1.1.3.6: cholesterol oxidase
This is an abbreviated version!
For detailed information about cholesterol oxidase, go to the full flat file.
Word Map on EC 1.1.3.6
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1.1.3.6
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biosensors
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electrode
-
esterase
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electrochemical
-
brevibacterium
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fabric
-
oxidases
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amperometric
-
film
-
rhodococcus
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raft
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cholestenone
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voltammetry
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cholest-4-en-3-one
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flavoenzyme
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cholesteryl
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nanocomposite
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biosensing
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erythropolis
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filipin
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4-cholesten-3-one
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nocardia
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medicine
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3hcholesterol
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electropolymerization
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lavendulae
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biotechnology
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agriculture
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co-immobilized
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methyl-beta-cyclodextrin
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diagnostics
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bioelectrode
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luminol
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screen-printed
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electrocatalytic
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analysis
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3.1.1.13
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polypyrrole
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multiwalled
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beta-ol
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synthesis
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drug development
- 1.1.3.6
-
biosensors
-
electrode
- esterase
-
electrochemical
- brevibacterium
-
fabric
- oxidases
-
amperometric
-
film
- rhodococcus
-
raft
- cholestenone
-
voltammetry
- cholest-4-en-3-one
-
flavoenzyme
-
cholesteryl
-
nanocomposite
-
biosensing
- erythropolis
- filipin
- 4-cholesten-3-one
- nocardia
- medicine
-
3hcholesterol
-
electropolymerization
- lavendulae
- biotechnology
- agriculture
-
co-immobilized
- methyl-beta-cyclodextrin
- diagnostics
-
bioelectrode
- luminol
-
screen-printed
-
electrocatalytic
- analysis
-
3.1.1.13
-
polypyrrole
-
multiwalled
- beta-ol
- synthesis
- drug development
Reaction
Synonyms
3beta-hydroxy steroid oxidoreductase, 3beta-hydroxysteroid: oxygen oxidoreductase, 3beta-hydroxysteroid:oxygen oxidoreductase, 3beta-hydroxysterol oxidase, BsChOx, CgChoA, CHO, CHO-U, ChO3, ChoA, choBb, CHOD, ChoG, ChoL, cholesterol oxidase, cholesterol oxidase I, cholesterol oxidase II, cholesterol-O2 oxidoreductase, CHOLOX, choM, ChoM1, ChoM2, choP, ChoRI, ChoRII, ChoS, ChOx, CO, CO1, COase, COD, COD-B, COX, HCEO-forming enzyme, HMPREF0204_11499, oxidase, cholesterol, PimE, ShChOx, type I ChOx
ECTree
1.1.3.6 cholesterol oxidaseAdvanced search results
results (
results (KM Value
KM Value on EC 1.1.3.6 - cholesterol oxidase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.3
3beta-hydroxy-androst-5-en-17-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.5
3beta-hydroxy-androst-5-en-17-one
-
assay relies on detection of H2O2 formation
0.8
3beta-hydroxy-androst-5-en-17-one
-
assay relies on detection of H2O2 formation
1.2
3beta-hydroxy-androst-5-en-17-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.27
5-Cholesten-3-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
1.52
5-Cholesten-3-one
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.17842
beta-sitosterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
0.18
beta-sitosterol
pH 7.5, 37°C, full-length isozyme ChoM2
0.19
beta-sitosterol
pH 7.5, 37°C, truncated isozyme ChoM2
0.22076
beta-sitosterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
0.32861
beta-sitosterol
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
0.2
cholestanol
-
substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.7
cholestanol
-
substrate cholestanol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.0015
cholesterol
mutant enzyme G347N, determined by cholest-4-en-3-one detection
0.0027
cholesterol
mutant enzyme F359W, determined by cholest-4-en-3-one detection
0.0027
cholesterol
wild-type enzyme, determined by cholest-4-en-3-one detection
0.0027
cholesterol
apparent value, wild type enzyme, pH 7.0, temperature not specified in the publication
0.0046
cholesterol
apparent value, mutant enzyme N485L, pH 7.0, temperature not specified in the publication
0.0062
cholesterol
apparent value, mutant enzyme N485L, pH 5.1, temperature not specified in the publication
0.0063
cholesterol
apparent value, mutant enzyme N485D, pH 5.1, temperature not specified in the publication
0.0066
cholesterol
apparent value, mutant enzyme N485D, pH 7.0, temperature not specified in the publication
0.0067
cholesterol
apparent value, wild type enzyme, pH 5.1, temperature not specified in the publication
0.007
cholesterol
mutant enzyme N485D, determined by cholest-4-en-3-one detection
0.01
cholesterol
pH 7.0, 37°C, mutant enzyme S153C/A205C/S312C/T435C, cholesterol solubilized in detergent micelles as a substrate
0.011
cholesterol
pH 7.0, 37°C, mutant enzyme A184C/A301C/T394C, cholesterol solubilized in detergent micelles as a substrate
0.011
cholesterol
pH 7.0, 37°C, mutant enzyme A32C/S129C/T371C/A423C, cholesterol solubilized in detergent micelles as a substrate
0.011
cholesterol
pH 7.0, 37°C, mutant enzyme L80C, cholesterol solubilized in detergent micelles as a substrate
0.012
cholesterol
pH 7.0, 37°C, mutant enzyme A32C/T168C/S312C/A465C, cholesterol solubilized in detergent micelles as a substrate
0.013
cholesterol
Nocardia erythropolis
-
Km increases when enzyme is entrapped in reverse micelles of the synthetic surfactant aerosol-OT-isooctane
0.013
cholesterol
pH 7.0, 37°C, mutant enzyme A184C/T239C/A407C/A465C, cholesterol solubilized in detergent micelles as a substrate
0.013
cholesterol
pH 7.0, 37°C, wild-type enzyme, cholesterol solubilized in detergent micelles as a substrate
0.014
cholesterol
pH 7.0, 37°C, mutant enzyme T168C/A276C, cholesterol solubilized in detergent micelles as a substrate
0.017
cholesterol
pH 7.0, 37°C, mutant enzyme L274C, cholesterol solubilized in detergent micelles as a substrate
0.0188
cholesterol
pH and temperature not specified in the publication
0.0262
cholesterol
pH and temperature not specified in the publication
0.04
cholesterol
-
substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.07
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.0962
cholesterol
-
enzyme from cultured immobilized cells, pH and temperature not specified in the publication
0.1013
cholesterol
-
enzyme from cultured free cells, pH and temperature not specified in the publication
0.11
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
0.12
cholesterol
pH 7.5, 37°C, full-length isozyme ChoM2
0.13
cholesterol
pH 7.5, 40°C, recombinant isozyme ChoM1
0.14
cholesterol
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.14
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
0.14
cholesterol
pH 7.5, 37°C, truncated isozyme ChoM2
0.15483
cholesterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
0.16
cholesterol
pH 7.5, 40°C, recombinant isozyme ChoM1
0.17
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
0.17831
cholesterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
0.2
cholesterol
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.2
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
0.2
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
0.20485
cholesterol
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
0.2123
cholesterol
-
apparent value, free enzyme, in 50 mM potassium phosphate buffer, pH 7.5, 30°C
0.25
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 0.5 M potassium phosphate, 1% thesit (polyoxyethylene(9)-lauryl-ether), 1.25% propan-2-ol, 25°C and pH 7.5
0.25
cholesterol
-
substrate cholesterol, polarographic determination of the rate of oxygen consumption. 1% thesit (polyoxyethylene(9)-lauryl-ether), 10% propan-2-ol and 50 mM phosphate, 25°C and pH 7.5
0.2599
cholesterol
-
apparent value, silk mat-immobilized enzyme, in 50 mM potassium phosphate buffer, pH 7.5, 30°C
0.4
cholesterol
-
substrate cholesterol, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.47
cholesterol
-
H69A mutant enzyme, 50 mM phosphate buffer, pH 7.5, 1% thesit, 1% 2-propanol
0.8
cholesterol
-
substrate cholesterol, detection of product formation (cholest-4-en-3-one) at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
35.4
cholesterol
-
N-terminally His-tagged recombinant enzyme , pH 7.2, 37°C
45.2
cholesterol
-
C-terminally His-tagged recombinant enzyme , pH 7.2, 37°C
0.2
dehydroandrosterone
pH 7.5, 37°C, full-length isozyme ChoM2
0.25
dehydroandrosterone
pH 7.5, 37°C, truncated isozyme ChoM2
0.27352
dehydroepiandrosterone
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
0.31881
dehydroepiandrosterone
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
0.34121
dehydroepiandrosterone
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
0.23
diosgenin
pH 7.5, 37°C, full-length isozyme ChoM2
0.26
diosgenin
pH 7.5, 37°C, truncated isozyme ChoM2
0.45
O2
-
H69A mutant enzyme, 50 mM phosphate buffer, pH 7.5, 1% thesit, 1% 2-propanol
0.19061
pregnenolone
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
0.2
pregnenolone
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.2
pregnenolone
-
substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.2
pregnenolone
-
substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.2
pregnenolone
-
substrate pregnenolone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.22842
pregnenolone
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
0.29455
pregnenolone
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
0.33
pregnenolone
pH 7.5, 37°C, full-length isozyme ChoM2
0.35
pregnenolone
pH 7.5, 37°C, truncated isozyme ChoM2
0.4
pregnenolone
-
assay relies on spectroscopic detection of 4-cholesten-3-one formation
0.4
pregnenolone
-
substrate pregnenolone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.38412
stigmasterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme F70V
0.52884
stigmasterol
-
pH 7.5, temperature not specified in the publication, mutant enzyme V64C
0.78961
stigmasterol
-
pH 7.5, temperature not specified in the publication, wild-type enzyme
0.5
trans-androsterone
-
substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.8
trans-androsterone
-
substrate trans-androsterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.2
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.3
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
0.9
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, rate of H2O2 formation detected with o-dianisidine and horseradish peroxidase. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
1.2
trans-dehydroandrosterone
-
substrate trans-dehydroandrosterone, detection of product formation at 240 nm. 0.1 M potassium phosphate, 1% Triton X-100, 1.25% propan-2-ol, 25°C and pH 7.5
additional information
additional information
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Km-values of enzyme immobilized on platinum electrode modified with thiolipid/lipid bilayer membranes
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additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
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additional information
additional information
Michaelis-Menten kinetics
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additional information
additional information
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Michaelis-Menten kinetics
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