Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
IUBMB CommentsThe enzyme, characterized from the green alga Chlamydomonas reinhardtii, is a front-end desaturase that introduces a cis double bond in omega9 unsaturated C18 or C20 fatty acids incorporated into lipids, at a position 4 carbon atoms from the existing omega9 bond, towards the carboxy end of the fatty acid (at the omega13 position). When acting on 20:2Delta(11,14) and 20:3Delta(11,14,17) substrates it introduces the new double bond between carbons 7 and 8. The enzyme contains a cytochrome b5 domain that acts as the direct electron donor for the active site of the desaturase.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
acyl-[glycerolipid],ferrocytochrome b5:oxygen oxidoreductase [omega(9-4),omega(9-5) cis-dehydrogenating]
The enzyme, characterized from the green alga Chlamydomonas reinhardtii, is a front-end desaturase that introduces a cis double bond in omega9 unsaturated C18 or C20 fatty acids incorporated into lipids, at a position 4 carbon atoms from the existing omega9 bond, towards the carboxy end of the fatty acid (at the omega13 position). When acting on 20:2Delta(11,14) and 20:3Delta(11,14,17) substrates it introduces the new double bond between carbons 7 and 8. The enzyme contains a cytochrome b5 domain that acts as the direct electron donor for the active site of the desaturase.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
oleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
low activity
-
-
?
additional information
?
-
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
DELTA5-desaturase activity
-
-
?
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
DELTA5-desaturase activity
-
-
?
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
low activity
-
-
?
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
low activity
-
-
?
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
low activity
-
-
?
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
low activity
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
preferred substrate, DELTA5-desaturase activity
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
preferred substrate, DELTA5-desaturase activity
-
-
?
additional information
?
-
no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively
-
-
?
additional information
?
-
-
no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively
-
-
?
additional information
?
-
no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
oleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
low activity
-
-
?
additional information
?
-
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
DELTA5-desaturase activity
-
-
?
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
coniferonoyl-[glycerolipid] + ferricytochrome b5 + H2O
DELTA5-desaturase activity
-
-
?
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
low activity
-
-
?
dihomolinoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
low activity
-
-
?
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
low activity
-
-
?
eicosa-11,14,17-trienoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
?
low activity
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
preferred substrate, DELTA5-desaturase activity
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
pinolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
preferred substrate, DELTA5-desaturase activity
-
-
?
additional information
?
-
no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively
-
-
?
additional information
?
-
-
no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively
-
-
?
additional information
?
-
no activity with 18:1DELTA11, 16:0, 16:1DELTA9, 18:0, 18:2DELTA10E,12, 18:2DELTA9,11E, 20:1DELTA11, 20:3DELTA8,11,14, and 20:4DELTA8,11,14,17. The enzyme also shows DELTA7-desaturase activity on 20:2DELTA11,14 and 20:3DELTA11,14,17 to produce 20:3DELTA7,11,14 and 20:4DELTA7,11,14,17, respectively
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Kajikawa, M.; Yamato, K.; Kohzu, Y.; Shoji, S.; Matsui, K.; Tanaka, Y.; Sakai, Y.; Fukuzawa, H.
A front-end desaturase from Chlamydomonas reinhardtii produces pinolenic and coniferonic acids by omega13 desaturation in methylotrophic yeast and tobacco
Plant Cell Physiol.
47
64-73
2006
Chlamydomonas reinhardtii (Q2HWK7), Chlamydomonas reinhardtii, Chlamydomonas reinhardtii 137C (Q2HWK7)
brenda