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1.1.3.7: aryl-alcohol oxidase

This is an abbreviated version!
For detailed information about aryl-alcohol oxidase, go to the full flat file.

Word Map on EC 1.1.3.7

Reaction

an aromatic primary alcohol
+
O2
=
an aromatic aldehyde
 +
H2O2

Synonyms

AAO, AAO2, AAOx, alcohol: O2 oxidoreductase, AOX, arom. alcohol oxidase, aryl alcohol oxidase, arylalcohol oxidase, CpSAO, CtSAO, GaoB, GLRG_02805, GMC oxidoreductase-like protein, HMFO, More, MtGloA, MYCTH_2299749, oxidase, aryl alcohol, salicyl alcohol oxidase, um04044, VAO, veratryl alcohol oxidase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.7 aryl-alcohol oxidase

Cloned

Cloned on EC 1.1.3.7 - aryl-alcohol oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic tree
DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic tree, recombinant expression in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expression in Aspergillus nidulans
expression in Aspergillus nidulans, activity of the recombinant enzyme in Aspergillus nidulans culture is much higher than in extracellular fluid of Pleurotus eryngii
-
expression in Emericella nidulans under control of the fungal alc promoter
-
expression in Escherichia coli
expression in Pichia pastoris
expression of the constructs encoding CpSAO in Escherichia coli BL21(DE3) results in the formation of insoluble inclusion bodies. The expression construct is coexpressed with the plasmid pREP4-groESL, encoding chaperonine proteins of the Hsp60 family (GroEL and GroES). Coexpression at 15°C for 16 h results in the formation of soluble recombinant SAO
expression of the constructs encoding CtSAO in Escherichia coli BL21(DE3) results in the formation of insoluble inclusion bodies. The expression construct is coexpressed with the plasmid pREP4-groESL, encoding chaperonine proteins of the Hsp60 family (GroEL and GroES). Coexpression at 15°C for 16 h results in the formation of soluble recombinant SAO
expression of the mature enzyme in Escherichia coli
expression of wild-type and mutant enzymes in Emericella nidulans under control of the inducible fungal alc promoter
-
expression of wild-type enzyme in Escherichia coli, expression of wild-type and mutant enzymes in Emericella nidulans
-
gene aao, expression of FLAG1-tagged enzyme in Escherichia coli strain W3110, the non-glycosylated recombinant enzyme is successfully activated in vitro after Escherichia coli expression in form of inclusion bodies, expression iand glycosylation of the enzyme in Emericella nidulans strain IJFM A729
gene aao, expression of wild-type and mutant emzymes in Escherichia coli
gene aao, recombinant enzyme expression in Escherichia coli
gene aao, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae
-
gene aao, subcloning in Saccharomyces cerevisiae strain RH1385 for the plasmid construction via homologous recombination, heterologous recombinant expression in Coprinopsis cinerea strain FA2222 under control of the promotor of the glyceraldehyde-3-phosphate dehydrogenase gene 2 (gpdII) of the button mushroom Agaricus bisporus, and enzyme secretion to the culture medium. It might be that Coprinopsis cinerea does not provide the needed signal peptidase required for cleavage of the Pleurotus sapidus AAO signal peptide
gene gloA, recombinant expression in Aspergillus nidulans strain 773, method optimization using agitated and static cultures with or without pyridoxine, pyridoxine has a positive effect on enzyme production
-
in fusion with chimeric prepro-leader (pre-alpha-factor-proKiller) that enhances secretion by introducing the F[3alpha]S, N[25proK]D, T[50proK]A and F[52proK]L mutations
phylogenetic analysis, recombinant expression in Pichia pastoris
-