Information on EC 1.1.1.15 - D-iditol 2-dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.1.1.15
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RECOMMENDED NAME
GeneOntology No.
D-iditol 2-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-iditol + NAD+ = D-sorbose + NADH + H+
show the reaction diagram
Also converts xylitol into L-xylulose and L-glucitol into L-fructose
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Pentose and glucuronate interconversions
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Fructose and mannose metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
D-iditol:NAD+ 2-oxidoreductase
Also converts xylitol into L-xylulose and L-glucitol into L-fructose.
CAS REGISTRY NUMBER
COMMENTARY hide
9028-22-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
i.e. Bradyrhizobium diazoefficiens
UniProt
Manually annotated by BRENDA team
i.e. Bradyrhizobium diazoefficiens
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-glucitol + NAD+
D-fructose + NADH + H+
show the reaction diagram
D-iditol + NAD+
D-sorbose + NADH
show the reaction diagram
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less stable enzyme, dulcitol-inducible, accounts for oxidation of certain fully hydroxylated polyols containing a D-threo-configuration adjacent to a primary alcohol
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?
D-iditol + NAD+
D-sorbose + NADH + H+
show the reaction diagram
D-sorbitol + NAD+
D-fructose + NADH + H+
show the reaction diagram
L-glucitol + NAD+
D-sorbose + NADH + H+
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-iditol + NAD+
D-sorbose + NADH
show the reaction diagram
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less stable enzyme, dulcitol-inducible, accounts for oxidation of certain fully hydroxylated polyols containing a D-threo-configuration adjacent to a primary alcohol
-
?
D-iditol + NAD+
D-sorbose + NADH + H+
show the reaction diagram
D-sorbitol + NAD+
D-fructose + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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an NAD(H)-dependent enzyme
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
zinc metalloenzyme
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26900
SDS-PAGE, subunit
77700
native PAGE, recombinant enzyme
83200
gel filtration, recombinant enzyme
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant His-tagged enzyme in complex with both NAD+ and glucitol, X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular replacement and modelling
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold (DE3) by heat treatment at 50°C and metal affinity chromatography to homogeneity
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene rdh, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-Gold (DE3)
gene SDh, DNA and amino acid sequence determination and analysis, isolation, characterization and evaluation of the Pichia pastoris sorbitol dehydrogenase promoter for expression of heterologous proteins, recombinant expression of several different enzymes using the SDH promoter, overview
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