Information on EC 1.1.1.46 - L-arabinose 1-dehydrogenase

Word Map on EC 1.1.1.46
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:


The expected taxonomic range for this enzyme is: Archaea, Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.46
-
RECOMMENDED NAME
GeneOntology No.
L-arabinose 1-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-arabinose + NAD+ = L-arabinono-1,4-lactone + NADH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Ascorbate and aldarate metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
L-arabinose:NAD+ 1-oxidoreductase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9028-52-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH + H+
show the reaction diagram
L-arabinose + NADP+
L-arabinono-1,4-lactone + NADPH + H+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-arabinose + NAD+
L-arabinono-1,4-lactone + NADH + H+
show the reaction diagram
L-arabinose + NADP+
L-arabinono-1,4-lactone + NADPH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
the enzyme shows slight preference for NADP+ over NAD+
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
dependent on with with maximal activity at 1.5 M
Na+
dependent on with with maximal activity at 1.0 M
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
NAD+
at pH 8.5 and 45°C
2.1
NADP+
at pH 8.5 and 45°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
12
-
activity increases with pH up to pH 12. At pH 13 the reaction is completely inhibited, cell free preparation
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
50% activity at pH 6.0 and 10.0
12
-
activity increases with increasing pH up to pH 12. At pH 13 the reaction is completely inhibited, cell free preparation
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
130000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for at least 2 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Sepharose CL 4B column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta (DE3)-pLysS cells
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
refolding buffer contains 3 M KCl, L-arabinose, NADP+ and glutathione
Show AA Sequence (122 entries)
Please use the Sequence Search for a specific query.