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1.1.3.15: (S)-2-hydroxy-acid oxidase

This is an abbreviated version!
For detailed information about (S)-2-hydroxy-acid oxidase, go to the full flat file.

Word Map on EC 1.1.3.15

Reaction

an (S)-2-hydroxy carboxylate
+
O2
=
a 2-oxo carboxylate
+
H2O2

Synonyms

(L)-2-HAOX, (S)-2-hydroxy-acid oxidase, peroxisomal, 2-hydroxy acid oxidase, CSUB_C1080, EC 1.1.3.1, GLO, Glo1, GLO3, Glo4, glycolate oxidase, GO, GO1, GOX, GOX1, GOX2, HAO1, Hao2, HAOX, HAOX1, HAOX2, HAOX3, hydroxy-acid oxidase A, hydroxy-acid oxidase B, hydroxyacid oxidase 1, hydroxyacid oxidase A, L-2-hydroxy acid oxidase, L-2-hydroxyacid oxidase A, L-alpha-hydroxy acid oxidase, L-amino acid oxidase, L-LAC-OX, L-lactate monooxygenase, L-lactate oxidase, L-LOx, lactate oxidase, LCHAO, LctO, lHAOX1, lHAOX2, long chain hydroxy acid oxidase, long chain l-2-hydroxy acid oxidase, long chain L-2-hydroxy acid oxidase 2, long-chain 2-hydroxy acid oxidase, long-chain L-alpha-hydroxy acid oxidase, LOX, More, NbS00005125g0015, NbS00060838g0004, Nbv5tr6245008, Os03g0786100, Os04g0623500, Os07g0616500, oxidase, L-2-hydroxy acid, RCOM_0631490, RCOM_0684800

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.15 (S)-2-hydroxy-acid oxidase

Storage Stability

Storage Stability on EC 1.1.3.15 - (S)-2-hydroxy-acid oxidase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, ammonium sulfate precipitate or in solution with 2.5 M glycerol, several months
-
-20°C, lyophilized powder containing 70-80% sucrose, stable for more than a year
-
-5°C, 3.2 M ammonium sulfate, pH 8.3, 0.002 M FMN
-
-70°C, concentrated solution, stable for several months
-
4°C, 10 mM Tris-HCl, 15 mM NaCl, 0.02 mM FMN, 1 mM EDTA, several weeks, cannot be stored frozen at -20°C or -80°C
-
4°C, 60% ammonium sulfate precipitate, several months
-
below 0°C, 0.1 mol/l phosphate buffer, pH 7.2 (potassium and sodium salts), 2.2 mg lactate oxidase in 0.5 ml buffer, enzymatic activity stable for several weeks
-