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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
ec 1.14.1.4, hydroxylase, kynurenate, hydroxylase, kynurenate 7,8-, kynurenate 7,8-hydroxylase, kynurenic acid hydroxylase, kynurenic hydroxylase,
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ec 1.14.1.4
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formerly
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hydroxylase, kynurenate
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hydroxylase, kynurenate 7,8-
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kynurenate 7,8-hydroxylase
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kynurenic acid hydroxylase
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kynurenic hydroxylase
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kynurenate + reduced acceptor + O2 = 7,8-dihydro-7,8-dihydroxykynurenate + acceptor
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kynurenate,hydrogen-donor:oxygen oxidoreductase (hydroxylating)
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kynurenate + NADH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NAD+
kynurenate + NADPH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NADP+
kynurenate + NADH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NAD+
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kynurenate + NADH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NAD+
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kynurenate + NADPH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NADP+
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kynurenate + NADPH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NADP+
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kynurenate + NADH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NAD+
kynurenate + NADPH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NADP+
kynurenate + NADH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NAD+
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kynurenate + NADH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NAD+
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kynurenate + NADPH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NADP+
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kynurenate + NADPH + O2
7,8-dihydro-7,8-dihydroxykynurenate + NADP+
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NADH
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iodoacetate
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inhibition reversed by addition of GSH or cysteine
Kynurenate
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competitive inhibition at 0.2 mM
p-chloromercuribenzoate
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inhibition reversed by addition of GSH or cysteine
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ATCC 11299b
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brenda
overview
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brenda
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A0A0P0IVM8_9CAUD
499
0
55601
TrEMBL
other Location (Reliability: 2)
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9
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stable in presence 10 mM cysteine
390584
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60
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5 min, complete inactivation
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2-mercaptoethanol stabilizes activity
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cysteine stabilizes activity
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GSH stabilizes activity
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precipitation of supernatant with ammonium sulfate in the absence of cysteine, complete loss of activity
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thioglycolate stabilizes activity
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-10°C, ammonium sulfate fractions, loss of activity after a few days
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-10°C, supernatant after high speed centrifugation of crude extract, several months, no loss in activity
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Taniuchi, H.; Hayaishi, O.
Studies on the metabolism of kynurenic acid
J. Biol. Chem.
238
283-293
1963
Pseudomonas fluorescens
brenda
Gibson, D.T.
Assay of enzymes of aromatic metabolism
Methods Microbiol.
6A
463-478
1971
Pseudomonas sp.
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brenda
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