Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ca2+
-
0.5 mM, very minor stimulatory effect
Fe2+
-
0.5 mM, very minor stimulatory effect
Rb+
-
RbCl enhances activity
Zn2+
-
0.5 mM, very minor stimulatory effect
Cd2+
-
divalent cation required, Mn2+ and Cd2+ are about equally effective at 0.5 mM
Cd2+
-
divalent cation required
Cd2+
-
divalent cation required, 93% of the activity with Mn2+
Cd2+
-
required for activity, 0.2 M
Cd2+
-
0.5 mM, slight activation
Co2+
-
divalent cations required 0.5 mM, Co2+ is 73% as effective as Mn2+ or Co2+
Co2+
-
divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+
Co2+
-
divalent cation required
Co2+
-
divalent cation required, 43% of the activity with Mn2+
Co2+
-
required for activity, 0.2 M
K+
-
monovalent cation required
K+
-
0.3 mM required for optimal activity
K+
-
monovalent cation required, maximal activity at 0.05 M
K+
-
1.5fold stimulation, optimal concentration: 0.2 M
K+
-
slight stimulation, but not essential. Optimal concentration 0.2 M
K+
-
about 1.5fold stimulation, optimal concentration around 0.2 M
K+
-
in 1.1 M potassium phosphate buffer, pH 7.6, the activity is over 20times that in 0.1 M sodium phosphate. Optimal KCl concentration is above 2 M, enhances activity 20times
Mg2+
-
1.0 mM, enhances activity 4.7fold
Mg2+
-
divalent cation required, saturation is reached at 0.2 mM, activity with Mn2+ is twice as high as with Mg2+
Mg2+
-
divalent cations required 0.5 mM Mg2+ is 62% as effective as Mn2+ or Co2+
Mg2+
-
divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+
Mg2+
-
divalent cation required
Mg2+
-
divalent cation required, 76% of the activity with Mn2+
Mg2+
-
required for activity, 0.2 M
Mg2+
-
divalent cation required, most active in presence of 0.1 mM Mn2+ or 1 mM Mg2+
Mn2+
-
1.0 mM, enhances activity 4.2fold
Mn2+
-
divalent cation required, saturation is reached at 0.2 mM, activity with Mn2+ is twice as high as with Mg2+
Mn2+
-
divalent cation required, Mn2+ and Cd2+ are about equally effective at 0.5 mM
Mn2+
-
divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+
Mn2+
-
divalent cation required
Mn2+
-
divalent cation required, maximum activity in presence of Mn2+
Mn2+
-
required for activity, 0.2 M
Mn2+
-
divalent cation required, most active in presence of 0.1 mM Mn2+ or 1 mM Mg2+
Mn2+
-
required. Only the metal-threo-D-3-isopropylmalate complex, but neither the metal-ion nor the free threo-D-3-isopropylmalate itself, is efficient in stabilizing the native protein conformation. In the absence of metal ion, only a very marginal extent of domain closure takes place
NH4+
-
monovalent cation required
NH4+
-
monovalent cations required, NH4+ can partially replace K+
NH4+
-
NH4Cl, optimal concentration is about 0.4 M, enhances activity 18times