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1.1.1.53: 3alpha(or 20beta)-hydroxysteroid dehydrogenase

This is an abbreviated version!
For detailed information about 3alpha(or 20beta)-hydroxysteroid dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.53

Reaction

androstan-3alpha,17beta-diol
+
NAD+
=
17beta-hydroxyandrostan-3-one
+
NADH
+
H+

Synonyms

(R)-20-hydroxysteroid dehydrogenase, 20-hydroxysteroid dehydrogenase, 20beta-HSD, 20beta-HSD type 2, 20beta-HSDH, 20beta-hydroxysteroid dehydrogenase, 20beta-hydroxysteroid dehydrogenase type 2, 20HSD, 3-alpha hydroxysteroid dehydrogenase type 3, 3alpha,20beta-hydroxysteroid:NAD+-oxidoreductase, 3alpha-HSD/CR, 3alpha-HSD3, 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase, carbonyl reductase-like 20beta-HSD, carbonyl reductase-like 20beta-hydroxysteroid dehydrogenase, carbonyl reductase/20beta-hydroxysteroid dehydrogenase A, carbonyl reductase/20beta-hydroxysteroid dehydrogenase B, cortisone reductase, CR/20beta-HSD, CR/20beta-HSD A, CR/20beta-HSD B, dehydrogenase, 20beta-hydroxy steroid, delta4-3-ketosteroid hydrogenase, desE, NADH-20beta-hydroxysteroid dehydrogenase, retinol dehydrogenase 2, RoDH2, SDR12C55

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.53 3alpha(or 20beta)-hydroxysteroid dehydrogenase

Cloned

Cloned on EC 1.1.1.53 - 3alpha(or 20beta)-hydroxysteroid dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
85% amino acid sequence homology with human carbonyl reductase
-
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expression in Cos-1 cells
expression in Escherichia coli
heterologously overexpressed in Escherichia coli
-
native and insertion protein, expressed as His-tag fusion protein in Escherichia coli XL1-blue and BL21
-
selenomethionine-substituted mutant enzyme I6T/V47M/T69K, expression in inclusion bodies in Escherichia coli B834(DE3)
subcloned in Escherichia coli strain BL21
-