1.1.1.67: mannitol 2-dehydrogenase
This is an abbreviated version!
For detailed information about mannitol 2-dehydrogenase, go to the full flat file.
Word Map on EC 1.1.1.67
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1.1.1.67
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polyol
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fluorescens
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d-fructose
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synthesis
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5-dehydrogenase
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1.1.1.138
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heterofermentative
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reuteri
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gluconobacter
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industry
-
pfldh
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arabitol
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molasses
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nutrition
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biotechnology
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analysis
- 1.1.1.67
-
polyol
- fluorescens
- d-fructose
- synthesis
-
5-dehydrogenase
-
1.1.1.138
-
heterofermentative
- reuteri
- gluconobacter
- industry
- pfldh
- arabitol
- molasses
- nutrition
- biotechnology
- analysis
Reaction
Synonyms
alcohol dehydrogenase, zinc-containing, D-mannitol dehydrogenase, M2DH, mannitol 2-dehydrogenase, mannitol dehydrogenase, mannitol-2-dehydrogenase, MDH, mt-dh, MtDH, MtlD, NAD+-dependent mannitol dehydrogenase, NADH-dependent mannitol dehydrogenase, pfMDH, polyol dehydrogenase, PsM2DH, TM0298, TM_0298
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Substrates Products
Substrates Products on EC 1.1.1.67 - mannitol 2-dehydrogenase
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REACTION DIAGRAM
D-fructose + polyethylenimine-NH-succinyl-NADH + H+
D-mannitol + polyethylenimine-NH-succinyl-NAD+
-
-
-
-
r
D-glucitol + NAD+
?
4% of the activity with D-mannitol
-
-
?
D-mannitol + polyethyleneglycol-NH-succinyl-aminoethyl-NAD+
D-fructose + polyethyleneglycol-NH-succinyl-aminoethyl-NADH
-
-
-
?
D-mannitol + polyethyleneglycol-NH-succinyl-NAD+
D-fructose + polyethyleneglycol-NH-succinyl-NADH
-
-
-
?
D-mannitol + polyethylenimin-NH-succinyl-NAD+
D-fructose + polyethylenimin-NH-succinyl-NADH
-
-
-
?
D-tagatose + NAD(P)+
? + NAD(P)H
29% relative activity compared to D-fructose
-
-
?
D-tagatose + NAD+
?
29% relative activity on D-tagatose compared to 100% activity on D-fructose
-
-
?
D-xylose + NADH + H+
D-xylitol + NAD+
18% activity compared to D-fructose
-
-
r
D-xylulose + NAD(P)+
? + NAD(P)H
18% relative activity compared to D-fructose
-
-
?
D-xylulose + NAD+
?
18% relative activity on D-xylulose compared to 100% activity on D-fructose
-
-
?
L-sorbose + NAD(P)+
? + NAD(P)H
5% relative activity compared to D-fructose
-
-
?
targatose + NADH + H+
? + NAD+
29% activity compared to D-fructose
-
-
r
D-fructose + NADH + H+
D-mannitol + NAD+
-
-
-
r
D-fructose + NADH + H+
D-mannitol + NAD+
-mannitol production
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-
r
D-fructose + NADH + H+
D-mannitol + NAD+
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-
-
r
D-fructose + NADH + H+
D-mannitol + NAD+
-mannitol production
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-
r
D-fructose + NADH + H+
D-mannitol + NAD+
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Asn300 has an auxiliary role in stabilization of the transition state of hydride transfer and His303 contributes to substrate positioning, role of Lys295 in general base enzymic catalysis
-
-
?
D-fructose + NADH + H+
D-mannitol + NAD+
the Thermotoga maritima MtDH pathway produces D-mannitol from glucose in two steps: first the xylose isomerase from Thermotoga neapolitana converts glucose to fructose, then MtDH converts D-fructose to D-mannitol
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r
D-fructose + NADH + H+
D-mannitol + NAD+
the Thermotoga maritima MtDH pathway produces D-mannitol from glucose in two steps: first the xylose isomerase from Thermotoga neapolitana converts glucose to fructose, then MtDH converts D-fructose to D-mannitol
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r
D-fructose + NADH + H+
D-mannitol + NAD+
the Thermotoga maritima MtDH pathway produces D-mannitol from glucose in two steps: first the xylose isomerase from Thermotoga neapolitana converts glucose to fructose, then MtDH converts D-fructose to D-mannitol
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r
D-fructose + NADH + H+
D-mannitol + NAD+
the Thermotoga maritima MtDH pathway produces D-mannitol from glucose in two steps: first the xylose isomerase from Thermotoga neapolitana converts glucose to fructose, then MtDH converts D-fructose to D-mannitol
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-
r
D-fructose + NADH + H+
D-mannitol + NAD+
the Thermotoga maritima MtDH pathway produces D-mannitol from glucose in two steps: first the xylose isomerase from Thermotoga neapolitana converts glucose to fructose, then MtDH converts D-fructose to D-mannitol
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-
r
D-fructose + NADPH + H+
D-mannitol + NADP+
also active on fructose with NADPH
-
-
?
D-fructose + NADPH + H+
D-mannitol + NADP+
low activity
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-
r
D-fructose + NADPH + H+
D-mannitol + NADP+
low activity
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-
r
D-fructose + NADPH + H+
D-mannitol + NADP+
low activity
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-
r
D-fructose + NADPH + H+
D-mannitol + NADP+
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
also active on fructose with NADPH
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-
?
D-fructose + NADPH + H+
D-mannitol + NADP+
low activity
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-
r
D-mannitol + NAD(P)+
D-fructose + NAD(P)H + H+
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
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-
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r
D-mannitol + NAD+
D-fructose + NADH + H+
free energy profiles for the enzymatic reaction suggest that enzyme primarily acts in D-mannitol oxidation
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r
D-mannitol + NAD+
D-fructose + NADH + H+
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enzyme highly specific for D-mannitol and D-fructose
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r
D-mannitol + NAD+
D-fructose + NADH + H+
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enzyme highly specific for D-mannitol and D-fructose
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r
D-mannitol + NAD+
D-fructose + NADH + H+
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the epsilon-NH2 group of Lys295 participates in an obligatory pH-dependent, pre-catalytic equilibrium which may control alcohol/alkoxide equilibration of the enzyme-bound D-mannitol and activates the C2 atom for subsequent catalytic oxidation by NAD+
-
-
?
D-mannitol + NAD+
D-fructose + NADH + H+
wild-type enzyme
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-
r
D-mannitol + NAD+
D-fructose + NADH + H+
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
100% activity
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-
r
D-fructose + NADPH + H+
M2DH is a much poorer enzyme when it employes NADP+ and NADPH as compared to NAD+ and NADH
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-
r
D-mannitol + NADP+
D-fructose + NADPH + H+
32% of the activity with NAD+
-
-
?
L-sorbose + NADH + H+
L-sorbitol + NAD+
5% relative activity on L-sorbose compared to 100% activity on D-fructose
-
-
?
L-sorbose + NADH + H+
L-sorbitol + NAD+
5% activity compared to D-fructose
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-
r
L-sorbose + NADH + H+
L-sorbitol + NAD+
5% activity compared to D-fructose
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-
r
L-sorbose + NADH + H+
L-sorbitol + NAD+
5% activity compared to D-fructose
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r
L-sorbose + NADH + H+
L-sorbitol + NAD+
5% activity compared to D-fructose
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-
r
L-sorbose + NADH + H+
L-sorbitol + NAD+
5% activity compared to D-fructose
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r
?
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a D-arabo configuration is required for a polyol substrate to become reactive. The C2 (R) configuration as in mannitol is preferred over the C2 (S) configuration as in D-sorbitol
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?
additional information
?
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slightly active with sorbitol, no activity with ribitol, arabinitol, or mesoerythritol
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?
additional information
?
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among the Escherichia coli strains, BL21 (DE3) plysS exhibits the maximum expression level of MDH (11mg/L)
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-
?
additional information
?
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no activity with glycerol, 1, 2-hexanediol, and 1,2,3-hexanetriol
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-
?
additional information
?
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no activity with glycerol, 1, 2-hexanediol, and 1,2,3-hexanetriol
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-
?
additional information
?
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no activity with NADP+ and NADPH
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-
?
additional information
?
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mannitol can be produced directly from glucose in a two-step enzymatic process, using a Thermotoga neapolitana xylose isomerase mutant and TmMtDH at 60°C. No activity with glucose, xylose, threonine, arabinose, acetaldehyde, 2-butanone, sorbitol, xylitol, ethanol, or 2-butanol
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-
?
additional information
?
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mannitol can be produced directly from glucose in a two-step enzymatic process, using a Thermotoga neapolitana xylose isomerase mutant and TmMtDH at 60°C. No activity with glucose, xylose, threonine, arabinose, acetaldehyde, 2-butanone, sorbitol, xylitol, ethanol, or 2-butanol
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-
?
additional information
?
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TM0298 shows no detectable activity on glucose, xylose, threonine, arabinose, acetaldehyde, 2-butanone, sorbitol, xylitol, ethanol, or 2-butanol
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-
?
additional information
?
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TM0298 shows no detectable activity on glucose, xylose, threonine, arabinose, acetaldehyde, 2-butanone, sorbitol, xylitol, ethanol, or 2-butanol
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-
?
additional information
?
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No activity with glucose, arabinose, xylose, acetaldehyde, and 2-butanone
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-
-
additional information
?
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No activity with glucose, arabinose, xylose, acetaldehyde, and 2-butanone
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-
additional information
?
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No activity with glucose, arabinose, xylose, acetaldehyde, and 2-butanone
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-
-
additional information
?
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No activity with glucose, arabinose, xylose, acetaldehyde, and 2-butanone
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-
-
additional information
?
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Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
mannitol can be produced directly from glucose in a two-step enzymatic process, using a Thermotoga neapolitana xylose isomerase mutant and TmMtDH at 60°C. No activity with glucose, xylose, threonine, arabinose, acetaldehyde, 2-butanone, sorbitol, xylitol, ethanol, or 2-butanol
-
-
?
additional information
?
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Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
TM0298 shows no detectable activity on glucose, xylose, threonine, arabinose, acetaldehyde, 2-butanone, sorbitol, xylitol, ethanol, or 2-butanol
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-
?
additional information
?
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No activity with glucose, arabinose, xylose, acetaldehyde, and 2-butanone
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-
additional information
?
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the enzyme shows no activity with xylitol, inositol, sorbitol, rhamnose, mannose and xylose, and with NADPH and NADP+ as cofactors
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-
?
additional information
?
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no activity with sorbitol, xylitol, mannose, rhamnose, and xylose. The enzyme from Thermotoga neapolitana is dependent on NADH
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-
additional information
?
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the enzyme shows no activity with xylitol, inositol, sorbitol, rhamnose, mannose and xylose, and with NADPH and NADP+ as cofactors
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?