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(R)-2-hydroxybutanoate + 2,6-dichlorophenolindophenol
2-oxobutanoate + reduced 2,6-dichlorophenolindophenol
preferred substrate
-
-
?
(R)-lactate + 2,6-dichlorophenolindophenol
pyruvate + reduced 2,6-dichlorophenolindophenol
preferred substrate
-
-
?
(S)-lactate + O2
acetate + CO2 + H2O
(S)-lactate + O2
pyruvate + H2O2
2-hydroxy-3-methylvalerate + O2
?
-
A95G-mutant
-
-
?
2-hydroxybutanoate + bromopyruvate + ?
bromolactate + pyruvate + ?
-
transhydrogenation reaction
-
?
2-hydroxybutyrate + O2
?
-
wild-type and A95G-mutant
-
-
?
2-hydroxycaproate + O2
2-oxocaproate + H2O2
-
-
-
?
2-hydroxycaprylate + O2
2-oxocaprylate + H2O2
-
-
-
?
2-hydroxydodecanoate + O2
2-oxododecanoate + H2O2
-
-
-
?
2-hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
-
-
-
?
2-hydroxyisovalerate + O2
?
-
A95G-mutant
-
-
?
2-hydroxyoctanoate + 2,6-dichlorophenolindophenol
2-oxo-octanoate + reduced 2,6-dichlorophenolindophenol
-
-
-
r
2-hydroxyoctanoate + O2
2-oxooctanoate + H2O2
-
substrates for isoenzymes HAOX1, HAOX2, preferred substrate for isoenzyme HAOX3
-
?
2-hydroxypalmitate + O2
2-oxopalmitate + H2O2
2-hydroxyvalerate + O2
?
-
wild-type and A95G-mutant
-
-
?
2-mercaptoethanol-glyoxylate adduct + O2
?
-
-
-
-
r
an (S)-2-hydroxy carboxylate + O2
a 2-oxo carboxylate + H2O2
coenzyme A-glyoxylate adduct + O2
?
-
-
-
-
r
D-2 -hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
D-lactate + O2
pyruvate + H2O2
DL-2-hydroxy-3-butynoate + O2
2-oxo-3-butynoate + H2O2
-
good substrate, but inactivation after 25 turnovers
-
?
DL-2-hydroxy-3-heptynoate + O2
2-oxo-3-heptynoate + H2O2
-
86% of the activity compared to DL-2-hydroxybutyrate
-
?
DL-2-hydroxy-3-hexynoate + O2
2-oxo-3-hexynoate + H2O2
-
65% of the activity compared to DL-2-hydroxybutyrate
-
?
DL-2-hydroxy-3-octynoate + O2
2-oxo-3-octynoate + H2O2
-
70% of the activity compared to DL-2-hydroxybutyrate
-
?
DL-2-hydroxy-3-pentynoate + O2
2-oxo-3-pentynoate + H2O2
-
2fold higher activity compared to DL-2-hydroxybutyrate
-
?
DL-2-hydroxy-4-methylmercaptobutyrate + 2,6-dichlorophenolindophenol
2-oxo-4-methylmercaptobutyrate + reduced 2,6-dichlorophenolindophenol
-
good substrate for long chain oxidase, low activity for short chain oxidase
-
?
DL-2-hydroxy-4-methylthiobutanoic acid + 2,6-dichlorophenolindophenol
2-oxo-4-methylthiobutanoic acid + reduced 2,6-dichlorophenolindophenol
-
-
-
?
DL-2-hydroxybutyrate + 2,4-dinitrophenylhydrazine
2-oxobutyrate 2,4-dinitrophenylhydrazone + ?
-
low activity
-
?
DL-2-hydroxybutyrate + 2,6-dichlorophenolindophenol
2-oxobutyrate + reduced 2,6-dichlorophenolindophenol
DL-2-hydroxybutyrate + O2
2-oxobutyrate + H2O2
DL-2-hydroxycaproate + 2,6-dichlorophenolindophenol
2-oxocaproate + reduced 2,6-dichlorophenolindophenol
-
low activity for short chain oxidase, moderate activity for long chain oxidase
-
?
DL-2-hydroxycaproate + O2
2-oxocaproate + H2O2
DL-2-hydroxydecanoate + 2,6-dichlorophenolindophenol
2-oxodecanoate + reduced 2,6-dichlorophenolindophenol
-
good substrate for long chain oxidase, traces of activity for short chain oxidase
-
?
DL-2-hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
DL-2-hydroxyisovalerate + 2,4-dinitrophenylhydrazine
2-oxoisovalerate 2,4-dinitrophenylhydrazone + ?
-
very low activity
-
?
DL-2-hydroxyisovalerate + 2,6-dichlorophenolindophenol
2-oxoisovalerate + reduced 2,6-dichlorophenolindophenol
-
no activity for short chain oxidase, moderate activity for long chain oxidase
-
?
DL-2-hydroxyisovalerate + O2
2-oxoisovalerate + H2O2
DL-2-hydroxyoctanoate + 2,6-dichlorophenolindophenol
2-oxooctanoate + reduced 2,6-dichlorophenolindophenol
-
good substrate for long chain oxidase, no activity for short chain oxidase
-
?
DL-2-hydroxyvalerate + 2,6-dichlorophenolindophenol
2-oxovalerate + reduced 2,6-dichlorophenolindophenol
-
low activity for short chain oxidase, moderate activity for long chain oxidase
-
?
DL-2-hydroxyvalerate + O2
2-oxovalerate + H2O2
DL-3-chlorolactate + O2
3-chloropyruvate + H2O2
DL-3-indolelactate + 2,6-dichlorophenolindophenol
3-indolepyruvate + reduced 2,6-dichlorophenolindophenol
-
good substrate for long chain oxidase, no activity for short chain oxidase
-
?
DL-3-indolelactate + O2
3-indolepyruvate + H2O2
-
-
-
?
DL-3-methoxy-4-hydroxymandelate + 2,6-dichlorophenolindophenol
(3-methoxy-4-hydroxyphenyl)pyruvate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
DL-alpha-phenyllactate + O2
? + H2O2
-
-
-
-
r
DL-beta-phenyllactate + 2,6-dichlorophenolindophenol
phenylpyruvate + reduced 2,6-dichlorophenolindophenol
-
no activity for short chain oxidase
-
?
DL-glycerate + O2
?
-
-
-
-
r
DL-glycerate + O2
? + H2O2
-
-
-
?
DL-lactate + O2
pyruvate + H2O2
-
-
-
?
DL-mandelate + 2,6-dichlorophenolindophenol
oxo(phenyl)acetic acid + reduced 2,6-dichlorophenolindophenol
-
low activity
-
?
DL-mandelate + O2
phenylglyoxylic acid + H2O2
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
DL-methionine + O2
? + H2O2
-
-
-
?
DL-p-hydroxy-beta-phenyllactate + 2,6-dichlorophenolindophenol
(4-hydroxyphenyl)pyruvate + reduced 2,6-dichlorophenolindophenol
-
no activity for short chain oxidase
-
?
DL-p-hydroxymandelate + O2
?
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
DL-phenyllactate + O2
phenylpyruvate + H2O2
DL-vinylglycolate + O2
2-oxo-3-butenoic acid + H2O2
-
90% of the activity compared to DL-2-hydroxybutyrate
-
?
glycolate + 2,4-dinitrophenylhydrazine
glyoxylate 2,4-dinitrophenylhydrazone + ?
-
best substrate tested
-
?
glycolate + 2,6-dichlorophenolindophenol
glyoxylate + reduced 2,6-dichlorophenolindophenol
glycolate + acceptor
glyoxylate + reduced acceptor
glycolate + ferricyanide
glyoxylate + ferrocyanide
glycolate + O2
glyoxylate + H2O2
glycolate + phenazine methosulfate
glyoxylate + reduced phenazine methosulfate
-
-
-
?
glycolate + phenylhydrazine
glyoxylate + phenylhydrazone
-
-
-
?
glyoxalate + O2
oxalate + H2O2
-
-
-
-
?
glyoxylate + 2,6-dichlorophenolindophenol
?
-
-
-
-
?
glyoxylate + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
glyoxylate + ferricyanide
? + ferrocyanide
-
-
-
?
glyoxylate thiohemiacetals + O2
? + H2O2
-
possible natural substrates, i.e. glyoxylate thiohemiacetals of coemzyme A, D-phosphopantetheine, D-pantetheine, N-acetylcysteamine, 2-mercaptoethanol, DL-dihydrolipoate, propane-1,3-dithiol
-
?
homoserine + O2
? + H2O2
-
traces of activity
-
?
isoleucic acid + O2
? + H2O2
-
-
-
?
L-2-hydroxy octanoate + O2
2-oxo-octanoate + H2O2
-
-
-
?
L-2-hydroxy palmitate + O2
2-oxo-palmitate + H2O2
-
-
-
?
L-2-hydroxy-4-methylthiobutanoic acid + O2
3-(methylthio)propanoate + HCO3-
-
oxidative decarboxylation
-
?
L-2-hydroxy-beta-methylvalerate + 2,6-dichlorophenolindophenol
3-methyl-2-oxopentanoate + reduced 2,6-dichlorophenolindophenol
L-2-hydroxyisocaproate + 2,4-dinitrophenylhydrazine
2-oxoisocaproate 2,4-dinitrophenylhydrazone + ?
-
-
-
?
L-2-hydroxyisocaproate + 2,6-dichlorophenolindophenol
2-oxoisocaproate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-2-hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
L-2-hydroxyisocaproate + O2
4-methyl-2-oxopentanoate + H2O2
-
100% activity with long chain oxidase, 32% activity with short chain oxidase
-
-
r
L-2-hydroxyphenyllactate + O2
? + H2O2
L-4-chloromandelate + O2
?
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
L-4-fluoromandelate + O2
?
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
L-4-methoxymandelate + O2
?
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
L-4-methylmandelate + O2
?
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
L-4-nitromandelate + O2
?
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
L-4-trifluoromethylmandelate + O2
?
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
L-alanine + O2
? + H2O2
-
low activity
-
?
L-alpha-hydroxy-isovalerate + O2
3-methyl-2-oxobutanoate + H2O2
-
-
-
-
r
L-alpha-hydroxyphenyllactate + O2
? + H2O2
-
-
-
-
r
L-isoleucine + O2
? + H2O2
-
low activity
-
?
L-lactate + 2,4-dinitrophenylhydrazine
pyruvate 2,4-dinitrophenylhydrazone + ?
-
very low activity
-
?
L-lactate + 2,6-dichlorophenolindophenol
pyruvate + reduced 2,6-dichlorophenolindophenol
L-lactate + O2
pyruvate + H2O2
L-lactate + phenazine methosulfate
? + reduced phenazine methosulfate
-
-
-
?
L-lactate + phenylhydrazine
pyruvate + phenylhydrazone
-
-
-
?
L-leucine + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-lysine + O2
? + H2O2
-
-
-
?
L-mandelate + 2,6-dichlorophenolindophenol
?
-
-
-
-
?
L-mandelate + 2,6-dichlorophenolindophenol
oxo(phenyl)acetic acid + reduced 2,6-dichlorophenolindophenol
L-mandelate + O2
? + H2O2
-
-
-
?
L-methionine + O2
? + H2O2
L-phenylalanine + O2
? + H2O2
-
-
-
?
L-phenyllactate + 2,6-dichlorophenolindophenol
phenylpyruvate + reduced 2,6-dichlorophenolindophenol
L-tryptophan + O2
? + H2O2
L-tyrosine + O2
? + H2O2
-
-
-
?
L-valine + O2
? + H2O2
-
low activity
-
?
lactate + O2
pyruvate + H2O2
mandelate + O2
phenylpyruvate + H2O2
oxidation of an L-2-hydroxy acid to a 2-oxoacid, model for the binding of L-mandelate into the active site, overview
-
-
?
N-acetylcysteamine-glyoxylate adduct + O2
?
-
-
-
-
r
pantetheine-glyoxylate adduct + O2
?
-
-
-
-
r
propane-1,3-dithiol-glyoxylate adduct + O2
?
-
-
-
-
r
thiol-glyoxylate adducts + O2
an oxalyl thioester + H2O2
-
may be the physiological substrates
-
?
valic acid + O2
? + H2O2
-
-
-
?
additional information
?
-
(S)-lactate + O2
acetate + CO2 + H2O
-
-
-
-
?
(S)-lactate + O2
acetate + CO2 + H2O
-
-
-
-
?
(S)-lactate + O2
acetate + CO2 + H2O
-
-
-
-
?
(S)-lactate + O2
acetate + CO2 + H2O
-
glucose-repressible lactate oxidase is likely responsible for H2O2 production
-
-
?
(S)-lactate + O2
pyruvate + H2O2
-
no decarboxylation
-
?
(S)-lactate + O2
pyruvate + H2O2
-
no decarboxylation
-
?
(S)-lactate + O2
pyruvate + H2O2
-
-
-
?
(S)-lactate + O2
pyruvate + H2O2
-
-
-
-
?
(S)-lactate + O2
pyruvate + H2O2
-
-
-
?
(S)-lactate + O2
pyruvate + H2O2
-
-
-
-
?
2-hydroxypalmitate + O2
2-oxopalmitate + H2O2
-
-
-
?
2-hydroxypalmitate + O2
2-oxopalmitate + H2O2
-
substrates for isoenzymes HAOX1, HAOX2
-
?
an (S)-2-hydroxy carboxylate + O2
a 2-oxo carboxylate + H2O2
-
-
-
?
an (S)-2-hydroxy carboxylate + O2
a 2-oxo carboxylate + H2O2
-
-
-
?
an (S)-2-hydroxy carboxylate + O2
a 2-oxo carboxylate + H2O2
-
-
-
?
an (S)-2-hydroxy carboxylate + O2
a 2-oxo carboxylate + H2O2
-
-
-
?
an (S)-2-hydroxy carboxylate + O2
a 2-oxo carboxylate + H2O2
-
-
-
?
an (S)-2-hydroxy carboxylate + O2
a 2-oxo carboxylate + H2O2
-
-
-
?
an (S)-2-hydroxy carboxylate + O2
a 2-oxo carboxylate + H2O2
-
-
-
?
an (S)-2-hydroxy carboxylate + O2
a 2-oxo carboxylate + H2O2
-
-
-
?
D-2 -hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
-
48% of the activity compared to glycolate
-
?
D-2 -hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
-
liver enzyme is much less active with C4 or C5 alpha-hydroxy acids but as active as with glycolate
-
?
D-lactate + O2
pyruvate + H2O2
-
traces of activity
-
?
D-lactate + O2
pyruvate + H2O2
-
traces of activity
-
?
D-lactate + O2
pyruvate + H2O2
-
traces of activity
-
?
DL-2-hydroxybutyrate + 2,6-dichlorophenolindophenol
2-oxobutyrate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
DL-2-hydroxybutyrate + 2,6-dichlorophenolindophenol
2-oxobutyrate + reduced 2,6-dichlorophenolindophenol
-
low activity for long chain oxidase, no activity for short chain oxidase
-
?
DL-2-hydroxybutyrate + O2
2-oxobutyrate + H2O2
-
68% of the activity compared to glycolate
-
?
DL-2-hydroxybutyrate + O2
2-oxobutyrate + H2O2
-
-
-
?
DL-2-hydroxybutyrate + O2
2-oxobutyrate + H2O2
-
-
-
?
DL-2-hydroxybutyrate + O2
2-oxobutyrate + H2O2
-
-
-
?
DL-2-hydroxybutyrate + O2
2-oxobutyrate + H2O2
-
-
-
?
DL-2-hydroxybutyrate + O2
2-oxobutyrate + H2O2
-
-
-
?
DL-2-hydroxybutyrate + O2
2-oxobutyrate + H2O2
-
-
-
?
DL-2-hydroxybutyrate + O2
2-oxobutyrate + H2O2
-
-
-
?
DL-2-hydroxybutyrate + O2
2-oxobutyrate + H2O2
-
best substrate tested
-
?
DL-2-hydroxybutyrate + O2
2-oxobutyrate + H2O2
-
higher affinity with C5 and C6 hydroxy acids
-
?
DL-2-hydroxybutyrate + O2
2-oxobutyrate + H2O2
-
best substrate tested
-
?
DL-2-hydroxycaproate + O2
2-oxocaproate + H2O2
-
-
-
?
DL-2-hydroxycaproate + O2
2-oxocaproate + H2O2
-
-
-
?
DL-2-hydroxycaproate + O2
2-oxocaproate + H2O2
-
-
-
?
DL-2-hydroxycaproate + O2
2-oxocaproate + H2O2
-
-
-
?
DL-2-hydroxycaproate + O2
2-oxocaproate + H2O2
-
-
-
?
DL-2-hydroxycaproate + O2
2-oxocaproate + H2O2
-
low activity
-
?
DL-2-hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
-
-
-
?
DL-2-hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
-
-
-
?
DL-2-hydroxyisovalerate + O2
2-oxoisovalerate + H2O2
-
-
-
?
DL-2-hydroxyisovalerate + O2
2-oxoisovalerate + H2O2
-
low activity
-
?
DL-2-hydroxyisovalerate + O2
2-oxoisovalerate + H2O2
-
low activity
-
?
DL-2-hydroxyisovalerate + O2
2-oxoisovalerate + H2O2
-
low activity
-
?
DL-2-hydroxyisovalerate + O2
2-oxoisovalerate + H2O2
-
low activity
-
?
DL-2-hydroxyvalerate + O2
2-oxovalerate + H2O2
-
39% of the activity compared to glycolate
-
?
DL-2-hydroxyvalerate + O2
2-oxovalerate + H2O2
-
-
-
?
DL-2-hydroxyvalerate + O2
2-oxovalerate + H2O2
-
-
-
?
DL-2-hydroxyvalerate + O2
2-oxovalerate + H2O2
-
-
-
?
DL-2-hydroxyvalerate + O2
2-oxovalerate + H2O2
-
-
-
?
DL-2-hydroxyvalerate + O2
2-oxovalerate + H2O2
-
-
-
?
DL-2-hydroxyvalerate + O2
2-oxovalerate + H2O2
-
low activity
-
?
DL-3-chlorolactate + O2
3-chloropyruvate + H2O2
-
-
-
?
DL-3-chlorolactate + O2
3-chloropyruvate + H2O2
-
-
-
?
DL-3-chlorolactate + O2
3-chloropyruvate + H2O2
-
best substrate tested
-
?
DL-phenyllactate + O2
phenylpyruvate + H2O2
-
-
-
?
DL-phenyllactate + O2
phenylpyruvate + H2O2
-
-
-
?
DL-phenyllactate + O2
phenylpyruvate + H2O2
-
-
-
?
glycerate + O2
? + H2O2
-
-
-
?
glycerate + O2
? + H2O2
-
-
-
-
?
glycolate + 2,6-dichlorophenolindophenol
glyoxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
glycolate + 2,6-dichlorophenolindophenol
glyoxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
glycolate + 2,6-dichlorophenolindophenol
glyoxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
glycolate + 2,6-dichlorophenolindophenol
glyoxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
glycolate + 2,6-dichlorophenolindophenol
glyoxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
glycolate + 2,6-dichlorophenolindophenol
glyoxylate + reduced 2,6-dichlorophenolindophenol
-
highest activity for short chain oxidase, no activity for long chain oxidase
-
?
glycolate + acceptor
glyoxylate + reduced acceptor
-
-
-
?
glycolate + acceptor
glyoxylate + reduced acceptor
-
-
-
-
?
glycolate + ferricyanide
glyoxylate + ferrocyanide
-
-
-
?
glycolate + ferricyanide
glyoxylate + ferrocyanide
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
highest activity for isoenzyme HAOX1, no activity for isoenzymes HAOX2, HAOX3
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
substrate for isoenzyme A
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
the catalytic adduct is formed by hydrogen abstraction from the re-face of glycolate
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
best substrate
-
-
?
glycolate + O2
glyoxylate + H2O2
plant
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
substrate for isoenzyme A
-
?
glycolate + O2
glyoxylate + H2O2
-
isoenzyme A utilizes short chain aliphatic hydroxy acids, isoenzyme B utilizes long-chain and aromatic hydroxyacids, that may also utilize L-amino acids
-
?
glycolate + O2
glyoxylate + H2O2
-
preference for long chain substrates, more efficient hydroxy acid oxidase than an amino acid oxidase
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
-
?
glycolate + O2
glyoxylate + H2O2
-
-
-
-
?
glyoxylate + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
glyoxylate + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
glyoxylate + O2
? + H2O2
-
substrate for isoenzyme HAOX1
-
?
glyoxylate + O2
? + H2O2
-
26% of the activity compared to glycolate
-
?
glyoxylate + O2
? + H2O2
-
-
-
?
glyoxylate + O2
? + H2O2
-
-
-
?
glyoxylate + O2
? + H2O2
-
in the absence of any nucleophile less than 2% of the activity compared to glycolate
-
?
glyoxylate + O2
? + H2O2
-
40% of the activity compared to glycolate
-
?
glyoxylate + O2
? + H2O2
-
-
-
?
L-2-hydroxy-beta-methylvalerate + 2,6-dichlorophenolindophenol
3-methyl-2-oxopentanoate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
L-2-hydroxy-beta-methylvalerate + 2,6-dichlorophenolindophenol
3-methyl-2-oxopentanoate + reduced 2,6-dichlorophenolindophenol
-
no activity for short chain oxidase
-
?
L-2-hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
-
substrate for isoenzyme B
-
?
L-2-hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
-
-
-
?
L-2-hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
-
-
-
?
L-2-hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
-
-
-
?
L-2-hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
-
best substrate tested
-
?
L-2-hydroxyisocaproate + O2
2-oxoisocaproate + H2O2
-
substrate for isoenzyme B
-
?
L-2-hydroxyphenyllactate + O2
? + H2O2
-
-
-
?
L-2-hydroxyphenyllactate + O2
? + H2O2
-
-
-
?
L-lactate + 2,6-dichlorophenolindophenol
pyruvate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
L-lactate + 2,6-dichlorophenolindophenol
pyruvate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-lactate + 2,6-dichlorophenolindophenol
pyruvate + reduced 2,6-dichlorophenolindophenol
-
lower activity for preparation from "heavy" mitochondria
-
?
L-lactate + 2,6-dichlorophenolindophenol
pyruvate + reduced 2,6-dichlorophenolindophenol
-
very low activity
-
?
L-lactate + O2
pyruvate + H2O2
-
-
-
-
?
L-lactate + O2
pyruvate + H2O2
-
-
-
?
L-lactate + O2
pyruvate + H2O2
-
28% of the activity compared to glycolate
-
?
L-lactate + O2
pyruvate + H2O2
-
-
-
?
L-lactate + O2
pyruvate + H2O2
-
yoghurt mixed and homogenized in water, filtered (20-25 microm), this sample injected into the luminometer measuring cell containing the lactate biosensor-system
-
-
?
L-lactate + O2
pyruvate + H2O2
-
-
-
?
L-lactate + O2
pyruvate + H2O2
-
-
-
?
L-lactate + O2
pyruvate + H2O2
-
-
-
?
L-lactate + O2
pyruvate + H2O2
-
-
-
?
L-lactate + O2
pyruvate + H2O2
-
very low activity
-
?
L-lactate + O2
pyruvate + H2O2
-
high activity, does not act on D-lactate
-
?
L-leucine + O2
? + H2O2
-
-
-
?
L-leucine + O2
? + H2O2
-
-
-
?
L-leucine + O2
? + H2O2
-
highest activity
-
?
L-mandelate + 2,6-dichlorophenolindophenol
oxo(phenyl)acetic acid + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-mandelate + 2,6-dichlorophenolindophenol
oxo(phenyl)acetic acid + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-mandelate + O2
?
-
A95G-mutant is also reactive
-
-
?
L-mandelate + O2
?
-
the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive
-
-
?
L-methionine + O2
? + H2O2
-
-
-
?
L-methionine + O2
? + H2O2
-
-
-
?
L-phenyllactate + 2,6-dichlorophenolindophenol
phenylpyruvate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-phenyllactate + 2,6-dichlorophenolindophenol
phenylpyruvate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
L-tryptophan + O2
? + H2O2
-
-
-
?
L-tryptophan + O2
? + H2O2
-
-
-
?
L-tryptophan + O2
? + H2O2
-
-
-
?
lactate + O2
pyruvate + H2O2
-
-
-
?
lactate + O2
pyruvate + H2O2
-
-
-
?
lactate + O2
pyruvate + H2O2
-
lactate detection in beer samples
H2O2 oxidizes Prussian Blue on an electrode, the concomitant electron flow is measured
-
?
lactate + O2
pyruvate + H2O2
-
-
-
?
lactate + O2
pyruvate + H2O2
-
-
-
?
additional information
?
-
isozyme lHAOX1 displays the highest activity with the long-chain fatty acid 2-hydroxyhexadecanoic acid (2-hydroxypalmitic acid) and has intermediate activity with 2-hydroxyhexanoic acid (2-hydroxycaproic acid), 2-hydroxyoctanoic acid (2-hydroxycaprylic acid), and the short-chain hydroxyacid L-lactate. With much lower activity, it can also use glycolate, leucic acid, valic acid, and isoleucic acid as substrates. No activity with 2-hydroxyhexadecanoic acid and D-lactate
-
-
?
additional information
?
-
isozyme lHAOX1 displays the highest activity with the long-chain fatty acid 2-hydroxyhexadecanoic acid (2-hydroxypalmitic acid) and has intermediate activity with 2-hydroxyhexanoic acid (2-hydroxycaproic acid), 2-hydroxyoctanoic acid (2-hydroxycaprylic acid), and the short-chain hydroxyacid L-lactate. With much lower activity, it can also use glycolate, leucic acid, valic acid, and isoleucic acid as substrates. No activity with 2-hydroxyhexadecanoic acid and D-lactate
-
-
?
additional information
?
-
isozyme lHAOX1 displays the highest activity with the long-chain fatty acid 2-hydroxyhexadecanoic acid (2-hydroxypalmitic acid) and has intermediate activity with 2-hydroxyhexanoic acid (2-hydroxycaproic acid), 2-hydroxyoctanoic acid (2-hydroxycaprylic acid), and the short-chain hydroxyacid L-lactate. With much lower activity, it can also use glycolate, leucic acid, valic acid, and isoleucic acid as substrates. No activity with 2-hydroxyhexadecanoic acid and D-lactate
-
-
?
additional information
?
-
isozyme lHAOX2 exhibits the highest activity with leucic acid. It shows intermediate activity with 2-hydroxyhexanoic acid and 2-hydroxyoctanoic acid. lHAOX2 displays lower activity with 2-hydroxydodecanoic acid, valic acid, and isoleucic acid and poor activity with glycolate and L-lactate. No activity with 2-hydroxyhexadecanoic acid and D-lactate
-
-
?
additional information
?
-
isozyme lHAOX2 exhibits the highest activity with leucic acid. It shows intermediate activity with 2-hydroxyhexanoic acid and 2-hydroxyoctanoic acid. lHAOX2 displays lower activity with 2-hydroxydodecanoic acid, valic acid, and isoleucic acid and poor activity with glycolate and L-lactate. No activity with 2-hydroxyhexadecanoic acid and D-lactate
-
-
?
additional information
?
-
isozyme lHAOX2 exhibits the highest activity with leucic acid. It shows intermediate activity with 2-hydroxyhexanoic acid and 2-hydroxyoctanoic acid. lHAOX2 displays lower activity with 2-hydroxydodecanoic acid, valic acid, and isoleucic acid and poor activity with glycolate and L-lactate. No activity with 2-hydroxyhexadecanoic acid and D-lactate
-
-
?
additional information
?
-
no substrates: D-alanine, pyruvate, D-glucose, L-alanine, ethanol and L-lactate
-
-
-
additional information
?
-
-
no substrate: oxalate, acetate, pyruvate, glycerol, propionate, succinate, fumarate, malate, maleate, tartrate, oxaloacetate, 2-oxoglutarate, glycocol,L-alanine, serine, glutamate, ascorbate, glucose, and fructose
-
-
?
additional information
?
-
-
no substrate: glyoxylate. Enzyme also catalyzes the oxidation of short-chain alcohols, reaction of EC 1.1.3.13
-
-
-
additional information
?
-
-
interaction of Rice dwarf virus, RDV, outer capsid P8 protein with rice glycolate oxidase mediates relocalization of P8, GOX may play important roles in RDV targeting into the replication site of host cells, overview
-
-
?
additional information
?
-
GLO is a typical photorespiratory enzyme and it exerts a strong regulation over photosynthesis, possibly through a feed-back inhibition on Rubisco activase, the glyoxylate cycle may be partially activated to compensate for the photorespiratory glyoxylate when GLO is suppressed in rice
-
-
?
additional information
?
-
-
GLO is a typical photorespiratory enzyme and it exerts a strong regulation over photosynthesis, possibly through a feed-back inhibition on Rubisco activase, the glyoxylate cycle may be partially activated to compensate for the photorespiratory glyoxylate when GLO is suppressed in rice
-
-
?
additional information
?
-
the enzyme is involved in the photorespiration process
-
-
?
additional information
?
-
the enzyme is not able to metabolize D-lactate
-
-
?
additional information
?
-
-
the enzyme is not able to metabolize D-lactate
-
-
?
additional information
?
-
long-chain L-alpha-hydroxy acid oxidase (LCHAO) is a FMN-dependent oxidase that dehydrogenates L-alpha-hydroxy acids to oxo acids
-
-
?
additional information
?
-
-
the enzyme is involved in the photorespiration process
-
-
?
additional information
?
-
-
energy-yielding metabolism can be described as follows: as long as glucose is available, approximatelyone-fourth of the pyruvate formed is converted to acetate by the sequential action of pyruvate oxidase and acetate kinase with acquisition of additional ATP. The rest of the pyruvate is reduced by lactate dehydrogenase to form lactate, with partial achievement of redox balance. The lactate is oxidized by lactate oxidase back to pyruvate, which is converted to acetate as described above; and the sequential reactions mentioned above continue to occur as long as lactate is present
-
-
?
additional information
?
-
-
energy-yielding metabolism can be described as follows: as long as glucose is available, approximatelyone-fourth of the pyruvate formed is converted to acetate by the sequential action of pyruvate oxidase and acetate kinase with acquisition of additional ATP. The rest of the pyruvate is reduced by lactate dehydrogenase to form lactate, with partial achievement of redox balance. The lactate is oxidized by lactate oxidase back to pyruvate, which is converted to acetate as described above; and the sequential reactions mentioned above continue to occur as long as lactate is present
-
-
?