1.1.1.86: ketol-acid reductoisomerase (NADP+)

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For detailed information about ketol-acid reductoisomerase (NADP+), go to the full flat file.

Word Map on EC 1.1.1.86

1.1.1.86

branched-chain

5-phosphate

karis

1-deoxy-d-xylulose

fosmidomycin

isoprenoids

methylerythritol

1-deoxy-d-xylulose-5-phosphate

deoxyxylulose

l-valine

isobutanol

2-c-methyl-d-erythritol

non-mevalonate

dihydroxyacid

2-keto

isoleucine-valine

ilvbnce

drug development

biofuel production

industry

Reaction

Synonyms

2-hydroxy-3-keto acid reductoisomerase, acetohydroxy acid isomeroreductase, acetohydroxy acid reductoisomerase, acetohydroxy-acid isomeroreductase , acetohydroxy-acid reductoisomerase , acetohydroxyacid isomeroreductase, acetolactate reductoisomerase, AHAIR, AHIR, alpha-keto-beta-hydroxylacil reductoisomerase, alpha-keto-beta-hydroxylacyl reductoisomerase, class II ketol-acid reductoisomerase, dehydrogenase, dihydroxyisovalerate (isomerizing), dihydroxyisovalerate dehydrogenase (isomerizing), EC 1.1.1.89, Icl1p, Ilv5p, ilvC, IlvC-PanE, IlvC1, IlvC2, isomerase, ketol acid reducto-, isomeroreductase, KARI, ketol-acid reductoisomerase, reductoisomerase

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.86 ketol-acid reductoisomerase (NADP+)

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Results	in table
1	Activating Compound
32999	AA Sequence
7	Application
1	CAS Registry Number
24	Cloned(Commentary)
58	Cofactor
24	Crystallization (Commentary)
37	General Information
2	General Stability
222	Inhibitors
137	kcat/KM [mM/s]
77	Ki Value [mM]
225	KM Value [mM]
5	Localization
62	Metals/Ions
28	Molecular Weight [Da]
72	Natural Substrates/ Products (Substrates)
125	Organism
12	Pathway
139	PDB ID
20	pH Optimum
7	pH Range
8	pH Stability
1	pI Value
1	Posttranslational Modification
52	Protein Variants
29	Purification (Commentary)
5	Reaction
4	Reaction Type
107	Reference
1	Renatured (Commentary)
10	Source Tissue
17	Specific Activity [micromol/min/mg]
3	Storage Stability
278	Substrates and Products (Substrate)
29	Subunits
123	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
2	Temperature Range [°C]
7	Temperature Stability [°C]
162	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 1.1.1.86 - ketol-acid reductoisomerase (NADP+)

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110000

Spinacia oleracea

dimeric wild-type enzyme, gel filtration

639185

115000

2 entries

120000

Spinacia oleracea

2 * 120000, Arabidopsis thaliana acetohydroxy acid synthase genetically fused in frame with the nucleotide sequence coding for the Spinacia oleracea acetohydroxy acid isomeroreductase and expressed in Escherichia coli, SDS-PAGE

205000

220000

228000

sedimentation equilibrium analysis

230000

gel filtration

235000

SDS-PAGE, isoenzyme 1

639176

240000

Spinacia oleracea

Arabidopsis thaliana acetohydroxy acid synthase genetically fused in frame with the nucleotide sequence coding for the Spinacia oleracea acetohydroxy acid isomeroreductase and expressed in E. coli, gel filtration

639184

266000

Pseudomonas aeruginosa

native molecular mass

654058

36100

Corynebacterium glutamicum

SDS-PAGE

39000

40000

1 * 40000, SDS-PAGE

440000

Saccharolobus solfataricus

763188

52428

Escherichia coli

P05793

4 * 52428, calculated

55000

x * 55000, SDS-PAGE

56850

2 * 56850, calculation from acid sequence

57000

59000

60000

monomeric mutant enzyme DELTA423-431/F431S, gel filtration

639185

70000

Sulfolobus acidocaldarius

Q4J8K9

gel filtration

763730

72000

Mycobacterium tuberculosis

P9WKJ7

multi-angle light scattering

738219