The enzyme acts in the opposite direction. In Ocimum basilicum (sweet basil), Clarkia breweri and Petunia hybrida only isoeugenol is formed [1,2]. However in Pimpinella anisum (anise) only anol is formed in vivo, although the cloned enzyme does produce isoeugenol .
The enzyme acts in the opposite direction. In Ocimum basilicum (sweet basil), Clarkia breweri and Petunia hybrida only isoeugenol is formed [1,2]. However in Pimpinella anisum (anise) only anol is formed in vivo, although the cloned enzyme does produce isoeugenol [3].
PhIGS1 uses a quinone methide intermediate-based mechanism to generate an intermediate to which the reductive transfer of the reducing hydride can then be easily accomplished
purified recombinant DcE(I)GS1 catalyzes the formation of eugenol (70% of total products, EC 1.1.1.318), as well as isoeugenol (30% of total products,, EC 1.1.1.319) from coniferyl acetate
the position of the substrate in the active site of PhIGS1 is such that the hydride is transferred to C9 instead of C7, as occurs in Ocimum basilicum eugenol synthase, EC 1.1.1.318
quantitative RT-PCR enzyme expression analysis in different tissues. Distribution of aromatic volatiles in different parts of the carrot plants, overview
the phenylpropene-forming isoeugenol, IGS, synthase belongs to a structural family of NADPH-dependent reductases that also includes eugenol synthase, EGS, pinoresinollariciresinol reductase, isoflavone reductase, and phenylcoumaran benzylic ether reductase, evolution and function of EGS1 and IGS1, overview
the IGS gene of Petunia axillaris subsp. parodii contains a frame-shift mutation that renders it inactive. In the absence of IGS activity the coniferyl acetate substrate is converted to dihydroconiferyl acetate, instead that eugenol synthesis is increased, overview
plants synthesize the volatile phenylpropene compounds eugenol and isoeugenol to serve in defense against herbivores and pathogens and to attract pollinators. Clarkia breweri flowers emit a mixture of eugenol and isoeugenol. Eugenol and isoeugenol differ in the position of the double bond in the propene side chain
plants synthesize the volatile phenylpropene compounds eugenol and isoeugenol to serve in defense against herbivores and pathogens and to attract pollinators. Petunia hybrida flowers emit mostly isoeugenol with small amounts of eugenol. Eugenol and isoeugenol differ in the position of the double bond in the propene side chain
t-anol/isoeugenol synthase is an NADPH-dependent enzyme that can biosynthesize t-anol and isoeugenol from coumaryl acetate and coniferyl acetate, respectively
eugenol/isoeugenol synthase (DcE(I)GS1) is an NADPH-dependent enzyme that converts coniferyl acetate to eugenol. This enzyme exhibits dual product specificity and yields propenylphenol isoeugenol alongside allylphenol eugenol
site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme
site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme
site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme
site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme
site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme
site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme
site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme
site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme
site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme
site-directed mutagenesis, the mutant plants show increased eugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme
recombinant Petunia hybrida IGS expression alone does not increase the concentration of anol or isoeugenol in agroinfiltrated strawberry fruits, concurrent silencing of CHS substantially enhances the yield of the two metabolites
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 9.2fold from petals by alternating steps of anion exchange and hydrophobic interaction chromatography, co-purification with eugenol synthase, EC 1.1.1.318. Recombinant His-tagged CbIGS from Escherichia coli by nickel affinity chromatography
DNA and amino acid sequence determination and analysis, sequence comparison, quantitative expression analysis, levels of PapIGS transcripts are extremely low
IGS1 expression in Fragaria x ananassa cv. Elsanta via transfection with Agrobacterium tumefaciens strain AGL0, downregulation of chalcone synthase via antisense construct in parallel
Koeduka, T.; Baiga, T.J.; Noel, J.P.; Pichersky, E.
Biosynthesis of t-anethole in anise: characterization of t-anol/isoeugenol synthase and an O-methyltransferase specific for a C7-C8 propenyl side chain
Vassao, D.; Kim, S.; Milhollan, J.; Eichinger, D.; Davin, L.; Lewis, N.
A pinoresinol-lariciresinol reductase homologue from the creosote bush (Larrea tridentata) catalyzes the efficient in vitro conversion of p-coumaryl/coniferyl alcohol esters into the allylphenols chavicol/eugenol, but not the propenylphenols p-anol/isoeug