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a dihomo-gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a stearidonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
an arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
arachidonic acid + 2 ferrocytochrome b5 + O2 + 2 H+
eicosapentaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
eicosapentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
omega-6 linoleic acid + 2 ferrocytochrome b5 + O2 + 2 H+
linolenic acid + 2 ferricytochrome b5 + 2 H2O
-
best substrate
-
-
?
additional information
?
-
a dihomo-gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] i.e. dihomo-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
-
-
?
a dihomo-gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a (8Z,11Z,14Z,17Z)-icosa-8,11,14,17-tetraenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid] i.e. dihomo-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
-
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a stearidonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(6Z,9Z,12Z)-octadeca-6,9,12-trienoyl-[glycerolipid] i.e. gamma-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
(6Z,9Z,12Z,15Z)-octadeca-6,9,12,15-tetraenoyl-[glycerolipid] i.e.stearidonoyl-[glycerolipid]
-
?
a gamma-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a stearidonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(6Z,9Z,12Z)-octadeca-6,9,12-trienoyl-[glycerolipid] i.e. gamma-linolenoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
(6Z,9Z,12Z,15Z)-octadeca-6,9,12,15-tetraenoyl-[glycerolipid] i.e.stearidonoyl-[glycerolipid]
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
-
-
-
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
(9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid] i.e. linoleoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
(9Z,12Z,15Z)-octadeca-9,12,15-trienoyl-[glycerolipid] i.e. linolenoyl-[glycerolipid]
-
?
a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
an alpha-linolenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O
(9Z,12Z)-octadeca-9,12-dienoyl-[glycerolipid] i.e. linoleoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
(9Z,12Z,15Z)-octadeca-9,12,15-trienoyl-[glycerolipid] i.e. linolenoyl-[glycerolipid]
-
?
an arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoyl-[glycerolipid] i.e. arachidonoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
-
-
?
an arachidonoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
a (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoyl-[glycerolipid] i.e. arachidonoyl-[glycerolipid]. Yeast transformant is grown in complete medium containing 0.1% of exogenous fatty acids and then the total fatty acid composition is analyzed by GLC. The enzyme accepts electrons from the microsomal cytochrome b5 or cytochrome b5 motif of another desaturases
-
-
?
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+
alpha-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
additional information
?
-
the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity
-
-
?
additional information
?
-
-
the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity
-
-
?
additional information
?
-
the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity
-
-
?
additional information
?
-
-
the enzyme shows no DELTA12-desaturase, DELTA6-desaturase or DELTA5-desaturase activity
-
-
?
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malfunction
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accumulation of linoleoyl (18:2) groups at the expense of linolenoyl groups (18:3) is the only substantial difference in membrane lipid biosynthesis in leaves and roots of fad3-2 plants relative to wild type
metabolism
regulation of the enzyme through synergistic and antagonistic hormonal interactions, overview
metabolism
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regulation of the enzyme through synergistic and antagonistic hormonal interactions, overview
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physiological function
OMEGA-3 fatty acid desaturase converts exogenous arachidonic acid, an OMEGA-6 PUFA, into eicosapentaenoic acid (EPA). Gene sdd17 from EPA-rich fungus is expressed at high levels and increases OMEGA-3 fatty acid concentrations in mammalian cells
physiological function
the omega-3 FAD gene expression product and its enzymatic product C18:3n3 are crucial to the microalgae cell system
physiological function
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tomato endoplasmic reticulum-type omega-3 fatty acid desaturase functions in early seedling tolerance to salinity stress
physiological function
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the omega-3 FAD gene expression product and its enzymatic product C18:3n3 are crucial to the microalgae cell system
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additional information
fatty acid profile of wild-type and fad3 mutant roots in response to several plant hormones, overview
additional information
-
fatty acid profile of wild-type and fad3 mutant roots in response to several plant hormones, overview
additional information
the enzyme contains the conserved His box of plant omega-3 FA desaturase. One amino acid residue, Asn or Ser, is different at 61 position of PfrFAD3-1 and PfrFAD3-2, respectively. Both PfrFAD3-1 and -2 have the function of omega-3 FA desaturases
additional information
the enzyme contains the conserved His box of plant omega-3 FA desaturase. One amino acid residue, Asn or Ser, is different at 61 position of PfrFAD3-1 and PfrFAD3-2, respectively. Both PfrFAD3-1 and -2 have the function of omega-3 FA desaturases
additional information
-
fatty acid profile of wild-type and fad3 mutant roots in response to several plant hormones, overview
-
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expressed in Saccharomyces cerevisiae
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expressed in Saccharomyces cerevisiae strain InvSc1
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expressed in Saccharomyces cerevisiae strain K601
expressed in Saccharomyces verevisiae strain InvSc1
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expression in Saccharomyces cerevisiae
gene fad3, DNA and amino acid sequence determination and analysis
gene fad3, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant plamid pCAM::Nap-BjFad3-NOS is introduced into Agrobacterium tumefaciens strain LBA4404/VirGN54D by freeze-thaw method and then used for transformation of Escherichia coli strain DH10B and of Nicotiana tabacum explant, recombinant ectopic expression analysis in leaves of transgenic plants, recombinant expression of the enzyme in transgenic Oryza sativa plants, semi-quantitative RT-PCR expression analysis
gene fad3, overexpression of sense and antisense FAD3 sequences under control of the CaMV 35S promoter, quantitative reverse transcription-PCR enzyme expression analysis, LeFAD3 overexpression enhances the tolerance of early seedlings to salinity stress. Reduced oxidative damage in LeFAD3 overexpression tomato plants under salt stress, with altered ratio of 18:3/18:2 compared to wild-type. Change in leaf cell ultrastructure under salt stress in wild-type and mutant leaves
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gene fad3, recombinant expression of a fad3-LUC fusion construct in Arabidopsis thaliana plants through trabsfection with Agrobacterium tumefaciens strain C58 pGV3850
gene fad3-1, DNA and amino acid sequence determination and analysis, single gene, transcriptome analysis, sequence comparisons of fad genes, phylogenetic analysis, quantitative real-time and RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene fad3-2, DNA and amino acid sequence determination and analysis, single gene, transcriptome analysis, sequence comparisons of fad genes, phylogenetic analysis, quantitative real-time and RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1
gene omega-3 FAD, genetic structure with endogenous gene and native promoter, recombinant expression of the disrupted omega-3 FAD gene in Chlorella vulgaris via Agrobacterium-mediated transformation, real-time PCR and RT-PCR enzyme expression analysis
gene sdd17, recombinant exression of codon-otimized gene in human VCaP prostate cancer cells by lentiviral tranfection using the the PLJM1 lentivirus vector, which allows the endogenous production of OMEGA-3 polyunsaturated fatty acids (PUFAs), quantitative RT-PCR expression analysis
isolation of a gene and functionally complementation of a mutant of Arabidopsis deficient in omega-3 unsaturated fatty acids
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Sakuradani, E.; Abe, T.; Iguchi, K.; Shimizu, S.
A novel fungal omega3-desaturase with wide substrate specificity from arachidonic acid-producing Mortierella alpina 1S-4
Appl. Microbiol. Biotechnol.
66
648-654
2005
Mortierella alpina (Q59J82), Mortierella alpina, Mortierella alpina 1S-4 (Q59J82), Mortierella alpina 1S-4
brenda
Browse, J.; McConn, M.; James, D. Jr.; Miquel, M.
Mutants of Arabidopsis deficient in the synthesis of alpha-linolenate. Biochemical and genetic characterization of the endoplasmic reticulum linoleoyl desaturase
J. Biol. Chem.
268
16345-16351
1993
Arabidopsis thaliana
brenda
Arondel, V.; Lemieux, B.; Hwang, I.; Gibson, S.; Goodman, H.M.; Somerville, C.R.
Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis
Science
258
1353-1355
1992
Brassica napus (P48624)
brenda
Singer, S.; Weselake, R.; Rahman, H.
Development and characterization of low alpha-linolenic acid Brassica oleracea lines bearing a novel mutation in a class a FATTY ACID DESATURASE 3 gene
BMC Genet.
15
94
2014
Brassica oleracea
brenda
Bilyeu, K.; Gillman, J.; LeRoy, A.
Novel FAD3 mutant allele combinations produce soybeans containing 1% linolenic acid in the seed oil
Crop Sci.
51
259-264
2011
Glycine max
brenda
Hamada, T.; Kodama, H.; Nishimura, M.; Iba, K.
Cloning of a cDNA encoding tobacco omega-3 fatty acid desaturase
Gene
147
293-294
1994
Nicotiana tabacum (P48626), Nicotiana tabacum
brenda
Savile, C.; Reed, D.; Meesapyodsuk, D.; Covello, P.; Buist, P.
Cryptoregiochemistry of a Brassica napus fatty acid desaturase (FAD3): A kinetic isotope effect study
J. Chem. Soc. Perkin Trans. I
2001
1116-1121
2001
Brassica napus
-
brenda
Khadake, R.; Khonde, V.; Mhaske, V.; Ranjekar, P.; Harsulkar, A.
Functional and bioinformatic characterisation of sequence variants of Fad3 gene from flax
J. Sci. Food Agric.
91
2689-2696
2011
Linum usitatissimum
brenda
Radovanovic, N.; Thambugala, D.; Duguid, S.; Loewen, E.; Cloutier, S.
Functional characterization of flax fatty acid desaturase FAD2 and FAD3 isoforms expressed in yeast reveals a broad diversity in activity
Mol. Biotechnol.
56
609-620
2014
Linum usitatissimum
brenda
Matsuda, O.; Watanabe, C.; Iba, K.
Hormonal regulation of tissue-specific ectopic expression of an Arabidopsis endoplasmic reticulum-type omega-3 fatty acid desaturase (FAD3) gene
Planta
213
833-840
2001
Arabidopsis thaliana (P48623), Arabidopsis thaliana, Arabidopsis thaliana Col-0 (P48623)
brenda
Zhang, H.; Bi, Y.; Liu, Z.; Shan, L.
Heterologous expression of two Glycine max omega-3 fatty acid desaturases in Saccharomyces cerevisiae
Russ. J. Plant Physiol.
56
569-574
2009
Glycine max (Q84X83), Glycine max (Q84X84), Glycine max (Q9FXK9)
-
brenda
Lau, C.; Loh, S.; Aziz, A.; Cha, T.
Effects of disrupted omega-3 desaturase gene construct on fatty acid composition and expression of four fatty acid biosynthetic genes in transgenic Chlorella vulgaris
Algal Res.
26
143-152
2017
Chlorella vulgaris (A0A1L4BMI8), Chlorella vulgaris UMT-M1 (A0A1L4BMI8)
-
brenda
Pan, J.; Zhou, S.; Xiang, R.; Zhao, Z.; Liu, S.; Ding, N.; Gong, S.; Lin, Y.; Li, X.; Bai, X.; Li, F.; Zhao, A.Z.
An OMEGA-3 fatty acid desaturase-expressing gene attenuates prostate cancer proliferation by cell cycle regulation
Oncol. Lett.
13
3717-3721
2017
Saprolegnia diclina (Q6UB73)
brenda
Wang, H.; Yu, C.; Tang, X.; Zhu, Z.; Ma, N.; Meng, Q.
A tomato endoplasmic reticulum (ER)-type omega-3 fatty acid desaturase (LeFAD3) functions in early seedling tolerance to salinity stress
Plant Cell Rep.
33
131-142
2014
Solanum lycopersicum
brenda
Lee, K.R.; Lee, Y.; Kim, E.H.; Lee, S.B.; Roh, K.H.; Kim, J.B.; Kang, H.C.; Kim, H.U.
Functional identification of oleate 12-desaturase and omega-3 fatty acid desaturase genes from Perilla frutescens var. frutescens
Plant Cell Rep.
35
2523-2537
2016
Perilla frutescens var. frutescens (Q8VWX1), Perilla frutescens var. frutescens (Q9ZPP7)
brenda
Bhattacharya, S.; Chattopadhyaya, B.; Koduru, L.; Das, N.; Maiti, M.
Heterologous expression of Brassica juncea microsomal omega-3 desaturase gene (BjFad3) improves the nutritionally desirable omega-6 omega-3 fatty acid ratio in rice bran oil
Plant Cell Tissue Organ Cult.
119
117-129
2014
Brassica juncea (E0A920)
-
brenda