Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.1.2.8: alcohol dehydrogenase (cytochrome c)

This is an abbreviated version!
For detailed information about alcohol dehydrogenase (cytochrome c), go to the full flat file.

Word Map on EC 1.1.2.8

Reaction

a primary alcohol
+
2 ferricytochrome c
=
an aldehyde
+ 2 ferrocytochrome c + 2 H+

Synonyms

Ca2+-dependent PQQ-ADH, EC 1.1.99.8, EDH, ethanol dehydrogenase, exaA, exaF, PedE, PedH, PpADH, PP_2674, PP_2679, PQQ-ADH, PQQ-alcohol dehydrogenase, PQQ-dependent alcohol dehydrogenase, PQQ-dependent type I alcohol dehydrogenase, PQQ-DH9, pyrroloquinoline quinone ethanol dehydrogenase, pyrroloquinoline quinone-dependent alcohol dehydrogenases, pyrroloquinoline quinone-dependent dehydrogenase, pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase, pyrroquinoline quinone-dependent alcohol dehydrogenase, quinoprotein alcohol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.2 With a cytochrome as acceptor
                1.1.2.8 alcohol dehydrogenase (cytochrome c)

Purification

Purification on EC 1.1.2.8 - alcohol dehydrogenase (cytochrome c)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
C-terminally His-tagged PQQ-ADHs are expressed in Escherichia coli BL21(DE3)
native enzyme from membranes by two steps of anion exchange chormatography, hydroxyapatite chromatography, and gel filtration
-
native enzyme from strain IFO3191 by ultracentrifugation and anion exchange chromatography
native enzyme from strain MSU10 by ultracentrifugation and anion exchange chromatography
native enzyme from strain SKU1108 by ultracentrifugation and anion exchange chromatography
recombinant His6-tagged enzyme from Escherichia coli strain TOP10 by ultracentrifugation, nickel affinity chromatography, ultrafiltration, and desalting gel filtration
-