1.1.3.6: cholesterol oxidase
This is an abbreviated version!
For detailed information about cholesterol oxidase, go to the full flat file.
Word Map on EC 1.1.3.6
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1.1.3.6
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biosensors
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electrode
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esterase
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electrochemical
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brevibacterium
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fabric
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oxidases
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amperometric
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film
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rhodococcus
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raft
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cholestenone
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voltammetry
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cholest-4-en-3-one
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flavoenzyme
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cholesteryl
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nanocomposite
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biosensing
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erythropolis
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filipin
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4-cholesten-3-one
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nocardia
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medicine
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3hcholesterol
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electropolymerization
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lavendulae
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biotechnology
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agriculture
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co-immobilized
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methyl-beta-cyclodextrin
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diagnostics
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bioelectrode
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luminol
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screen-printed
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electrocatalytic
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analysis
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3.1.1.13
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polypyrrole
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multiwalled
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beta-ol
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synthesis
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drug development
- 1.1.3.6
-
biosensors
-
electrode
- esterase
-
electrochemical
- brevibacterium
-
fabric
- oxidases
-
amperometric
-
film
- rhodococcus
-
raft
- cholestenone
-
voltammetry
- cholest-4-en-3-one
-
flavoenzyme
-
cholesteryl
-
nanocomposite
-
biosensing
- erythropolis
- filipin
- 4-cholesten-3-one
- nocardia
- medicine
-
3hcholesterol
-
electropolymerization
- lavendulae
- biotechnology
- agriculture
-
co-immobilized
- methyl-beta-cyclodextrin
- diagnostics
-
bioelectrode
- luminol
-
screen-printed
-
electrocatalytic
- analysis
-
3.1.1.13
-
polypyrrole
-
multiwalled
- beta-ol
- synthesis
- drug development
Reaction
Synonyms
3beta-hydroxy steroid oxidoreductase, 3beta-hydroxysteroid: oxygen oxidoreductase, 3beta-hydroxysteroid:oxygen oxidoreductase, 3beta-hydroxysterol oxidase, BsChOx, CgChoA, CHO, CHO-U, ChO3, ChoA, choBb, CHOD, ChoG, ChoL, cholesterol oxidase, cholesterol oxidase I, cholesterol oxidase II, cholesterol-O2 oxidoreductase, CHOLOX, choM, ChoM1, ChoM2, choP, ChoRI, ChoRII, ChoS, ChOx, CO, CO1, COase, COD, COD-B, COX, HCEO-forming enzyme, HMPREF0204_11499, oxidase, cholesterol, PimE, ShChOx, type I ChOx
ECTree
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Organic Solvent Stability
Organic Solvent Stability on EC 1.1.3.6 - cholesterol oxidase
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Acetone
benzene
Butanol
chloroform
cyclohexane
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119% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking
cyclooctane
dimethyl sulfoxide
DMSO
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Emal 20CM
Ethanol
Ethyl acetate
isopropanol
Methanol
p-xylene
propan-2-ol
the activity of the enzyme is rapidly inactivated in the presence of 30% (v/v) propan-2-ol at 25°C
sarcosyl
stable and partially or slightly activated in the presence of 0.5% (w/v) detergent
SDS
the enzyme is relatively tolerant to treatment with SDS after incubation for 1 h at 30°C
sodium cholate
sodium dodecyl sarcosinate
the enzyme is relatively tolerant to treatment with sodium dodecyl sarcosinate even at 60°C
sodium laurylbenzenesulfonate
the enzyme is relatively tolerant to treatment with sodium laurylbenzenesulfonate after incubation for 1 h at 30°C
sodium polyoxyethylene alkyl ether sulfate
toluene
Triton X-100
Triton X-405
Tween 20
urea
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the apoprotein of the mutant H69A lacks the characteristic tertiary structure of the holoprotein and displays larger hydrophobic surfaces. Its urea-induced unfolding does not occur by a simple two-state mechanism and is largely nonreversible. Minor alterations in the flavin binding region are evident between the native and the refolded proteins, and are likely responsible for the low refolding yield observed
xylene
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93% the activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking
additional information
Acetone
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Acetone
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37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, 90% loss of activity
Acetone
in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 2% activity remaining
Acetone
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in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 2% activity remaining
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127% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking
benzene
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37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable
Butanol
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Butanol
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37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable
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96% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking
chloroform
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37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable
chloroform
in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 89% activity remaining
chloroform
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in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 89% activity remaining
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37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable
cyclooctane
in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 108% activity remaining
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88% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking
dimethyl sulfoxide
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37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable
the enzyme is relatively tolerant to treatment with Emal 20CM even at 60°C
Emal 20CM
stable and partially or slightly activated in the presence of 0.5% (w/v) detergent
Emal 20CM
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stable and partially or slightly activated in the presence of 0.5% (w/v) detergent
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Ethanol
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Ethanol
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37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable
Ethanol
in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 31% activity remaining
Ethanol
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in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 31% activity remaining
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93% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking
Ethyl acetate
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93% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking
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Ethyl acetate
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Ethyl acetate
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37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
isopropanol
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37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable
isopropanol
in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 6% activity remaining
Methanol
the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C
Methanol
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37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable
Methanol
in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 65% activity remaining
Methanol
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in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 65% activity remaining
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37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable
p-xylene
in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 102% activity remaining
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the enzyme is stable at 50% (v/v) sodium cholate after incubation for 1 h at 30°C
sodium cholate
the enzyme is stable in sodium cholate after incubation for 1 h at 30°C
sodium cholate
stable and partially or slightly activated in the presence of 0.5% (w/v) detergent
sodium cholate
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stable and partially or slightly activated in the presence of 0.5% (w/v) detergent
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sodium cholate
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the enzyme retains more than 80% of its original activity in 0.5% (v/v) sodium cholate after incubation for 1 h at 60°C
sodium cholate
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the enzyme retains more than 80% of its original activity in 0.5% (v/v) sodium cholate after incubation for 1 h at 60°C
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the enzyme is stable at 50% (v/v) sodium polyoxyethylene alkyl ether sulfate after incubation for 1 h at 30°C
sodium polyoxyethylene alkyl ether sulfate
the enzyme is stable in sodium polyoxyethylene alkyl ether sulfate after incubation for 1 h at 30°C
toluene
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37°C, 24 h, 1 ml organic solvent added to 2 ml culture filtrate, stable
toluene
in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 112% activity remaining
toluene
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in a ratio 1:2 with enzyme solution, pH 7.0, 37°C, 24 h, 112% activity remaining
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the enzyme is stable at 50% (v/v) Triton X-100 after incubation for 1 h at 30°C
Triton X-100
the enzyme is stable in Triton X-100 after incubation for 1 h at 30°C
Triton X-100
stable and partially or slightly activated in the presence of 0.5% (w/v) detergent
Triton X-100
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stable and partially or slightly activated in the presence of 0.5% (w/v) detergent
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the enzyme is stable at 50% (v/v) Triton X-405 after incubation for 1 h at 30°C
Triton X-405
the enzyme is stable in Triton X-405 after incubation for 1 h at 30°C
Triton X-405
stable and partially or slightly activated in the presence of 0.5% (w/v) detergent
Triton X-405
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stable and partially or slightly activated in the presence of 0.5% (w/v) detergent
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Triton X-405
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the enzyme retains more than 80% of its original activity in 0.5% (v/v) Triton X-405 after incubation for 1 h at 60°C
Triton X-405
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the enzyme retains more than 80% of its original activity in 0.5% (v/v) Triton X-405 after incubation for 1 h at 60°C
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the enzyme is stable at 50% (v/v) Tween 20 after incubation for 1 h at 30°C
Tween 20
the enzyme is stable in Tween 20 after incubation for 1 h at 30°C
Tween 20
stable and partially or slightly activated in the presence of 0.5% (w/v) detergent
Tween 20
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stable and partially or slightly activated in the presence of 0.5% (w/v) detergent
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the enzyme shows moderate stability towards all organic solvents except acetone, benzene and chloroform. The activity increases in presence of isopropanol and ethanol
additional information
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the enzyme shows moderate stability towards all organic solvents except acetone, benzene and chloroform. The activity increases in presence of isopropanol and ethanol
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