1.1.1.B4: (R)-specific secondary alcohol dehydrogenase (NADH)
This is an abbreviated version!
For detailed information about (R)-specific secondary alcohol dehydrogenase (NADH), go to the full flat file.
Reaction
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=
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Synonyms
(R)-1-phenylethanolsynthase, (R)-epinephrine dehydrogenase, ADH, ADH1, ADH2, ADHTt, carbonyl reductase, carbonyl reductase (NADH, specific for (S)-configuration of alcohol), CHY1186, CpCR, mLDH, More, RPES, SADH, SDR, secondary alcohol dehydrogenase, short chain dehydrogenase, short-chain NAD(H)-dependent alcohol dehydrogenase
ECTree
Temperature Stability
Temperature Stability on EC 1.1.1.B4 - (R)-specific secondary alcohol dehydrogenase (NADH)
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20 - 40
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purified enzyme, stable at
30
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wild-type, half-life 160 h, mutant R38P, half-life 220 h
42
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wild-type, half-life 1.25 h, mutant R38P, half-life 3 h
50
-
24 h, purified recombinant enzyme, 142% inactivation
55
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purified enzyme, retains almost 40% of maximal activity for 60 min
90
-
30 min, purified recombinant enzyme, 50% inactivation
60
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purified enzyme, 60 min, inactivation
60
-
24 h, purified recombinant enzyme, 134% inactivation
70
-
60 min, pH 7.0, 60% residual activity
70
-
24 h, purified recombinant enzyme, 107% inactivation