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1.1.1.44: phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

This is an abbreviated version!
For detailed information about phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating), go to the full flat file.

Word Map on EC 1.1.1.44

Reaction

6-phospho-D-gluconate
+
NADP+
=
D-ribulose 5-phosphate
+
CO2
+
NADPH
+
H+

Synonyms

6-GPD, 6-P-gluconate dehydrogenase, 6-Pgd, 6-PGDH, 6-PGDHase, 6-phospho-D-gluconate dehydrogenase, 6-phospho-D-gluconate-NADP+ oxidoreductase, decarboxylating, 6-phosphogluconate dehydrogenase, 6-phosphogluconate dehydrogenase (decarboxylating), 6-phosphogluconate dehydrogenase 1, 6-phosphogluconate dehydrogenase Gnd1, 6-phosphogluconate dehydrogenase, decarboxylating, 6-phosphogluconate-dehydrogenase, 6-phosphogluconate:NADP oxidoreductase, 6-phosphogluconic carboxylase, 6-phosphogluconic dehydrogenase, 6-phosphonogluconate dehydrogenase, 6PDH, 6PG DH, 6PGD, 6PGDH, 6PGDH/Gnd1, 6PGDH1, 6PGDH2, 6PGDH3, At1G64190, At3g02360, At5g41670, D-gluconate-6-phosphate dehydrogenase, GCG1, gnd, GND1, Gnd1p, Gnd2p, GNTZII, LlPDH, Moth_1283, Os6PGDH1, Os6PGDH2, p6PGDH, peroxisomal 6-phosphogluconate dehydrogenase, Pgd, PGD1, PGD2, PGD3, phosphogluconic acid dehydrogenase, TM0438, YpjI, zwf3

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.44 phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

Crystallization

Crystallization on EC 1.1.1.44 - phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
complexed with substrate, substrate/NADPH and glucose, sitting drop vapour diffusion method, using 0.1 M sodium citrate dihydrate buffer (pH5.4), 0.5 M ammonium acetate, 6-7% (w/v) PEG 3350, and 17-18% (w/v) PEG 4000 as a reservoir, at 18°C
6-phosphogluconate dehydrogenase complexed with 6-phosphogluconate, hanging drop vapour diffusion method, using 0.2 M lithium sulfate, 2.2 M ammonium sulfate or 0.2 M ammonium acetate, 2.2 M ammonium sulfate
-
complexed with substrate, substrate/NADPH and glucose, sitting drop vapour diffusion method, at 4°C, with 0.12 M diammonium hydrogen citrate buffer (pH 5.0) and 20% (w/v) polyethylene glycol (PEG) 3350
crystal structures of recombinant Lactococcus lactis 6-phosphogluconate dehydrogenase in complex with substrate, cofactor, product and inhibitors
crystals are grown at 22 °C in hanging drops or sitting drops with the vapor diffusion technique. X-ray structures of the enzyme in native form as well as in complex with 6-phosphogluconate or NADP+ at resolutions of 2.8 A, 1.9 A, and 2.9 A. A flexible loop close to the active site rearranges upon binding of 6-phosphogluconate and likely regulates the conformation of the cofactor NADP
crystals are grown at 22°C in hanging drops with the vapor diffusion technique. X-ray structures of Pf6PGD in native form as well as in complex with 6-phospho-D-gluconate or NADP+ at resolutions of 2.8 A, 1.9 A, and 2.9 A, respectively
hanging drop vapour diffusion method at 15°C, crystal structure of 6-phosphogluconate dehydrogenase determined at 2.37 Å resolution by molecular replacement