Any feedback?
Information on EC 1.14.19.6 - acyl-CoA (9+3)-desaturase
for references in articles please use BRENDA:EC1.14.19.6Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
1.14.19.6 acyl-CoA (9+3)-desaturaseIUBMB Comments
This microsomal enzyme introduces a cis double bond at position 12 of fatty-acyl-CoAs that contain a cis double bond at position 9. When acting on 19:1Delta10 fatty acyl-CoA the enzyme from the pathogenic protozoan Trypanosoma brucei introduces the new double bond at position 13, indicating that the new double bond is introduced three carbons from the existing cis double bond, towards the methyl-end of the fatty acid. Requires cytochrome b5 as the electron donor .
Specify your search results
Word Map
- 1.14.19.6
-
linoleic
-
desaturation
-
oleic
-
polyunsaturated
- synechocystis
-
alpha-linolenic
- nutrition
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
+
2
+
+
2
=
+
2
+
2
Synonyms
delta 12 desaturase, fad-2, delta-12 fatty acid desaturase, le-fad2, ptfad2, delta 12 fatty acid desaturases, ptfad6, klfad2, delta12-fad, egfad2, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acyl-CoA (9+3)-desaturase
-
-
-
-
delta-12 fatty acid desaturase
delta-12 fatty acid desaturase 2
DELTA12 desaturase
DELTA12 FAD
DELTA12 fatty acid desaturase
DELTA12 oleate hydroxylase
-
shows DELTA12 desaturase activities on 16C and 18C monounsaturated fatty acids
DELTA12-acyl-lipid desaturase
DELTA12-desaturase
DELTA12-desaturase system
DELTA12-FAD
DELTA12-fatty acid desaturase
DELTA12des
DELTA12DS
Des12
DesA
egFAD2
FAD2
FAD2-2
fatty acid desaturase-2
KlFAD2
LKFAD15
microsomal delta-12 oleate desaturase
microsomal omega-6 fatty acid desaturase
oleoyl-DELTA12 desaturase
oleoyl-DELTA12/linoleoyl-DELTA3 desaturase
seed-specific delta-12 fatty acid desaturase 2
DELTA12DS
bifunctional enzyme with strong DELTA12 and low DELTA15 desaturase activities
DELTA12DS
bifunctional enzyme with strong DELTA12 and low DELTA15 desaturase activities
-
DELTA12DS
bifunctional enzyme with strong DELTA12 and low DELTA15 desaturase activities
-
DesA
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O
(1)
-
-
-
palmitoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = (9Z,12Z)-hexadeca-9,12-dienoyl-CoA + 2 ferricytochrome b5 + 2 H2O
(2)
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
acyl-CoA,ferrocytochrome b5:oxygen oxidoreductase (12,13 cis-dehydrogenating)
This microsomal enzyme introduces a cis double bond at position 12 of fatty-acyl-CoAs that contain a cis double bond at position 9. When acting on 19:1Delta10 fatty acyl-CoA the enzyme from the pathogenic protozoan Trypanosoma brucei introduces the new double bond at position 13, indicating that the new double bond is introduced three carbons from the existing cis double bond, towards the methyl-end of the fatty acid. Requires cytochrome b5 as the electron donor [4].
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
84628-81-9
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(10Z)-nonadec-10-enoic acid + reduced acceptor + O2
(10Z,13Z)-nonadeca-10,13-dienoic acid + acceptor + H2O
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
5,8,11,14-eicosatetraenoyl-CoA + reduced acceptor + O2
5,8,11,14,17-eicosadecapentaenoyl-CoA + acceptor + H2O
6,9,12-octadecatrienoyl-CoA + reduced acceptor + O2
6,9,12,15-octadecatetraenoyl-CoA + acceptor + H2O
9,12-octadecadienoyl-CoA + reduced acceptor + O2
9,12,15-octadecatrienoyl-CoA + acceptor + H2O
9-hexadecenoyl-CoA + reduced acceptor + O2
9,12-hexadecadienoyl-CoA + acceptor + H2O
low activity
-
-
?
9-octadecenoyl-CoA + reduced acceptor + O2
9,12-octadecadienoyl-CoA + acceptor + H2O
-
-
-
?
acyl-CoA + reduced acceptor + O2
DELTA12-acyl-CoA + acceptor + H2O
-
-
-
?
cis-7-hexadecenoic acid + reduced acceptor + O2
cis,cis-7,10-hexadecadienoic acid + acceptor + H2O
-
5.2% desaturation
-
-
?
cis-9-heptadecenoic acid + reduced acceptor + O2
cis,cis-9,12-heptadecadienoic acid + acceptor + H2O
-
22.3% desaturation
-
-
?
cis-9-hexadecenoic acid + reduced acceptor + O2
cis,cis-9,12-hexadecadienoic acid + acceptor + H2O
cis-9-icosenoic acid + reduced acceptor + O2
cis,cis-11,14-icosadienoic acid + acceptor + H2O
-
4.1% desaturation
-
-
?
cis-9-octadecenoic acid + reduced acceptor + O2
cis,cis-9,12-octadecadienoic acid + acceptor + 2 H2O
eicosenoic acid + reduced acceptor + O2
20:2DELTA11,14 + acceptor + H2O
62% conversion
-
-
?
heptadecyloleic acid + reduced acceptor + O2
C17:2DELTA9,12 + acceptor + H2O
-
-
-
?
myristoleic acid + reduced acceptor + O2
C14:2DELTA9,12 + acceptor + H2O
-
-
-
?
oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O
palmitoleic acid + reduced acceptor + O2
(9Z,12Z)-hexadeca-9,12-dienoic acid + acceptor + H2O
palmitoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
(9Z,12Z)-hexadeca-9,12-dienoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
pentadecyloleic acid + reduced acceptor + O2
C15:2DELTA9,12 + acceptor + H2O + acceptor
-
-
-
?
(10Z)-nonadec-10-enoic acid + reduced acceptor + O2
(10Z,13Z)-nonadeca-10,13-dienoic acid + acceptor + H2O
-
-
-
-
?
(10Z)-nonadec-10-enoic acid + reduced acceptor + O2
(10Z,13Z)-nonadeca-10,13-dienoic acid + acceptor + H2O
-
-
-
-
?
(10Z)-nonadec-10-enoic acid + reduced acceptor + O2
(10Z,13Z)-nonadeca-10,13-dienoic acid + acceptor + H2O
-
-
-
-
?
(Z)-9-tetradecenoic acid + ?
tetradec-9,12-dienoic acid
-
biosynthetic pathway for producing the sex pheromone component (Z,E)-9,12-tetradecadienyl acetate in moths involves a DELTA12 desaturase
-
-
?
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
-
?
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
5,8,11,14-eicosatetraenoyl-CoA + reduced acceptor + O2
5,8,11,14,17-eicosadecapentaenoyl-CoA + acceptor + H2O
-
-
-
?
5,8,11,14-eicosatetraenoyl-CoA + reduced acceptor + O2
5,8,11,14,17-eicosadecapentaenoyl-CoA + acceptor + H2O
-
-
-
?
6,9,12-octadecatrienoyl-CoA + reduced acceptor + O2
6,9,12,15-octadecatetraenoyl-CoA + acceptor + H2O
-
-
-
?
6,9,12-octadecatrienoyl-CoA + reduced acceptor + O2
6,9,12,15-octadecatetraenoyl-CoA + acceptor + H2O
-
-
-
?
9,12-octadecadienoyl-CoA + reduced acceptor + O2
9,12,15-octadecatrienoyl-CoA + acceptor + H2O
high activity
-
-
?
9,12-octadecadienoyl-CoA + reduced acceptor + O2
9,12,15-octadecatrienoyl-CoA + acceptor + H2O
high activity
-
-
?
cis-9-hexadecenoic acid + reduced acceptor + O2
cis,cis-9,12-hexadecadienoic acid + acceptor + H2O
-
14.7% desaturation
-
-
?
cis-9-hexadecenoic acid + reduced acceptor + O2
cis,cis-9,12-hexadecadienoic acid + acceptor + H2O
-
70.3% desaturation
-
-
?
cis-9-octadecenoic acid + reduced acceptor + O2
cis,cis-9,12-octadecadienoic acid + acceptor + 2 H2O
-
PtFAD2 is involved in the biosynthesis of eicosapentaenoic acid
-
-
?
cis-9-octadecenoic acid + reduced acceptor + O2
cis,cis-9,12-octadecadienoic acid + acceptor + 2 H2O
-
20.6% desaturation
-
-
?
cis-9-octadecenoic acid + reduced acceptor + O2
cis,cis-9,12-octadecadienoic acid + acceptor + 2 H2O
-
cis-9-octadecenoic acid is the most efficient substrate for PtFAD2, 50.3% desaturation
-
-
?
oleic acid + ?
linoleic acid + ?
-
DELTA12-desaturase mutant shows delayed spore germination, a twofold reduction in growth, a reduced level of conidiation and complete loss of sclerotial development, compared with the wild-type enzyme
-
-
?
oleic acid + ?
linoleic acid + ?
linoleic acid was detectable when expressing Fm2 in DELTA12 desaturase knockout Yarrowia lipolytica
-
-
?
oleic acid + ?
linoleic acid + ?
expression in Saccharomyces cerevisiae leads to endogenous production of linoleic acid
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
best substrate with 72% conversion
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
best substrate with 23% conversion
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
FAD2 introduces a double bond in position DELTA12 in oleic acid (18:1) to form linoleic acid (18:2 n-6)
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
specific substrate
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
specific substrate
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
best substrate with 63.3% conversion
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + alpha-linolenic acid + acceptor + H2O
-
-
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + alpha-linolenic acid + acceptor + H2O
-
-
-
-
?
oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
oleoyl-CoA + 2 reduced acceptor + O2 + 2 H+
linoleoyl-CoA + 2 acceptor + 2 H2O
-
-
-
?
oleoyl-CoA + 2 reduced acceptor + O2 + 2 H+
linoleoyl-CoA + 2 acceptor + 2 H2O
-
-
-
?
oleoyl-CoA + reduced acceptor + O2
9,12-octadecadienoyl-CoA + acceptor + 2 H2O
-
-
-
-
?
oleoyl-CoA + reduced acceptor + O2
9,12-octadecadienoyl-CoA + acceptor + 2 H2O
-
-
-
?
oleoyl-CoA + reduced acceptor + O2
9,12-octadecadienoyl-CoA + acceptor + 2 H2O
-
-
-
?
oleoyl-CoA + reduced acceptor + O2
octadec-9,11-dienoyl-CoA + acceptor + 2 H2O
-
-
-
-
?
oleoyl-CoA + reduced acceptor + O2
octadec-9,11-dienoyl-CoA + acceptor + 2 H2O
-
the DELTA12 desaturase provides the key step for the cockroach to become nutritionally independent of dietary lipid and to synthesize eicosanoids de novo
-
-
?
palmitoleic acid + reduced acceptor + O2
(9Z,12Z)-hexadeca-9,12-dienoic acid + acceptor + H2O
-
-
-
?
palmitoleic acid + reduced acceptor + O2
(9Z,12Z)-hexadeca-9,12-dienoic acid + acceptor + H2O
43% conversion
-
-
?
palmitoleic acid + reduced acceptor + O2
(9Z,12Z)-hexadeca-9,12-dienoic acid + acceptor + H2O
-
-
-
-
?
palmitoleic acid + reduced acceptor + O2
(9Z,12Z)-hexadeca-9,12-dienoic acid + acceptor + H2O
-
4.1% conversion
-
-
?
palmitoleic acid + reduced acceptor + O2
(9Z,12Z)-hexadeca-9,12-dienoic acid + acceptor + H2O
-
26.7% conversion
-
-
?
stearolic acid + reduced acceptor + O2
trans-12-octadecen-9-ynoic acid + acceptor + H2O
-
4.8% conversion
-
-
?
stearolic acid + reduced acceptor + O2
trans-12-octadecen-9-ynoic acid + acceptor + H2O
-
35% conversion
-
-
?
additional information
?
-
-
oxygen availability alone can regulate de novo DELTA12-desaturase synthesis in Acanthamoeba castellanii and oxygen can limit the activity of preexisting DELTA12-desaturase
-
-
?
additional information
?
-
-
the main transition in fatty acid metabolism of Acanthamoeba castellanii during batch growth appears to be primarily related to a rapid decline in DELTA12-desaturase activity after 24 h. The resultant large growth-dependent changes in the degree of fatty acid unsaturation would be expected to affect the physical state and/or fluidity of membranes, and may be related to many of the distinctive physiological and biochemical characteristics displayed by Acanthamoeba castellanii in different stages of batch growth
-
-
?
additional information
?
-
one of two membrane-bound fatty acid desaturases occurring in Aspergillus nidulans, a processive bifunctional oleoyl-DELTA12/linoleoyl-DELTA3 desaturase, substrate specificity of the recombinant enzyme, overview
-
-
?
additional information
?
-
one of two membrane-bound fatty acid desaturases occurring in Aspergillus nidulans, a processive bifunctional oleoyl-DELTA12/linoleoyl-DELTA3 desaturase, substrate specificity of the recombinant enzyme, overview
-
-
?
additional information
?
-
-
one of two membrane-bound fatty acid desaturases occurring in Aspergillus nidulans, a processive bifunctional oleoyl-DELTA12/linoleoyl-DELTA3 desaturase, substrate specificity of the recombinant enzyme, overview
-
-
?
additional information
?
-
one of two membrane-bound fatty acid desaturases occurring in Aspergillus nidulans, a strictly monofunctional oleoyl-DELTA12 desaturase, substrate specificity of the recombinant enzyme, overview
-
-
?
additional information
?
-
one of two membrane-bound fatty acid desaturases occurring in Aspergillus nidulans, a strictly monofunctional oleoyl-DELTA12 desaturase, substrate specificity of the recombinant enzyme, overview
-
-
?
additional information
?
-
-
one of two membrane-bound fatty acid desaturases occurring in Aspergillus nidulans, a strictly monofunctional oleoyl-DELTA12 desaturase, substrate specificity of the recombinant enzyme, overview
-
-
?
additional information
?
-
one of two membrane-bound fatty acid desaturases occurring in Aspergillus nidulans, a processive bifunctional oleoyl-DELTA12/linoleoyl-DELTA3 desaturase, substrate specificity of the recombinant enzyme, overview
-
-
?
additional information
?
-
one of two membrane-bound fatty acid desaturases occurring in Aspergillus nidulans, a processive bifunctional oleoyl-DELTA12/linoleoyl-DELTA3 desaturase, substrate specificity of the recombinant enzyme, overview
-
-
?
additional information
?
-
one of two membrane-bound fatty acid desaturases occurring in Aspergillus nidulans, a strictly monofunctional oleoyl-DELTA12 desaturase, substrate specificity of the recombinant enzyme, overview
-
-
?
additional information
?
-
one of two membrane-bound fatty acid desaturases occurring in Aspergillus nidulans, a strictly monofunctional oleoyl-DELTA12 desaturase, substrate specificity of the recombinant enzyme, overview
-
-
?
additional information
?
-
the enzyme can also catalyze DELTA15 desaturation
-
-
?
additional information
?
-
recombinantly expressed enzyme CSFAD2A is a DELTA12 desaturase and has DELTAx+3 activity
-
-
?
additional information
?
-
the enzyme is a bifunctional fatty acid desaturase with both high DELTA12 desaturase activity and unusual DELTA15 desaturase activities
-
-
?
additional information
?
-
the enzyme is a bifunctional fatty acid desaturase with both high DELTA12 desaturase activity and unusual DELTA15 desaturase activities
-
-
?
additional information
?
-
the enzyme is a bifunctional fatty acid desaturase with both high DELTA12 desaturase activity and unusual DELTA15 desaturase activities
-
-
?
additional information
?
-
-
the DELTA12-desaturase enzymatic complex shows a preference towards oleoyl-CoA versus elaidoyl-CoA. Study of substrate specificity of the DELTA12 desaturase system is difficult due to the involvement of numerous enzymes. At least two activities are involved: in a first step, acyl CoA synthetase catalyzes the formation of oleoyl-CoA from olic acid and CoA, then oleoyl-CoA is desaturated into linoleoyl-CoA. No desaturation occurs when CoA is absent in the reaction medium
-
-
?
additional information
?
-
-
the DELTA12-desaturase enzymatic complex shows a preference towards oleoyl-CoA versus elaidoyl-CoA. Study of substrate specificity of the DELTA12 desaturase system is difficult due to the involvement of numerous enzymes. At least two activities are involved: in a first step, acyl CoA synthetase catalyzes the formation of oleoyl-CoA from olic acid and CoA, then oleoyl-CoA is desaturated into linoleoyl-CoA. No desaturation occurs when CoA is absent in the reaction medium
-
-
?
additional information
?
-
-
PtFAD6 is involved in the biosynthesis of hexadecantrienic acid
-
-
?
additional information
?
-
-
no activity with cis-13-docosenoic acid
-
-
?
additional information
?
-
-
synergistic effect of high-light and low temperature on cell growth of the DELTA12 fatty acid desaturase mutant
-
-
?
additional information
?
-
-
no activity with myristoleic acid palmitoleic acid, heptadecenoic acid, elaidic acid, linolenic acid, dihomo-gamma-linolenic acid, arachidonic acid, or docosatetraenoic acid
-
-
?
additional information
?
-
-
the enzyme has dual specificities of a DELTA12-fatty acid desaturase and a v+3-fatty acid desaturase
-
-
?
additional information
?
-
-
no activity with myristoleic acid palmitoleic acid, heptadecenoic acid, elaidic acid, linolenic acid, dihomo-gamma-linolenic acid, arachidonic acid, or docosatetraenoic acid
-
-
?
additional information
?
-
-
the enzyme has dual specificities of a DELTA12-fatty acid desaturase and a v+3-fatty acid desaturase
-
-
?
additional information
?
-
-
no activity with myristoleic acid palmitoleic acid, heptadecenoic acid, elaidic acid, linolenic acid, dihomo-gamma-linolenic acid, arachidonic acid, or docosatetraenoic acid
-
-
?
additional information
?
-
-
the enzyme has dual specificities of a DELTA12-fatty acid desaturase and a v+3-fatty acid desaturase
-
-
?
additional information
?
-
gene Ssd12 encodes a DELTA12-FAD, which can convert 16:1 and 18:1 into 16:2 and 18:2 fatty acids, substrate specificity, overview
-
-
?
additional information
?
-
-
gene Ssd12 encodes a DELTA12-FAD, which can convert 16:1 and 18:1 into 16:2 and 18:2 fatty acids, substrate specificity, overview
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(Z)-9-tetradecenoic acid + ?
tetradec-9,12-dienoic acid
-
biosynthetic pathway for producing the sex pheromone component (Z,E)-9,12-tetradecadienyl acetate in moths involves a DELTA12 desaturase
-
-
?
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
cis-9-octadecenoic acid + reduced acceptor + O2
cis,cis-9,12-octadecadienoic acid + acceptor + 2 H2O
-
PtFAD2 is involved in the biosynthesis of eicosapentaenoic acid
-
-
?
eicosenoic acid + reduced acceptor + O2
20:2DELTA11,14 + acceptor + H2O
62% conversion
-
-
?
oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O
oleoyl-CoA + reduced acceptor + O2
octadec-9,11-dienoyl-CoA + acceptor + 2 H2O
-
the DELTA12 desaturase provides the key step for the cockroach to become nutritionally independent of dietary lipid and to synthesize eicosanoids de novo
-
-
?
palmitoleic acid + reduced acceptor + O2
(9Z,12Z)-hexadeca-9,12-dienoic acid + acceptor + H2O
palmitoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
(9Z,12Z)-hexadeca-9,12-dienoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
-
?
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
oleic acid + ?
linoleic acid + ?
-
DELTA12-desaturase mutant shows delayed spore germination, a twofold reduction in growth, a reduced level of conidiation and complete loss of sclerotial development, compared with the wild-type enzyme
-
-
?
oleic acid + ?
linoleic acid + ?
linoleic acid was detectable when expressing Fm2 in DELTA12 desaturase knockout Yarrowia lipolytica
-
-
?
oleic acid + ?
linoleic acid + ?
expression in Saccharomyces cerevisiae leads to endogenous production of linoleic acid
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
best substrate with 72% conversion
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
best substrate with 23% conversion
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
specific substrate
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
specific substrate
-
-
?
oleic acid + reduced acceptor + O2
linoleic acid + acceptor + H2O
-
best substrate with 63.3% conversion
-
-
?
oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
oleoyl-CoA + 2 reduced acceptor + O2 + 2 H+
linoleoyl-CoA + 2 acceptor + 2 H2O
-
-
-
?
oleoyl-CoA + 2 reduced acceptor + O2 + 2 H+
linoleoyl-CoA + 2 acceptor + 2 H2O
-
-
-
?
oleoyl-CoA + reduced acceptor + O2
9,12-octadecadienoyl-CoA + acceptor + 2 H2O
-
-
-
-
?
oleoyl-CoA + reduced acceptor + O2
9,12-octadecadienoyl-CoA + acceptor + 2 H2O
-
-
-
?
oleoyl-CoA + reduced acceptor + O2
9,12-octadecadienoyl-CoA + acceptor + 2 H2O
-
-
-
?
palmitoleic acid + reduced acceptor + O2
(9Z,12Z)-hexadeca-9,12-dienoic acid + acceptor + H2O
43% conversion
-
-
?
palmitoleic acid + reduced acceptor + O2
(9Z,12Z)-hexadeca-9,12-dienoic acid + acceptor + H2O
-
4.1% conversion
-
-
?
palmitoleic acid + reduced acceptor + O2
(9Z,12Z)-hexadeca-9,12-dienoic acid + acceptor + H2O
-
26.7% conversion
-
-
?
stearolic acid + reduced acceptor + O2
trans-12-octadecen-9-ynoic acid + acceptor + H2O
-
4.8% conversion
-
-
?
stearolic acid + reduced acceptor + O2
trans-12-octadecen-9-ynoic acid + acceptor + H2O
-
35% conversion
-
-
?
additional information
?
-
-
oxygen availability alone can regulate de novo DELTA12-desaturase synthesis in Acanthamoeba castellanii and oxygen can limit the activity of preexisting DELTA12-desaturase
-
-
?
additional information
?
-
-
the main transition in fatty acid metabolism of Acanthamoeba castellanii during batch growth appears to be primarily related to a rapid decline in DELTA12-desaturase activity after 24 h. The resultant large growth-dependent changes in the degree of fatty acid unsaturation would be expected to affect the physical state and/or fluidity of membranes, and may be related to many of the distinctive physiological and biochemical characteristics displayed by Acanthamoeba castellanii in different stages of batch growth
-
-
?
additional information
?
-
-
PtFAD6 is involved in the biosynthesis of hexadecantrienic acid
-
-
?
additional information
?
-
-
synergistic effect of high-light and low temperature on cell growth of the DELTA12 fatty acid desaturase mutant
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
N-ethyl-5-phenylisoxazolium 3'-sulfonate
-
DELTA12-desaturase system, enzymatic complex
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
the proportion of linoleic acid in the total fatty acids produced by transformed Saccharomyces cerevisiae increases from 1.1 mol% when grown at 30°C to 2.9 mol% when grown at 15°C
additional information
-
the proportion of linoleic acid in the total fatty acids produced by transformed Saccharomyces cerevisiae increases from 1.1 mol% when grown at 30°C to 2.9 mol% when grown at 15°C
additional information
optimal condition for expressing DELTA12-fatty acid desaturase in Saccharomyces cerevisiae is 3% galactose induction for 24 h at 15°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
functional expression of a DELTA12 fatty acid desaturase gene from Spinacia oleracea in transgenic Sus scrofa
SwissProt
brenda
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
high-level expression of CSFAD2A during embryo development
brenda
4.2fold increase in mRNA level in mature fruiting bodies compared to mycelium
brenda
3.5fold increase in mRNA level in fruiting body primordia and 4.2fold increase in mRNA level in mature fruiting bodies compared to mycelium
brenda
3.5fold increase in mRNA level in fruiting body primordia compared to mycelium
brenda
additional information
highest enzyme expression level in the mesocarp of fruits at 120-140 DAP (i.e. the fourth (of five) period of fruit development), different expression levels in the other three stages with significantly lower levels compared to the fourth stage
brenda
-
highest enzyme expression level in the mesocarp of fruits at 120-140 DAP (i.e. the fourth (of five) period of fruit development), different expression levels in the other three stages with significantly lower levels compared to the fourth stage
-
brenda
seed-specific delta-12 fatty acid desaturase 2
brenda
composition of major fatty acids in Sapium sebiferum seeds, overview
brenda
additional information
quantitative RT-PCR enzyme expression analysis
brenda
additional information
expression of FAD6 increases fourfold at 4°C compared to expression at 25°C
brenda
additional information
expression of FAD6 increases fourfold at 4°C compared to expression at 25°C
brenda
additional information
-
expression of FAD6 increases fourfold at 4°C compared to expression at 25°C
-
brenda
additional information
-
high expression level in bolls between 12 and 16 days after anthesis
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
endomembrane network-like distribution around chloroplasts
brenda
presence of more than three transmembrane helices in the enzyme
brenda
-
presence of more than three transmembrane helices in the enzyme
brenda
-
enzyme activity is increased by up to 10fold during aeration of cultures
-
brenda
-
greatest in microsomal membranes isolated from early-exponential to mid-exponential phase cells, declines by approximately 50% as cultures progress towards stationary phase
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
malfunction
physiological function
additional information
evolution
all members of the CSFAD2 and CSD6 subclasses of microsomal desaturases consist of a single exon. Evolutionary relationship of Cannabis sativa putative desaturases, overview
evolution
in plants, membrane-bound fatty acid desaturases are present in the plastid and the endoplasmic reticulum. The electron donors for plastid desaturases are typically ferredoxins, while endoplasmic reticulum enzymes use cytochrome b5 (Cytb5)
evolution
in plants, membrane-bound fatty acid desaturases are present in the plastid and the endoplasmic reticulum. The electron donors for plastid desaturases are typically ferredoxins, while endoplasmic reticulum enzymes use cytochrome b5 (Cytb5)
evolution
in plants, membrane-bound fatty acid desaturases are present in the plastid and the endoplasmic reticulum. The electron donors for plastid desaturases are typically ferredoxins, while endoplasmic reticulum enzymes use cytochrome b5 (Cytb5)
evolution
in plants, membrane-bound fatty acid desaturases are present in the plastid and the endoplasmic reticulum. The electron donors for plastid desaturases are typically ferredoxins, while endoplasmic reticulum enzymes use cytochrome b5 (Cytb5)
evolution
in plants, membrane-bound fatty acid desaturases are present in the plastid and the endoplasmic reticulum. The electron donors for plastid desaturases are typically ferredoxins, while endoplasmic reticulum enzymes use cytochrome b5 (Cytb5)
evolution
in plants, membrane-bound fatty acid desaturases are present in the plastid and the endoplasmic reticulum. The electron donors for plastid desaturases are typically ferredoxins, while endoplasmic reticulum enzymes use cytochrome b5 (Cytb5)
evolution
in plants, membrane-bound fatty acid desaturases are present in the plastid and the endoplasmic reticulum. The electron donors for plastid desaturases are typically ferredoxins, while endoplasmic reticulum enzymes use cytochrome b5 (Cytb5)
evolution
in plants, membrane-bound fatty acid desaturases are present in the plastid and the endoplasmic reticulum. The electron donors for plastid desaturases are typically ferredoxins, while endoplasmic reticulum enzymes use cytochrome b5 (Cytb5)
evolution
in plants, membrane-bound fatty acid desaturases are present in the plastid and the endoplasmic reticulum. The electron donors for plastid desaturases are typically ferredoxins, while endoplasmic reticulum enzymes use cytochrome b5 (Cytb5)
evolution
the enzyme belongs to the acyl-CoA desaturase family and possesses a fatty acid desaturase domain
malfunction
-
the docosahexaenoic acid content is increased slightly in DELTA12des-disruption mutants
malfunction
-
the docosahexaenoic acid content is increased slightly in DELTA12des-disruption mutants
-
malfunction
-
the docosahexaenoic acid content is increased slightly in DELTA12des-disruption mutants
-
physiological function
fatty acid desaturases, FADs, play an essential role in fatty acid metabolism and the maintenance of biological membranes
physiological function
the DELTA12-fatty acid desaturase gene is absolutely essential for the biosynthesis of linoleic acid as the main substrate for lipid peroxidation
physiological function
-
inserted heterologous desA gene for DELTA12 acyl-lipid desaturase in potato plants results in improved tolerance of transformants to oxidative stress due to the more efficient maintenance of stable cell membrane structure functioning, and this permits prevention of electron jump to oxygen and, as a result, of accelerated reactive oxygen species generation
physiological function
overexpression of the acyl-lipid DELTA12-desaturase in transformed potato plants promotes fatty acid polyunsaturation and presumably averts the accelerated generation of the superoxide anion, thus suppressing lipid peroxidation under low-temperature stress
physiological function
recombinant enzyme expression improves potato plant resistance to Phytophthora infestans infection
physiological function
Solanum tuberosum plants expressing Synechocystis DELTA12-acyl-lipid desaturase experience less severe thermal and oxidative stress upon cooling and can cope with the cold without considerable increase in the enzyme activity
physiological function
the enzyme increases the tolerance to low temperature
physiological function
the expression of DELTA12 acyl-lipid desaturase positively influences stabilization of not only structure but also functioning of chloroplast membranes in Solanum tuberosum, thus preventing a transfer of electrons from the electron transport chain to oxygen and subsequent reactive oxygen species generation at hypothermia
physiological function
Cofad2-2 might be one of the key genes to regulate oil quality in Camellia oleifera seeds
physiological function
in plants, seed-specific delta-12 fatty acid desaturase 2 (FAD2) is responsible for the high content of linoleic acid by inserting a double bond at the delta-12 (omega-6) position of oleic acid. FAD2-2 genes encoding isoforms of the large and functionally diverse FAD2 gene family are detected in developing seeds suggesting their major roles in storage oil desaturation in seed
physiological function
in plants, seed-specific delta-12 fatty acid desaturase 2 (FAD2) is responsible for the high content of linoleic acid by inserting a double bond at the delta-12 (omega-6) position of oleic acid. FAD2-2 genes encoding isoforms of the large and functionally diverse FAD2 gene family are detected in developing seeds suggesting their major roles in storage oil desaturation in seed
physiological function
in plants, seed-specific delta-12 fatty acid desaturase 2 (FAD2) is responsible for the high content of linoleic acid by inserting a double bond at the delta-12 (omega-6) position of oleic acid. FAD2-2 genes encoding isoforms of the large and functionally diverse FAD2 gene family are detected in developing seeds suggesting their major roles in storage oil desaturation in seed
physiological function
in plants, seed-specific delta-12 fatty acid desaturase 2 (FAD2) is responsible for the high content of linoleic acid by inserting a double bond at the delta-12 (omega-6) position of oleic acid. FAD2-2 genes encoding isoforms of the large and functionally diverse FAD2 gene family are detected in developing seeds suggesting their major roles in storage oil desaturation in seed
physiological function
in plants, seed-specific delta-12 fatty acid desaturase 2 (FAD2) is responsible for the high content of linoleic acid by inserting a double bond at the delta-12 (omega-6) position of oleic acid. FAD2-2 genes encoding isoforms of the large and functionally diverse FAD2 gene family are detected in developing seeds suggesting their major roles in storage oil desaturation in seed
physiological function
in plants, seed-specific delta-12 fatty acid desaturase 2 (FAD2) is responsible for the high content of linoleic acid by inserting a double bond at the delta-12 (omega-6) position of oleic acid. FAD2-2 genes encoding isoforms of the large and functionally diverse FAD2 gene family are detected in developing seeds suggesting their major roles in storage oil desaturation in seed
physiological function
in plants, seed-specific delta-12 fatty acid desaturase 2 (FAD2) is responsible for the high content of linoleic acid by inserting a double bond at the delta-12 (omega-6) position of oleic acid. FAD2-2 genes encoding isoforms of the large and functionally diverse FAD2 gene family are detected in developing seeds suggesting their major roles in storage oil desaturation in seed
physiological function
in plants, seed-specific delta-12 fatty acid desaturase 2 (FAD2) is responsible for the high content of linoleic acid by inserting a double bond at the delta-12 (omega-6) position of oleic acid. FAD2-2 genes encoding isoforms of the large and functionally diverse FAD2 gene family are detected in developing seeds suggesting their major roles in storage oil desaturation in seed
physiological function
in plants, seed-specific delta-12 fatty acid desaturase 2 (FAD2) is responsible for the high content of linoleic acid by inserting a double bond at the delta-12 (omega-6) position of oleic acid. FAD2-2 genes encoding isoforms of the large and functionally diverse FAD2 gene family are detected in developing seeds suggesting their major roles in storage oil desaturation in seed
physiological function
the FAD2 enzyme is responsible for the DELTA12 desaturation of oleic acid (18:1DELTA9) to linoleic acid (18:2DELTA9,12)
physiological function
-
fatty acid desaturases, FADs, play an essential role in fatty acid metabolism and the maintenance of biological membranes
-
additional information
-
expression profile of desA gene from Synechocystis sp. PCC6803 and its effect on cell membrane lipid composition and cold tolerance in prokaryotic, Escherichia coli and eukaryotic, Solanum tuberosum cells, overview
additional information
expression profile of desA gene from Synechocystis sp. PCC6803 and its effect on cell membrane lipid composition and cold tolerance in prokaryotic, Escherichia coli and eukaryotic, Solanum tuberosum cells, overview
additional information
-
shifting of the Mucor rouxii culture from anaerobic to aerobic conditions results in an increase of biomass and total fatty acid content. In addition, the levels of unsaturated fatty acids are enhanced accompanied by a decrease in the levels of medium- and long-chain saturated fatty acids, levels of expressions of the DELTA9-, DELTA12- and DELTA6-desaturases genes are coordinately upregulated after the shift, overview
additional information
the enzyme belongs to the plastidial D12-FAD with conserved histidine boxes
additional information
the enzyme belongs to the plastidial D12-FAD with conserved histidine boxes
additional information
the enzyme contains three histidine-rich domains and four membrane-spanning domains characteristic for DELTA12-fatty acid desaturases
additional information
hemp seeds contain seven DELTA12 desaturases, FAD2A is the major DELAT12 desaturase in hemp seed
additional information
-
the enzyme belongs to the plastidial D12-FAD with conserved histidine boxes
-
Show AA Sequence and Transmembrane Helices (188 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
40500
x * 40500, DesA, SDS-PAGE, x * 66000, about, recombinant DesA-LicBM3 hybrid protein, SDS-PAGE
43000
66000
43000
SDS-PAGE of cell membranes of transformed Escherichia coli
66000
x * 40500, DesA, SDS-PAGE, x * 66000, about, recombinant DesA-LicBM3 hybrid protein, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
?
x * 40500, DesA, SDS-PAGE, x * 66000, about, recombinant DesA-LicBM3 hybrid protein, SDS-PAGE
additional information
enzyme motifs and domain organization, overview
additional information
enzyme motifs and domain organization, overview
additional information
enzyme motifs and domain organization, overview
additional information
enzyme motifs and domain organization, overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
the seed oil fatty acid composition of Arabidopsis plants expressing An1 is altered, overview
additional information
the seed oil fatty acid composition of Arabidopsis plants expressing An1 is altered, overview
additional information
-
the seed oil fatty acid composition of Arabidopsis plants expressing An1 is altered, overview
additional information
-
the seed oil fatty acid composition of Arabidopsis plants expressing An1 is altered, overview
-
additional information
following four backcrosses and sibling crosses to achieve homozygosity, csfad2a-1 is grown in the field and produces a 70 molar per cent high oleic acid (18:1DELTA9) oil at yields similar to wild-type. Cold-pressed high oleic oil produces fewer volatiles and has a sevenfold increase in shelf life compared to wild-type. Two low abundance octadecadienoic acids, 18:2DELTA6,9 and 18:2DELTA9,15, are identified in the high oleic oil, and their presence suggests remaining endogenous desaturase activities utilize the increased levels of oleic acid as substrate. Dramatic fatty acid level changes observed in csfad2a-1 seed confirm that the predicted truncated CSFAD2A protein is nonfunctional. A second allele, csfad2a-2, which carries two point mutations giving rise to a proline to leucine transition at positions 218 and 375 of the predicted amino acid sequence of CSFAD2A. Homozygous csfad2a-2 (BC1F1) seed accumulate nearly 70 molar per cent of oleic acid, low levels of 18:2DELTA6,9 and 18:2DELTA9,15 and decreased levels of linoleic acid and alpha-linolenic acid compared to heterozygous and segregating wild-type seeds from the same population. This seed oil phenotype is very similar to that of csfad2a-1 and is consistent with one or both of the P to L transitions disrupting protein function
additional information
a recombinant Saccharomyces cerevisiae strain EH1315 expressing gene Cop-odeA accumulates four additional fatty acids identified as 9,12-hexadecadienoic acid, 9,12,15-hexadecatrienoic acid, linoleic acid, and alinolenic acid, which comprised 8.8%, 1.0%, 29.0%, and 0.6% of the total fatty acids, respectively, overview
additional information
-
a recombinant Saccharomyces cerevisiae strain EH1315 expressing gene Cop-odeA accumulates four additional fatty acids identified as 9,12-hexadecadienoic acid, 9,12,15-hexadecatrienoic acid, linoleic acid, and alinolenic acid, which comprised 8.8%, 1.0%, 29.0%, and 0.6% of the total fatty acids, respectively, overview
-
additional information
-
a recombinant Saccharomyces cerevisiae strain EH1315 expressing gene Cop-odeA accumulates four additional fatty acids identified as 9,12-hexadecadienoic acid, 9,12,15-hexadecatrienoic acid, linoleic acid, and alinolenic acid, which comprised 8.8%, 1.0%, 29.0%, and 0.6% of the total fatty acids, respectively, overview
-
additional information
for improvment of the linoleic acid content in the lipids of Rhodosporidium toruloides, galactose-inducible expression of the gene encoding DELTA12-fatty acid desaturase (FADS) from Mortierella alpina or Fusarium verticillioides by integration of the corresponding expression cassettes into the genome of Rhodosporidium toruloides haploid and diploid strains
additional information
-
a transgenic Saccharomyces cerevisiae strain BYdesa, expressing the enzyme from Helvea brasiliensis, produces up to 15% polyunsaturated fatty acids, mostly 9Z,12Z-C18:2, linoleic acid, but also 9Z,12Z-C16:2 fatty acid under inducing conditions, production of 4-hydroxy-2-nonenal, one of the major end products of n-6 polyunsaturated fatty acid peroxidation. Desaturase expression causes adaptation to oxidative stress but not to hyperosmotic stress, phenotype, overview
additional information
the DELTA12 desaturase-defective mutant, Mut48, derived from Mortierella alpina 1S-4 produces several fatty acids of the n-9 family such as 6,9-octadecadienoic acid (18:2n-9), 8,11-eicosadienoic acid (20:2n-9), and mead acid. The mutants SR88 and TM912 exhibit a complete DELTA12 desaturation deficiency with no arachidonic acid accumulation
additional information
-
for improvment of the linoleic acid content in the lipids of Rhodosporidium toruloides, galactose-inducible expression of the gene encoding DELTA12-fatty acid desaturase (FADS) from Mortierella alpina or Fusarium verticillioides by integration of the corresponding expression cassettes into the genome of Rhodosporidium toruloides haploid and diploid strains
additional information
-
the DELTA12 desaturase-defective mutant, Mut48, derived from Mortierella alpina 1S-4 produces several fatty acids of the n-9 family such as 6,9-octadecadienoic acid (18:2n-9), 8,11-eicosadienoic acid (20:2n-9), and mead acid. The mutants SR88 and TM912 exhibit a complete DELTA12 desaturation deficiency with no arachidonic acid accumulation
-
additional information
-
construction of a hybrid of desA and reporter gene encoding thermostable lichenase, licBM3. The desaturase can enhance tolerance to cold stress in potato, and desaturase and lichenase retain their functionality in the structure of the hybrid protein where the enzymatic activity of target gene product is higher than in the case of reporter lichenase gene absence in the construction, phenotypes, overview
additional information
construction of a hybrid of desA and reporter gene encoding thermostable lichenase, licBM3. The desaturase can enhance tolerance to cold stress in potato, and desaturase and lichenase retain their functionality in the structure of the hybrid protein where the enzymatic activity of target gene product is higher than in the case of reporter lichenase gene absence in the construction, phenotypes, overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
soluble recombinant enzyme from Escherichia coli strains JM109 and BL21(DE3)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, high level expression in Escherichia coli strains JM109 and BL21(DE3)
-
expressed in Escherichia coli strains XL1Blue and BL21 and Solanum tuberosum cells
expressed in Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae and in Arabidopsis thaliana
expressed in Saccharomyces cerevisiae and in the Arabidopsis thaliana fad2-1 mutant (knockout mutants lacking the single FAD2 gene)
expressed in Saccharomyces cerevisiae EH1315, 7.9% oleic acid and 29% linoleic acid in yeast expressing DELTA12 desaturase compared with 37% oleic acid and no detectable linoleic acid in control yeast, fatty acid composition analyzed by gas-liquid chromatography
expressed in Saccharomyces cerevisiae INVSc1 cells and Aurantiochytrium limacinum mh0186 cells
-
expressed in Saccharomyces cerevisiae INVScI and Pichia pastoris GS115 cells
-
expressed in Solanum tuberosum cultivar Desnitsa
expressed in wild-type Yarrowia lipolytica and its DELTA12 desaturase knockout mutant, 62.6 weight percent of total fatty acid was linoleic acid produced in the mutant
expression in Saccharomyces cerevisiae leads to endogenous production of linoleic acid, the proportion of linoleic acid in the total fatty acids produced by transformed Saccharomyces cerevisiae increases from 1.1 mol% when grown at 30°C to 2.9 mol% when grown at 15°C
expression in Saccharomyces cerevisiae strain YHU3046-4A results in endogenous production of linoleic acid, increasement in production of linoleic acid from 0.66 to 1.19 microg/mg dry cell weight when 0.5 microM oleic acid as a substrate was exogenously added
-
expression of DELTA12-fatty acid desaturase genes in Escherichia coli strain BL21 and Saccharomyces cerevisiae strain IN-VSc1 leads to production of an active enzyme which converts 17.876% and 17.604% of oleic acid to linoleic acid, GC-MS detection in vitro and in vivo
functional expression of a DELTA12 fatty acid desaturase gene from Spinacia oleracea in transgenic Sus scrofa. Levels of linoleic acid (18:2n-6) in adipocytes that have differentiated in vitro from cells derived from the transgenic pigs are about 10times higher than those from wild-type pigs. In addition, the white adipose tissue of transgenic pigs contained about 20% more linoleic acid (18:2n-6) than that of wild-type pigs
gene An1, DNA and amino acid sequence determination and analysis, functional expression in Arabidopsis thaliana
gene An2, DNA and amino acid sequence determination and analysis, functional expression in Arabidopsis thaliana
gene Cofad2-2, DNA and amino acid sequence determination and analysis, sequence comparisons
gene Cop-odeA, DNA and amino acid sequence determination and analysis, functional expression in Saccharomyces cerevisiae strain EH1315
gene Cs-fad2, DNA and amino acid sequence determination and analysis, transcriptional analysis, and functional expression in and complementation of enzyme-deficient Saccharomyces cerevisiae S288C mutant strain NBRC1136
gene desA, recombinant expression of the desA-licBM3 hybrid in transgenic Escherichia coli strains XL1Blue and BL21 and Solanum tuberosum plants
gene egFAD2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR enzyme expression analysis, functional recombinant expression in Saccharomyces cerevisiae strain INVSc1 leading to production of linoleic acids (18:2DELTA9,12), not normally present in wild-type yeast cells, evaluation of fatty acids profiles of the transformed yeast cells by GC/MS analysis, overview
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
gene FAD2A, quantitative RT-PCR enzyme expression analysis, CSFAD2A is cloned into the expression vector pESCTRP containing the galactose-inducible GAL1 promoter, recombinant expression in Saccharomyces cerevisiae
gene FAD6, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression in Saccharomyces cerevisiae strain INVSc1, expression analysis
gene Pc-fad2, DNA and amino acid sequence determination and analysis
gene Ssd12, DNA and amino acid sequence determination and analysis, two genomic copies, expression in Saccharomyces cerevisiae
heterologous expression in Saccharomyces cerevisiae and Aspergillus oryzae
heterologous expression in yeast Saccharomyces cerevisiae and Synechococcus
-
recombinant expression in Rhodosporidium toruloides with integration into the genome using Agrobacterium tumefaciens strain AGL1, the recombinnat expression leads to increased production of linoleic acid from oleic acid in the tranformed cells, while no significant decrease in the relative contents of the other fatty acids, namely, C14:0 (myristic acid), C16:0 (palmitic acid), and C16:1 (palmitoleic acid) is observed
recombinant expression of His6-tagged enzyme FADS1 in Rhodosporidium toruloides with integration into the genome using Agrobacterium tumefaciens strain AGL1, the recombinnat expression leads to increased production of linoleic acid from oleic acid in the tranformed cells, while no significant decrease in the relative contents of the other fatty acids, namely, C14:0 (myristic acid), C16:0 (palmitic acid), and C16:1 (palmitoleic acid) is observed
-
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
gene FAD2, DNA and amino acid sequence deterination and analysis, encoded motifs in comparison, sequence comparisons and phylogenetic analysis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of FAD6 increases fourfold at 4C compared to expression at 25C. FAD6 transcription is sensitive to saline stress with a 20fold increase at 6% NaCl
FAD2 mRNA accumulates in leaf mesophyll cells and in root epidermis cells when exposed to 15°C for 4 days in dark conditions
induction of FAD2 expression following a 24 h incubation at 10°C is light dependent, no difference in FAD2 expression is found in plants grown between 32°C and 20°C
mRNA accumulation shows a periodic behaviour, decreasing to the 22°C levels following a 48 h incubation and re-establishing ist 24 h induction after 96 h
no enhancement of enzyme transcript is observed when cells are incubated in the dark at 22°C
shifting of the Mucor rouxii culture from anaerobic to aerobic conditions upregulates levels of expressions of the DELTA9-, DELTA12- and DELTA6-desaturases genes, overview. The transcriptional response is rapid and transient, with the maximal mRNA levels between 0.5 h and 1.0 h after the shift
-
the induction of FAD2 is inhibited by incubation at 10°C under dark conditions but appears unaffected by light intensity
the level of the enzyme transcript increases 10fold within 1 h upon a decrease in temperature from 36°C to 22°C
expression of FAD6 increases fourfold at 4C compared to expression at 25C. FAD6 transcription is sensitive to saline stress with a 20fold increase at 6% NaCl
expression of FAD6 increases fourfold at 4C compared to expression at 25C. FAD6 transcription is sensitive to saline stress with a 20fold increase at 6% NaCl
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
nutrition
after functional expression of a DELTA12 fatty acid desaturase gene from Spinacia oleracea in transgenic Sus scrofa levels of linoleic acid (18:2n-6) in adipocytes that have differentiated in vitro from cells derived from the transgenic pigs are about 10 times higher than those from wild-type pigs. In addition, the white adipose tissue of transgenic pigs contained about 20% more linoleic acid (18:2n-6) than that of wild-type pigs. These results demonstrate the functional expression of a plant gene for a fatty acid desaturase in mammals, opening up the possibility of modifying the fatty acid composition of products from domestic animals by transgenic technology, using plant genes for fatty acid desaturases
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wilson, R.A.; Calvo, A.M.; Chang, P.K.; Keller, N.P.
Characterization of the Aspergillus parasiticus DELTA12-desaturase gene: A role for lipid metabolism in the Aspergillus-seed interaction
Microbiology
150
2881-2888
2004
Aspergillus parasiticus
brenda
Peyou-Ndi, M.M.; Watts, J.L.; Browse, J.
Identification and characterization of an animal DELTA12 fatty acid desaturase gene by heterologous expression in Saccharomyces cerevisiae
Arch. Biochem. Biophys.
376
399-408
2000
Caenorhabditis elegans
brenda
Borgeson, C.E.; De Renobales, M.; Blomquist, G.J.
Characterization of the DELTA12 desaturase in the American cockroach, Periplaneta americana: the nature of the substrate
Biochim. Biophys. Acta
1047
135-140
1990
Periplaneta americana
brenda
Jurenka, R.A.
Biosynthetic pathway for producing the sex pheromone component (Z,E)-9,12-tetradecadienyl acetate in moths involves a DELTA12 desaturase
Cell. Mol. Life Sci.
53
501-505
1997
Cadra cautella
brenda
Sakuradani, E.; Kobayashi, M.; Ashikari, T.; Shimizu, S.
Identification of DELTA12-fatty acid desaturase from arachidonic acid-producing Mortierella fungus by heterologous expression in the yeast Saccharomyces cerevisiae and the fungus Aspergillus oryzae
Eur. J. Biochem.
261
812-820
1999
Mortierella alpina (Q9Y8H5), Mortierella alpina 1S-4 (Q9Y8H5)
brenda
Borgeson, C.E.; Blomquist, G.J.
Subcellular location of the DELTA12-desaturase rules out bacteriocyte contribution to linoleate biosynthesis in the house cricket and the American cockroach
Insect Biochem. Mol. Biol.
23
297-302
1993
Periplaneta americana, Acheta domesticus
-
brenda
Avery, S.V.; Lloyd, D.; Harwood, J.L.
Changes in membrane fatty acid composition and DELTA12-desaturase activity during growth of Acanthamoeba castellanii in batch culture
J. Eukaryot. Microbiol.
41
396-401
1994
Acanthamoeba castellanii
-
brenda
Nugier-Chauvin, C.; Fauconnot, L.; Daligault, F.; Patin, H.
Enantioselective oxidation of thiafatty acids by an algal DELTA12-desaturase
J. Mol. Catal. B
11
1007-1012
2001
Chlorella vulgaris
-
brenda
Lomascolo, A.; Dubreucq, E.; Galzy, P.
Study of the DELTA12-desaturase system of Lipomyces starkeyi
Lipids
31
253-259
1996
Lipomyces starkeyi, Lipomyces starkeyi CBS 1807
brenda
Avery, S.V.; Rutter, A.J.; Harwood, J.L.; Lloyd, D.
Oxygen-dependent low-temperature DELTA12 (n6)-desaturase induction and alteration of fatty acid composition in Acanthamoeba castellanii
Microbiology
142
2213-2221
1996
Acanthamoeba castellanii
-
brenda
Sakamoto, T.; Bryant, D.A.
Synergistic effect of high-light and low temperature on cell growth of the DELTA12 fatty acid desaturase mutant in Synechococcus sp. PCC 7002
Photosynth. Res.
72
231-242
2002
Synechococcus sp. PCC 7002
brenda
Galle, A.M.; Oursel, A.; Joseph, M.; Kader, J.C.
Solubilization of membrane bound DELTA12- and DELTA6-fatty acid desaturases from borage seeds
Phytochemistry
45
1587-1590
1997
Borago officinalis
-
brenda
Domergue, F.; Spiekermann, P.; Lerchl, J.; Beckmann, C.; Kilian, O.; Kroth, P.G.; Boland, W.; Zahringer, U.; Heinz, E.
New insight into Phaeodactylum tricornutum fatty acid metabolism. Cloning and functional characterization of plastidial and microsomal DELTA12-fatty acid desaturases
Plant Physiol.
131
1648-1660
2003
Phaeodactylum tricornutum
brenda
Saeki, K.; Matsumoto, K.; Kinoshita, M.; Suzuki, I.; Tasaka, Y.; Kano, K.; Taguchi, Y.; Mikami, K.; Hirabayashi, M.; Kashiwazaki, N.; Hosoi, Y.; Murata, N.; Iritani, A.
Functional expression of a DELTA12 fatty acid desaturase gene from spinach in transgenic pigs
Proc. Natl. Acad. Sci. USA
101
6361-6366
2004
Spinacia oleracea (Q8H943)
brenda
Fofana, B.; Cloutier, S.; Duguid, S.; Ching, J.; Rampitsch, C.
Gene expression of stearoyl-ACP desaturase and delta12 fatty acid desaturase 2 is modulated during seed development of flax (Linum usitatissimum)
Lipids
41
705-712
2006
Linum usitatissimum
brenda
Zhang, S.; Sakuradani, E.; Ito, K.; Shimizu, S.
Identification of a novel bifunctional DELTA12/DELTA15 fatty acid desaturase from a basidiomycete, Coprinus cinereus TD#822-2
FEBS Lett.
581
315-319
2007
Coprinopsis cinerea (A2A1C4), Coprinopsis cinerea TD#822-2 (A2A1C4), Coprinopsis cinerea TD822-2 (A2A1C4)
brenda
Sakai, H.; Kajiwara, S.
Cloning and functional characterization of a DELTA12 fatty acid desaturase gene from the basidiomycete Lentinula edodes
Mol. Genet. Genomics
273
336-341
2005
Lentinula edodes (Q65YX3), Lentinula edodes
brenda
Damude, H.G.; Zhang, H.; Farrall, L.; Ripp, K.G.; Tomb, J.F.; Hollerbach, D.; Yadav, N.S.
Identification of bifunctional delta12/omega3 fatty acid desaturases for improving the ratio of omega3 to omega6 fatty acids in microbes and plants
Proc. Natl. Acad. Sci. USA
103
9446-9451
2006
Fusarium verticillioides (Q27ZJ7)
brenda
Li, M.C.; Li, H.; Wei, D.S.; Xing, L.J.
Cloning and molecular characterization of DELTA12-fatty acid desaturase gene from Mortierella isabellina
World J. Gastroenterol.
12
3373-3379
2006
Umbelopsis isabellina (P59668)
brenda
Kainou, K.; Kamisaka, Y.; Kimura, K.; Uemura, H.
Isolation of DELTA12 and omega3-fatty acid desaturase genes from the yeast Kluyveromyces lactis and their heterologous expression to produce linoleic and alpha-linolenic acids in Saccharomyces cerevisiae
Yeast
23
605-612
2006
Kluyveromyces lactis, Kluyveromyces lactis NK1
brenda
Cipak, A.; Jaganjac, M.; Tehlivets, O.; Kohlwein, S.D.; Zarkovic, N.
Adaptation to oxidative stress induced by polyunsaturated fatty acids in yeast
Biochim. Biophys. Acta
1781
283-287
2008
Hevea brasiliensis
brenda
Niu, B.; Ye, H.; Xu, Y.; Wang, S.; Chen, P.; Peng, S.; Ou, Y.; Tang, L.; Chen, F.
Cloning and characterization of a novel DELTA12-fatty acid desaturase gene from the tree Sapium sebiferum
Biotechnol. Lett.
29
959-964
2007
Triadica sebifera (A5J295), Triadica sebifera
brenda
Hoffmann, M.; Hornung, E.; Busch, S.; Kassner, N.; Ternes, P.; Braus, G.H.; Feussner, I.
A small membrane-peripheral region close to the active center determines regioselectivity of membrane-bound fatty acid desaturases from Aspergillus nidulans
J. Biol. Chem.
282
26666-26674
2007
Aspergillus nidulans (Q5AWX6), Aspergillus nidulans (Q5BEJ3), Aspergillus nidulans, Aspergillus nidulans FGSC A4 (Q5AWX6), Aspergillus nidulans FGSC A4 (Q5BEJ3)
brenda
Kargiotidou, A.; Deli, D.; Galanopoulou, D.; Tsaftaris, A.; Farmaki, T.
Low temperature and light regulate delta 12 fatty acid desaturases (FAD2) at a transcriptional level in cotton (Gossypium hirsutum)
J. Exp. Bot.
59
2043-2056
2008
Gossypium hirsutum (Q8W2B9), Gossypium hirsutum
brenda
Yin, D.; Cui, D.; Jia, B.
Construction of a high-efficient expression vector of Delta12 fatty acid desaturase in peanut and its prokaryotical expression
J. Genet. Genomics
34
81-88
2007
Arachis hypogaea
brenda
Meesapyodsuk, D.; Qiu, X.
An oleate hydroxylase from the fungus Claviceps purpurea: cloning, functional analysis, and expression in Arabidopsis
Plant Physiol.
147
1325-1333
2008
Claviceps purpurea
brenda
Oura, T.; Kajiwara, S.
Substrate specificity and regioselectivity of DELTA12 and omega3 fatty acid desaturases from Saccharomyces kluyveri
Biosci. Biotechnol. Biochem.
72
3174-3179
2008
Lachancea kluyveri
brenda
Zhou, X.R.; Horne, I.; Damcevski, K.; Haritos, V.; Green, A.; Singh, S.
Isolation and functional characterization of two independently-evolved fatty acid DELTA12-desaturase genes from insects
Insect Mol. Biol.
17
667-676
2008
Acheta domesticus (B7SB91)
brenda
Sakuradani, E.; Abe, T.; Matsumura, K.; Tomi, A.; Shimizu, S.
Identification of mutation sites on DELTA12 desaturase genes from Mortierella alpina 1S-4 mutants
J. Biosci. Bioeng.
107
99-101
2009
Mortierella alpina (Q9Y8H5), Mortierella alpina 1S-4 (Q9Y8H5)
brenda
Zhang, D.; Pirtle, I.L.; Park, S.J.; Nampaisansuk, M.; Neogi, P.; Wanjie, S.W.; Pirtle, R.M.; Chapman, K.D.
Identification and expression of a new delta-12 fatty acid desaturase (FAD2-4) gene in upland cotton and its functional expression in yeast and Arabidopsis thaliana plants
Plant Physiol. Biochem.
47
462-471
2009
Gossypium hirsutum (B0FYE4), Gossypium hirsutum
brenda
Chen, Q.; Liu, Q.; Wu, Z.; Wang, Z.; Gou, K.
Generation of fad2 transgenic mice that produce omega-6 fatty acids
Sci. China C Life Sci.
52
1048-1054
2009
Gossypium hirsutum
brenda
Yazawa, H.; Iwahashi, H.; Kamisaka, Y.; Kimura, K.; Uemura, H.
Production of polyunsaturated fatty acids in yeast Saccharomyces cerevisiae and its relation to alkaline pH tolerance
Yeast
26
167-184
2009
Kluyveromyces lactis
brenda
Amiri, R.M.; Yureva, N.O.; Shimshilashvili, K.R.; Goldenkova-Pavlova, I.V.; Pchelkin, V.P.; Kuznitsova, E.I.; Tsydendambaev, V.D.; Trunova, T.I.; Los, D.A.; Jouzani, G.S.; Nosov, A.M.
Expression of acyl-lipid Delta12-desaturase gene in prokaryotic and eukaryotic cells and its effect on cold stress tolerance of potato
J. Integr. Plant Biol.
52
289-297
2010
Synechocystis sp., Synechocystis sp. (P20388)
brenda
Ruenwai, R.; Cheevadhanarak, S.; Rachdawong, S.; Tanticharoen, M.; Laoteng, K.
Oxygen-induced expression of DELTA6-, DELTA9- and DELTA12-desaturase genes modulates fatty acid composition in Mucor rouxii
Appl. Microbiol. Biotechnol.
86
327-334
2010
Amylomyces rouxii
brenda
Watanabe, T.; Tsuda, S.; Nishimura, H.; Honda, Y.; Watanabe, T.
Characterization of a DELTA12-fatty acid desaturase gene from Ceriporiopsis subvermispora, a selective lignin-degrading fungus
Appl. Microbiol. Biotechnol.
87
215-224
2010
Phanerodontia chrysosporium (D4Q8H2), Gelatoporia subvermispora (D4Q8S6)
brenda
Lu, Y.; Chi, X.; Li, Z.; Yang, Q.; Li, F.; Liu, S.; Gan, Q.; Qin, S.
Isolation and characterization of a stress-dependent plastidial DELTA12 fatty acid desaturase from the antarctic microalga Chlorella vulgaris NJ-7
Lipids
45
179-187
2010
Chlorella vulgaris (D3U658), Chlorella vulgaris (E2CT06), Chlorella vulgaris NJ-7 (D3U658), Chlorella vulgaris NJ-7 (E2CT06)
brenda
Yan, Z.; Zhuo, L.; Mulan, J.; Xia, W.; Yangmin, G.; Yinbo, Z.; Fenghong, H.
Clone and identification of bifunctional DELTA12/DELTA15 fatty acid desaturase LKFAD15 from Lipomyces kononenkoae
Food Sci. Biotechnol.
22
573-576
2013
Lipomyces kononenkoae, Lipomyces kononenkoae 1714
-
brenda
Matsuda, T.; Sakaguchi, K.; Kobayashi, T.; Abe, E.; Kurano, N.; Sato, A.; Okita, Y.; Sugimoto, S.; Hama, Y.; Hayashi, M.; Okino, N.; Ito, M.
Molecular cloning of a Pinguiochrysis pyriformis oleate-specific microsomal DELTA12-fatty acid desaturase and functional analysis in yeasts and thraustochytrids
J. Biochem.
150
375-383
2011
Pinguiochrysis pyriformis, Pinguiochrysis pyriformis MBIC 10872
brenda
Zhou, X.; Green, A.; Singh, S.
Caenorhabditis elegans ?12-desaturase FAT-2 is a bifunctional desaturase able to desaturate a diverse range of fatty acid substrates at the DELTA12 and DELTA15 positions
J. Biol. Chem.
286
43644-43650
2011
Caenorhabditis elegans (G5EGA5)
brenda
Matsuda, T.; Sakaguchi, K.; Hamaguchi, R.; Kobayashi, T.; Abe, E.; Hama, Y.; Hayashi, M.; Honda, D.; Okita, Y.; Sugimoto, S.; Okino, N.; Ito, M.
Analysis of DELTA12-fatty acid desaturase function revealed that two distinct pathways are active for the synthesis of PUFAs in T. aureum ATCC 34304
J. Lipid Res.
53
1210-1222
2012
Thraustochytrium aureum, Thraustochytrium aureum ATCC 34304
brenda
Shi, J.; Cao, Y.; Fan, X.; Li, M.; Wang, Y.; Ming, F.
A rice microsomal delta-12 fatty acid desaturase can enhance resistance to cold stress in yeast and Oryza sativa
Mol. Breed.
29
743-757
2012
Oryza sativa (Q6ZGW6)
-
brenda
Demin, I.; Naraikina, N.; Tsydendambaev, V.; Moshkov, I.; Trunova, T.
The effect of potato plant transformation with the gene encoding DELTA12-acyl-lipid desaturase on the CO2 exchange and activities of antioxidant enzymes under hypothermia
Russ. J. Plant Physiol.
60
367-374
2013
Synechocystis sp.
-
brenda
Demin I, D.I.; Shimshilashvili C, S.C.; YurEva N, Y.N.; Naraykina N, N.N.; Goldenkova-Pavlova I, G.I.; Los D, L.D.; Nosov A, N.A.; Trunova T, T.T.
Overexpression of the acyl-lipid DELTA12-desaturase gene protects potato plants from low temperature damage
Acta Agron. Hung.
59
87-99
2011
Synechocystis sp. (P20388)
-
brenda
Los D.; Horvath I.; Vigh L.; Murata N.
The temperature-dependent expression of the desaturase gene desA in Synechocystis PCC6803
FEBS Lett.
318
57-60
1993
Synechocystis sp. (P20388)
brenda
Avelange-Macherel M.-H.; Macherel D.; Wada H.; Murata N.
Site-directed mutagenesis of histidine residues in the DELTA12 acyl-lipid desaturase of Synechocystis
FEBS Lett.
361
111-114
1995
Synechocystis sp. (P20388), Synechocystis sp.
brenda
Okada, S.; Zhou, X.R.; Damcevski, K.; Gibb, N.; Wood, C.; Hamberg, M.; Haritos, V.S.
Diversity of DELTA12 fatty acid desaturases in santalaceae and their role in production of seed oil acetylenic fatty acids
J. Biol. Chem.
288
32405-32413
2013
Exocarpos cupressiformis, Santalum acuminatum
brenda
Wada, H.; Gombos, Z.; Murata, N.
Enhancement of chilling tolerance of a cyanobacterium by genetic manipulation of fatty acid desaturation
Nature
347
200-203
1990
Synechocystis sp. (P20388)
brenda
Bielecka, M.; Kaminski, F.; Adams, I.; Poulson, H.; Sloan, R.; Li, Y.; Larson, T.R.; Winzer, T.; Graham, I.A.
Targeted mutation of DELTA12 and DELTA15 desaturase genes in hemp produce major alterations in seed fatty acid composition including a high oleic hemp oil
Plant Biotechnol. J.
12
613-623
2014
Cannabis sativa (A0A088MFF4)
brenda
Sakamoto, T.; Wada, H.; Nishida, I.; Ohmori, M.; Murata, N.
Identification of conserved domains in the DELTA12 desaturases of cyanobacteria
Plant Mol. Biol.
24
643-650
1994
Synechocystis sp. (P20388), Synechococcus sp. (Q55231)
brenda
Demin, I.; Deryabin, A.; Sinkevich, M.; Trunova, T.
Insertion of cyanobacterial desA gene coding for DELTA12-acyl-lipid desaturase increases potato plant resistance to oxidative stress induced by hypothermia
Russ. J. Plant Physiol.
55
639-648
2008
Synechocystis sp. (P20388)
-
brenda
Astakhova, N.V.; Demin, I.N.; Naraikina, N.V.; Trunova, T.I.
Effect of the desA gene encoding DELTA12 acyl-lipid desaturase on the chloroplast structure and tolerance to hypothermia of potato plants
Russ. J. Plant Physiol.
58
18-23
2011
Synechocystis sp. (P20388)
-
brenda
Yureva, N.O.; Kirsanova, S.N.; Kukushkina, L.N.; Pchelkin, V.P.; Sobolkova, G.I.; Nikiforova, K.R.; Goldenkova-Pavlova, I.V.; Nosov, A.M.; Tsydendambaev, V.D.
Expression of the gene encoding DELTA12 acyl-lipid desaturase from Synechocystis sp. PCC 6803 improves potato plant resistance to late blight infection
Russ. J. Plant Physiol.
61
672-678
2014
Synechocystis sp. (P20388)
-
brenda
Gerasymenko, I.M.; Sakhno, L.A.; Kyrpa, T.N.; Ostapchuk, A.M.; Hadjiev, T.A.; Goldenkova-Pavlova, I.V.; Sheludko, Y.V.
Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial DELTA9 or DELTA12 acyl-lipid desaturases and thermostable lichenase
Russ. J. Plant Physiol.
62
283-291
2015
Synechocystis sp. (P20388)
-
brenda
Wang, Y.; Zhang, S.; Poetter, M.; Sun, W.; Li, L.; Yang, X.; Jiao, X.; Zhao, Z.K.
Overexpression of DELTA12-fatty acid desaturase in the oleaginous yeast Rhodosporidium toruloides for production of linoleic acid-rich lipids
Appl. Biochem. Biotechnol.
180
1497-1507
2016
Mortierella alpina, Fusarium verticillioides (Q27ZJ7)
brenda
Lin, P.; Zhou, C.; Yao, X.; Cao, Y.
Sequence and expression characterization of two genes encoding DELTA-12 fatty acid desaturases from Camellia oleifera
Forest Res.
29
743-751
2016
Camellia oleifera (A0A088NBK4)
-
brenda
Sun, R.; Gao, L.; Yu, X.; Zheng, Y.; Li, D.; Wang, X.
Identification of a DELTA12 fatty acid desaturase from oil palm (Elaeis guineensis Jacq.) involved in the biosynthesis of linoleic acid by heterologous expression in Saccharomyces cerevisiae
Gene
591
21-26
2016
Elaeis guineensis (A0A1B0YC62)
brenda
Dehghan Nayeri, F.; Yarizade, K.
Bioinformatics study of delta-12 fatty acid desaturase 2 (FAD2) gene in oilseeds
Mol. Biol. Rep.
41
5077-5087
2014
Arachis hypogaea (E6Y9B0), Brassica napus (C3W519), Carthamus tinctorius (E9M2L7), Cucurbita pepo (Q6QUM6), Glycine max (P48631), Olea europaea (Q4QWZ0), Olea europaea subsp. europaea (F2YFG1), Vernicia fordii (Q8GZC3), Vitis labrusca (F8TVX9)
brenda
Bielecka, M.; Kaminski, F.; Adams, I.; Poulson, H.; Sloan, R.; Li, Y.; Larson, T.R.; Winzer, T.; Graham, I.A.
Targeted mutation of DELTA12 and DELTA15 desaturase genes in hemp produce major alterations in seed fatty acid composition including a high oleic hemp oil
Plant Biotechnol. J.
12
613-623
2014
Cannabis sativa (A0A088MFF4)
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 1.14.19.6)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
