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1.1.3.10: pyranose oxidase

This is an abbreviated version!
For detailed information about pyranose oxidase, go to the full flat file.

Word Map on EC 1.1.3.10

Reaction

D-glucose
+
O2
=
2-dehydro-D-glucose
+
H2O2

Synonyms

C-2 specific pyranose-2-oxidase, carbohydrate oxidase, glucose 2-oxidase, glucose-2-oxidase, P2O, P2Ox, POX, PROD, PyOx, pyranose 2-Oxidase, pyranose oxidase, pyranose-2-oxidase, pyranose/oxygen 2-oxidoreductase, pyranose: oxygen 2-oxidoreductase, pyranose:oxygen 2-oxidoreductase, pyranose:oxygen-2-oxidoreductase, TmP2Ox

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.10 pyranose oxidase

Crystallization

Crystallization on EC 1.1.3.10 - pyranose oxidase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystallized in hanging drops containing 0.002 ml of a 7 mg/ml protein solution and 0.002 ml of reservoir buffer, 19% PEG-6000 in 100 mM MES, pH 6.0, at 20°C
hanging drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.5), 0.1 M MnCl2, and 30% (w/v) polyethylene glycol 400
hanging drop method
-
hanging drop vapor diffusion method, using 0.1 M MES (pH 5.2), 50 mM MgCl2, and 10% (w/v) monomethylether polyethylene glycol 2000
mutant enzymes are crystallized by the hanging drop vapor diffusion method, using 10% (w/v) monomethyl ether poly(ethlene glycol) 2000 , 0.1 M MES (pH 5.2), 50 mM MgCl2
mutant enzymes H167A and T169, Ahanging drop vapor diffusion method, using 0.1 M sodium acetate (pH 4.6), 50 mM MgCl2, and 6% (w/v) monomethyl ether PEG 2000
P2O H167A mutant in complex with 3-deoxy-3-fluoro-D-glucose, microseeded hanging drops, mixing of equal volumes of 0.1 M MES, pH 5.2, 50 mM MgCl2, 10% w/v PEG monomethyl ether 2000 with 20 mg/ml protein in 20 mM MES, pH 5.2, addition of ligand in saturated mother liquor with 28% w/v PEG 2000, X-ray diffraction structure determination and analysis at 1.35 A resolution
wild-type and mutants, structure analysis, overview
-