transformed tomato plants containing siRNA from the M6PR of Orobanche aegyptiaca show normal growth and development, accumulation of M6PR siRNA in transgenic tomatoes correlates with decreased levels of M6PR mRNA. Amount of endogenous M6PR mRNA in the tubercles and underground shoots of Orobanche aegyptiaca grown on transgenic host plants is reduced by 60%-80%. Concomitant with M6PR mRNA suppression, there is a significant decrease in mannitol level and a significant increase in the percentage of dead Orobanche aegyptiaca tubercles on the transgenic host plants. Detection of mir390, which is involved with cytoplasmic dsRNA processing, indicates to the existence of gene-silencing mechanisms in Orobanche spp. Gene silencing mechanisms are probably involved with the production of decreased levels of M6PR mRNA in the parasites grown on the transformed tomato lines
Exocytosis of Progeny Infectious Varicella-Zoster Virus Particles via a Mannose-6-Phosphate Receptor Pathway without Xenophagy following Secondary Envelopment.
Insulin-like growth factor II receptor-mediated intracellular retention of cathepsin B is essential for transformation of endothelial cells by Kaposi's sarcoma-associated herpesvirus.
constitutive gene expression during different developmental stages, e.g. germination and procaulome growth, of the parasite attached to tomato roots, high activity during tubercle enlargement and crown root formation
recombinant expression under control of the CaMV35S promotor in transgenic Arabidopsis thaliana cv. LB4404 plants induces de novo mannitol and glucose-mannitol production and enhances salt tolerance in the transgenic plants, cytoplasmic localization of the recombinant enzyme, overview
expression of mannose 6-phosphate 6-reductase in Arabidopsis thaliana results in mannitol producing plants that grow normally in the absence of stress, but show enhanced tolerance to presence of 0.1 or 0.2 mM NaCl than wild-type
expression in transgenic Arabidopsis thaliana plants, the transformed plants show reduced salt injury, less inhibition of vegetative growth, and increased seed production, thus increased salt tolerance, relative to the wild-type plants. When treated with 200 mM NaCl, transformants produce no seeds, but do bolt, and exhibit less chlorosis/necrosis and greater survival and dry weights than the wild-type. Without salt there are no M6PR effects on growth or phenotype, but expression levels of 2272 genes are altered, e.g upregulation of ABA receptor genes, PYL4, PYL5, and PYL6, and downregulation of protein phosphatase 2C genes, ABI1 and ABI2, overview
transgenic tomato plants bearing a gene construct containing a specific 277-bp fragment from M6PR-mRNA, in an inverted-repeat configuration. Eight independent lines containing the M6PR-silencing inverted-repeat construct pBin-IR-M6PR construct are regenerated
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the CaM6FR gene shows higher expression with drought stress in the Coffea arabica IAPAR59 cultivar and in the Coffea canephora clone 14 (considered as drought tolerant), than in the Coffea arabica RUBI MG 1192 cultivar
transformation of mannose-6-phosphate reductase gene into Arabidopsis and tobacco using Agrobacterium tumefaciens-mediated transformation. Mannose-6-phosphate reductase can act as a selectable marker gene in either a positive or a negative selection mode depending on the plant species. On medium containing 2 g/l mannose, transgenic seeds germinate, whereas wild type seeds did not. Mannose at 30 g/l blanches leaf explants from all 29 transgenic tobacco events with mannose-6-phosphate reductase. In contrast, 30 g/l mannose does not inhibit shoot regeneration from leaf explants of wild-type or transgenic plants with either an antisense mannose-6-phosphate reductase or a plasmid control. Mannose at 30 g/l inhibits seed germination of transgenic tobacco seeds with mannose-6-phosphate reductase but not that of wild-type or transgenic tobacco with either the antisense mannose-6-phosphate reductase or the plasmid control
Mannose-6-phosphate reductase, a key enzyme in photoassimilate partitioning, is abundant and located in the cytosol of photosynthetically active cells of celery (Apium graveolens L.) source leaves
Isolation of mannose 6-phosphate reductase cDNA, changes in enzyme activity and mannitol content in broomrape (Orobanche ramosa) parasitic on tomato roots
Expression of a celery mannose 6-phosphate reductase in Arabidopsis thaliana enhances salt tolerance and induces biosynthesis of both mannitol and a glucosyl-mannitol dimer
Plant Cell Environ.
26
275-283
2003
Apium graveolens, no activity in Arabidopsis thaliana
Gene silencing of mannose 6-phosphate reductase in the parasitic weed Orobanche aegyptiaca through the production of homologous dsRNA sequences in the host plant
Global gene expression analysis of transgenic, mannitol-producing, and salt-tolerant Arabidopsis thaliana indicates widespread changes in abiotic and biotic stress-related genes