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1.1.1.76: (S,S)-butanediol dehydrogenase

This is an abbreviated version!
For detailed information about (S,S)-butanediol dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.76

Reaction

(2S,3S)-butane-2,3-diol
+
NAD+
=
(S)-acetoin
+
NADH
+
H+

Synonyms

(2R,3R)-2,3-BDH, (2R,3R)-2,3-butanediol dehydrogenase, (2S, 3S)-BDH, (2S, 3S)-butanediol dehydrogenase, (S,S)-2,3-BDH, (S,S)-2,3-butanediol dehydrogenase, (S,S)-butanediol dehydrogenase, 2,3-BDH, 2,3-butanediol dehydrogenase, acetoin(diacetyl) reductase, AdR, ADS1, ARA1, BDH, budC, ButA, L(+)-2,3-butanediol dehydrogenase (L-acetoin forming), L-(2S,3S)-butanediol dehydrogenase, L-BDH, L-butanediol dehydrogenase, LBDH, mbdh, meso-2,3-butanediol dehydrogenase, More, S-2,3-butanediol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.76 (S,S)-butanediol dehydrogenase

Cloned

Cloned on EC 1.1.1.76 - (S,S)-butanediol dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-expressed with glucose dehydrogenase and formate dehydrogenase in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
-
expression in Escherichia coli JM109, stereospecificity of resulting L-butanediol dehydrogenase is reduced. Expressed gene contains tac-promoter and upstream region of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae IAM1063. A more stable stereospecificity is exhibited
-
expression in Escherichia coli JM109/pLBD2-CTC
-
gene budC, recombinant expression of SUMO-His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-Gold from plasmid pET28a-SUMO-KpBDH
gene mbdh, recombinant expression of the enzyme in Escherichia coli strain BL21(DE3)
-
genetic structure of the 2,3-BD utilization operon in Pseudomonas aeruginosa strain PAO1 and comparisons, overview
-
L-butanediol gene containing plasmid pLBD2-CTC is expressed in Escherichia coli JM109
-
recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
When the enzyme gene is only expressed in Escherichia coli using the tac promoter, the stereospecificity of resulting enzyme gene is reduced. When the region upstream of the meso-butanediol dehydrogenase gene of Klebsiella pneumoniae is also involved in the expression of gene, the resulting enzyme exhibits more stable stereospecificity.
-