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Information on EC 1.14.13.127 - 3-(3-hydroxyphenyl)propanoate hydroxylase

for references in articles please use BRENDA:EC1.14.13.127
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IUBMB Comments
A flavoprotein (FAD). This enzyme participates in a meta-cleavage pathway employed by the bacterium Escherichia coli for the degradation of various phenylpropanoid compounds.
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
mhpak-12, 3hpp 2-hydroxylase, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2E)-3-(3-hydroxyphenyl)prop-2-enoate + NADH + H+ + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H2O + NAD+
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(2)
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3-(3-hydroxyphenyl)propanoate + NADH + H+ + O2 = 3-(2,3-dihydroxyphenyl)propanoate + H2O + NAD+
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the His-Cys catalytic diad in free papain is fully protonated, -NH(+)/-SH. The experimental pKa of 8.62 for His159 imidazole in free papain is assigned to the -NH(+)/-SH and  -N/-SH equilibrium
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