1.1.1.6: glycerol dehydrogenase
This is an abbreviated version!
For detailed information about glycerol dehydrogenase, go to the full flat file.
Reaction
Synonyms
B4100_2156, CglD, dehydrogenase, glycerol, GDH, GDH2, GLD, Gld3, GldA, GLDH
ECTree
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Specific Activity
Specific Activity on EC 1.1.1.6 - glycerol dehydrogenase
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0.01
wild type, crude cell extract, pH not specified in the publication, temperature not specified in the publication
0.049
overexpressing cells, crude cell extract, pH not specified in the publication, temperature not specified in the publication
1.26
101.68
15.7
recombinant His-tagged chimeric enzyme GDH-NOX, pH 11.0, 37°C
2.78
with NADP+ as cosubstrate, at pH 8.6 and 35°C
220
4.7
45
purified recombinant enzyme, oxidation of glycerol, 80°C, pH 7.4
6
-
mutant protein D16N/N19A/E23D/L28M/E30N/R31N/Q45E/S46E/V48L/E49R/F52L/K53T/D54G/V58S/G78V/I79V/T82K/A83S/I88V/G108N/R139S/L142M/N145R/K155Q/L211I/G248S/V256I/H268Y/D317E/P319L, pH 9.5, 30°C
6.8
-
mutant protein A15T/D16G/V17A/N19K/E23D/Q45E/S46E/T47M/V48L/E49R/F52L/K53A/V58A/V59A/Q70H/D74N/G78D/E81G/T82N/Q83K/G86T/I88V/G108N/R139S/L142M/N145R/K155Q/V256I/L260M, pH 9.5, 30°C
7.4
-
mutant protein Q70H/D74N/G78D/E81G/T82N/Q83K/C84Y/G86T/I88V/G108N/E134A/E204K/L211I/E215K/I234V/V256I/L260M/E291D/S300C/A302S/E316G/V318I/A320T/I324L/T344D/P345S, pH 9.5, 30°C
additional information
-
assay development for NADH production by recombinant enzyme in reverse micelles, micelle size optimization, overview
101.68
with NAD+ as cosubstrate, at pH 8.6 and 35°C
cell free extract, recombinant strain (over-expression of gycerol dehydrogenase)
220
recombinant strain (overexpression), crude extract, pH not specified in the publication, temperature not specified in the publication
4.7
wild type, crude extract, pH not specified in the publication, temperature not specified in the publication