1.1.3.15: (S)-2-hydroxy-acid oxidase

This is an abbreviated version!
For detailed information about (S)-2-hydroxy-acid oxidase, go to the full flat file.

Reaction

Synonyms

(L)-2-HAOX, (S)-2-hydroxy-acid oxidase, peroxisomal, 2-hydroxy acid oxidase, CSUB_C1080, EC 1.1.3.1, GLO, Glo1, GLO3, Glo4, glycolate oxidase, GO, GO1, GOX, GOX1, GOX2, HAO1, Hao2, HAOX, HAOX1, HAOX2, HAOX3, hydroxy-acid oxidase A, hydroxy-acid oxidase B, hydroxyacid oxidase 1, hydroxyacid oxidase A, L-2-hydroxy acid oxidase, L-2-hydroxyacid oxidase A, L-alpha-hydroxy acid oxidase, L-amino acid oxidase, L-LAC-OX, L-lactate monooxygenase, L-lactate oxidase, L-LOx, lactate oxidase, LCHAO, LctO, lHAOX1, lHAOX2, long chain hydroxy acid oxidase, long chain l-2-hydroxy acid oxidase, long chain L-2-hydroxy acid oxidase 2, long-chain 2-hydroxy acid oxidase, long-chain L-alpha-hydroxy acid oxidase, LOX, More, NbS00005125g0015, NbS00060838g0004, Nbv5tr6245008, Os03g0786100, Os04g0623500, Os07g0616500, oxidase, L-2-hydroxy acid, RCOM_0631490, RCOM_0684800

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.3 With oxygen as acceptor

                1.1.3.15 (S)-2-hydroxy-acid oxidase

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Results	in table
8	Activating Compound
4085	AA Sequence
19	Application
1	CAS Registry Number
32	Cloned(Commentary)
85	Cofactor
13	Crystallization (Commentary)
183	Disease/ Diagnostics
13	Expression
38	General Information
5	General Stability
104	IC50 Value
218	Inhibitors
3	kcat/KM [mM/s]
46	Ki Value [mM]
170	KM Value [mM]
48	Localization
5	Metals/Ions
33	Molecular Weight [Da]
145	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
131	Organism
7	Pathway
45	PDB ID
31	pH Optimum
4	pH Range
8	pH Stability
1	pI Value
1	Posttranslational Modification
29	Protein Variants
38	Purification (Commentary)
4	Reaction
4	Reaction Type
127	Reference
126	Source Tissue
25	Specific Activity [micromol/min/mg]
7	Storage Stability
273	Substrates and Products (Substrate)
32	Subunits
124	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
5	Temperature Stability [°C]
41	Turnover Number [1/s]

Application

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Application on EC 1.1.3.15 - (S)-2-hydroxy-acid oxidase

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

agriculture

Solanum lycopersicum

-

inoculation of plants with Pseudomonas syringae increases photorespiration rate and expression of glycolate oxidase (GOX2), serine glyoxylate aminotransferase (SGT) and serine hydroxyl methyltransferase (SHMT1). Silencing of GOX2, SGT or SHMT1 genes in tomato decreases photorespiration but increases susceptibility to Pseudomonas syringae, whereas transient overexpression of GOX2, SGT or SHMT1 in tobacco increases basal defence. Salicylic acid signalling is involved in GOX2-mediated, SGT-mediated and SHMT1-mediated defence. H2O2 pretreatment remarkably alleviates the GOX2 silencing-induced depression in basal defence and salicylic acid signalling

763562

analysis

2 entries

diagnostics

3 entries

drug development

2 entries

medicine

5 entries

molecular biology

Oryza sativa

-

the interaction of capsid protein P8 with the GOX of host cells leads to translocation of capsid protein P8 into peroxisomes. The interaction between capsid protein P8 and GOX plays important roles in Rice dwarf phytoreovirus targeting into the replication site of host cells

673694

synthesis

5 entries

analysis

Pediococcus sp.

-

development of a lactate biosensor through immobilization of lactate oxidase in an albumin and mucin composed hydrogel by gluitaraldehyde cross-linking and trapping between two polycarbonate membranes. Hydrogen peroxide produced is detected on a platinum electrode. The response time of the sensor to 0.010 mM lactate requires 90 s to give a 100% steady-state response of 0.079 microA. Linear behavior is obtained between0.7 microM and 1.5 mM. The detection limit calculated from the signal to noise ratio was 0.7 microM

690071

analysis

Candidatus Caldarchaeum subterraneum

E6N788

construction of a D-Lactate electrochemical biosensor. The electrode for detection of D-lactate is prepared by immobilizing dye-linked D-LDH and multi-walled carbon nanotube within Nafion membrane. The electrode response to D-lactate is linear within the concentration range of 0.03-2.5 mM, and it shows little reduction in responsiveness after 50 days

761556

diagnostics

Pediococcus sp.

-

lactate biosensor (FIA amperometric detection), tested with beer samples, detection limit: 0.84 micromol/l, three-electrode cell with AG/AGCl, NaCl reference electrode, platinum wire, glassy carbon electrode, the latter layered with Prussian Blue film of K3Fe(CN)6, Fe(III), KCl, cetyltrimethylammonium bromide, pH 1.7 maintained with HCl, lactate oxidase immobilized onto the Prussian Blue-modified glassy carbon electrode, enzyme layer covered by a Nafion-ethanol solution, storage in phosphate buffer, pH 6.9

699088

diagnostics

Pediococcus sp.

-

lactate biosensor development based on an enzymatic recognition system using lactate oxidase and a transduction system based on laminol, peroxidase from Arthromyces ramosus and metallic aluminum, immobilized in a plastic support by a polyion complex membrane prepared from poly-L-lysine hydrobromide and poly(sodium 4-styrenesulfonate)

695568

diagnostics

Homo sapiens

Q9NYQ3

the metabolic gene HAO2 is downregulated in hepatocellular carcinoma and predicts metastasis and poor survival. Dysregulation of HAO2 is a very early event in the development of hepatocellular carcinoma and it may represent a useful diagnostic and prognostic marker for human hepatocellular carcinoma

745481

drug development

Homo sapiens

Q9UJM8

in humans the enzyme is a potential drug target for treatment of primary hyperoxaluria, a genetic disorder where overproduction of oxalate results in the formation of kidney stones

698951

drug development

Rattus norvegicus

-

isozyme Hao2 is a target for drug development in high blood pressure

723811

medicine

Aerococcus viridans

-

enzyme is used in clinical chemistry for the determination of (S)-lactate in blood in some pathological diseases such as diabetes, heart diseases and shock syndrome

440391

medicine

Rattus norvegicus

-

decrease of enzyme activity upon oxidative stress induced by glutathione depletion or postischemic perfusion, down-regulation of enzyme as mechanism to prevent excessive H2O2 formation in liver peroxisome

655694

medicine

Mus musculus

Q9WU19

the ability of such siRNAs to reduce urinary oxalate in the mouse model suggests that this approach is promising for the treatment of primary hyperoxalurias, PH, particularly PH type I, in humans, genes HYPDH and GO appear to be the best targets for reducing the production of glyoxylate and oxalate in PH patients

741999

medicine

Mus musculus

Q9WU19

application of oan in vivo CRISPR/Cas9-mediated substrate reduction therapy to treat primary hyperoxaluria type I that results in excessive hepatic oxalate production causing end-stage renal disease. A single systemic administration of an AAV8-CRISPR/Cas9 vector targeting glycolate oxidase, prevents oxalate overproduction and kidney damage, with no signs of toxicity in Agxt1-/- mice

763509

medicine

Mus musculus C57BL/6J

-

the ability of such siRNAs to reduce urinary oxalate in the mouse model suggests that this approach is promising for the treatment of primary hyperoxalurias, PH, particularly PH type I, in humans, genes HYPDH and GO appear to be the best targets for reducing the production of glyoxylate and oxalate in PH patients

-

synthesis

Spinacia oleracea

-

-

389682

synthesis

Spinacia oleracea

-

production of glyoxylate in biocatalysis of co-produced glycolate oxidase and catalase T

389679

synthesis

Spinacia oleracea

-

optimization of enzyme expression in Escherichia coli for production glyoxylate in culture medium

656768

synthesis

Geotrichum candidum

-

optimization of production of lactate oxidase by cultivating strains under high aeration in a medium with 0.5% glucose and 2% lactate for 1 day results in activities of 130-140 U/l

684536

synthesis

Spinacia oleracea

P05414

coexpression of N-demethylase NdmB gene from Pseudomonas putida CBB5 and glycolate oxidase gene in Escherichia coli. By two-step purification of Ni affinity chromatography and Q-Sepharose chromatography, the coexpressed NdmB and GO are separated and result in a 15.8fold purification with 8.7% yield and 12.8fold purification with 7.2% yield, respectively

763461