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1.1.3.6: cholesterol oxidase

This is an abbreviated version!
For detailed information about cholesterol oxidase, go to the full flat file.

Word Map on EC 1.1.3.6

Reaction

cholesterol
+
O2
=
cholest-5-en-3-one
+
H2O2

Synonyms

3beta-hydroxy steroid oxidoreductase, 3beta-hydroxysteroid: oxygen oxidoreductase, 3beta-hydroxysteroid:oxygen oxidoreductase, 3beta-hydroxysterol oxidase, BsChOx, CgChoA, CHO, CHO-U, ChO3, ChoA, choBb, CHOD, ChoG, ChoL, cholesterol oxidase, cholesterol oxidase I, cholesterol oxidase II, cholesterol-O2 oxidoreductase, CHOLOX, choM, ChoM1, ChoM2, choP, ChoRI, ChoRII, ChoS, ChOx, CO, CO1, COase, COD, COD-B, COX, HCEO-forming enzyme, HMPREF0204_11499, oxidase, cholesterol, PimE, ShChOx, type I ChOx

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.6 cholesterol oxidase

Inhibitors

Inhibitors on EC 1.1.3.6 - cholesterol oxidase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-Fluoro-2,4-dinitrobenzene
-
0.1 mM, 71% inhibition
2-mercaptoethanol
2-pyridylhydroxymethane sulfate
4-chloromercuribenzoate
-
85% inhibition at 0.1 mM
5,10-Seco-19-nor-5-cholestyn-3,10-dione
Nocardia erythropolis
-
-
5alpha-Cholestan-3-one
-
-
5alpha-Cholestan-3beta,5alpha-diol
-
-
5alpha-Cholestan-3beta-ol
-
-
5alpha-Lanosta-8,24-dien-3beta-ol
-
-
5beta-Cholestan-3beta-ol
-
-
8-hydroxyquinoline
acetone
Ag2+
-
5 mM, 45% loss of activity
Benzene
beta-mercaptoethanol
-
1%, 40% loss of activity
butanol
chloroform
cholest-4-en-3-one
Nocardia rhodochrous
-
-
cholesterol
-
substrate inhibition of mutant V191A
CoCl2
-
1 mM, 30 min at 4°C, 21% inhibition
CuCl2
-
1 mM, 30 min at 4°C, 29% inhibition
CuSO4
Dimethylsulfoxide
dithiothreitol
-
-
ethyl acetate
FeCl3
fenpropimorph
FeSO4
HgCl2
Iodine
-
0.1 mM, 91% inhibition
iodoacetic acid
-
1 mM, 30 min at 4°C, 15% inhibition
laurylbenzenesulfonate
0.5% (w/v), complete inactivation; 0.5% (w/v), complete inactivation
-
Li+
-
8% inhibition at 0.1 mM
MgCl2
Mn2+
-
inhibits 4.5% at 5 mM
MnCl2
-
1 mM, 30 min at 4°C, 35% inhibition
N-bromosuccinimide
-
0.01 mM, 100% inhibition
Na+
-
slight inhibition at 1 mM
NaCl
-
80% residual activity with 20% NaCl (3.42 M) and 83% relative activity with 12% NaCl (2.05 M) concentration
NEM
-
1 mM, 30 min at 4°C, 24% inhibition
o-phenanthroline
p-chloromercuribenzoate
p-chloromercuribenzoic acid
-
the addition of p-chloromercuribenzoic acid partially reduces the activity of the enzyme
Pb(OAc)2
-
1 mM, complete inhibition
PCMB
-
cholesterol oxidase II
potassium cyanide
propan-2-ol
-
at 10% propan-2-ol kcat reaches 65% of that at 1.25% propan-2-ol
riboflavin
-
1 mM, 30 min at 4°C, 20% inhibition
rotenone
-
1 mM, 30 min at 4°C, 12% inhibition
Sarcosyl
sodium deoxycholate
-
-
Sodium dodecyl sulfate
-
inactivates the enzyme
sodium laurylbenzenesulfonate
complete inhibition
sodium polyoxyethylene alkyl ether sulfate
-
-
sulfhydryl reagents
-
-
Trinitrobenzene sulfonate
-
0.3 mM, 66% inhibition
Triton X-100
additional information
-