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1-ethylthymine + 2-oxoglutarate + O2
? + succinate + CO2
-
-
-
?
1-ethyluracil + 2-oxoglutarate + O2
? + succinate + CO2
-
-
-
?
1-methylthymine + 2-oxoglutarate + O2
1-methyl-5-(hydroxymethyl)uracil + thymine + formaldehyde + succinate + CO2
1-methyluracil + 2-oxoglutarate + O2
? + succinate + CO2
-
-
-
?
5,6-dihydrothymine + 2-oxoglutarate + O2
5,6-dihydro-5-(hydroxymethyl)uracil + succinate + CO2
-
-
-
?
5,6-dihydroxythymine + 2-oxoglutarate + O2
5,6-dihydro-5-(hydroxymethyl)uracil + succinate + CO2
-
-
-
-
r
5-(2-hydroxyethyl)uracil + 2-oxoglutarate + O2
5-(1,2-dihydroxyethyl)uracil + succinate + CO2
5-(methylthio)uracil + 2-oxoglutarate + O2
5-(methylsulfonyl)uracil + 5-(methylsulfinyl)uracil + succinate + CO2
-
-
-
?
5-aminouracil + 2-oxoglutarate + O2
? + succinate + CO2
5-bromo-1-methyluracil + 2-oxoglutarate + O2
? + succinate + CO2
-
-
-
?
5-carboxyluracil + O2
?
-
-
-
?
5-ethynyluracil + 2-oxoglutarate + O2 + H2O
5-(carboxymethyl)uracil + succinate + CO2
5-fluorouracil + 2-oxoglutarate + O2
? + succinate + CO2
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
5-formyluracil + O2
?
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
5-hydroxyuracil + O2
?
-
-
-
?
5-methylcytosine + 2-oxoglutarate + O2
?
-
-
-
-
?
5-vinyluracil + 2-oxoglutarate + O2
5-(1,2-dihydroxyethyl)uracil + succinate + CO2
6-azathymine + 2-oxoglutarate + O2
? + succinate + CO2
-
-
-
?
N1-methylthymine + 2-oxoglutarate + O2
N1-methyl-5-hydroxymethyluracil + succinate + CO2
-
-
-
?
N1-methyluracil + 2-oxoglutarate + O2
? + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + 2 O2
uracil-5-carboxylate + succinate + CO2 + H2O
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
uracil + 2-oxoglutarate + O2
? + succinate + CO2
additional information
?
-
1-methylthymine + 2-oxoglutarate + O2
1-methyl-5-(hydroxymethyl)uracil + thymine + formaldehyde + succinate + CO2
-
-
-
-
?
1-methylthymine + 2-oxoglutarate + O2
1-methyl-5-(hydroxymethyl)uracil + thymine + formaldehyde + succinate + CO2
-
-
-
-
?
1-methylthymine + 2-oxoglutarate + O2
1-methyl-5-(hydroxymethyl)uracil + thymine + formaldehyde + succinate + CO2
-
-
-
?
5-(2-hydroxyethyl)uracil + 2-oxoglutarate + O2
5-(1,2-dihydroxyethyl)uracil + succinate + CO2
-
-
-
?
5-(2-hydroxyethyl)uracil + 2-oxoglutarate + O2
5-(1,2-dihydroxyethyl)uracil + succinate + CO2
-
-
-
?
5-aminouracil + 2-oxoglutarate + O2
? + succinate + CO2
-
-
-
?
5-aminouracil + 2-oxoglutarate + O2
? + succinate + CO2
-
-
-
?
5-aminouracil + 2-oxoglutarate + O2
? + succinate + CO2
-
-
-
?
5-aminouracil + 2-oxoglutarate + O2
? + succinate + CO2
-
-
-
?
5-ethynyluracil + 2-oxoglutarate + O2 + H2O
5-(carboxymethyl)uracil + succinate + CO2
-
-
-
?
5-ethynyluracil + 2-oxoglutarate + O2 + H2O
5-(carboxymethyl)uracil + succinate + CO2
-
-
-
?
5-ethynyluracil + 2-oxoglutarate + O2 + H2O
5-(carboxymethyl)uracil + succinate + CO2
-
-
-
?
5-fluorouracil + 2-oxoglutarate + O2
? + succinate + CO2
-
-
-
?
5-fluorouracil + 2-oxoglutarate + O2
? + succinate + CO2
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
5-formyluracil reacts subsequently to 5-carboxyuracil
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
5-formyluracil reacts subsequently to 5-carboxyuracil
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
5-formyluracil reacts subsequently to 5-carboxyuracil
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
5-formyluracil reacts subsequently to 5-carboxyuracil
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
5-formyluracil reacts subsequently to 5-carboxyuracil
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
5-formyluracil reacts subsequently to 5-carboxyuracil
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
5-formyluracil reacts subsequently to 5-carboxyuracil
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
5-formyluracil reacts subsequently to 5-carboxyuracil
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
5-formyluracil reacts subsequently to 5-carboxyuracil
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
5-formyluracil reacts subsequently to 5-carboxyuracil
?
5-vinyluracil + 2-oxoglutarate + O2
5-(1,2-dihydroxyethyl)uracil + succinate + CO2
-
-
-
?
5-vinyluracil + 2-oxoglutarate + O2
5-(1,2-dihydroxyethyl)uracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + 2 O2
uracil-5-carboxylate + succinate + CO2 + H2O
-
three step reaction with 5-hydroxymethyluracil and 5-formyluracil as intermediate products
-
?
thymine + 2-oxoglutarate + 2 O2
uracil-5-carboxylate + succinate + CO2 + H2O
conversion of thymine to uracil
three step reaction with 5-hydroxymethyluracil and 5-formyluracil as intermediate products
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
the telomeric repeat sequence GGGTTA is able to stimulate J synthesis, while mutated telomeric sequences (GGGTTT, GGGATT, or GGGAAA) are unable to support J synthesis
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
5-hydroxymethyluracil reacts subsequently to 5-formyluracil
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
i.e. 5-methyluracil
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
5-hydroxymethyluracil reacts subsequently to 5-formyluracil
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
5-hydroxymethyluracil reacts subsequently to 5-formyluracil
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
5-hydroxymethyluracil reacts subsequently to 5-formyluracil
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
5-hydroxymethyluracil reacts subsequently to 5-formyluracil
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
5-hydroxymethyluracil reacts subsequently to 5-formyluracil
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
stereospecific removal of the pro-S hydrogen
5-hydroxymethyluracil reacts subsequently to 5-formyluracil
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
uracil + 2-oxoglutarate + O2
? + succinate + CO2
-
-
-
?
uracil + 2-oxoglutarate + O2
? + succinate + CO2
-
-
-
?
additional information
?
-
-
enzyme is responsible for the local amplification of the unusual DNA base J
-
-
?
additional information
?
-
the enzyme is a bifunctional helicase and thymine dioxygenase
-
-
?
additional information
?
-
-
the enzyme is a bifunctional helicase and thymine dioxygenase
-
-
?
additional information
?
-
no activity with N1-methyl-5-hydroxymethyluracil, 5-methylcytosine, N3-methyluracil, 6-methyluracil, uracil, 5-carboxyuracil, and thymidine, substrate specificity, overview. Analysis of enzyme reaction products by GC/MS
-
-
?
additional information
?
-
-
no activity with N1-methyl-5-hydroxymethyluracil, 5-methylcytosine, N3-methyluracil, 6-methyluracil, uracil, 5-carboxyuracil, and thymidine, substrate specificity, overview. Analysis of enzyme reaction products by GC/MS
-
-
?
additional information
?
-
-
enzyme is responsible for the local amplification of the unusual DNA base J
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5-carboxyluracil + O2
?
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
5-formyluracil + O2
?
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
thymine + 2-oxoglutarate + 2 O2
uracil-5-carboxylate + succinate + CO2 + H2O
conversion of thymine to uracil
three step reaction with 5-hydroxymethyluracil and 5-formyluracil as intermediate products
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
additional information
?
-
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
?
5-formyluracil + 2-oxoglutarate + O2
5-carboxyuracil + succinate + CO2
-
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
5-formyluracil reacts subsequently to 5-carboxyuracil
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
5-formyluracil reacts subsequently to 5-carboxyuracil
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
-
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
5-formyluracil reacts subsequently to 5-carboxyuracil
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
5-formyluracil reacts subsequently to 5-carboxyuracil
?
5-hydroxymethyluracil + 2-oxoglutarate + O2
5-formyluracil + succinate + CO2
-
-
5-formyluracil reacts subsequently to 5-carboxyuracil
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
5-hydroxymethyluracil reacts subsequently to 5-formyluracil
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
5-hydroxymethyluracil reacts subsequently to 5-formyluracil
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
5-hydroxymethyluracil reacts subsequently to 5-formyluracil
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
5-hydroxymethyluracil reacts subsequently to 5-formyluracil
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
5-hydroxymethyluracil reacts subsequently to 5-formyluracil
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
5-hydroxymethyluracil reacts subsequently to 5-formyluracil
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
stereospecific removal of the pro-S hydrogen
5-hydroxymethyluracil reacts subsequently to 5-formyluracil
?
thymine + 2-oxoglutarate + O2
5-hydroxymethyluracil + succinate + CO2
-
-
-
?
additional information
?
-
-
enzyme is responsible for the local amplification of the unusual DNA base J
-
-
?
additional information
?
-
-
enzyme is responsible for the local amplification of the unusual DNA base J
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D215S
-
mutant proteins fail to restore the J-level in the JBP1 null trypanosomes to wild-type levels
D416A
site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect
H213S
-
mutant proteins fail to restore the J-level in the JBP1 null trypanosomes to wild-type levels
H414A
site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect
H465A
site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect
R478A
site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect
V482A/L548Q
site-directed and random mutagenesis, the mutant can complement the JBP2 knockout mutant strain
D216N
site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme
E122A
site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme
F292A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
H214A
site-directed mutagenesis, the mutant shows highly reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme
H271A
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
L192A
site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme
L223A
site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme
N294A
site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme
N87A
site-directed mutagenesis, the mutant shows similar activity with thymine and altered substrate specificity compared to the wild-type enzyme
R190A
site-directed mutagenesis, the mutant shows highly reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme
R190K
site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme
R286A
site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme
R286K
site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme
S288A
site-directed mutagenesis, the mutant shows similar activity with thymine and altered substrate specificity compared to the wild-type enzyme
V273A
site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme
Y194F
site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme
Y217A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y217F
site-directed mutagenesis, the mutant shows similar activity compared to the wild-type enzyme
N187D
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
N250D
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
N293D
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme
R285C
site-directed mutagenesis, inactive active site mutant
D191A
-
mutation abrogates the ability of JBP1 to complement JBP1 null mutants
D393A
site-directed mutagenesis, mutation inside the catalytic domain, procyclic cells expressing mutant versions of JBP2 inside the catalytic domain
H189A
-
mutation abrogates the ability of JBP1 to complement JBP1 null mutants
H189A/D191A
-
mutation abrogates the ability of JBP1 to complement JBP1 null mutants
H239A
-
mutation abrogates the ability of JBP1 to complement JBP1 null mutants
H391A
site-directed mutagenesis, mutation inside the catalytic domain, procyclic cells expressing mutant versions of JBP2, expression of the TH mutant H319A fails to stimulate base J formation
H441A
site-directed mutagenesis, mutation inside the catalytic domain, procyclic cells expressing mutant versions of JBP2 inside the catalytic domain
R255A
-
mutation abrogates the ability of JBP1 to complement JBP1 null mutants
R445A
site-directed mutagenesis, mutation inside the catalytic domain, procyclic cells expressing mutant versions of JBP2 inside the catalytic domain
V259A
-
mutation abrogates the ability of JBP1 to complement JBP1 null mutants
V459A
site-directed mutagenesis, control substitution of a residue outside the catalytic domain
additional information
inactivation of both of the LtJBP2 alleles by conventional knock-out strategy, the major phenotypes of the LtJBP2-/- cells, showing reduced levels of J and serious growth defects, can be completely reversed by the presence of an ectopic copy of LtJBP2
additional information
-
inactivation of both of the LtJBP2 alleles by conventional knock-out strategy, the major phenotypes of the LtJBP2-/- cells, showing reduced levels of J and serious growth defects, can be completely reversed by the presence of an ectopic copy of LtJBP2
additional information
construction of a C-terminally truncated enzyme mutant NcT7H, comprising residues 1-299
additional information
-
construction of a C-terminally truncated enzyme mutant NcT7H, comprising residues 1-299
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Lai, M.T.; Wu, W.; Stubbe, J.
Characterization of a novel, stable norcaradiene adduct resulting from the inactivation of thymine hydroxylase by 5-ethynyluracil
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Rhodotorula glutinis
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Mechanism-based inactivation of thymine hydroxylase, an alpha-ketoglutarate-dependent dioxygenase, by 5-ethynyluracil
Biochemistry
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1993
Rhodotorula glutinis
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A non-heme iron protein with heme tendencies: An investigation of the substrate specificity of thymine hydroxylase
Biochemistry
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1993
Rhodotorula glutinis
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Thornburg, L.D.; Stubbe, J.
Mechanism-based inhibition of thymine hydroxylase
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1989
Rhodotorula glutinis
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Warn-Cramer, B.J.; Macrander, L.A.; Abbott, M.T.
Markedly different ascorbate dependencies of the sequential alpha-ketoglutarate dioxygenase reactions catalyzed by an essentially homogeneous thymine 7-hydroxylase from Rhodotorula glutinis
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1983
Rhodotorula glutinis
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Holme, E.; Lindstedt, S.
Studies on the partial reaction of thymine 7-hydroxylase in the presence of 5-fluorouracil
Biochim. Biophys. Acta
704
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1982
Neurospora crassa, Neurospora crassa STA 4
brenda
Hsu, C.A.; Saewert, M.D.; Polsinelli Jr., L.F.; Abbott, M.T.
Uracils uncoupling of the decarboxylation of alpha-ketoglutarate in the thymine 7-hydroxylase reaction of Neurospora crassa
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256
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1981
Neurospora crassa, Neurospora crassa uc-1
brenda
Wondrack, L.M.; Warn, B.J.; Saewert, M.D.; Abbott, M.T.
Substitution of nucleoside triphosphates for ascorbate in the thymine 7-hydroxylase reaction of Rhodotorula glutinis
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254
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1979
Rhodotorula glutinis
brenda
Wondrack, L.M.; Hsu, C.A.; Abbott, M.T.
Thymine 7-hydroxylase and pyrimidine desoxyribonucleoside 2'-hydroxylase activities in Rhodotorula glutinis
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1978
Rhodotorula glutinis
brenda
Bankel, L.; Lindstedt, G.; Lindstedt, S.
Thymine 7-hydroxylase from Neurospora crassa. Substrate specificity studies
Biochim. Biophys. Acta
481
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1977
Neurospora crassa, Neurospora crassa STA 4
brenda
Holme, E.
A kinetic study of thymine 7-hydroxylase from Neurospora crassa
Biochemistry
14
4999-5003
1975
Neurospora crassa, Neurospora crassa STA 4
brenda
Liu, C.K.; Hsu, C.A.; Abbott, M.T.
Catalysis of three sequential dioxygenase reactions by thymine 7-hydroxylase
Arch. Biochem. Biophys.
159
180-187
1973
Neurospora crassa, Neurospora crassa 1A
brenda
Bankel, L.; Holme, E.; Lindstedt, G.; Lindstedt, S.
Oxygenases involved in thymine and thymidine metabolism in Neurospora crassa
FEBS Lett.
21
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1972
Neurospora crassa, Neurospora crassa STA 4
brenda
McCroskey, R.P.; Griswold, W.R.; Sokoloff, R.L.; Sevier, E.D.; Lin, S.; Liu, C.K.; Shaffer, P.M.; Palmatier, R.D.; Parker, T.S.; Abbott, M.T.
Studies pertaining to the purification and properties of thymine 7-hydroxylase
Biochim. Biophys. Acta
277
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1971
Neurospora crassa, Neurospora crassa 1A
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brenda
Abbott, M.T.; Kadner, R.J.; Fink, R.M.
Conversion of thymine to 5-hydroxymethyluracil in a cell-free system
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1964
Neurospora crassa, Neurospora crassa 1A
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Smiley, J.A.; Kundracik, M.; Landfried, D.A.; Barnes, V.R., Sr.; Axhemi, A.A.
Genes of the thymidine salvage pathway: Thymine-7-hydroxylase from a Rhodotorula glutinis cDNA library and iso-orotate decarboxylase from Neurospora crassa
Biochim. Biophys. Acta
1723
256-264
2005
Rhodotorula glutinis (Q66Q78), Rhodotorula glutinis
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Yu, Z.; Genest, P.; ter Riet, B.; Sweeney, K.; DiPaolo, C.; Kieft, R.; Christodoulou, E.; Perrakis, A.; Simmons, J.M.; Hausinger, R.P.; van Luenen, H.G.; Rigden, D.J.; Sabatini, R.; Borst, P.
The protein that binds to DNA base J in trypanosomatids has features of a thymidine hydroxylase
Nucleic Acids Res.
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2007
Leishmania tarentolae, Trypanosoma brucei
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Neidigh, J.W.; Darwanto, A.; Williams, A.A.; Wall, N.R.; Sowers, L.C.
Cloning and characterization of Rhodotorula glutinis thymine hydroxylase
Chem. Res. Toxicol.
22
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2009
Rhodotorula glutinis (Q66Q78), Rhodotorula glutinis
brenda
Vainio, S.; Genest, P.A.; ter Riet, B.; van Luenen, H.; Borst, P.
Evidence that J-binding protein 2 is a thymidine hydroxylase catalyzing the first step in the biosynthesis of DNA base J
Mol. Biochem. Parasitol.
164
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2009
Leishmania tarentolae (B6EU02), Leishmania tarentolae
brenda
Cliffe, L.J.; Kieft, R.; Southern, T.; Birkeland, S.R.; Marshall, M.; Sweeney, K.; Sabatini, R.
JBP1 and JBP2 are two distinct thymidine hydroxylases involved in J biosynthesis in genomic DNA of African trypanosomes
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2009
Trypanosoma brucei, Trypanosoma brucei (Q57X81)
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Bullard, W.; Cliffe, L.; Wang, P.; Wang, Y.; Sabatini, R.
Base J glucosyltransferase does not regulate the sequence specificity of J synthesis in trypanosomatid telomeric DNA
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2015
Leishmania major (Q4QFY1), Leishmania major (Q4QHM7)
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Li, W.; Zhang, T.; Ding, J.
Molecular basis for the substrate specificity and catalytic mechanism of thymine-7-hydroxylase in fungi
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2015
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