1.1.1.87: homoisocitrate dehydrogenase

This is an abbreviated version!
For detailed information about homoisocitrate dehydrogenase, go to the full flat file.

Reaction

Synonyms

(-)-1-hydroxy-1,2,4-butanetricarboxylate:NAD+ oxidoreductase (decarboxylating), 2-hydroxy-3-carboxyadipate dehydrogenase, 3-carboxy-2-hydroxyadipate dehydrogenase, 3-carboxy-2-hydroxyadipate:NAD+ oxidoreductase (decarboxylating), beta-decarboxylating dehydrogenase, dehydrogenase, homoisocitrate, EC 1.1.1.155, HIc, HIc dehydrogenase, HICDH, homoisocitrate dehydrogenase, homoisocitric dehydrogenase, isocitrate-homoisocitrate dehydrogenase, LYS12, protein PH1722, ScHICDH, TK0280

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.87 homoisocitrate dehydrogenase

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Results	in table
2	Activating Compound
335	AA Sequence
5	Application
2	CAS Registry Number
9	Cloned(Commentary)
21	Cofactor
3	Crystallization (Commentary)
8	General Information
2	General Stability
3	IC50 Value
32	Inhibitors
40	kcat/KM [mM/s]
15	Ki Value [mM]
93	KM Value [mM]
30	Metals/Ions
8	Molecular Weight [Da]
22	Natural Substrates/ Products (Substrates)
58	Organism
9	Pathway
14	PDB ID
17	pH Optimum
6	pH Range
1	pH Stability
23	Protein Variants
11	Purification (Commentary)
3	Reaction
4	Reaction Type
30	Reference
9	Source Tissue
13	Specific Activity [micromol/min/mg]
4	Storage Stability
93	Substrates and Products (Substrate)
10	Subunits
41	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
2	Temperature Stability [°C]
63	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.1.1.87 - homoisocitrate dehydrogenase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

His6-tagged recombinant apoenzyme, hanging drop vapor diffusion method, using 100 mM HEPES-NaOH (pH 7.5), 200 mM NaCl, and 10% (v/v) 2-propanol. Enzyme in complex with homoisocitrate and Mn2+, hanging drop vapor diffusion method, using 100 mM HEPES-NaOH (pH 7.5), 200 mM NaCl, and 10% (v/v) 2-propanol. Enzyme in complex with isocitrate and Mn2+, hanging drop vapor diffusion method, using 100 mM Tris-HCl (pH 7.5), 200 mM NaCl, and 30% (v/v) 2-methyl-2,4-pentanediol. Enzyme in complex with 3-isopropylmalate and Mn2+, hanging drop vapor diffusion method, using 100 mM HEPES-NaOH (pH 7.5), 200 mM NaCl, and 36% (v/v) 2-methyl-2,4-pentanediol

Thermococcus kodakarensis

-

760522

enzyme in binary complex with inhibitor (2S,3S)-thiahomoisocitrate, sitting drop vapour diffusion method, 0.00 ml of 8.7 mg/ml protein in 5 mM Tris-HCl, pH 7.8. 0.8 mM inhibitor, and 1.7 mM NAD, is mixed with 0.002 ml of reservoir solution containing 40% 2-methyl-2,4-pentandiol and 100 mM citrate pH 4.85, eqilibration against 1 ml resetvoir solution, X-ray diffrcation structure determination and analysis at 2.6 A resolution, molecular replacement

Thermus thermophilus

Q5SIJ1

722569

purified recombinant enzyme, hanging drop vapour diffusion method, 10 mg/ml protein with reservoir solution containing 24% PEG 400, and 0.1 M citrate, pH 4.8, addition of 0.003 ml drops of 0.1 M MgCl2, 5 mM isocitrate or homoisocitrate, and of a 0.001 ml drop 50 mM CdCl2, equilibration against 0.5 ml reservoir solution, 5 days at 20°C, X-ray diffraction structure determination and analysis at 1.85 A resolution, molecular replacement

Thermus thermophilus

-

669109