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1.1.3.13: alcohol oxidase

This is an abbreviated version!
For detailed information about alcohol oxidase, go to the full flat file.

Word Map on EC 1.1.3.13

Reaction

a primary alcohol
+
O2
=
an aldehyde
+
H2O2

Synonyms

alcohol oxidase 1, alcohol oxidase 2, alcohol oxidase A, alcohol oxidase B, alcohol oxidase I, alcohol: O2 oxidoreductase, alcohol:dioxygen-oxidoreductase, alcohol:O2 oxidoreductase, AlcOx, AOd, AOD1, AOext, AOint, AOX, AOX1, AOX2, AOXI, broad substrate specific alcohol oxidase, EC 1.1.3.31, ethanol oxidase, extracellular alcohol oxidase, FAD-dependent alcohol oxidase, FAO1, GLRG_05590, intracellular alcohol oxidase, long chain fatty alcohol oxidase, methanol oxidase, Mod1p, Mod2p, oxidase, alcohol, P-AOD, peroxisomal alcohol oxidase, primary alcohol oxidase, SCAO, short chain alcohol oxidase, short-chain alcohol oxidase, VAO, veratryl alcohol oxidase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.13 alcohol oxidase

Temperature Stability

Temperature Stability on EC 1.1.3.13 - alcohol oxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
-
24 h stable
20 - 60
Pichia putida
-
10% loss of activity at 20°C after 24 h, complete loss of activity after 10 min at 60°C
22
-
50% loss of activity after 10 min in 0.1 M potassium phosphate buffer, pH 6.0. 50% loss of activity after 60 min in 0.1 M potassium phosphate buffer, pH 7.0, 50% loss of activity after 28 h in 0.1 M Tris-HCl buffer, pH 8.0. 50% loss of activity after 16 h in 0.1 M Tris-HCl buffer, pH 9.0
25
-
pH 5.0, 3 h, complete inactivation at pH 5.0 after 3 h
35
-
30 min, loss of 50% activity, inactivation after 100 min
37
-
the enzyme loses its activity after 5 h and 15 h at pH 3.0 and pH 7.0, respectively
40 - 80
-
the immobilized enzyme is fairly stable at temperature up to 60°C. Above 70°C, its activity declines rapidly as the temperature increased, but the immobilized enzyme is not completely inactivated even at 80°C
42
-
purified recombinant His-tagged enzyme, pH 8.0, 60 min, stable
51
melting temperature of mutant F101S, 5 mM phoshate buffer pH 7.5
58
melting temperature of wild-type, 5 mM phoshate buffer pH 7.5
65
-
15 min, complete inactivation above
additional information