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1.1.3.10: pyranose oxidase

This is an abbreviated version!
For detailed information about pyranose oxidase, go to the full flat file.

Word Map on EC 1.1.3.10

Reaction

D-glucose
+
O2
=
2-dehydro-D-glucose
+
H2O2

Synonyms

C-2 specific pyranose-2-oxidase, carbohydrate oxidase, glucose 2-oxidase, glucose-2-oxidase, P2O, P2Ox, POX, PROD, PyOx, pyranose 2-Oxidase, pyranose oxidase, pyranose-2-oxidase, pyranose/oxygen 2-oxidoreductase, pyranose: oxygen 2-oxidoreductase, pyranose:oxygen 2-oxidoreductase, pyranose:oxygen-2-oxidoreductase, TmP2Ox

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.10 pyranose oxidase

Purification

Purification on EC 1.1.3.10 - pyranose oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, phenyl Sepharose 6 column chromatography, DEAE-Sepharose column chromatography, and Sephacryl S-300 gel filtration
-
anion exchange chromatography and preparative native PAGE
continuous homogenizer, IMAC Ni-Charged Resin
epoxy-activated Sepharose 6B-IDA-Cu(II) column column chromatography
-
HisTrap column chromatography
-
homogenized in a French press, one-step immobilized metal affinity chromatography, concentrated in 50mM potassium phosphate buffer
IMAC Ni-charged resin column chromatography
immobilized metal ion affinity chromatography
Ni-NTA agarose resin column chromatography and Superdex 200 gel filtration
Ni2+ immobilized column with Chelating Sepharose Fast Flow combined with Äkta Purifier System, concentrated in an Amicon Ultra Centrifugal Filter Device
Ni2+-immobilized metal affinity column chromatography
partial purification, PEG 4000 precipitation, DEAE-Sepharose column chromatography, and gel filtration
Polyporus obtusus
-
protein purification by immobilized metal affinity chromatography, ultrafiltration, concentration of enzymes
-
recmbinant enzyme from Escherichia coli by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
-
recombinant His-tagged enzyme 232fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme 6.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinit chromatography and ulrafiltration
recombinant protein, gel purification and SDS-PAGE
recombinant protein, gel purified to specific activity of 4.2 U/mg
-
recombinant wild-type and mutant enzymes
stored, wet cell paste is treated with lysozyme, PMSF, EDTA buffer. Following steps are RNAse and DNAse treatment, sonication, dialyse, DEAE Sepharose Fast Flow column, dialyse, Phenyl Sepharose Fast Flow column, concentration by ammonium sulfate saturation, final dialyse. Stored at 20°C
-
using ammonium sulfate fractionation, hydrophobic interaction, ion-exchange chromatography, and gel filtration
-
using ammonium sulfate treatment, column chromatography on phenyl-Sepharose CL-4B, DEAE-Sephacel and chromatofocusing
-
using column chromatography on HPA-75, Phenyl-Sepharose CL-6B and cellulofine GCL-2000 superfine
-
using column chromatography on HPA-75, Sepharose 4B, Sephadex G-100 gel filtration and hydroxylapatite chromatography
-
using fractional precipitation with polyethylene glycol
Polyporus obtusus
-
using fractional precipitation with polyethylene glycol and acid treatment
Polyporus obtusus
-
using fractionated ammonium sulfate precipitation, chromatography on DEAE-Sephacel, Sephacryl S 300, S Sepharose and Q Sepharose
-
using heat treatment of the mycelium extract and immunoaffinity chromatography
Phanerochaete gigantea
-
using heat treatment, immunoaffinity chromatography and gel filtration on Superdex 200
-
using hydrophobic interaction chromatography on phenyl-Sepharose CL-4B, anion-exchange chromatography on a Mono-Q column and chromatofocusing on a Mono-P column
-
using liquid chromatography on phenyl Sepharose, Mono Q twice and phenyl Superose
-
wild-type and recombinant protein, gel purification