1.1.3.6: cholesterol oxidase
This is an abbreviated version!
For detailed information about cholesterol oxidase, go to the full flat file.
Word Map on EC 1.1.3.6
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1.1.3.6
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biosensors
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electrode
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esterase
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electrochemical
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brevibacterium
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fabric
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oxidases
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amperometric
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film
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rhodococcus
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raft
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cholestenone
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voltammetry
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cholest-4-en-3-one
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flavoenzyme
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cholesteryl
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nanocomposite
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biosensing
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erythropolis
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filipin
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4-cholesten-3-one
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nocardia
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medicine
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3hcholesterol
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electropolymerization
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lavendulae
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biotechnology
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agriculture
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co-immobilized
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methyl-beta-cyclodextrin
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diagnostics
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bioelectrode
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luminol
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screen-printed
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electrocatalytic
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analysis
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3.1.1.13
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polypyrrole
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multiwalled
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beta-ol
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synthesis
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drug development
- 1.1.3.6
-
biosensors
-
electrode
- esterase
-
electrochemical
- brevibacterium
-
fabric
- oxidases
-
amperometric
-
film
- rhodococcus
-
raft
- cholestenone
-
voltammetry
- cholest-4-en-3-one
-
flavoenzyme
-
cholesteryl
-
nanocomposite
-
biosensing
- erythropolis
- filipin
- 4-cholesten-3-one
- nocardia
- medicine
-
3hcholesterol
-
electropolymerization
- lavendulae
- biotechnology
- agriculture
-
co-immobilized
- methyl-beta-cyclodextrin
- diagnostics
-
bioelectrode
- luminol
-
screen-printed
-
electrocatalytic
- analysis
-
3.1.1.13
-
polypyrrole
-
multiwalled
- beta-ol
- synthesis
- drug development
Reaction
Synonyms
3beta-hydroxy steroid oxidoreductase, 3beta-hydroxysteroid: oxygen oxidoreductase, 3beta-hydroxysteroid:oxygen oxidoreductase, 3beta-hydroxysterol oxidase, BsChOx, CgChoA, CHO, CHO-U, ChO3, ChoA, choBb, CHOD, ChoG, ChoL, cholesterol oxidase, cholesterol oxidase I, cholesterol oxidase II, cholesterol-O2 oxidoreductase, CHOLOX, choM, ChoM1, ChoM2, choP, ChoRI, ChoRII, ChoS, ChOx, CO, CO1, COase, COD, COD-B, COX, HCEO-forming enzyme, HMPREF0204_11499, oxidase, cholesterol, PimE, ShChOx, type I ChOx
ECTree
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Purification
Purification on EC 1.1.3.6 - cholesterol oxidase
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butyl-Sepharose column chromatography and DEAE-cellulose column chromatography
effective method for the preparation of active PsChO3 using urea as denaturing agent, combined with his-tag trapping and on-column refolding. Using this method, the enzyme is successfully refolded from inclusion bodies expressed in Escherichia coli
extracellular enzyme 59fold from strain PKPD-CL by ammonium sulfate fractionation, anion exchange chromatography, ultrafiltration, and gel filtration
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native enzyme 2.3fold by ammnium sulfate fractionation and gel filtration
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native extracellular enzyme 14.3fold by ammonium sulfate fractionation and affinity chromatography
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native extracellular enzyme 18fold by anion exchange chromatography and gel filtration
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native extracellular enzyme 28.4fold from cell culture supernatant by ammonium sulfate fractionation, dialysis, and riboflavin affinity chromatography
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native extracellular enzyme from medium 12fold to homogeneity by ammonium sulfate fractionation, dialysis, ultrafiltration, anion exchange chromatography and gel filtration
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polyethylene glycol 4000 precipitation, diethylaminoethyl Sephacel anionic column chromatography and Superdex-200 gel filtration
Streptomyces sp. AKHSS
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recombinant His-tagged isozyme CHOM1 from Bacillus subtilis strain 168/pMA5 by nickel affinity chromatography
recombinant His-tagged isozyme CHOM2 from Bacillus subtilis strain 168/pMA5 by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli train BL21(DE3) by nickel affinity chromatography, dialysis and ultrafiltration
recombinant N-terminally His-tagged enzyme from Escherichia coli strain JM109 by nickel affinity chromatography
recombinant wild-type and mutant enzymes with with His-tags both at the N-terminus and at the C-terminus from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
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strain ST-200, ammonium sulfate, DEAE-cellulose, butyl-toyopearl, Sephadex G-100
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to purify the recombinant enzyme, 500 mg cholesterol is added to 50 ml of crude extract, and then incubated for 2 h at 4°C followed by centrifugation at 10000g for 10 min