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1.1.3.17: choline oxidase

This is an abbreviated version!
For detailed information about choline oxidase, go to the full flat file.

Word Map on EC 1.1.3.17

Reaction

Betaine aldehyde
+
O2
+
H2O
=
betaine
+
H2O2

Synonyms

alkaliphilic choline oxidase, ANI01nite_22550, An_CodA, APChO-syn, CHO, choline oxidase, choline-oxygen 1-oxidoreductase, choline:oxygen 1-reductase, ChOx, ChOx protein, codA, COX

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.17 choline oxidase

Purification

Purification on EC 1.1.3.17 - choline oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 41fold by ammonium sulfate fractionation, gel filtation, anion exchange and hydroxyapatite chromatgraphy, and again gel filtration
-
purified with the bound flavin cofactor in the fully oxidized state
recombinant enzyme partially from stressed and non-stressed transgenic rice plant leaves by ammonium sulfate fractionation and desalting gel filtration
-
recombinant enzyme, expression in Escherichia coli
recombinant protein
recombinant protein, purified to homogeneity, gel filtration
recombinant wild-type and mutants from Escherichia coli strain Rosetta(DE3)pLysS
recombinant wild-type enzyme in oxidized state to homogeneity
-
wild-type and mutant protein, purified enzymes have 50-70% of the enzyme-bound flavin cofactor as an air-stable anionic flavosemiquinone, converts slowly to oxidized state by extensive dialysis at pH 6 and 4°C
wild-type and mutant variants