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IUBMB CommentsThe enzyme, found in pathogenic and nonpathogenic mycobacteria species, actinomycetes, and some proteobacteria, hydroxylates the omega-carbon of a number of methyl-branched lipids, including (2E,6E)-farnesol, phytanate, geranylgeraniol, 15-methylpalmitate and (2E,6E)-farnesyl diphosphate. It is a P-450 heme-thiolate enzyme.
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15-methylpalmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
cholest-4-en-3-one + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
? + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
-
?
farnesol + O2 + reduced adrenodoxin [iron-sulfur] cluster + H+
? + H2O + oxidized adrenodoxin [iron-sulfur] cluster
farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
? + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
-
?
farnesyl diphosphate + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
geraniol + O2 + reduced adrenodoxin [iron-sulfur] cluster + H+
? + H2O + oxidized adrenodoxin [iron-sulfur] cluster
geranylgeraniol + O2 + reduced adrenodoxin [iron-sulfur] cluster + H+
? + H2O + oxidized adrenodoxin [iron-sulfur] cluster
geranylgeraniol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
-
-
-
?
geranylgeraniol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
? + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
-
?
lauric acid + O2 + reduced adrenodoxin [iron-sulfur] cluster + H+
? + H2O + oxidized adrenodoxin [iron-sulfur] cluster
palmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
the enzyme oxidizes the omega-1,omega-2, and omega-position of palmitic acid
-
-
?
phytanic acid + O2 + reduced adrenodoxin [iron-sulfur] cluster + H+
? + H2O + oxidized adrenodoxin [iron-sulfur] cluster
-
-
-
?
phytanic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
-
-
-
?
additional information
?
-
15-methylpalmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
-
-
-
?
15-methylpalmitic acid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
-
-
-
?
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
the enzyme uses spinach NAD(P)H-dependent ferredoxin reductase and the [2Fe-2S] iron-sulfur protein ferredoxin as an electron transport system
-
-
?
a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
farnesol + O2 + reduced adrenodoxin [iron-sulfur] cluster + H+
? + H2O + oxidized adrenodoxin [iron-sulfur] cluster
-
-
-
?
farnesol + O2 + reduced adrenodoxin [iron-sulfur] cluster + H+
? + H2O + oxidized adrenodoxin [iron-sulfur] cluster
-
-
-
?
farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
best substrate
-
-
?
farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
best substrate
-
-
?
farnesyl diphosphate + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
-
-
-
?
farnesyl diphosphate + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
?
-
-
-
?
geraniol + O2 + reduced adrenodoxin [iron-sulfur] cluster + H+
? + H2O + oxidized adrenodoxin [iron-sulfur] cluster
-
-
-
?
geraniol + O2 + reduced adrenodoxin [iron-sulfur] cluster + H+
? + H2O + oxidized adrenodoxin [iron-sulfur] cluster
-
-
-
?
geranylgeraniol + O2 + reduced adrenodoxin [iron-sulfur] cluster + H+
? + H2O + oxidized adrenodoxin [iron-sulfur] cluster
-
-
-
?
geranylgeraniol + O2 + reduced adrenodoxin [iron-sulfur] cluster + H+
? + H2O + oxidized adrenodoxin [iron-sulfur] cluster
-
-
-
?
lauric acid + O2 + reduced adrenodoxin [iron-sulfur] cluster + H+
? + H2O + oxidized adrenodoxin [iron-sulfur] cluster
-
-
-
?
lauric acid + O2 + reduced adrenodoxin [iron-sulfur] cluster + H+
? + H2O + oxidized adrenodoxin [iron-sulfur] cluster
-
-
-
?
additional information
?
-
the enzyme shows no activity with geraniol, lauric acid, phytane, pristane, phylloquinone (vitamin K1), menaquinone (vitamin K2), or bifonazole
-
-
?
additional information
?
-
-
the enzyme shows no activity with geraniol, lauric acid, phytane, pristane, phylloquinone (vitamin K1), menaquinone (vitamin K2), or bifonazole
-
-
?
additional information
?
-
-
the enzyme shows very low activity towards palmitic acid and 15-methyl-palmitic acid
-
-
?
additional information
?
-
the fusion complex of cytochrome b5 and NADH-cytochrome b5 reductase reduces Cyp124 (reduction efficiency -12.9%). The electron transfer pair adrenodoxin/NADPH-adrenodoxin reductase has a similar efficiency for Cyp124 reduction (reduction efficiency -9.4%) as the fusion complex. Rat NADPH-cytochrome P450 reductase is less effective for Cyp124 reduction. The enzyme can oxidize provitamin D3 (7-dehydrocholesterol) and vitamin D3. The final product is different from 1alpha- and 25-hydroxyvitamin D3
-
-
?
additional information
?
-
-
the fusion complex of cytochrome b5 and NADH-cytochrome b5 reductase reduces Cyp124 (reduction efficiency -12.9%). The electron transfer pair adrenodoxin/NADPH-adrenodoxin reductase has a similar efficiency for Cyp124 reduction (reduction efficiency -9.4%) as the fusion complex. Rat NADPH-cytochrome P450 reductase is less effective for Cyp124 reduction. The enzyme can oxidize provitamin D3 (7-dehydrocholesterol) and vitamin D3. The final product is different from 1alpha- and 25-hydroxyvitamin D3
-
-
?
additional information
?
-
the enzyme shows no activity with geraniol, lauric acid, phytane, pristane, phylloquinone (vitamin K1), menaquinone (vitamin K2), or bifonazole
-
-
?
additional information
?
-
the fusion complex of cytochrome b5 and NADH-cytochrome b5 reductase reduces Cyp124 (reduction efficiency -12.9%). The electron transfer pair adrenodoxin/NADPH-adrenodoxin reductase has a similar efficiency for Cyp124 reduction (reduction efficiency -9.4%) as the fusion complex. Rat NADPH-cytochrome P450 reductase is less effective for Cyp124 reduction. The enzyme can oxidize provitamin D3 (7-dehydrocholesterol) and vitamin D3. The final product is different from 1alpha- and 25-hydroxyvitamin D3
-
-
?
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Jensen, K.; Johnston, J.B.; de Montellano, P.R.; M?ller, B.L.
Photosystem I from plants as a bacterial cytochrome P450 surrogate electron donor: terminal hydroxylation of branched hydrocarbon chains
Biotechnol. Lett.
34
239-245
2012
Mycobacterium tuberculosis
brenda
Johnston, J.B.; Kells, P.M.; Podust, L.M.; Ortiz de Montellano, P.R.
Biochemical and structural characterization of CYP124: a methyl-branched lipid omega-hydroxylase from Mycobacterium tuberculosis
Proc. Natl. Acad. Sci. USA
106
20687-20692
2009
Mycobacterium tuberculosis (P9WPP3), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WPP3)
brenda
Vasilevskaya, A.V.; Yantsevich, A.V.; Sergeev, G.V.; Lemish, A.P.; Usanov, S.A.; Gilep, A.A.
Identification of Mycobacterium tuberculosis enzyme involved in vitamin D and 7-dehydrocholesterol metabolism
J. Steroid Biochem. Mol. Biol.
169
202-209
2017
Mycobacterium tuberculosis (P9WPP3), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WPP3)
brenda