1.1.3.4: glucose oxidase
This is an abbreviated version!
For detailed information about glucose oxidase, go to the full flat file.
Word Map on EC 1.1.3.4
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1.1.3.4
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electrode
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biosensors
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electrochemical
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fabric
-
amperometric
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nanoparticles
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film
-
gold
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horseradish
-
biosensing
-
voltammetry
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nanotube
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niger
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electrocatalytic
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biocompatibility
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glassy
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gluconic
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entrap
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hydrogel
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platinum
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nanostructures
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layer-by-layer
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graphene
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electroactive
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nanocomposite
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electrochemistry
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multiwalled
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immunosensors
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co-immobilized
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sol-gel
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polypyrrole
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mesoporous
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prussian
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peroxidase-like
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nafion
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electrodeposition
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ferrocene
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bioelectrocatalytic
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screen-printed
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synthesis
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biocomposite
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diagnostics
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biotechnology
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polyaniline
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biofuel production
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industry
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pharmaceutical industry
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analysis
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metal-organic
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paper-based
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nanozymes
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food industry
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nanosheets
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energy production
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microa
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agriculture
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glucose-responsive
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3,3',5,5'-tetramethylbenzidine
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as-prepared
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photoelectrochemical
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medicine
- 1.1.3.4
-
electrode
-
biosensors
-
electrochemical
-
fabric
-
amperometric
- nanoparticles
-
film
- gold
- horseradish
-
biosensing
-
voltammetry
-
nanotube
- niger
-
electrocatalytic
-
biocompatibility
-
glassy
-
gluconic
-
entrap
- hydrogel
- platinum
-
nanostructures
-
layer-by-layer
-
graphene
-
electroactive
-
nanocomposite
-
electrochemistry
-
multiwalled
-
immunosensors
-
co-immobilized
-
sol-gel
-
polypyrrole
-
mesoporous
-
prussian
-
peroxidase-like
-
nafion
-
electrodeposition
- ferrocene
-
bioelectrocatalytic
-
screen-printed
- synthesis
-
biocomposite
- diagnostics
- biotechnology
-
polyaniline
- biofuel production
- industry
- pharmaceutical industry
- analysis
-
metal-organic
-
paper-based
-
nanozymes
- food industry
-
nanosheets
- energy production
-
microa
- agriculture
-
glucose-responsive
- 3,3',5,5'-tetramethylbenzidine
-
as-prepared
-
photoelectrochemical
- medicine
Reaction
Synonyms
AldO, beta-D-glucose oxidase, beta-D-glucose oxygen-1-oxidoreductase, beta-D-glucose/oxygen 1-oxidoreductase, beta-D-glucose: oxygen 1-oxidoreductase, beta-D-glucose:O2 1-oxidoreductase, beta-D-glucose:O2-1-oxidoreductase, beta-D-glucose:oxygen 1-oxido-reductase, beta-D-glucose:oxygen 1-oxidoreductase, beta-D-glucose:oxygen oxidoreductase, beta-D-glucose:oxygen-1-oxidoreductase, beta-D-glucose:quinone oxidoreductase, CngoxA, corylophyline, D-glucose oxidase, D-glucose-1-oxidase, deoxin-1, glucose aerodehydrogenase, glucose oxyhydrase, glucose-1-oxidase, GO-2, GOD, GOX, GOxP5, microcid, More, notatin, oxidase, glucose, pen-GOx, penatin, yGOXpenag
ECTree
1.1.3.4 glucose oxidaseAdvanced search results
results (
results (pH Stability
pH Stability on EC 1.1.3.4 - glucose oxidase
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pH STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
10
11
-
at 4°C, 7 months, glycine/NaOH buffer, residual activity of the glycosylated enzyme: less than 1%, residual activity of the deglycosylated enzyme: less than 1%
389832
12
-
at 4°C, 7 months, glycine/NaOH buffer, residual activity of the glycosylated enzyme: less than 1%, residual activity of the deglycosylated enzyme: less than 1%
389832
2
-
acid-induced unfolding of both forms of enzyme, significant loss of FAD cofactor and secondary structure
654358
2.5
-
the BTL wild-type strain enzyme is slightly more stable than the commercially available enzyme
389831
3 - 11
-
10% relative activity after 30 min at pH 3.0, 40% relative activity after 30 min at pH 4.0, 50% relative activity after 30 min at pH 5.0, 55% relative activity after 30 min at pH 6.0, 100% activity after 30 min at pH 7.0, 95% relative activity after 30 min at pH 8.0, 75% relative activity after 30 min at pH 9.0, 70% relative activity after 30 min at pH 10.0, 55% relative activity after 30 min at pH 11.0
710858
3 - 9
4 - 9
purified recombinant enzyme, more than 80% of activity is retained after incubation for 1 h at pHs from 4.0 to 9.0 and the maximum activity remains at pH 5.0-8.0
743193
4.5 - 5.2
-
recombinant enzyme, exhibits more than 80% of the maximum activity
389835
4.5 - 6
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the enzyme shows a pH-dependent response to the high pressure homogenization treatment, with reduction or maintenance of activity at pH 4.5-6.0 and a remarkable activity increase (30-300%) at pH 6.5 at all tested temperatures. i.e. 15°C, 50°C and 75°C
743644
4.5 - 7.4
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the poly(methyl methacrylate)-bovine serum albumin particle-adsorbed GOx has a higher catalytic activity at pH 7.4, close to the physiological environment, in comparison with that at pH 4.5
699922
5 - 7
5 - 8
5.5
6.2 - 6.5
-
recombinant enzyme, exhibits more than 80% of the maximum activity
389835
additional information
10
-
the native enzyme shows partial unfolding but the deglycosylated form shows a compaction of conformation
654358
10
-
at 4°C, 7 months, glycine/NaOH buffer, residual activity of the glycosylated enzyme: less than 1%, residual activity of the deglycosylated enzyme: less than 1%
389832
3
-
the BTL wild-type strain enzyme is slightly more stable than the commercially available enzyme
389831
3
-
at 4°C, 7 months, glycine/HCl buffer, residual activity of the glycosylated enzyme: 34%, residual activity of the deglycosylated enzyme: 10%
389832
3 - 9
-
2 h, 25°C, cell debris, stable, no loss of activity in the range of pH 3.0-7.0, loss of 10% activity at pH 8.0-9.0
672763
4
-
both the BTL wild-type strain enzyme and the commercially available enzyme are stable for at least 48 h
389831
4
-
at 4°C, 7 months, glycine/HCl buffer, residual activity of the glycosylated enzyme: 58%, residual activity of the deglycosylated enzyme: 30%
389832
4
-
at 4°C, 7 months, acetate buffer, residual activity of the glycosylated enzyme: 65%, residual activity of the deglycosylated enzyme: 59%
389832
5
-
both the BTL wild-type strain enzyme and the commercially available enzyme are stable for at least 48 h
389831
5
deglycosylation has no marked effect on the stability of the enzyme above pH 5
727055
5
-
at 4°C, 7 months, acetate buffer, residual activity of the glycosylated enzyme: 81%, residual activity of the deglycosylated enzyme: 60%
389832
5
-
extremely stable, no loss of activity detected after 300 days at 4°C and 30°C
389833
5 - 7
-
recombinant enzyme, more than 90% of the residual activity retained after 72 h incubation at room temperature
389835
6
-
both the BTL wild-type strain enzyme and the commercially available enzyme are stable for at least 48 h
389831
6
-
at 4°C, 7 months, acetate buffer, residual activity of the glycosylated enzyme: 76%, residual activity of the deglycosylated enzyme: 70%
389832
6
-
at 4°C, 7 months, phosphate buffer, residual activity of the glycosylated enzyme: 80%, residual activity of the deglycosylated enzyme: 75%
389832
6
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at pH 6.0 and at 50°C, the half life of the enzyme is about 20 h
712533
7
-
above, the recombinant enzyme is slightly less stable than native and deglycosylated enzyme
389835
7
-
at 4°C, 7 months, Tris/HCl buffer, residual activity of the glycosylated enzyme: 71%, residual activity of the deglycosylated enzyme: 70%
389832
7
-
at 4°C, 7 months, phosphate buffer, residual activity of the glycosylated enzyme: 88%, residual activity of the deglycosylated enzyme: 87%
389832
8
-
at 4°C, 7 months, phosphate buffer, residual activity of the glycosylated enzyme: 7%, residual activity of the deglycosylated enzyme: 5%
389832
8
-
at 4°C, 7 months, TRIS/HCl buffer, residual activity of the glycosylated enzyme: 20%, residual activity of the deglycosylated enzyme: 16%
389832
9
at alkaline pH, particularly at and above pH 9.0, the glycosylated and the declycosylated enzyme are relatively unstable
727055
9
-
at 4°C, 7 months, glycine/NaOH buffer, residual activity of the glycosylated enzyme: less than 5%, residual activity of the deglycosylated enzyme: less than 5%
389832
9
-
at 4°C, 7 months, Tris/HCl buffer, residual activity of the glycosylated enzyme: less than 2%, residual activity of the deglycosylated enzyme: less than 2%
389832
additional information
the deglycosylated enzyme is less stable at low pH
727055
additional information
-
the deglycosylated enzyme is less stable at low pH
727055
additional information
-
comparison of soluble and immobilized enzyme
389791
