Information on EC 1.1.1.327 - 5-exo-hydroxycamphor dehydrogenase

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The expected taxonomic range for this enzyme is: Pseudomonas putida

EC NUMBER
COMMENTARY hide
1.1.1.327
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RECOMMENDED NAME
GeneOntology No.
5-exo-hydroxycamphor dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-exo-hydroxycamphor + NAD+ = bornane-2,5-dione + NADH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
(+)-camphor degradation
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SYSTEMATIC NAME
IUBMB Comments
5-exo-hydroxycamphor:NAD+ oxidoreductase
Contains Zn2+. Isolated from Pseudomonas putida, and involved in degradation of (+)-camphor.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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Pseudomonas putida PpGl, which carries the CAM plasmid encoding enzymes involved in the degradation pathway of D-camphor, can utilize D-camphor as a sole carbon source. The enzymes responsible for early steps in D-camphor degradation are 5-exo-hydroxycamphor dehydrogenase (camD gene), cytochrome P-450cam (camC), NADH-putidaredoxin reductase (camA), and putidaredoxin (camB)
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-exo-hydroxycamphor + NAD+
bornane-2,5-dione + NADH + H+
show the reaction diagram
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r
bornane-2,5-dione + NADH + H+
5-exo-hydroxycamphor + NAD+
show the reaction diagram
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r
additional information
?
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no substrate: camphor
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
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two firmly bound zinc atoms per subunit. All three ligands, C40, H62 and C158 to the catalytic zinc atom in the active-site, and all four ligands, C98, C101, C104 and Cll2 to the second zinc atom, are conserved
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0279
5-exo-hydroxycamphor
pH 8.0, 45°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
no residual activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38400
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x * 38400, calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene camD encoding 5-exo-hydroxycamphor dehydrogenase is plasmid born, cotransferable by conjugation, mitomycin curable, and shows a higher segregation rate from cells that are multiplasmid rather than carrying a single plasmid
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the genes of the CAM plasmid encoding enzymes involved in the degradation pathway of D-camphor form an operon, camDCAB, and are under negative control by the gene camR located immediately upstream from the camD gene
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
synthetic route for the synthesis of bifunctional camphor derivatives. The combination of the enzymatic P450cam system with 5-exo-hydroxycamphor dehydrogenase (FdeH) allows an efficient synthesis of 2,5-diketobornane, which can be used for further derivatisation