C, recombinant mutant H227V
1.35
pyruvate
wild-type, pH not specified in the publication, temperature not specified in the publication
1.63
pyruvate
mutant V14L, pH not specified in the publication, temperature not specified in the publication
1.68
pyruvate
mutant R71E, pH not specified in the publication, temperature not specified in the publication
2.27
pyruvate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
2.96
pyruvate
-
mutant enzyme R35S, at pH 8.6 and 25°C
3.09
pyruvate
mutant R71I, pH not specified in the publication, temperature not specified in the publication
3.31
pyruvate
-
mutant enzyme R71A, at pH 8.6 and 25°C
4.57
pyruvate
mutant P69G, pH not specified in the publication, temperature not specified in the publication
6.15
pyruvate
mutant R71Q, pH not specified in the publication, temperature not specified in the publication
6.34
pyruvate
mutant V68M, pH not specified in the publication, temperature not specified in the publication
6.45
pyruvate
mutant R71K, pH not specified in the publication, temperature not specified in the publication
7
pyruvate
wild-type, pH not specified in the publication, temperature not specified in the publication
7
pyruvate
-
wild type enzyme, at pH 8.6 and 25°C
7.2
pyruvate
mutant R71S, pH not specified in the publication, temperature not specified in the publication
7.61
pyruvate
wild-type, pH not specified in the publication, temperature not specified in the publication
8.07
pyruvate
mutant K159R, pH not specified in the publication, temperature not specified in the publication
8.3
pyruvate
mutant T70S, pH not specified in the publication, temperature not specified in the publication
15.52
pyruvate
mutant S90T, pH not specified in the publication, temperature not specified in the publication
45.22
pyruvate
mutant W121L/H227I, pH 9, 30°C
664
pyruvate
pH 8.5, 30°C, recombinant mutant F146W/M152Q
1010
pyruvate
pH 8.5, 30°C, recombinant mutant M152Q
7850
pyruvate
pH 8.5, 30°C, recombinant wild-type enzyme
10100
pyruvate
pH 8.5, 30°C, recombinant mutant F1446W
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.016 - 1.57
2-oxovalerate
0.0028 - 1.14
3-methyl-2-oxobutanoate
0.0034 - 1.15
4-methyl-2-oxopentanoate
8.6 - 2318.3
L-DELTA1-tetrahydrodipicolinate
-
0.96 - 1638.6
meso-2,6-diaminoheptanedioate
0.0029 - 1.24
oxaloacetate
0.016
2-oxovalerate
wild-type, pH not specified in the publication, temperature not specified in the publication
1.57
2-oxovalerate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
0.0028
3-methyl-2-oxobutanoate
wild-type, pH not specified in the publication, temperature not specified in the publication
1.14
3-methyl-2-oxobutanoate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
0.0034
4-methyl-2-oxopentanoate
wild-type, pH not specified in the publication, temperature not specified in the publication
1.15
4-methyl-2-oxopentanoate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
8.6
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
155
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
194.6
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
220
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
281.3
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
2318.3
L-DELTA1-tetrahydrodipicolinate
pH 8.5, 30°C
-
0.96
meso-2,6-diaminoheptanedioate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
1.15
meso-2,6-diaminoheptanedioate
mutant V156D, pH not specified in the publication, temperature not specified in the publication
13
meso-2,6-diaminoheptanedioate
pH 10.5, 50°C
32.4
meso-2,6-diaminoheptanedioate
mutant S90T, pH not specified in the publication, temperature not specified in the publication
35.5
meso-2,6-diaminoheptanedioate
mutant V14L, pH not specified in the publication, temperature not specified in the publication
41.2
meso-2,6-diaminoheptanedioate
pH 10, 30°C
47.83
meso-2,6-diaminoheptanedioate
mutant R71E, pH not specified in the publication, temperature not specified in the publication
64.3
meso-2,6-diaminoheptanedioate
pH 10, 30°C
64.9
meso-2,6-diaminoheptanedioate
pH 10, 30°C
69.7
meso-2,6-diaminoheptanedioate
mutant V68M, pH not specified in the publication, temperature not specified in the publication
70
meso-2,6-diaminoheptanedioate
pH 10, 30°C
72.68
meso-2,6-diaminoheptanedioate
mutant R71Q, pH not specified in the publication, temperature not specified in the publication
75.07
meso-2,6-diaminoheptanedioate
-
mutant enzyme R35S, at pH 9.6 and 25°C
80.9
meso-2,6-diaminoheptanedioate
pH 10, 30°C
86.73
meso-2,6-diaminoheptanedioate
mutant R71S, pH not specified in the publication, temperature not specified in the publication
90.84
meso-2,6-diaminoheptanedioate
mutant R71I, pH not specified in the publication, temperature not specified in the publication
96.54
meso-2,6-diaminoheptanedioate
-
mutant enzyme R71A, at pH 9.6 and 25°C
98.48
meso-2,6-diaminoheptanedioate
wild-type, pH not specified in the publication, temperature not specified in the publication
98.48
meso-2,6-diaminoheptanedioate
-
wild type enzyme, at pH 9.6 and 25°C
113.3
meso-2,6-diaminoheptanedioate
mutant P69G, pH not specified in the publication, temperature not specified in the publication
117.2
meso-2,6-diaminoheptanedioate
mutant K159R, pH not specified in the publication, temperature not specified in the publication
120.3
meso-2,6-diaminoheptanedioate
wild-type, pH not specified in the publication, temperature not specified in the publication
130
meso-2,6-diaminoheptanedioate
mutant T70S, pH not specified in the publication, temperature not specified in the publication
148.7
meso-2,6-diaminoheptanedioate
wild-type, pH not specified in the publication, temperature not specified in the publication
192
meso-2,6-diaminoheptanedioate
-
pH 10.5, temperature not specified in the publication
279.4
meso-2,6-diaminoheptanedioate
mutant R71K, pH not specified in the publication, temperature not specified in the publication
1638.6
meso-2,6-diaminoheptanedioate
pH 10, 30°C
5.22
NAD+
pH 10.5, 50°C
1003
NAD+
-
pH 10.5, temperature not specified in the publication
39.02
NADP+
-
mutant enzyme R35S, at pH 9.6 and 25°C
73.83
NADP+
-
wild type enzyme, at pH 9.6 and 25°C
100.2
NADP+
pH 10.5, 50°C
148.06
NADP+
-
mutant enzyme R71A, at pH 9.6 and 25°C
2617
NADP+
-
pH 10.5, temperature not specified in the publication
1.8
NADPH
pH 8.2, 37°C
8.32
NADPH
-
mutant enzyme R35S, at pH 9.6 and 25°C
23.49
NADPH
-
mutant enzyme R71A, at pH 9.6 and 25°C
69.29
NADPH
-
wild type enzyme, at pH 9.6 and 25°C
23.8
NH4+
pH 8.5, 30°C
0.0029
oxaloacetate
wild-type, pH not specified in the publication, temperature not specified in the publication
1.24
oxaloacetate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
0.0046
pyruvate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.07
pyruvate
mutant R71E, pH not specified in the publication, temperature not specified in the publication
0.15
pyruvate
-
mutant enzyme R71A, at pH 8.6 and 25°C
0.16
pyruvate
mutant R71I, pH not specified in the publication, temperature not specified in the publication
0.16
pyruvate
mutant V14L, pH not specified in the publication, temperature not specified in the publication
0.18
pyruvate
mutant P69G, pH not specified in the publication, temperature not specified in the publication
0.32
pyruvate
mutant R71Q, pH not specified in the publication, temperature not specified in the publication
0.4
pyruvate
-
mutant enzyme R35S, at pH 8.6 and 25°C
0.43
pyruvate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.43
pyruvate
-
wild type enzyme, at pH 8.6 and 25°C
0.51
pyruvate
mutant R71S, pH not specified in the publication, temperature not specified in the publication
0.59
pyruvate
mutant R71K, pH not specified in the publication, temperature not specified in the publication
0.74
pyruvate
mutant S90T, pH not specified in the publication, temperature not specified in the publication
0.78
pyruvate
mutant T70S, pH not specified in the publication, temperature not specified in the publication
0.86
pyruvate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.86
pyruvate
mutant V68M, pH not specified in the publication, temperature not specified in the publication
1.06
pyruvate
mutant K159R, pH not specified in the publication, temperature not specified in the publication
1.46
pyruvate
mutant A69R, pH not specified in the publication, temperature not specified in the publication
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Q154L/T173I/R199M/P248S/H249N/N276S
F146W
site-directed mutagenesis, the mutant shows altered activity levels with meso-2,6-diaminoheptanedioate and pyruvate as substrates
F146W/M152Q
site-directed mutagenesis, the mutant shows altered activity levels with meso-2,6-diaminoheptanedioate and pyruvate as substrates
H227C
site-directed saturation mutagenesis, the mutant shows 15.1fold increased activity with phenylpyruvate compared to the wild-type enzyme
H227I
mutant accepts substrates D-2-phenylglycine and D-homophenylalanine
K159R
mutation decreases the kcat/KM value with meso-DAP, the catalytic efficiency toward pyruvic acid increases by 24%
M152A
the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme
M152H
the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and no activity towards pyruvate compared to the wild type enzyme
M152K
the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme
M152L
the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and pyruvate compared to the wild type enzyme
M152N
the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme
M152Q
site-directed mutagenesis, the mutant shows altered activity levels with meso-2,6-diaminoheptanedioate and pyruvate as substrates
M152S
the mutant shows increased activity towards meso-2,6-diaminoheptanedioate and reduced activity towards pyruvate compared to the wild type enzyme
P69G
mutation results in decrease of kcat/KM with meso-DAP and pyruvic acid
R181F
site-directed saturation mutagenesis, the mutant shows 6.4fold increased activity with phenylpyruvate compared to the wild-type enzyme
R181F/H227V
site-directed saturation mutagenesis, the mutant shows 19.3fold increased activity with phenylpyruvate compared to the wild-type enzyme
R35S
-
the mutant shows reduced catalytic efficiency with meso-2,6-diaminoheptanedioate and NADP+ compared to the wild type enzyme
R35S/R36V
the mutations significantly lower the specific activity toward NADP+ compared to the wild type enzyme. The mutant enzyme favors NAD+ over NADP+
R71E
for reductive amination, the mutant shows a 1.5fold increase in Km and 0.24fold decrease in kcat. For oxidative deamination, a 50% decrease in the kcat/Km value is observed
R71I
R71I shows 0.37fold decrease in kcat/Km value for pyruvic acid
R71K
the kcat/Km values toward pyruvic acid and meso-DAP increase by 1.4fold and 1.8fold, respectively
R71Q
the kcat/Km values toward pyruvic acid and meso-DAP both decrease by 30%
R71S
for pyruvic acid, R71S shows few changes in kcat/Km values
S90T
mutation results in decrease of kcat/KM with meso-DAP and pyruvic acid
T171H
mutant accepts substrates D-2-phenylglycine and D-homophenylalanine
T171P
site-directed saturation mutagenesis, the mutant shows 2.2fold increased activity with phenylpyruvate compared to the wild-type enzyme
T171S
site-directed saturation mutagenesis, the mutant shows 2.8fold increased activity with phenylpyruvate compared to the wild-type enzyme
T171S/H227V
site-directed saturation mutagenesis, the mutant shows 10.6fold increased activity with phenylpyruvate compared to the wild-type enzyme
T171S/R181F
site-directed saturation mutagenesis, the mutant shows 4.0fold increased activity with phenylpyruvate compared to the wild-type enzyme
T171S/R181F/H227V
site-directed saturation mutagenesis, the mutant shows 14.6fold increased activity with phenylpyruvate compared to the wild-type enzyme
T70S
catalytic ability of T70S does not change much
V14L
mutation results in decrease of kcat/KM with meso-DAP and pyruvic acid
V156D
mutation results in decrease of kcat/KM with meso-DAP and pyruvic acid, almost complete loss of catalytic ability
V68M
mutation decreases the kcat/KM value with meso-DAP, the catalytic efficiency toward pyruvic acid does not change
W121L
mutant accepts substrates D-2-phenylglycine and D-homophenylalanine
W121L/H227I
mutant sow improved enzyme activities towards various 2-oxoacids including sterically bulky substrates. The substrate binding cavity of the mutant enzyme is reshaped to accommodate these bulky substrates, thus leading to higher enzyme activity
R35S
-
the mutant shows reduced catalytic efficiency with meso-2,6-diaminoheptanedioate and NADP+ compared to the wild type enzyme
-
R71A
-
the mutant shows about wild type catalytic efficiency with meso-2,6-diaminoheptanedioate and increased catalytic efficiency with NADP+ compared to the wild type enzyme
-
Q154L/D158G/T173I/R199M/H249N
Q154L/T173I/R199M/P248S/H249N/N276S
the mutant shows strongly reduced deamination activity with meso-2,6-diaminoheptanedioate and increased animation activity with pyruvate, phenylpyruvate, and 4-methyl-2-oxopentanoic acid compared to the wild type enzyme
Q154L/T173I/R199M/P248S/H249N/N276S
-
the mutant shows strongly reduced deamination activity with meso-2,6-diaminoheptanedioate and increased animation activity with pyruvate, phenylpyruvate, and 4-methyl-2-oxopentanoic acid compared to the wild type enzyme
-
A69R
mutation to the correspondung residue of Symbiobacteium thermophilum DAPDH. Mutation improves the catalytic efficiencies toward 2-keto acids and does not affect the catalytic efficiency toward meso-DAP
A69R
-
mutation to the correspondung residue of Symbiobacteium thermophilum DAPDH. Mutation improves the catalytic efficiencies toward 2-keto acids and does not affect the catalytic efficiency toward meso-DAP
-
H227V
site-directed saturation mutagenesis, the mutant shows 35.1fold increased activity with phenylpyruvate compared to the wild-type enzyme
H227V
mutant shows high activity toward phenylpyruvic acid and 2-oxo-4-phenylbutyric acid
R71A
-
the mutant shows about wild type catalytic efficiency with meso-2,6-diaminoheptanedioate and increased catalytic efficiency with NADP+ compared to the wild type enzyme
R71A
mutation destroys the cation-pi interaction between residues R71 and Y205
Q154L/D158G/T173I/R199M/H249N
construction of a thermostable, NADP+-dependent D-amino acid dehydrogenase (DAADH) from the meso-diaminopimelate dehydrogenase of strain A1 by introducing five point mutations into amino acid residues located in the active site. In the presence of NADP+, the mutant enzyme catalyzes the oxidative deamination of several D-amino acids, including D-cyclohexylalanine, D-isoleucine, and D-2-aminooctanoate, but not of meso-diaminopimelate. The corresponding 2-oxo acids are aminated in the presence of NADPH and ammonia in the reverse reaction, mutant substrate specificity, overview. The mutant enzyme is also more thermostable than its parental meso-diaminopimelate dehydrogenase
Q154L/D158G/T173I/R199M/H249N
-
construction of a thermostable, NADP+-dependent D-amino acid dehydrogenase (DAADH) from the meso-diaminopimelate dehydrogenase of strain A1 by introducing five point mutations into amino acid residues located in the active site. In the presence of NADP+, the mutant enzyme catalyzes the oxidative deamination of several D-amino acids, including D-cyclohexylalanine, D-isoleucine, and D-2-aminooctanoate, but not of meso-diaminopimelate. The corresponding 2-oxo acids are aminated in the presence of NADPH and ammonia in the reverse reaction, mutant substrate specificity, overview. The mutant enzyme is also more thermostable than its parental meso-diaminopimelate dehydrogenase
-
additional information
-
systems-wide metabolic pathway engineering in Corynebacterium glutamicum for bio-based production of diaminopentane. Superior strains with desirable properties such as (i) the release from unwanted feedback regulation at the level of aspartokinase and pyruvate carboxylase by introducing the point mutations lysC311 and pycA458, (ii) an optimized supply of the key precursor oxaloacetate by amplifying the anaplerotic enzyme, pyruvate carboxylase, and deleting phosphoenolpyruvate carboxykinase which otherwise removes oxaloacetate, (iii) enhanced biosynthetic flux via combined amplification of aspartokinase, dihydrodipicolinate reductase, diaminopimelate dehydrogenase and diaminopimelate decarboxylase, and (iv) attenuated flux into the threonine pathway competing with production by the leaky mutation hom59 in the homoserine dehydrogenasegene
additional information
in order to enlarge the substrate binding pocket of the meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum to accommodate larger 2-keto acids, e.g. phenylalanine, four amino acid residues, Phe146, Thr171, Arg181, and His227, are targeted for site saturation mutagenesis
additional information
-
in order to enlarge the substrate binding pocket of the meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum to accommodate larger 2-keto acids, e.g. phenylalanine, four amino acid residues, Phe146, Thr171, Arg181, and His227, are targeted for site saturation mutagenesis
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Bartlett, A.T.M.; White, P.J.
Species of Bacillus that make a vegetative peptidoglycan containing lysine lack diaminopimelate epimerase but have diaminopimelate dehydrogenase
J. Gen. Microbiol.
131
2145-2152
1985
Sporosarcina globispora, Paenibacillus macerans, Sporosarcina pasteurii, Lysinibacillus sphaericus
-
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Lam, L.K.P.; Arnold, L.D.; Kalantar, T.H.; Kelland, J.G.; Lane-Bell, P.M.; Palcic, M.M.; Pickard, M.A.; Vederas, J.C.
Analogs of diaminopimelic acid as inhibitors of meso-diaminopimelate dehydrogenase and LL-diaminopimelate epimerase
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263
11814-11819
1988
Lysinibacillus sphaericus
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Abbott, S.D.; Lane-Bell, P.; Sidhu, K.P.S.; Vederas, J.C.
Synthesis and testing of heterocyclic analogues of diaminopimelic acid (DAP) as inhibitors of DAP dehydrogenase and DAP epimerase
J. Am. Chem. Soc.
116
6513-6520
1994
Lysinibacillus sphaericus
-
brenda
Chatterjee, S.P.; Singh, B.K.; Gilvarg, C.
Biosynthesis of lysine in plants: the putative role of meso-diaminopimelate dehydrogenase
Plant Mol. Biol.
26
285-290
1994
no activity in Chlamydomonas reinhardtii, no activity in Glycine max, no activity in Nicotiana tabacum, no activity in Zea mays
brenda
Yeh, P.; Sicard, A.M.; Sinskey, A.J.
General organization of the genes specifically involved in the diaminopimelate-lysine biosynthetic pathway of Corynebacterium glutamicum
Mol. Gen. Genet.
212
105-111
1988
Corynebacterium glutamicum, Corynebacterium glutamicum AS019
brenda
Cremer, J.; Treptow, C.; Eggeling, L.; Sahm, H.
Regulation of enzymes of lysine biosynthesis in Corynebacterium glutamicum
J. Gen. Microbiol.
134
3221-3229
1988
Corynebacterium glutamicum
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Bartlett, A.T.M.; White, P.J.
Regulation of the enzymes of lysine biosynthesis in Bacillus sphaericus NCTC 9602 during vegetative growth
J. Gen. Microbiol.
132
3169-3177
1986
Lysinibacillus sphaericus
-
brenda
Ishino, S.; Mizukami, T.; Yamaguchi, K.; Katsumata, R.; Araki, K.
Cloning and sequencing of the meso-diaminopimelate-D-dehydrogenase (ddh) gene of Corynebacterium glutamicum
Agric. Biol. Chem.
52
2903-2909
1988
Corynebacterium glutamicum, Corynebacterium glutamicum RRL-5
-
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Ishino, S.; Mizukami, T.; Yamaguchi, K.; Katsumata, R.; Araki, K.
Nucleotide sequence of the meso-diaminopimelate D-dehydrogenase gene from Corynebacterium glutamicum
Nucleic Acids Res.
15
3917
1987
Corynebacterium glutamicum
brenda
Misono, H.; Ogasawara, M.; Nagasaki, S.
Characterization of meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum and its distribution in bacteria
Agric. Biol. Chem.
50
2729-2734
1986
Achromobacter polymorph, Achromobacter superficialis, Enterobacter cloacae, Agrobacterium tumefaciens, Alcaligenes viscolactis, Glutamicibacter protophormiae, Terrabacter tumescens, Bacillus amyloliquefaciens, Brevibacillus brevis, Bacillus cereus, Bacillus licheniformis, Lysinibacillus sphaericus, Bacterium mycoides, Corynebacterium ammoniagenes, Corynebacterium glutamicum, Brevibacterium sp., Corynebacterium pseudodiphtheriticum, Hafnia alvei, Flavobacterium suaveolens, Micrococcus luteus, Kocuria rosea, Morganella morganii, no activity in Escherichia coli, no activity in Klebsiella pneumoniae, no activity in Staphylococcus aureus, Providencia rettgeri, Pseudomonas aeruginosa, Pseudomonas alkanolytica, Pseudomonas putida, Pseudomonas chlororaphis subsp. aureofaciens, Pseudomonas cruciviae, Pseudomonas fragi
-
brenda
Misono, H.; Ogasawara, M.; Nagasaki, S.
Purification and properties of meso-diaminopimelate dehydrogenase from Brevibacterium sp.
Agric. Biol. Chem.
50
1329-1330
1986
Brevibacterium sp., Brevibacterium sp. ICR 7000
-
brenda
White, P.J.
2,6-Diaminopimelate
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
8
377-383
1985
Lysinibacillus sphaericus
-
brenda
Ishino, S.; Yamaguchi, K.; Shirahata, K.; Araki, K.
Involvement of meso-alpha,epsilon-diamino-pimelate D-dehydrogenase in lysine biosynthesis in Corynebacterium glutamicum
Agric. Biol. Chem.
48
2257-2260
1984
Corynebacterium glutamicum
-
brenda
White, P.J.
The essential role of diaminopimelate dehydrogenase in the biosynthesis of lysine by Bacillus sphaericus
J. Gen. Microbiol.
129
739-749
1983
Lysinibacillus sphaericus
-
brenda
Misono, H.; Nagasaki, S.; Soda, K.
meso-alpha,epsilon-Diaminopimelate D-dehydrogenase: sulfhydryl group modification
Agric. Biol. Chem.
45
1455-1460
1981
Lysinibacillus sphaericus
-
brenda
Misono, H.; Soda, K.
Properties of meso-alpha,epsilon-diaminopimelate D-dehydrogenase from Bacillus sphaericus
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255
10599-10605
1980
Lysinibacillus sphaericus
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Misono, H.; Soda, K.
Purification and properties of meso-alpha,epsilon-diaminopimelate D-dehydrogenase from Bacillus sphaericus
Agric. Biol. Chem.
44
227-229
1980
Lysinibacillus sphaericus
-
brenda
Misono, H.; Togawa, H.; Soda, K.
meso-alpha,epsilon-Diaminopimelate D-dehydrogenase: distribution and the reaction product
J. Bacteriol.
137
22-27
1979
Lysinibacillus sphaericus, Corynebacterium glutamicum, Brevibacterium sp., Erwinia aroidea, Proteus mirabilis, Proteus vulgaris, Pseudomonas fluorescens, Sarcina subflava
brenda
Misono, H.; Togawa, H.; Yamamoto, T.; Soda, K.
Occurrence of meso-alpha, epsilon-diaminopimelate dehydrogenase in Bacillus sphaericus
Biochem. Biophys. Res. Commun.
72
89-93
1976
Lysinibacillus sphaericus
brenda
Scapin, G.; Reddy, S.G.; Blanchard, J.S.
Three-dimensional structure of meso-diaminopimelic acid dehydrogenase from Corynebacterium glutamicum
Biochemistry
35
13540-13551
1996
Corynebacterium glutamicum (P04964), Corynebacterium glutamicum
brenda
Reddy, S.G.; Scapin, G.; Blanchard, J.S.
Expression, purification, and crystallization of meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum
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25
514-516
1996
Corynebacterium glutamicum
brenda
Wang, F.; Scapin, G.; Blanchard, J.S.; Angeletti, R.H.
Substrate binding and conformational changes of Corynebacterium glutamicum diaminopimelate dehydrogenase revealed by hydrogen/deuterium exchange and electrospray mass spectrometry
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Corynebacterium glutamicum
brenda
Scapin, G.; Cirilli, M.; Reddy, S.G.; Gao, Y.; Vederas, J.C.; Blanchard, J.S.
Substrate and inhibitor binding sites in Corynebacterium glutamicum diaminopimelate dehydrogenase
Biochemistry
37
3278-3285
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Corynebacterium glutamicum (P04964), Corynebacterium glutamicum
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Cirilli, M.; Scapin, G.; Sutherland, A.; Vederas, J.C.; Blanchard, J.S.
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Lysinibacillus sphaericus, Corynebacterium glutamicum
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Corynebacterium glutamicum
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Highly stable meso-diaminopimelate dehydrogenase from an Ureibacillus thermosphaericus strain A1 isolated from a Japanese compost: purification, characterization and sequencing
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1
43
2011
Ureibacillus thermosphaericus, Ureibacillus thermosphaericus A1
brenda
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Symbiobacterium thermophilum (Q67PI3), Symbiobacterium thermophilum
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Symbiobacterium thermophilum (Q67PI3), Symbiobacterium thermophilum
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Creation of a thermostable NADP+-dependent D-amino acid dehydrogenase from Ureibacillus thermosphaericus strain A1 meso-diaminopimelate dehydrogenase by site-directed mutagenesis
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Ureibacillus thermosphaericus (G1UII1), Ureibacillus thermosphaericus, Ureibacillus thermosphaericus A1 (G1UII1)
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Akita, H.; Seto, T.; Ohshima, T.; Sakuraba, H.
Structural insight into the thermostable NADP+-dependent meso-diaminopimelate dehydrogenase from Ureibacillus thermosphaericus
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Ureibacillus thermosphaericus (G1UII1), Ureibacillus thermosphaericus
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Gao, X.; Zhang, Z.; Zhang, Y.; Li, Y.; Zhu, H.; Wang, S.; Li, C.
A newly determined member of the meso-diaminopimelate dehydrogenase family with a broad substrate spectrum
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Symbiobacterium thermophilum, Symbiobacterium thermophilum IAM14863
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Symbiobacterium thermophilum (Q67PI3), Symbiobacterium thermophilum
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Structural analysis reveals the substrate-binding mechanism for the expanded substrate specificity of mutant meso-diaminopimelate dehydrogenase
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16
924-929
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Clostridium tetani (Q890V3), Clostridium tetani E88 (Q890V3), Clostridium tetani E88
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Zhao, L.; Liu, W.; Chen, X.; Wang, M.; Feng, J.; Wu, Q.; Zhu, D.
Effect of residue Y76 on co-enzyme specificity of meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum
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31
1108-1118
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Symbiobacterium thermophilum (Q67PI3), Symbiobacterium thermophilum
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Stone, V.N.; Parikh, H.I.; El-rami, F.; Ge, X.; Chen, W.; Zhang, Y.; Kellogg, G.E.; Xu, P.
Identification of small-molecule inhibitors against meso-2,6-diaminopimelate dehydrogenase from Porphyromonas gingivalis
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10
e0141126
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Porphyromonas gingivalis (Q7MW40), Porphyromonas gingivalis, Porphyromonas gingivalis W83 (Q7MW40)
brenda
Akita, H.; Nakamichi, Y.; Morita, T.; Matsushika, A.
Characterization of an NAD(P)+-dependent meso-diaminopimelate dehydrogenase from Thermosyntropha lipolytica
Biochim. Biophys. Acta
1868
140476
2020
Thermosyntropha lipolytica (A0A1M5M5H3), Thermosyntropha lipolytica, Thermosyntropha lipolytica DSM 11003 (A0A1M5M5H3)
brenda
Ma, H.; Stone, V.N.; Wang, H.; Kellogg, G.E.; Xu, P.; Zhang, Y.
Diaminopimelic acid (DAP) analogs bearing isoxazoline moiety as selective inhibitors against meso-diaminopimelate dehydrogenase (m-Ddh) from Porphyromonas gingivalis
Bioorg. Med. Chem. Lett.
27
3840-3844
2017
Porphyromonas gingivalis (Q7MW40), Porphyromonas gingivalis, Porphyromonas gingivalis W83 (Q7MW40)
brenda
Cheng, X.; Chen, X.; Feng, J.; Wu, Q.; Zhu, D.
Structure-guided engineering of meso-diaminopimelate dehydrogenase for enantioselective reductive amination of sterically bulky 2-keto acids
Catal. Sci. Technol.
8
4994-5002
2018
Symbiobacterium thermophilum (Q67PI3)
-
brenda
Zhang, Y.; Ma, Q.; Dong, M.; Zhang, X.; Chen, Y.; Gao, X.; Song, Y.
Essential role of amino acid position 71 in substrate preference by meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum IAM14863
Enzyme Microb. Technol.
111
57-62
2018
Corynebacterium glutamicum (P04964), Symbiobacterium thermophilum (Q67PI3), Corynebacterium glutamicum ATCC 13032 (P04964)
brenda
Gao, X.; Ma, Q.; Chen, M.; Dong, M.; Pu, Z.; Zhang, X.; Song, Y.
Insight into the highly conserved and differentiated cofactor-binding sites of meso-diaminopimelate dehydrogenase StDAPDH
J. Chem. Inf. Model.
59
2331-2338
2019
Symbiobacterium thermophilum (Q67PI3), Symbiobacterium thermophilum
brenda
Wang, H.; Kellogg, G.E.; Xu, P.; Zhang, Y.
Exploring the binding mechanisms of diaminopimelic acid analogs to meso-diaminopimelate dehydrogenase by molecular modeling
J. Mol. Graph. Model.
83
100-111
2018
Porphyromonas gingivalis (Q7MW40), Porphyromonas gingivalis W83 (Q7MW40)
brenda
Wu, W.; Zhang, Y.; Liu, D.; Chen, Z.
Efficient mining of natural NADH-utilizing dehydrogenases enables systematic cofactor engineering of lysine synthesis pathway of Corynebacterium glutamicum
Metab. Eng.
52
77-86
2019
Pseudothermotoga thermarum (F7YTT4), Pseudothermotoga thermarum, Pseudothermotoga thermarum DSM 5069 (F7YTT4)
brenda
Akita, H.; Nakamichi, Y.; Morita, T.; Matsushika, A.
Identification and functional characterization of NAD(P)+ -dependent meso-diaminopimelate dehydrogenase from Numidum massiliense
MicrobiologyOpen
9
e1059
2020
Numidum massiliense
brenda
Xu, J.Z.; Ruan, H.Z.; Liu, L.M.; Wang, L.P.; Zhang, W.G.
Overexpression of thermostable meso-diaminopimelate dehydrogenase to redirect diaminopimelate pathway for increasing L-lysine production in Escherichia coli
Sci. Rep.
9
2423
2019
Acetivibrio thermocellus (A3DDX7), Ureibacillus thermosphaericus (G1UII1), Corynebacterium glutamicum (P04964), Bacteroides fragilis (Q64PZ8), Symbiobacterium thermophilum (Q67PI3), Lysinibacillus sphaericus (Q9KWR0), Bacteroides fragilis YCH46 (Q64PZ8), Corynebacterium glutamicum ATCC 13032 (P04964), Acetivibrio thermocellus ATCC 27405 (A3DDX7)
brenda