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1.1.3.15: (S)-2-hydroxy-acid oxidase

This is an abbreviated version!
For detailed information about (S)-2-hydroxy-acid oxidase, go to the full flat file.

Word Map on EC 1.1.3.15

Reaction

an (S)-2-hydroxy carboxylate
+
O2
=
a 2-oxo carboxylate
+
H2O2

Synonyms

(L)-2-HAOX, (S)-2-hydroxy-acid oxidase, peroxisomal, 2-hydroxy acid oxidase, CSUB_C1080, EC 1.1.3.1, GLO, Glo1, GLO3, Glo4, glycolate oxidase, GO, GO1, GOX, GOX1, GOX2, HAO1, Hao2, HAOX, HAOX1, HAOX2, HAOX3, hydroxy-acid oxidase A, hydroxy-acid oxidase B, hydroxyacid oxidase 1, hydroxyacid oxidase A, L-2-hydroxy acid oxidase, L-2-hydroxyacid oxidase A, L-alpha-hydroxy acid oxidase, L-amino acid oxidase, L-LAC-OX, L-lactate monooxygenase, L-lactate oxidase, L-LOx, lactate oxidase, LCHAO, LctO, lHAOX1, lHAOX2, long chain hydroxy acid oxidase, long chain l-2-hydroxy acid oxidase, long chain L-2-hydroxy acid oxidase 2, long-chain 2-hydroxy acid oxidase, long-chain L-alpha-hydroxy acid oxidase, LOX, More, NbS00005125g0015, NbS00060838g0004, Nbv5tr6245008, Os03g0786100, Os04g0623500, Os07g0616500, oxidase, L-2-hydroxy acid, RCOM_0631490, RCOM_0684800

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.15 (S)-2-hydroxy-acid oxidase

Purification

Purification on EC 1.1.3.15 - (S)-2-hydroxy-acid oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
centrifugation, washing with 10 mM potassium phosphate buffer, pH 6.5, stored at -20°C, resuspended with same buffer, treated with lysozyme followed by disruption with glass beads in a bead mill, centrifugation, supernatants used for enzyme assays
native enzyme from leaves 207fold by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and again gel filtration
-
native enzyme from leaves to homogeneity
-
native enzyme from maize leaf bundle sheath 46.6fold by gel filtration, anion exchange chromatography, and again gel filtration. Native enzyme 10.5fold from leaf mesophyll, by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and again gel filtration
-
native enzyme, subcellular fractionation
-
near homogeneity, precipitation techniques
-
partial, isoenzymes A and B
-
purification via dye affinity chromatography
-
purified in one step on hydroxyapatite column onto which it is strongly adsorbed. For the initial purification steps, FMN is added to the buffers
-
recombinant enzyme
-
recombinant enzyme by nickel affinity chromatography, ammonium sulfate fractionation and dialysis
recombinant His-tagged enzyme from Escherichia coli strain BLR DE3 pLysS by nickel affinity chromatography and desalting gel filtration to homogeneity
recombinant His-tagged enzyme from Escherichia coli strain BLR(DE3) pLysS by nickel affinity chromatography and desalting gel filtration to homogeneity
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography to high purity
recombinant isoenzymes
-
recombinant isozymes beta1, beta2
-
recombinant protein
recombinant protein, Ni affinity chromatography
tissue-specific isozymes from leaves 63.5fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, bundle sheath cell isozyme and mesophyll cell isozyme to homogeneity
-
to crystalline state, chromatography and precipitation techniques
to crystalline state, precipitation techniques
-
to crystalline state, recombinant enzyme
-
to homogeneity
-
to homogeneity, 2-step chromatography
-
to homogeneity, chromatography steps
-
to homogeneity, chromatography techniques
-
to homogeneity, chromatography techniques, 2 distinct forms
-
using a Ni-NTA-column, gel filtration and ion-exchange chromatography