1.1.1.B4: (R)-specific secondary alcohol dehydrogenase (NADH)

This is an abbreviated version!
For detailed information about (R)-specific secondary alcohol dehydrogenase (NADH), go to the full flat file.

Reaction

Synonyms

(R)-1-phenylethanolsynthase, (R)-epinephrine dehydrogenase, ADH, ADH1, ADH2, ADHTt, carbonyl reductase, carbonyl reductase (NADH, specific for (S)-configuration of alcohol), CHY1186, CpCR, mLDH, More, RPES, SADH, SDR, secondary alcohol dehydrogenase, short chain dehydrogenase, short-chain NAD(H)-dependent alcohol dehydrogenase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.B4 (R)-specific secondary alcohol dehydrogenase (NADH)

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Results	in table
1	Activating Compound
5	AA Sequence
11	Application
7	Cloned(Commentary)
27	Cofactor
3	Crystallization (Commentary)
1	General Information
34	Inhibitors
38	kcat/KM [mM/s]
46	KM Value [mM]
3	Localization
16	Metals/Ions
11	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
10	Organic Solvent Stability
30	Organism
2	Pathway
16	pH Optimum
3	pH Range
1	pH Stability
1	pI Value
14	Protein Variants
8	Purification (Commentary)
3	Reaction
27	Reference
2	Source Tissue
4	Specific Activity [micromol/min/mg]
1	Storage Stability
154	Substrates and Products (Substrate)
11	Subunits
31	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
2	Temperature Range [°C]
10	Temperature Stability [°C]
41	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 1.1.1.B4 - (R)-specific secondary alcohol dehydrogenase (NADH)

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.26 - 1.6

1-(2-pyridyl)acetone

2 entries

0.26 - 0.51

1-(2-thienyl)acetone

3 entries

0.2 - 0.63

1-(3-pyridyl)acetone

2 entries

4.3

1-(3-thienyl)acetone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, mutant I86A

741784

1.05 - 2.4

1-(4-pyridyl)acetone

2 entries

1.5 - 8.1

1-phenylpropanone

2 entries

0.23 - 0.62

2',4'-difluoro-acetophenone

2 entries

0.018

2'-methylacetophenone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

50.1

2,3-Butanedione

Gluconobacter oxydans

-

pH 7.4, 30°C

740394

0.65

3'-bromo-acetophenone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

0.41 - 1.3

3'-chloro-acetophenone

2 entries

0.16

3'-iodo-acetophenone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

0.21

3'-methoxy-acetophenone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

0.18

3'-methylacetophenone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

0.025

3'-trifluoromethyl-acetophenone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

36.8 - 463

3,5-bis(trifluoromethyl)acetophenone

3 entries

1.08 - 1.33

acetone

2 entries

1.9 - 14.9

acetophenone

2 entries

4.1

ethyl 4-chloro-3-oxobutanoate

Gluconobacter oxydans

-

pH 7.4, 30°C

740394

75 - 4207

NADH

5 entries

5100 - 9300

NADPH

2 entries

0.26

1-(2-pyridyl)acetone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

1.6

1-(2-pyridyl)acetone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, mutant I86A

741784

0.26

1-(2-thienyl)acetone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

0.35

1-(2-thienyl)acetone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

0.51

1-(2-thienyl)acetone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, mutant I86A

741784

0.2

1-(3-pyridyl)acetone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, mutant I86A

741784

0.63

1-(3-pyridyl)acetone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

1.05

1-(4-pyridyl)acetone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, mutant I86A

741784

2.4

1-(4-pyridyl)acetone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

1.5

1-phenylpropanone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

8.1

1-phenylpropanone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, mutant I86A

741784

0.23

2',4'-difluoro-acetophenone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

0.62

2',4'-difluoro-acetophenone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, mutant I86A

741784

0.41

3'-chloro-acetophenone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, mutant I86A

741784

1.3

3'-chloro-acetophenone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

36.8

3,5-bis(trifluoromethyl)acetophenone

Leifsonia xyli

T2FLN4

wild-type, pH 7.2, 30°C

739942

277

3,5-bis(trifluoromethyl)acetophenone

Leifsonia xyli

T2FLN4

mutant S154Y/L194I, pH 7.2, 30°C

739942

463

3,5-bis(trifluoromethyl)acetophenone

Leifsonia xyli

T2FLN4

mutant S154Y, pH 7.2, 30°C

739942

1.08

acetone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

1.33

acetone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, mutant I86A

741784

1.9

acetophenone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, wild-type enzyme

741784

14.9

acetophenone

Thermoanaerobacter ethanolicus

-

pH 6.5, 50°C, mutant I86A

741784

75

NADH

Levilactobacillus brevis

-

wild-type, pH 6.5, 30°C

711860

300

NADH

Levilactobacillus brevis

-

mutant R38P, pH 5.5, 30°C

711860

944

NADH

Leifsonia xyli

T2FLN4

wild-type, pH 7.2, 30°C

739942

3846

NADH

Leifsonia xyli

T2FLN4

mutant S154Y/L194I, pH 7.2, 30°C

739942

4207

NADH

Leifsonia xyli

T2FLN4

mutant S154Y, pH 7.2, 30°C

739942

5100

NADPH

Levilactobacillus brevis

-

mutant R38P, pH 5.5, 30°C

711860

9300

NADPH

Levilactobacillus brevis

-

wild-type, pH 6.5, 30°C

711860