Information on EC 1.1.1.223 - isopiperitenol dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.223
-
RECOMMENDED NAME
GeneOntology No.
isopiperitenol dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(-)-trans-isopiperitenol + NAD+ = (-)-isopiperitenone + NADH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Monoterpenoid biosynthesis
-
-
Biosynthesis of secondary metabolites
-
-
menthol biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
(-)-trans-isopiperitenol:NAD+ oxidoreductase
Acts on (-)-trans-isopiperitenol, (+)-trans-piperitenol and (+)-trans-pulegol. Involved in the biosynthesis of menthol and related monoterpenes in peppermint (Mentha piperita) leaves.
CAS REGISTRY NUMBER
COMMENTARY hide
96595-05-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
PT-type
UniProt
Manually annotated by BRENDA team
PT-type
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-isomenthol + NAD+
(+)-isomenthone + NADH
show the reaction diagram
-
activity less than 2% of that of (-)-trans-isopiperitenol
-
-
?
(+)-trans-isopiperitenol + NAD+
(+)-isopiperitenone + NADH
show the reaction diagram
-
95% activity of that of (-)-trans-isopiperitenol
-
?
(+)-trans-pulegol + NAD+
(R)-pulegone + NADH
show the reaction diagram
-
19% activity of that of (-)-trans-isopiperitenol
-
-
?
(+,-)-cis-isopiperitenol + NAD+
(+,-)-isopiperitenone
show the reaction diagram
-
-
-
-
?
(-)-cis-isopiperitenol + NAD+
(-)-isopiperitenone + NADH + H+
show the reaction diagram
(-)-trans-isopiperitenol + NAD+
(-)-isopiperitenone + NADH
show the reaction diagram
(-)-trans-isopiperitenol + NAD+
(-)-isopiperitenone + NADH + H+
show the reaction diagram
(-)-trans-isopiperitenol + NADP+
(-)-isopiperitenone + NADPH + H+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(-)-cis-isopiperitenol + NAD+
(-)-isopiperitenone + NADH + H+
show the reaction diagram
(-)-trans-isopiperitenol + NAD+
(-)-isopiperitenone + NADH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
additional information
-
FAD completely ineffective
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Hg2+
-
complete inhibition at 0.1 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iodacetamide
-
slight inhibition
N-ethylmaleimide
-
slight inhibition
p-hydroxymercuribenzoate
-
complete inhibition at 0.025 nM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.08
(+)-cis-isoperitenol
-
-
0.02
(+)-trans-isoperitenol
-
-
0.08
(-)-cis-isoperitenol
-
-
-
0.02
(-)-trans-isoperitenol
-
-
-
0.017
NAD+
-
-
2.5
NADP+
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5 - 10.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 11
-
half maximum activity at both pH
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
66000
-
gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stabilized with bovine serum albumin
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain TOP10F'
recombinant His-tagged enzyme from Escherichia coli strain TOP10F'; recombinant His-tagged enzyme from Escherichia coli strain TOP10F'
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
gene PK-ISPD1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain TOP10F'; gene PT-ISPD2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain TOP10F'
gene PT-ISPD2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain TOP10F'