1.1.1.B4: (R)-specific secondary alcohol dehydrogenase (NADH)
This is an abbreviated version!
For detailed information about (R)-specific secondary alcohol dehydrogenase (NADH), go to the full flat file.
Reaction
Synonyms
(R)-1-phenylethanolsynthase, (R)-epinephrine dehydrogenase, ADH, ADH1, ADH2, ADHTt, carbonyl reductase, carbonyl reductase (NADH, specific for (S)-configuration of alcohol), CHY1186, CpCR, mLDH, More, RPES, SADH, SDR, secondary alcohol dehydrogenase, short chain dehydrogenase, short-chain NAD(H)-dependent alcohol dehydrogenase
ECTree
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KM Value
KM Value on EC 1.1.1.B4 - (R)-specific secondary alcohol dehydrogenase (NADH)
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11.2
2,2,2-trifluoroacetophenone
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pH 6.0, 65°C, recombinant enzyme, reduction reaction
1
ethylbenzoylformate
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pH 6.0, 65°C, recombinant enzyme, reduction reaction
0.41
(R)-2-butanol
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wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
37.4
(R)-2-butanol
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mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.22
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wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
11.7
(R)-2-pentanol
-
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.32
3,5-bis(trifluoromethyl)acetophenone
mutant S154Y/L194I, pH 7.2, 30°C
additional information
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Michaelis-Menten kinetics
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additional information
additional information
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Michaelis-Menten kinetics
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additional information
additional information
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kinetics and substrate specificity, overview
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